Superoxide Dismutase와 Ascorbate Peroxidase가 엽록체내로 동시에 과대발현된 형질전환 담배의 제초제들에 대한 항산화 반응

김진석,이병회,권석윤,김윤희,김소희,조광연,Kim Jin-Seog,Lee Byung-Hoi,Kwon Suk-Yoon,Kim Yun-Hee,Kim So-Hee,Cho Kwang-Yun 한국식물생명공학회 2005 식물생명공학회지 Vol.32 No.2

Superoxide dismutase와 ascorbate peroxidase가 동시에 과대발현된 담배(CA)를 가지고 여러 제초제들에 대한 항산화 반응을 조사하였다. 온실조건 실험에서, CA 담배는 PSI 저해제로 알려진 paraquat처리에 대해서 내성이 인정되었고 그 정도는 40% 내외였다. PS II 저해제 (bromoxynil, diuron, bromacil), 엽록소 생합성 저해제 (oxyfluorfen), 카로티노이드 생합성 저해제 (fluridone)와 EPSP synthase 저해제 (glyphosate) 처리에서는 CA와 wild type간의 반응차이가 관찰되지 않았다. Paraquat와 diuron을 이용한 약광 조건의 실험에서도 온실조건의 실험결과와 유사한 정도로 paraquat처리에 대해서만 내성을 나타내었다. 온실조건에서의 엽위별 반응의 경우, 6 - 9 엽기 식물체에 paraquat를 처리하였을 때, 약제처리 당시 위로부터 3 - 4번째 전개되고 있었던 잎이 상대적으로 내성 정도가 높게 나타났다. 한편 paraquat 처리 시에 여러 농도의 ascorbic acid를 혼합할 경우, CA와 wild type 모두에서 비슷한 정도로 paraquat 활성을 경감시켰다. 결론적으로, CuZnSOD/APX의 과대발현은 photosystem I 에서 발생되는 산화스트레스에 대해서만 주로 작용하며, 다른 제초제들에 의해 발생되는 산화적 스트레스에 대해서는 소거 능력이 부족한 것으로 판단되었다. Antioxidative responses of transgenic tobacco plants expressing both superoxide dismutase (SOD) and ascorbate peroxidase (APX) in chloroplasts was investigated with several herbicides. In greenhouse test, tolerance of SOD/APX-overexpressed tobacco (CA) to photosystem (PS) I inhibitor paraquat was increased by about 40%. However, any response differences between CA and wild type (WT) tobacco was not observed in a treatment with PS II inhibitors (bromoxynil, diuron and bromacil), chlorophyll biosynthesis inhibitor(oxyfluorfen), carotenoid biosynthesis inhibitor (fluridone) and 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase inhibitor (glyphosate). This tendency was also similar in the growth chamber test of low light intensity, using paraquat and diuron. That is, increased antioxidant activity of CA was shown only in paraquat treatment. When paraquat was foliar-treated to 6 to 9-leaf stage plant, the third to fourth placed leaf from shoot tip showed relatively higher antioxidant activity. Ascorbate supplemented to paraquat solution alleviated the phytotoxicity with a similar range in both CA and WT. In conclusion, CA specifically responded to oxidative stress induced by paraquat among tested herbicides in a whole plant assay.

측두하악관절과 저작근의 통증이 긴장성 두통에 미치는 영향

김진석,어규식,이진용,홍정표,전양현,Kim, Jin-Suk,Auh, Q-Schick,Lee, Jin-Yong,Hong, Jung-Pyo,Chun, Yang-Hyun 대한안면통증구강내과학회 2006 Journal of Oral Medicine and Pain Vol.31 No.4

