Cellulose degrading basidiomycetes yeast isolated from the gut of grasshopper in Korea

김주영,장준휘,박지현,정희영,박종석,조성진,이훈복,성기호,김명겸,Kim, Ju-Young,Jang, Jun Hwee,Park, Ji-Hyun,Jung, Hee-Young,Park, Jong-Seok,Cho, Sung-Jin,Lee, Hoon Bok,Limtong, Savitree,Subramani, Gayathri,Sung, Gi-Ho,Kim, Myung The Microbiological Society of Korea 2018 미생물학회지 Vol.54 No.4

메뚜기는 광합성으로 고정된 탄소의 소화에서 중요한 역할을 한다. 장내 미생물 군의 도움으로, 메뚜기는 셀룰로오스 및 헤미셀룰로오스와 같은 잎의 성분을 분해할 수 있다. 본 연구는 한국의 기도에서 수집한 메뚜기 껍질에서 추출한 셀룰로오스 분해 효모 균주를 조사하기 위해 이루어졌다. 효모 균주 중 ON2와 ON17 (두 균주)과 ON6 (한 균주)는 CMC-플레이트 분석에서 셀룰로오스 활성을 보였다. Large subunit rDNA의 D1/D2영역의 서열과 internal transcribed spacer (ITS) 영역의 분석 결과, ON2와 ON17 균주가 Papiliotrema aspenensis CBS $13867^T$와 가장 밀접하게 관련되어 있었고(D1/D2 영역의 서열 유사성은 100% ITS에서 99.4%의 서열 유사성) ON6 균주는 Saitozyma flava와 관련된(D1/D2영역에서 100%, ITS에서 99.0%) 밀접하게 관련이 있었다. 이 세 가지 효모 균주는 모두 셀룰로오스를 분해할 수 있으므로 공생하는 효모들은 탄수화물 분해를 위한 효소를 자체적으로 생산하고 당 단당체를 휘발성 지방산으로 전환시킬 수 있다. Tremellomycetes에 속하는 공생 효모 균주인 ON2, ON17 (Papilioterma 속)과 ON6 (Saitozyma속)은 한국에는 보고되지 않은 균주이다. Grasshoppers play vital role in the digestion of photosynthetically fixed carbons. With the aid of intestinal microflora, the grasshopper can degrade leaves constituents such as cellulose and hemicellulose. The purpose of this study was to examine cellulolytic yeast isolates from the gut of grasshoppers collected in Gyeonggi Province, South Korea. Among the yeast isolates, ON2, ON17 (two strains), and ON6 (one strain) showed positive cellulolytic activity in the CMC-plate assay. The sequence analyses of D1/D2 domains of the large subunit rDNA gene and the internal transcribed spacer (ITS) regions revealed that the strains ON2 and ON17 were most closely related to Papiliotrema aspenensis CBS $13867^T$ (100%, sequence similarity in D1/D2 domains; 99.4% sequence similarity in ITS) and strain ON6 related to Saitozyma flava (100% in D1/D2 domains; 99.0% in ITS). All these three yeast strains are capable of degrading cellulose; therefore, the members of endosymbiotic yeasts may produce their own enzymes for carbohydrate degradation and convert mobilized sugar monomers to volatile fatty acids. Thus, the endosymbiotic yeast strains ON2, ON17 (represents the genus Papilioterma) and ON6 (Saitozyma) belonging to the family Tremellomycetes, are unreported strains in Korea.

만성 복통과 발진을 주소로 내원한 6세 여아에서 진단된 폐흡충증 1예

김주영,박민규,이용주,허선,조기영,Kim, Ju Young,Park, Min Kyu,Lee, Yong Ju,Huh, Sun,Cho, Ky Young 대한소아감염학회 2018 Pediatric Infection and Vaccine Vol.25 No.1

Pleural paragonimiasis is uncommon in the pediatric population and therefore can be challenging to diagnose. This is a case of a 6-year-old girl with pleural effusion who had been having intermittent persistent epigastric pain and erythematous rash on the face, hands, and arms for 6 months. Exudative pleural effusion with prominent eosinophils and serum eosinophilia were observed. As patient showed high immunoglobulin M (IgM) titers against Mycoplasma pneumoniae, she was treated with antibiotics; however, the pleural effusion did not improve during hospitalization. Despite showing negative stool ova and cyst results, patient's serum and pleural effusion were positive for Paragonimus westermani-specific IgGs on enzyme-linked immunosorbent assay. Respiratory symptoms, pleural effusion, and skin symptoms improved after praziquantel treatment.

