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김주현,김상준,김강민,심채경,손동훈,KIM JOO HYEON,KIM SANG JOON,KIM KANG-MIN,SIM CHAE KYUNG,SON DONG HOON 한국천문학회 2004 天文學論叢 Vol.19 No.1
We have carried out the high-resolution spectroscopic observations of Titan using BOES (Bohyunsan Echelle Spectrometer) to establish a standard spectral atlas of Titan. The observations were made on November 3, 4, 16 and 17, 2003 using a 300 11m fiber optics with a spectral resolving power of 30,000. The spectral atlas will be served as a reference for the future spectroscopic researches of Titan and other planetary objects. We were able to confirm $CH_4$ absorption lines of the Kuiper bands, and find unidentified lines near $7500{\AA}$.
김주현,류청매,스리니바산 사티야라지,김명겸,김상용,위지향,임완택,Kim, Ju-Hyeon,Liu, Qing-Mei,Srinivasan, Sathiyaraj,Kim, Myung Kyum,Kim, Sang Yong,Wee, Ji-Hyang,Im, Wan-Taek The Microbiological Society of Korea 2018 미생물학회지 Vol.54 No.4
A Gram-positive, rod-shaped, ivory colored, and motile, Lactobacillus koreensis 26-25 was isolated from Korean kimchi. Strain 26-25 showed the ability of conversion from major ginsenosides into minor ginsenosides for which whole genome was sequenced. The whole genome sequence of Lactobacillus koreensis 26-25 consisted of one circular chromosome comprised of 3,006,812 bp, with a DNA G + C content of 49.23%. The whole genome analysis of strain 26-25 showed many glycosides hydrolase genes, which may contribute to identify the genes responsible for transformation of major ginsenosides into minor ginsenosides for its high pharmacological effects. 김치로부터 분리한 Lactobacillus koreensis 26-25 균주의 유전체서열을 분석하였다. 균주 26-25의 유전체는 G + C 비율이 49.23%이며, 2,720개의 유전자와 2,556개의 단백질 코딩 유전자, 85개의 위유전자 그리고 78개의 RNA 유전자를 포함한 단일 원형 염색체로 구성되었으면 그 크기는 3,006,812 bp였다. 균주 26-25는 인삼사포닌의 당 분해에 관여하는 여러 타입의 글라이코시다제 유전자를 가지고 있었다. 이러한 지놈 분석은 주요 진세노사이드가 우수한 약리학적 활성의 미량 진세노사이드로 전환하는데 관여하는 유전자 특징을 이해하는데 큰 기여가 되었다.
백색광 LED를 사용한 독서등의 최적 색온도에 따른 사람의 시력 변화 연구
김주현,장원범,이석환,정광교,김동현,김정미,류재준,문성득,이승현,고영수,허산,장미나,정창호,장지호,Kim, Juhyun,Chang, Wonbeom,Lee, Seokhwan,Jung, Kwangkyo,Kim, Donghyun,Kim, Jeongmi,Ryu, Jaejun,Moon, Seongdeuk,Lee, Seunghyun,Ko, Youngsu,Huh, Sa 한국전기전자재료학회 2013 전기전자재료학회논문지 Vol.26 No.1
White light emitting diode (LED) determined the most appropriate color temperature in reading lighting evaluated fatigue degree of eye according to color temperature. The eye fatigue degrees are determined by brightness and color temperature. Therefore, we measured the results of eyes test according to the change of color temperature and brightness. Experiments except for astigmatic corrected visual acuity of 0.8 more and age 20 to 25 years old, male and female college students was conducted in 100 patients. And constant illumination conditions, visual acuity was measured by varying the color temperature. The optometry at 10 minutes in the darkroom adapted eye. And then the temperature of $25{\pm}3$ degrees, the humidity was carried out at $50{\pm}5%$. As a result of typical color temperature of white light (5,600 K) has identification of the readability.