Generally, Tension-Type Headache(TTH) patients exhibit muscle pain, but can also have TMJ pain, which includes mouth opening limitation or joint sounds. The purpose of our study is to observe the clinical pain characteristics between TTH patients with muscle pain and TMJ pain. One hundred sixty-seven patients were diagnosed with TTH according to the questionnaires based on the International Headache Society's proposal on the diagnostic criteria of TTH. The patients were classified into three group; arthralgia group (18 patients), myalgia group (50 patients) and arthromyalgia group (99 patients). TTH patients with pericranial muscle pain were classified in the myalgia group. TTH patients with temporal region pain were classified in the arthralgia group. TTH patients with both types of pain were classified in the arthromyalgia group. The parameters in the diagnostic criteria such as quality, intensity, laterality of pain, and aggravation due to physical activities were compared among the three groups. 1. There were no significant differences in the quality of pain among the three groups. 2. There were no significant differences in the intensity of pain among the three groups. 3. There were no significant differences in the laterality of pain among the three groups. 4. A higher percentage of patients in the arthromyalgia group experienced headaches that were aggravated due to physical activity (p=0.03) compared to the other groups. The results of this study show that TTH patients with both arthralgia (TMJ pain) and myalgia (pericranial muscle pain) are more aggravated by physical activity than TTH patients with either one.

The effects of selenium on fetal growth and development in CD-1 mice exposed with mercury for the gestation period

김진석,이상목,최석화,이원창,Kim, Jin-suk,Lee, Sang-mok,Choi, Seok-wha,Lee, Won-chang The Korean Society of Veterinary Science 1994 大韓獸醫學會誌 Vol.34 No.2

기형발생을 나타내는 수은의 독성에 대하여는 이미 보고된 바 있으나 임신중에 폭로된 수은의 독작용을 완화시키거나 치료할 수 있는 제재들에 대하여는 별로 알려진 바가 없다. 수은에 대한 셀레늄의 치료적인 상호작용에 대하여는 이미 보고된바 있으나 태아에 대한 효과를 평가하기 위하여 이 연구는 임신한 CD-1마우스에 20ppm 농도의 유기수은을 음수를 통하여 투여한후 동시에 sodium selenite(체중 kg당 0.1mg, 0.2mg, 0.3mg, 피하주사) 또는 BAL(체중 kg당 5.0mg, 피하주사)을 임신 6일째부터 15일 사이에 단독 또는 병용 투여한 후 임신 18일째에 제왕절개술을 실시하였다. 수은단독 투여군에서는 태아의 체중 및 두부-둔부의 길이 그리고 태반의 무게가 투여를 하지 않은 대조군에 비하여 유의성있게 감소하였고 태아의 사망/재흡수 발생과 기형발생(특히 구개열)이 현저히 증가하였다. 셀레늄과 BAL의 단독 또는 병용투여는 수은에 의하여 발생된 태아에 대한 독작용을 억제하거나 감소시키고 기형발생 역시 유의성있게 감소시켰으나 투여용량 그리고 투여계획과는 특이한 상관관계가 발견되지 않았다. 모체의 수은중독 결과로 보이는 모체의 음수, 사료섭취량과 모체의 상대적 기관(간장, 신장, 비장) 무게의 감소도 셀레늄과 BAL의 투여로 증가되었다. 이 연구 결과는 세레늄을 단독 또는 BAL과 같은 기존치료제와 함께 투여할 경우 수은에 의한 기형발생이나 모체의 아급성 중독 증세를 감소시키거나 완화시킬수 있는 효과가 있음을 보여주고 있다. Teratogenic and embryotoxic effects of mercury have been reported, however, there is little information about possible antidotes against mercury exposure during gestation. In order to evaluate therapeutic effects of selenium as an antidote against mercury poisoning, pregnant CD-1 mice were exposed to methylmercury chloride(20ppm) through the drinking water with treatment of sodium selenite (1.0mg, 2.0mg or 3.0mg/kg b.w., subcutaneously) or BAL(5.0mg/kg b.w., subcutaneously) under the single or combination base as the therapeutic agents from day 6 to 15 of gestation. Fetal growth parameters such as body weight and crown-rump length in the mice exposed to mercury, were reduced as was placental weight compared to those in the control. Treatment of selenium(alone, combination with BAL) reduced the harmful effects induced by mercury on the fetal growth parameters even though no specific relationship between dose and therapeutic effect. The incidence of dead fetuses/resorptions and malformed fetuses(especially cleft palate) was also increased in the mercury only treated group. Selenium treatment demonostrated reduced the incidence of abnormal fetuses under the exposure of mercury. Relative maternal organ weights(liver, kidney, spleen) were increased significantly but relative brain weight was decreased as evidenced by decreased in the mercury treated mice compared to that in the control. A subtle indication of maternal mercury toxicity evidenced by changes of relative maternal organ weights, decreased water and feed consumption were also prevented efficiently by selenium treatment. The present study suggests that methylmercuric chloride is embrytoxic and teratogenic in CD-1 mice when exposured during organogenesis and that selenium administration may have therapeutic application for the treatment of mercury poisoning although more applicable study in human should be performed with caution in the future.

