갑상선에서 protein kinase C에 의한 thyroxine 유리조절

김진상,Kim, Jin-shang 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.6

Previous studies suggested that the inhibition of thyroxine ($T_4$) release by ${\alpha}_1$-adrenoceptor and muscarinic receptor stimulation results in activated protein kinase C (PKC) from mouse and guinea pig thyroids. In the present study, the effect of carbachol, methoxamine, phorbol myristate acetate (PMA), and R59022 on the release of $T_4$ from the mouse, rat, and guinea pig thyroids was compared to clarify the role of PKC in the regulation of the release of $T_4$. The thyroids were incubated in the medium containing the test agents, samples of the medium were assayed for $T_4$ by EIA kits. Forskolin, an adenylate cyclase activator, chlorophenylthio-cAMP sodium, a membrane permeable analog of cAMP, and isobutyl-methylxanthine, a phosphodiesterase inhibitor, like TSH (thyroid stimulating hormone), enhaced the release of $T_4$ from the mouse, rat, and guinea pig thyroids. Methoxamine, an ${\alpha}_1$-adrenoceptor agonist, inhibited the TSH-stimulated release of $T_4$ in mouse, but not rat and guinea pig thyroids. In contrast, carbachol, a muscarinic receptor agonist, inhibited the release of $T_4$ in guinea pig, but not mouse and rat thyroids. These inhibition were reversed by prazosin, an ${\alpha}_1$-adrenoceptor antagonist or atropine, a muscarinic antagonist or $M_1$- and $M_3$-muscarinic antagonists, in mouse or guinea pig thyroids. In addition, staurosporine, a PKC inhibitor, reversed methoxamine or carbachol inhibition of TSH stimulation. Furthermore, PMA, a PKC activator, and R59022, a diacylglycerol (DAG) kinase inhibitor, inhibited the TSH-stimulated release of $T_4$ in mouse, rat, and guinea pig thyroids. These inhibition were blocked by staurosporine. These findings suggest that the activation of receptor or DAG inhibits TSH-stimulated $T_4$ release through a PKC-dependent mechanism in thyroid gland.

흰쥐에서 대사작용 억제에 의한 혈중 Mg²⁺ 조절

김종식,김상진,김진상,Kim, Jong-shick,Kim, Shang-jin,Kim, Jin-shang 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.1

Magnesium ($Mg^{2+}$) plays an important role in the regulation of a range of intracellular processes. Regulation of extracellular $Mg^{2+}$ contents was studied in the anesthetized Sprague-Dawley (SD) rats. Animals were injected intraperitoneally with sodium nitrite ($NaNO_2$), and circulating $Mg^{2+}$($[Mg^{2+}]c$) was measured after the injection and then 10 and 20 minutes later. A dose-dependent increase in $[Mg^{2+}]c$ was observed in animals injected with $NaNO_2$ at a dose of 10mg/kg or higher. Pretreatment with methylene blue prevented the $NaNO_2$-induced increase in $[Mg^{2+}]c$. $[Mg^{2+}]c$ displayed an inverse linear correlation with hemoglobin and exponential correlation during $NaNO_2$ injection. Injection of KCN or rotenone also induced an increase in $[Mg^{2+}]c$. An increase in $[Mg^{2+}]c$ was observed when respiration rate was reduced from 100/min (140ml/min) to 10/min (14ml/min) during 30 min. These results indicate that changes in $[Mg^{2+}]c$ inversely reflect alteration of ATP in a model of metabolic inhibition.