김주현,최한고,Kim, Ju-Hyeon,Choi, Han-Go 한국융합신호처리학회 2014 융합신호처리학회 논문지 (JISPS) Vol.15 No.4
본 연구는 칼라기반에서 단일 이동객체 추적을 다루고 있다. 우선 매 영상에서 이동객체 영상의 밝기 변화에 따른 추적 약점을 개선하기 위해 기존의 Camshift 알고리즘을 보완하였다. 보완된 알고리즘도 추적중인 물체와 색상이 같은 주변 물체가 존재할 경우 불안정한 추적을 보여주었는데 본 연구에서는 이를 해결하기 위해 Optical Flow기반의 KLT 알고리즘과 병합하는 방법을 제시하였다. 픽셀기반의 특징점 추적을 수행하는 KLT 알고리즘은 칼라기반의 Camshift의 단점을 보완할 수 있다. 실험 결과 제안된 병합 방법은 기존의 추적단점을 보완하였으며 추적성능이 개선됨을 실험으로 확인하였다. This paper deals with a color-based tracking of a moving object. Firstly, existing Camshift algorithm is complemented to improve the tracking weakness in the brightness change of an image which occurs in every frame. The complemented Camshift still shows unstable tracking when the objects with same color of the tracking object exist in background. In order to overcome the drawback this paper proposes the Camshift combined with KLT algorithm based on optical flow. The KLT algorithm performing the pixel-based feature tracking can complement the shortcoming of Camshift. Experimental results show that the merged tracking method makes up for the drawback of the Camshit algorithm and also improves tracking performance.
C형 간염바이러스(HCV) 유전체를 특이적으로 변형할 수 있는 Trans-Splicing Aptazyme 발굴
김주현,이창호,장선영,이성욱,Kim, Ju-Hyun,Lee, Chang-Ho,Jang, Sun-Young,Lee, Seong-Wook 한국미생물학회 2008 미생물학회지 Vol.44 No.3
C형 간염바이러스(hepatitis C virus; HCV) 복제를 효과적이며 특이적으로 제어할 수 있는 유전산물을 개발하기 위하여 특정 리간드 존재에 의해 allosteric하게 그 활성이 조절될 수 있는 HCV 유전체 표적 trans-splicing 리보자임(trans-splicing aptazyme)을 발굴하였다. 이러한 trans-splicing aptazyme은 특정 리간드와 특이적으로 결합하는 RNA aptamer 부위, aptamer와 리간드와의 결합에 의해 리보자임 활성을 유도할 수 있도록 구조적 변이를 전달할 수 있는 communication module부위 및 HCV IRES의 +199 nt를 인지하는trans-splicing리보자임 등으로 구성되도록 설계하였다. 특히 trans-splicing 리보자임의 catalytic core의 P6과 P8 부위에 aptamer와 communication module을 삽입하였을 때 가장 allosteric하게 리보자임 활성이 유도되었다. 이러한 리보자임은 리간드가 없거나 대조 리간드가 존재할 때에는 trans-splicing 반응을 유도하지 못하였으나 특정 리간드가 존재할 때에만 효과적이며 특이적으로 trans-splicing 반응을 유도하여 표적 RNA를 변형시킬 수 있음을 관찰하였다. 이러한 aptazyme은 HCV 증식에 대해 특이적이며 효과적인 억제를 위한 선도물질로 이용 가능할 뿐 아니라 HCV 치료선도 물질의 스크리닝용 도구로서도 활용될 수 있을 것이다. For the development of specific and effective basic genetic materials to inhibit replication of hepatitis C virus (HCV), HCV genome-targeting trans-splicing aptazyme, which activity is allosterically regulated by a specific ligand, was developed. The aptazyme was designed to be comprised of sequence of RNA aptamer to the ligand, communication module sequence which can transfer structural transition for inducing ribozyme activity upon binding the ligand to the aptamer, and trans-splicing ribozyme targeting +199 nt of HCV IRES. Especially, when the aptamer and the communication module was inserted at both P6 and P8 catalytic domain of the specific ribozyme, allosteric activity of the aptazyme was the most induced. The aptazyme was shown to induce activity of trans-splicing reaction specifically and efficiently only in the presence of the specific ligand, but neither in the absence of any ligand nor in the presence of control ligand. This aptazyme can be used as a specific and effective genetic agent against HCV, and a tool for the isolation of anti-HCV lead compounds.