유한 이층 영상평면 구조에 의한 EMI 차폐성능 분석

김진석,윤재훈,이애경,김남,Kim, Jin-Suk,Yun, Jae-Hoon,Lee, Ae-Kyung,Kim, Nam 한국통신학회 2000 韓國通信學會論文誌 Vol.25 No.5

영상평면을 사용하면 인쇄회로기판(PCB)의 전자파 복사를 효과적으로 감소시킬 수 있다. 이 논문은 이층 유한 영상평면이 PCB 로부터 방출하는 불요 전자파에 미치는 영향을 분석한 것이다. 유한 영상평면 구조의 해석을 삼각형 patch 모델을 모멘트 기법에 적용하여 이루어졌다. 기존에 보편적으로 사용하던 삼각형 분할방법을 개선하여 표면의 경계에 있는 삼각형에서 두 개의 기저함수를 삼각형 분할방법을 제안하고 그 결과가 수치해석의 정확도에 미치는 영향을 비교분석하였다. EMI problem is very important in a printed circuit board design. This paper deals with image plane as one of the most effective method to reduce EMI in PCB. The shielding performance of one and two layers of image planes of finite width and length is analyzed by the method of moments based on the triangular surface patch model. The current distributions were modeled as two simple line sources. It is demonstrated that proper triangulation should be made so as to take two basis functions in the boundary triangles of the surface.

The effect of thiamin on fetal growth and development in CD-1 mice exposed with mercury for the gestation period

김진석,최석화,Kim, Jin-suk,Choi, Seok-wha The Korean Society of Veterinary Science 1994 大韓獸醫學會誌 Vol.34 No.1

수은에 의한 기형발생은 이미 밝혀졌으나 이에 대한 치료가능 약제들의 효과에 대하여는 아직 밝혀진바 없다. 임신한 CD-1마우스에 20ppm의 수은(methylmercuic chloride)을 음수를 통해 임신 6일째 부터 15일 사이에 투여하고 이와 동시에 투여계획에 따라 기존 치료제 BAL과 티아민(thiamin)을 피하로 투여한 후 임신 18일째에 제왕절개술을 실시하였다. 티아민(200mg/체중 kg)과 BAL(5.0mg/체중 kg) 그리고 티아민과 BAL의 병용치료군의 태아는 체중과 두부-둔부 길이 그리고 태반의 무게가 수은 단독 투여군에 비하여 유의성 있게 무겁거나 길어 대조군에서 보인 수치에 가까왔다. 죽은 태아/재흡수율과 기형인 태아 발생율은 티아민과 BAL 치료군에서 감소되었으며 투여용량이 높을수록 발생율이 낮았다. 또한 높은 농도의 티아민과 BAL은 어미 마우스의 사료 및 음수섭취량을 증가시켰으며 간의 상대적 무게도 증가 시켰다. 이 연구 결과는 티아민을 단독 또는 키레이트제재와 병용투여할 경우 수은에 의한 기형발생을 감소시키거나 방지 할 수 있는 효과가 있음을 보여주고 있다. Pregnant CD-1 mice were exposed to methylmercury in the drinking water at concentration of 20ppm with subcutaneous treatment of thiaminHCl(vitamin $B_1$) (100mg, 200mg or 300mg/ kg b.w.) or BAL(5.0 mg/kg b.w.) under the alone or combined base at the therapeutic agents from day 6 to 15 of gestation. Fetal growth parameters, including body weight and crown-rump length in the mice exposed to mercury, were reduced as placental weight compared to those in the control group(no treatment). The incidence of dead fetuses/resorption and malformed fetuses(especially cleft palate) was also increased even in the group treated with thrapeutic agents as well as in the mercury only treated group. However, all kinds of alteration indicated above, possibly induced by mercury, reduced/or decreased significantly compared to those of control. A subtle indication of maternal toxicity was noted in most experimental animals as evidenced by decreased water consumption and increased relative liver weight. The present study confirmed that methylmercuric chloride is embrytoxic and teratogenic in CD-1 mice when administered during organogenesis and that thiamin administration may have therapeutic application for the treatment or prevention against of deleterious effects induced by mercury during gestation period.