Isolation and characterization of cellulolytic yeast belonging to Moesziomyces sp. from the gut of Grasshopper

김주영,정희영,박종석,조성진,이훈복,성기호,가야쓰리 수브라마니,김명겸,Kim, Ju-Young,Jung, Hee-Young,Park, Jong-Seok,Cho, Sung-Jin,Lee, Hoon Bok,Sung, Gi-Ho,Subramani, Gayathri,Kim, Myung Kyum The Microbiological Society of Korea 2019 미생물학회지 Vol.55 No.3

효모와 곤충 간의 집중적인 상호 작용은 곤충의 먹이에 대한 유인과 발달 및 행동에 대한 관련성을 보였다. 곤충 내장에서 분리된 효모는 먹이의 소화를 돕는 셀룰라아제(셀룰로오스 분해)를 분비한다. 한국의 경기도에서 수집한 메뚜기의 장에서 셀룰로오스를 분해하는 효모 세 균주를 분리 하였다. 효모 균주의 cellulase 활성을 확인하기 위해, 카르복시 메틸 셀룰로즈(CMC)를 함유하는 배지로 플레이트상의 투명한 영역을 요오드 용액을 사용하여 관찰하였다. 효모 $ON22^T$, $G10^T$ 및 $G15^T$ 균주는 CMC-플레이트 분석에서 양성 셀룰로오스 활성을 나타냈다. Large subunit rDNA 유전자와 Internal transcribed spacer (ITS) 영역의 D1/D2 영역의 서열 분석에 기초한 계통수를 통해 $ON22^T$와 $G10^T$ 균주가 Moesziomyces aphidis JCM 10318 (D1/D2 영역에서 각 100%와 99.8%, ITS 영역에서 각 98.4% 및 99.5% 서열유사성)와 가장 가깝고 G15는 Moesziomyces antarcticus JCM $10317^T$ 종 (D1/D2 영역에서 100%, ITS에서 100% 서열 유사성)에 속한다는 것을 밝혔다. 셀룰라아제는 바이오 에탄올 생산과 같은 바이오 연료와 같은 다양한 산업 공정에서 사용되고 있다. 따라서, 셀룰로오스 분해 미생물의 분리 및 연구는 중요성을 갖게 되었다. 이 논문은 한국의 Moesziomyces 속의 셀룰로오스 분해 효모 균주인 $ON22^T$, $G10^T$, $G15^T$에 대한 첫 번째 보고이다. An intensive interaction between yeasts and insects has highlighted their relevance for attraction to food and for the insect's development and behavior. Yeast associated in the gut of insects secretes cellulase which aided in the food digestion (cellulose degradation). Three strains of cellulose-degrading yeast were isolated from the gut of adult grasshoppers collected in Gyeonggi Province, South Korea. The strains $ON22^T$, $G10^T$, and $G15^T$, showed positive cellulolytic activity in the carboxymethyl cellulose (CMC)-plate assay. The phylogenetic tree based on sequence analysis of D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer (ITS) regions revealed that the strains $ON22^T$ (100 and 98.4% sequence similarities in D1/D2 domains and ITS) and $G10^T$ (99.8 and 99.5% in D1/D2 domain and ITS region) were most closely related to the species Moesziomyces aphidis JCM $10318^T$; $G15^T$ (100% in D1/D2 domains and ITS) belongs to the species Moesziomyces antarcticus JCM $10317^T$, respectively. Morphology and biochemical test results are provided in the species description. Cellulase with its massive applicability has been used in various industrial processes such as biofuels like bioethanol productions. Therefore, this is the first report of the cellulolytic yeast strains $ON22^T$, $G10^T$, and $G15^T$ related to the genus Moesziomyces in the family Ustilaginaceae (Ustilaginales), in Korea.