흰쥐 대동맥에서 Trazodone의 혈관이완 작용기전

김상진,김정곤,김진상,Kim, Shang-jin,Kim, Jeong-gon,Kim, Jin-shang 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.4

The aim of this study was to investigate trazodone's effect on vasorelaxation and blood pressure lowering and to examine its underlying mechanism of action in isolated thoracic aorta and anesthesized rats. Precontracted aortic rings with high KCl were relaxed with trazodone, at concentrations of $50{\mu}M$ or greater. However, precontracted rings with phenylephrine (PE) were relaxed with trazodone, at concentrations of $0.03{\mu}M$ or greater, in a concentration-dependent manner. These relaxant effects of trazodone on endothelium intact rat aortic rings were significantly greater than those on denuded rings. The trazodone-induced relaxations were suppressed by nitric oxide synthase (NOS) inhibitors, N(G)-nitro-L-arginine (L-NNA) and N(omega)-nitro-L-arginine methyl ester (L-NAME), guanylate cyclase inhibitors, methylene blue and 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), a $Ca^{2+}$-activated $K^+$ channel blocker, tetrabutylammonium (TBA), a $Ca^{2+}$ channel blocker, nifedipine, $Na^+$ channel blockers, lidocaine and procaine, and removal of extracellular $Na^+$, but not by aminoguanidine, 2-nitro-4-carboxyphenyl-n, n-diphenylcarbamate (NCDC), indomethacin, glibenclamide and clotrimazole. In vivo, infusion of trazodone elicited significant decrease in arterial blood pressure. Trazodone-induced decrease in blood pressure was markedly inhibited by pretreatment of intravenous injection of saponin, L-NNA, methylene blue, TBA, lidocaine or nifedipine. These findings suggest that the endothelium-dependent relaxation and decrease in blood pressure induced by trazodone is mediated by release of NO from the endothelium, activation of TBA-sensitive $Ca^{2+}$-activated $K^+$ channels or inhibition of $Ca^{2+}$ entry through voltage-gated channel.

Microencapsulation 헤모글로빈 인공적혈구의 용혈

김민호 ( Min Ho Kim ),강형섭 ( Hyung Sub Kang ),김진상 ( Jin Shang Kim ),백병걸 ( Byeong Kirl Baek ),홍철운 ( Chul Un Hong ),김성종 ( Seong Jong Kim ),신형식 ( Hyung Shik Shin ),김기범 ( Gi Beum Kim ) 한국조직공학과 재생의학회 2010 조직공학과 재생의학 Vol.7 No.2

The purpose of this study was to reduce the hemolysis of artificial red blood cells by extracting phospolipids from egg yolk through microencapsulation to evaluate the oxygen transfer rate of artificial red blood cells in an intravascular artificial lung device. In this study, hemosome was produced to encapsulate hemoglobin which extracted the red blood cell of bovine blood with phospolipids reduced egg yolk. The solution blended blood with hemosome at the rate of 4 to 1 and normal blood measured the oxygen transfer rate. The hemolysis of the microencapsulated artificial red blood cell measured less than the normal red blood cell through absorptivity. Due to the phospolipids enclosing the red blood cell to protect it, the produced hemosome operated at a higher oxygen transfer rate by reducing the demolition of hemoglobin. Consequently, hemolysis was observed to advance through micoencapsulation.

자가면역글로불린 G 측정을 위한 표면탄성파 바이오센서에 대한 연구

김기범 ( Gi Beum Kim ),정우석 ( Woo Suk Cheong ),박영란 ( Young Ran Park ),김상진 ( Shang Jin Kim ),김성종 ( Seong Jong Kim ),강형섭 ( Hyung Suk Kang ),김진상 ( Jin Shang Kim ),홍철운 ( Chul Un Hong ) 한국화학공학회 2011 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.49 No.2

In this study, we have developed shear horizontal(SH) surface acoustic wave(SAW) sensors for the detection of immunoglobulin G(IgG) on the gold coated delay line of SH-SAW devices. As the result of the experiment, we could uniformly immobilize anti-MIgG(mouse IgG) conjugate on the surface of gold. When displaying results of immobilization on the surface of gold using G-anti MIgG conjugate and blocking buffer in frequency shift, G-anti MIgG conjugate showed frequency shift of 75.1 kHz in the initial frequency, and blocking buffer showed frequency shift of 215.7 kHz. When various concentrations of MIgG was added in 100 MHz type sensor, the sensor showed 46.3, 127.45, 161.21 and 262.39 kHz frequency shift at 25, 50, 75 and 100 μg MIgG concentration, respectively.