유한 도전성 영상 평면이 PCB 전자파 복사에 미치는 영향 분석

김진석,조광윤,김남,Kim, Jin-Suk,Cho, Kwang-Yun,Kim, Nam 한국통신학회 1998 韓國通信學會論文誌 Vol.23 No.4

본 논문에서는 전류 라인 아래, 위에 놓은 유한 도전성 다층 영상 평면이 전자파 복사에 미치는 영향을 분석하였으며, 전류 라인과 영상 평면의 폭, 전류 라인과 평면의 거리 등의 함수로 영상 평면의 EMI 차폐성능을 계산하였다. 영상 평면에 유도된 전류분포는 모멘트 기법을 이용하여 전계 적분방정식을 풀어서 계산하였으며, 전류분포를 작은 매트릭스 크기로 효율적으로 구하기 위해 전류분포의 변화를 고려하여 적분구간을 분할하는 새로운 방법을 제안하고 그 결과를 다른 방법과 비교하였다. In this ppaer, the confguration of multi-layers of the image planes surrounding the current trace of inite width has been considered, and the shielding performance of image planes is analyzed with the parameters of the width and conductivity of the planes, and the distance between the current trace and the planes. The induced current distributions on the image planes have bgeen calculated by solving a set of electric field integral equations by the method of moments. To cacluate the current distribution on the planes effectively, a new method for dividing integral intervals is proposed.

Glyphosate에 대한 옥수수 반응의 개선된 검정방법

김진석,이병회,김소희,민석기,최정섭,Kim, Jin-Seog,Lee, Byung-Hoi,Kim, So-Hee,Min, Suk-Ki,Choi, Jung-Sup 한국식물생명공학회 2006 식물생명공학회지 Vol.33 No.1