지혜 깊어지는 건강 - 우리 아이를 위한 1분 건강 - 척추측만증 겨울방학, 아이의 척추를 점검하라
김주현,Kim, Ju-Hyeon 한국건강관리협회 2011 건강소식 Vol.35 No.12
각종 인터넷 포털 사이트를 검색하면 척추측만증에 대한 설명이 나오는데 대체로 척추측만증은 척추가 옆으로 심하게 굽은 증상. 통증은 없고 서서히 진행되는데 심하면 내장 압박을 비롯하여 여러 가지 장애를 일으키는 병으로 소개되고 있다. 하지만 척추측만증은 단순히 척추가 옆으로만 굽은 것을 말하는 것이 아니라 뒤에서 봤을 때 옆으로 만곡된 것 이외에도 척추 뼈 자체의 회전 변형을 동반한 3차원적인 척추기형을 말한다.
Top-down 방식의 열분해질량분석 스펙트라 분석 및 Gram-type 세균 분류
김주현,Kim, Ju-Hyun 한국군사과학기술학회 2011 한국군사과학기술학회지 Vol.14 No.4
To apply TMAH-based Py-MS to a field biological detection system for real-time classification of cell-type, reproducible patterns of the TMAH-based Py-MS spectra was known as a critical factor for classification but was seriously disturbed by quantity of cells injected into pyro-tube. This factor is an exterior variable that could not be complemented by improving the performance of the TMAH-based Py-MS instrument. One of idea to solve the knotty problem has been flashed from "Top-down proteomics for identification of intact microoganisms". That is, biomarker peaks are selected from complicate Py-MS spectra for intact microoganisms by tracing out their origins, based on Py-MS spectra for the featured components of different cell-types, in Top-down approach. This idea has been tested in classification of different Gram-type microoganisms. Through the analyses of spectra for the featured components - peptidoglycan and lipoteichoic acid for Gram-positive cells and lipopolysaccharide and lipid A for Gram-negative cells - with comparing to the spectra the corresponding Gram-type cells in the Top-down approach, biomarker peaks were selected to carry out PCA(Principal Component Analysis) in order to see classification of different Gram-types, resulting in significant improvement of their classification. Furthermore, weighting biomarker peaks on intact cell's spectra, based on the data for the featured components of the Gram-types, contributed to elevate classification performance.
TMAH에 기반한 열분해 질량분석법의 생물 탐지체계 적용
김주현,Kim, Ju-Hyun 한국군사과학기술학회 2011 한국군사과학기술학회지 Vol.14 No.2
TMAH-based Py-MS has been investigated to apply for a real-time classification of biological agents in the field. Acquiring reproducible data from mass spectrometry is a key to biological detection in the field. Nevertheless, it has been little studied on what factors could affect to the reproducibility of the TMAH-based Py-MS spectrum patterns. Given the TMAH-based Py-MS applied to the field system, several factors which could affect to the reproducible pattern of TMAH-based Py-MS spectra are needed to be examined, including changes in TMAH injection volume, growth temperature for microorganism, and number of cells collected in pyrolyzer, and implication of stabilizer used for lyophilization. This study showed that the reproducibility of the spectrum patterns was significantly hindered by changes in TMAH concentration and cell number, and stabilizer implication but not by growth temperature. Among those at low TMAH concentration(0.015m) was not observed the significant alterations of the spectrum pattern even when its injection volume was changed, yet was in different cell numbers and stabilizer implication.
김주현,Kim, Ju-Hyeon 대한흉부심장혈관외과학회 1979 The Korean Journal of Thoracic and Cardiovascular Vol.12 No.2
Eophagocardiomyotomy (modi/red Heller procedure) is a widely accepted operation for relief of dysphagia in patients with esophageal achalasia. But patients with advanced achalasia were more likely to get poorer results from a modified Ileller myotomy hecause of the dependent pouch that creates an angulation at the junction of thick-walled dilated esophagus with the thin walled aganglionic segment and hinders complete emptlllg. Thorhjarnarson(1975) prposcd the method including truncal vagectomy and pyloroplasty. Vagectomy and pyloroplasty should lesson the severity of acid-peptic esophagitis, if reflux should occur postoperatively. Here we presented 4 cases esophageal achalasia treated by modified Heller operation of 3 cases and one case of Thorbjarnarson method. All postoperative results are good.