저강도 초음파 치료기기의 안전성 및 성능평가 가이드라인 수립을 위한 연구

김주영,김재영,노시철,최흥호,Kim, Ju-Young,Kim, Jae-Young,Noh, Si-Cheol,Choi, Heung-Ho 대한의용생체공학회 2015 의공학회지 Vol.36 No.5

In this study, we suggested the performance and safety testing guideline for low intensity pulsed ultrasound (LIPUS) represented by the ultrasound fracture treatment device and cartilage treatment device and low intensity focused ultrasound (LIFU) represented by ultrasonic face lifting device. For these study, the international standards and management regulations of Korea, Japan and United State were analyzed. And the usefulness and applicability were evaluated by testing with commercial equipment and reflecting the views of the industry and experts. As a result of this study, the safety and performance test guidelines for low intensity therapeutic ultrasound device were proposed by presenting the 10 items for LIPUS and 12 items for LIFU. The suggested guidelines are considered a high utilization in the domestic testing and approval authorities. And they are also thought to be useful to new technology development.

선박 항해통신장비 원격유지보수를 위한 데이터베이스 설계

김주영,옥경석,김주원,조익순,Kim, Ju-young,Ok, Kyeong-suk,Kim, Ju-won,Cho, Ik-soon 한국정보통신학회 2017 한국정보통신학회논문지 Vol.21 No.11

SOLAS 선박은 SFI group 코드 기준으로 적어도 83종의 장비를 탑재해야 하고, 각각의 장비는 다시 수 개에서 수십 개의 컴포넌트로 구성이 된다. 선박 운항 중에는 이러한 장비의 정상적인 운영이 보장되어야 하고, 문제 발생시 즉각적인 수리를 위하여 원격유지보수의 요구사항이 높다. 본 연구는 항해통신장비의 원격유지보수에 적합한 장비의 분류체계를 제시하고, 적절한 데이터베이스 구조를 도출하고자 했다. 연구 결과, 선박 항해통신장비의 원격유지보수를 위하여, 장비의 분류체계는 장비의 종류, 모델, 컴포넌트로 계층화되어야 하고, 고장모드와 영향분석, 질의응답, 수리이력, 상태정보와 예방정비 등의 테이블을 장비 모델 기준으로 구축하는 것이 효과적임을 알게 되었다. 140개의 항해통신장비 모델과 750개의 컴포넌트에 대하여 데이터베이스를 구축하였고, 실질적인 효과를 평가하기 위하여 선박장비 수리전문가가 클라우드 앱을 이용하여 검색 결과의 유용성을 평가하였다. The SOLAS ship should carry at least 83 different types of equipment based on the SFI group codes and each of which consists of several to dozens of components. During ship operation, it is necessary to ensure the normal operation of such equipment, and remote maintenance is highly demanded for immediate repair in the event of a equipment fault. This study proposes to find suitable classification system and to derive database structure for remote maintenance of navigation and communication equipment. As a result of this study, the classification system of equipment should be layered into equipment type, model, and component, and main table in the database consists of FMEA, service history, case data through Q&A, Preventive Maintenance. A database was constructed for 140 navigation and communication equipment models and 750 components. In order to evaluate the practical effects, service engineer evaluated the usefulness using the cloud app.

총대장균군 측정의 정도관리에 적합한 균주의 조제

김주영,서은영,김미리,전남희,정현미,김명운,안태석,Kim, Ju-Young,Seo, Eun-Young,Kim, Mi-Ree,Jeon, Nam-Hui,Chung, Hyen-Mi,Kim, Myeong-Woon,Ahn, Tea-Seok 한국미생물학회 2008 미생물학회지 Vol.44 No.1