본 연구에서는 옥수수를 대상으로 glyphosate에 대한 여러 가지 생리적 반응을 검토한 후, glyphosate 저항성 평가에 활용될 수 있는 보다 개선된 방법 두 가지를 확립하였다. 한 가지 방법은 옥수수 제3엽 상단에 약제를 국소 처리한 다음, 처리 후 3일째에 약제처리 되지 않았던 제4엽의 신장 정도를 조사하는 것이다 (전식물체-엽생장 검정). 이 경우 glyphosate $50-1,600{\mu}g/mL$ 범위에서 농도가 증가됨에 따라 엽 생장이 억제되었으며, $1,600{\mu}g/mL$ 농도에서의 생장 억제율은 무처리 대비 55.5%였다. 다른 한 가지 방법은 옥수수 제3본엽의 엽절편 ($4{\times}4mm$) 4개씩을 $200{\mu}L$의 시험용액이 담긴 48 well plate에 치상한 후 $25^{\circ}C$ 연속 명조건에 24시간동안 배양하여 shikimate 축적량을 조사하는 것이다 (엽절편-shikimate 축적 검정). 이 경우 시험용액에 0.33% sucrose를 가하면 무첨가에 비해 약3-4배 정도의 shikimate 축적 증가가 관찰되었고 glyphosate $2-8{\mu}g/mL$ 농도범위에서 직선적 증가반응을 나타내어 기존방법 (Shaner et al. 2005)보다 개선된 특징을 보였다. 본 방법들은 glyphosate 저항성 옥수수를 창출할 때 또는 저항성 유전자의 타 식물로의 이동여부와 잡초화된 저항성 옥수수 존재여부를 감별하는데 활용될 수 있을 것이다. 이 때 glyphosate에 대한 저항성 원인이 작용점 EPSPS와 관련이 있는 경우에는 "엽절편-shikimate 축적 검정"이 가장 바람직하고, 저항성 원인이 체내이행 감소 때문일 경우에는 "전식물체-엽생장 검정" 수행이 필요하다. Several methods for determining the response of corn to glyphosate were investigated to provide a fast and reliable method for identifying glyphosate-resistant corn in vivo. Two bioassays were developed. One assay is named 'whole plant / leaf growth assay', in which the herbicide glyphosate is applied on the upper part of 3rd leaf and the growth of herbicide-untreated 4th leaf is measured at 3 day after treatment. in this assay, the leaf growth of conventional corn was inhibited in a dose dependent from 50 to $1600{\mu}g/mL$ of glyphosate and growth inhibition at $1600{\mu}g/mL$ was 55% of untreated control. The assay has the potential to be used especially in the case that the primary cause of glyphosate resistance is related with a reduction of the herbicide translocation. Another assay is named 'leaf segment / shikimate accumulation assay', in which the four excised leaf segments ($4{\times}4mm$) are placed in each well of a 48-well microtiter plate containing $200{\mu}L$ test solution and the amount of shikimate is determined after incubation for 24 h in continuous light at $25^{\circ}C$. In this assay, 0.33% sucrose added to basic test solution enhanced a shikimate accumulation by 3 to 4 times and the shikimate accumulation was linearly occurred from 2 to $8{\mu}g/mL$ of glyphosate, showing an improved response to the method described by Shaner et al. (2005). The leaf segment / shikimate accumulation assay is simple and robust and has the potential to be used as a high throughput assay in the case that the primary cause of glyphosate resistance is related with EPSPS, target site of the herbicide. Taken together, these two assays would be highly useful to initially select the lines obtained after transformation, to investigate the migration of glyphosate-resistant gene into other weeds and to detect a weedy glyphosate-resistant corn in field.

차량 궤적 추적을 위한 불확실성 처리기 구현

김진석,김동호,류근호,Kim, Jin-Suk,Kim, Dong-Ho,Ryu, Keun-Ho 한국정보처리학회 2004 정보처리학회논문지D Vol.11 No.5

인터넷 기술의 발달로 다양한 응용분야의 컴퓨팅 환경은 상당한 변화를 겪어 왔다. 특히 지난 3년여 동안 물류분야에서는 물류 회사간 인터넷 기반의 가상 비즈니스 활동 또는 서비스 아키텍처를 의미하는 e-로지스틱스라는 새로운 개념에 대한 활발한 연구가 진행되었다. 효과적인 e-로지스틱스 프레임워크 구축을 위해서 GIS, GPS 및 시공간 데이터베이스를 포함하는 이동체 기술 개발이 진행 중에 있다. 이동체 기술은 자동차, 비행기, 선박 등과 같이 시간에 따라 공간상의 위치를 변경하는 시공간 객체에 대한 효율적인 데이터 관리를 의미한다. 그러나 기존의 관련 시스템은 여러 가지 이유로 이동체에 대하여 오직 가장 최근에 검출된 위치정보만을 관리하기 때문에, 과거 및 미래의 불확실한 위치 추정방법에 대한 구체적인 제시는 미약한 상태에 있다. 따라서 이 논문에서는 이동체에 대한 이력정보 관리와 이를 이용한 과거의 위치 추정이 가능한 시스템을 제안한다. 이를 위해 물류 운송차량 위치 추적을 위한 모델링과 데이터베이스 및 시스템 구조를 제시한다. 아울러 제안 시스템을 e-로지스틱스 분야의 주요 응용인 택배 차량에 대한 위치정보 불확실성 처리 시나리오에 적용하는 예를 가지고 설명한다. Along the advent of Internet technology, the computing environment has been considerably changed in many application domains. Especially, a lot of researches for e-Logistics have been done for the last 3 years. The e-Logistics means the virtual business activity and service architecture among the logistics companies based on the Internet technology. To construct effectively the e-Logistics framework, researches on the development of the Moving Object Technology(MOT) including GPS and GIS with spatiotemporal databases technique so far has been done The Moving Object Technology stands for the efficient management for the spatiotemporal objects such as vehicles, airplanes, and vessels which change continuously their spatial location along with time flows. However, most systems manage just only the location information detected lately by many reasons so that the uncertainty processing for the past and future location of the moving objects is still very hard. In this paper, we propose the moving object uncertainty model and system design for e-Logistics applications. The MOMS architecture in e-Logistics is suggested and the detailed explain of sub-systems including the uncertainty processor of moving objects is described. We also explain the comprehensive examples of MOMS and uncertainty processing in Delivery Parcel Application that is one of major application of e-Logistics domain.