인공 폐 보조장치 내에서의 유체 유동 모델링에 대한 연구

김기범 ( Gi Beum Kim ),박영란 ( Young Ran Park ),김상진 ( Shang Jin Kim ),홍철운 ( Chul Un Hong ),강형섭 ( Hyung Sub Kang ),김진상 ( Jin Shang Kin ),김성종 ( Seong Jong Kim ),김민호 ( Min Ho Kim ) 한국화학공학회 2011 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.49 No.2

In this study, the characteristic of fluid flow in the new type lung assist devices has been established using computational fluid dynamics(CFD). For the modeling, the hollow fiber was ignored, and vertical types and tangential types were used for the model. Which was to analyze the flow characteristics of the fluid Dow model when there exists 1 and 2 input/output pods, and when the input/output ports is located at the center of the cylinder and at the tangential direction with the cylinder wall. The modeling results showed that it was possible to eliminate no-flow region(stagnant layer) as shown in the vertical type when an inlet and an outlet were installed on the tangential direction of the cylinder as shown in the tangential type. Also, in the tangential type, vortex-type flow appeared as dominant, and it showed a complicated flow not deviated to one side. When the number of input/output was two, there was no deviated flow, and complicated flows were generated all across the tube. From the test result, it was found that input/output of flow was tangential type and complicated flows with no stagnant layer would be generated when there are two inputs/outputs, respectively.

혈액대용물질의 제조 및 특성 평가

김기범 ( Gi Beum Kim ),박재관 ( Jai Koan Park ),김성종 ( Seong Jong Kim ),김종수 ( Jong Soo Kim ),유일수 ( Il Soo You ),김민호 ( Min Ho Kim ),홍철운 ( Chul Un Hong ),김진상 ( Jin Shang Kim ),강형섭 ( Hyung Sub Kang ) 한국화학공학회 2008 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.46 No.3

흰쥐에서 혈관내피 의존적인 혈관이완과 혈압하강에 대한 propofol의 억제 효과

김상진,김정곤,조성건,강형섭,김진상,Kim, Shang-Jin,Kim, Jeong-gon,Joe, Sung-gun,Kang, Hyung-sub,Kim, Jin-shang 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.3

We studied the effect of propofol (PPF) on the endothelium-dependent vascular responses in isolated rat thoracic aorta. In aortic rings with endothelium, PPF inhibited the phenylephrine (PE)-induced contraction in a concentration-dependent manner. In PE-precontracted preparations, PPF attenuated the endothelium-dependent relaxation by acetylcholine but not by A23187. And PPF did not attenuate the endothelium-independent relaxation by sodium nitroprusside (SNP). The relaxation induced by acetylcholine in PE-precontracted aortic rings was significantly augmented by zaprinast, a cGMP-specific phosphodiesterase inhibitor, and this augmentation was inhibited by PPF. Although SNP-induced relaxation was significantly augmented by zaprinast, this augmentation was not inhibited by PPF. In preparations preconstricted with PE, the PPF-induced relaxation was inhibited by atropine. In addition, PPF attenuated the vasorelaxation by phosphodiesterase inhibitors (IBMX, Ro20-1724 or zaprinast except milrinone). In vivo, the infusion of acetylcholine and SNP showed decreased arterial blood pressure in rats. The pre-injection of PPF inhibited the acetylcholine-induced blood pressure lowering, but not the SNP-induced blood pressure lowering. These results suggest that PPF can attenuate in part the acetylcholine-induced vasorelaxation and blood pressure lowering through the inhibition of the acetylcholine receptor-mediated endothelium-derived relaxing factor by acting on endothelium. It is considered that the inhibitory effect of PPF on the vasorelaxation is due to the decreased level of cGMP which can be attributed to the inhibition of the muscarinic receptor and/or receptor-G-protein interaction.

CFD 해석을 이용한 Balloon형 인공심폐기 설계를 위한 구조적 해석

박영란 ( Young Ran Park ),심정연 ( Jeong Yeon Shim ),김기범 ( Gi Beum Kim ),김상진 ( Shang Jin Kim ),강형섭 ( Hyung Sub Kang ),김진상 ( Jin Shang Kim ),김민호 ( Min Ho Kim ),홍철운 ( Chul Un Hong ),김성종 ( Seong Jong Kim ) 한국화학공학회 2011 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.49 No.2

In this study, we attempted a structural analysis in order to design a balloon type extracorporeal membrane oxygenator that can induce blood flow without using blood pumps for the purpose of complementing the weakness in the existing extracorporeal membrane oxygenator. To analyze the flow characteristic of the blood flow within the virtual model of extracorporeal membrane oxygenator, computational fluid dynamics(CFD) modeling method was used. The operating principle of this system is to make the surface of the extracorporeal membrane oxygenator keep contracting and dilating regularly by applying pressure load using a balloon, and the ``Time Function Value`` that changes according to the time was applied by calculating a half cycle of sine waveform and a cycle of sine. waveform Under the assumption that the uni-directional blood flow could be induced if the balloon type extracorporeal membrane oxygenator was designed as per the method described above, we conducted a structural analysis accordingly. We measured and analyzed the velocity and pressure of blood flow at both inlet and outlet of the extracorporeal membrane oxygenator through CFD simulation. As a result of the modeling, it was confirmed that there was a flow in accord with the direction of the blood by the contraction/dilation. With CFD simulation, the characteristics of blood flow can be predicted in advance, so it is judged that this will be able to provide the most optimized design in producing an extracorporeal membrane oxygenator.

심방 이뇨호르몬의 분비조절에서 Ca²⁺이 protein kinase C 활성화에 미치는 영향

강창원,김진상,이호일,Kang, Chang-won,Kim, Jin-shang,Lee, Ho-il 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.5

Atrial natriuretic peptide(ANP) is a hormone with potent natriuretic, diuretic and relaxing properties on vascular smooth muscle. Specific chemical modulator in response for the ANP secretion has not been found yet. Therefore, we have investigated the role of $Ca^{2+}$ responsible for the regulation of ANP induced by protein kinase C(PKC) on mechanically stretch-induced ANP secretion in the rat atria. The results obtained were as follows ; 1. ANP secretion and ANP concentration were increased to more in $Ca^{2+}$-free buffer than in the Kreb-Henseleit buffer on mechanically stretch-induced ANP secretion(p < 0.05), but extracellular fluid translocation(ECF) was not significant. Phorbol 12-myristate 13-acetate(PMA, $10^{-7}M$) induced ANP secretion and ANP concentration in $Ca^{2+}$-free buffer shown to more accentuate on mechanically stretch-induced ANP secretion than in the $Ca^{2+}$-free buffer(p < 0.05), but ECF translocation was not significant. 2. In the presence of ryanodine($3{\times}10^{-6}M$), PMA($10^{-7}M$) induced ANP secretion and ANP concentration in the Kreb-Henseleit buffer were shown to more increase on mechanically stretch-induced ANP secretion than in the ryanodine($3{\times}10^{-6}M$) with the Kreb-Henseleit buffer(p < 0.05), but ECF translocation was not significant. 3. In the presence of ryanodine($3{\times}10^{-6}M$), PMA($10^{-7}M$) induced ANP secretion and ANP concentration in the $Ca^{2+}$-free buffer was shown to more increase on mechanically stretch-induced ANP secretion than in the ryanodine($3{\times}10^{-6}M$) with the $Ca^{2+}$-free buffer on mechanically induced ANP secretion(p < 0.05), but ECF translocation was not significant. The results suggest that PKC-induced ANP secretion may not be related to the change of $Ca^{2+}$ on mechanically induced ANP secretion in the rat atria.