프롤레타리아 문학의 여성지식인 연구 : 한설야의 『황혼』의 려순을 중심으로

김진석(Kim, Jin-suk) 한국언어문학회 2011 한국언어문학 Vol.78 No.-

This paper has examined the characteristics of female intellectuals in proletarian literature, focused on Ryeo-sun in Han Seol-ya’s Hwang-Hon ‘sunset.’ What has been discussed can be summarized as follows. From the viewpoint of socialism, laborers and women fall within the class that is representatively exploited in capitalist society. As such, socialism has recognized women as sharing the same destiny with laborers in the same dimension as the class liberation movement. Consequently, the problem of women has been given focus from the perspective of struggle for action rather than dealt with on the side of their nature including identification of gender. The first half of the work Sunset is focused on the triangular relationship among Gyeong-jae, Ryeo-sun, and Hyeon-ok. Ryeo-sun turns out to be a victim in her love narrative. Her expectation for Gyeong-jae serves as a chance to recognize the Bourgeois’ egoism. Through the course of this narrative, she gives up her unilateral relationship with Gyeong-jae and establishes an independent relationship, which she achieves by declaring to quit against his egoistic desire for maintaining his meaningless love. This is the starting point from which she has seen humans and society from the viewpoint of classism. Ryeo-sun, as she is, is clearly distinguished from the female figures in previous pro-literary works. For her, the ordeal is an object for overcoming rather than for frustration. Such a rational attitude is closely related to intellectual training. However, this is far from being a figure crystallized on the aesthetic side though it can be an educational model for socialist revolution. In this sense, the love aspects of Sunset has not only a new possibility of pro-literature but also certain problems to overcome simultaneously. The last half of the work reveals a tendency to emphasize the necessity of class struggle. This is crystallized through the opposition and conflict between employers and employees. During the course, Ryeo-sun’s classist shift to laborer implies complex meanings. She realizes contradictions of Capitalism through her labor experiences. Here, the struggle against Bourgeois reduces to the problem of knowledge rather than of assets or capital. This means that the gist of pro-literature lies in the education for awakening the sleepy awareness of the proletariat. The fact that Ryeo-sun has received higher education serves as a crucial social force in the course of the struggle against An Jung-seo. The education works as an important element in the recognition of ego and society. Furthermore, it comprises a theoretical basis for emphasis on the necessity of class struggle. This means that the success of labor movement is directly connected with the education of laborers. The fruit of her education is crystallized by labor disputes. Through them, Y Spinning Mill happens to be turned from a lethargic field of labor into the space of struggle for labor liberation. Thus, the emergence of an intellectual female like Ryeo-sun can be evaluated to have open a new horizon for pro-literature despite the novel’s artificial structure.

이종관 저 『사이버 문화와 예술의 유혹』 문예출판사, 2003

김진석(Jin Suk Kim) 철학연구회 2003 哲學硏究 Vol.62 No.-