정도관리는 분석 결과의 신뢰성을 높이기 위해 필수적인 과정이며, 이를 위해서는 일정한 농도의 표준시료가 필요하다. 본 연구에서는 정도관리의 정략분석을 위해 실온에서도 일정시간 개체수가 유지되는 미생물 정도관리용 표준시료를 제작하였다. 대장균으로 조제한 미생물 시료에 세포 분열을 억제하기 위해 nalidixic acid ($100\;{\mu}g/ml$)와 cephalexin ($50\;{\mu}g/ml$)을 첨가하였다. 이후 12시간 간격으로 acridine orange로 염색 후 현미경으로 계수하는 방법과 한천 배지에 배양하여 집락을 계수하는 평판집락계수법으로 개체수 변화를 측정하였다. 현미경 계수 결과 초기간이 $3.7{\times}10^5\;cells/ml$이었을 때 대장균의 개체수는 48시간 동안 $3.5{\sim}4.2{\times}10^5\;cells/ml$의 범위를 보였고, 평판집락법 결과 초기값이 $1.2{\times}10^4\;CFU/ml$ 이었을 경우에는 $1.0{\sim}1.2{\times}10^4\;CFU/ml$로 조사되어 냉장 및 냉동의 필요 없이 실온에서도 48시간 동안 개체수가 비교적 안정적으로 유지되는 미생물 표준시료를 제작할 수 있었다. 따라서 본 연구에서 제작된 표준시료는 현재 상업적으로 판매되고 있는 표준균주 제품과 함께 정도관리의 정략분석을 위해 사용될 수 있을 것이다. Standard sample for quality control of total coliform measurement was procured by addition of nalidixic acid and cephalexin as bacteriostatic agents to Escherichia coli cultured broth. After making the standard sample, the number of E. coli was measured by fluorescence microscopic count method and plate count method by 12 hr interval. The numbers of E. coli remained unchanged for at least for 48 hr at room temperature which ranged from 3.5 to $4.2{\times}10^5\;cells/ml$ and from 1.0 to $1.2{\times}10^4\;CFU/ml$ by direct fluorescence microscopic count method and plate count method, respectively. This result suggests that microbial standard sample with bacteriostatic agents of nalidixic acid and cephalexin is usable for quantitative quality control.

국내 건설 생산성 저해요인 분석 및 향상방안 제시

김주영,최재현,이승현,Kim, Ju-Young,Choi, Jae-Hyun,Lee, Seung-Hyun 대한건축학회 2011 대한건축학회논문집 Vol.27 No.2

A construction project is accomplished by the organized production with various and complicated activities. Productivity management for each activity is very important for completing the project successfully. In general, this work productivity is utilized as the index which shows the construction capacity of a construction company and affects to its profit ultimately. So, the improvement of work productivity through the effective field management is an important issue that should always be considered. In case of domestic construction industry, the terminology and concept of productivity seems to be very common, but its meaning is still regarded as ambiguous and inclusive. Specifically, most of researches relating to productivity are performed with an up-to-date keyword. However, the foundation studies such as the identification and analysis of productivity impact factors are actually insufficient. Therefore, this research identified and analyzed productivity loss factors which may occur on construction job sites. Also, it tried to suggest both the causes and solutions for productivity loss with the Delphi technique and reliability analysis. Based upon the research result, it is supposed to improve work productivity by acknowledging and making efforts to avoid potential risks associated with those identified factors early ahead of performing construction projects.

위상수학 강좌의 새로운 지도 방안

김주영,Kim, Ju-Young 영남수학회 2010 East Asian mathematical journal Vol.26 No.2

The main purpose of this work is to suggest a new approach to teaching general topology to undergraduate students. First, we change the order of contents. Usually, we introduce definitions, examples, theorems, and problems in this order. But, showing familiar examples of some concept of topology prior to the definition of one is the best method so far as I know from teaching experience of topology, which students feel very hard because of its abstractness. For more efficiency, mathematics-clubs each consisting of three or four students in topology course are established. This makes students very positive in topology class.

토끼눈에서 기구에 따른 각막상피제거술 후 손상 정도의 비교

김주영,이종혁,이석준,윤이나,Ju-young Kim,Jong-Hyuck Lee,Suk-Jun Lee,Ie-Na Yoon 대한안과학회 2010 대한안과학회지 Vol.51 No.9

Purpose: A corneal epithelial debridement using three different instruments was performed in rabbits, and the rates of corneal epithelium recovery were compared. Additionally, the extent of corneal damage as determined by the scanning electron microscopy was evaluated in each group. Methods: Nineteen eyes of ten rabbits were classified into three groups according to the instruments used. The corneal epithelial debridement was performed using three different instruments: a Beaver blade (group A), a Bard-Parker blade No.15 (group B) and a dry cotton–tipped applicator (group C). After epithelial debridement, each cornea was observed every 24 hours for three days. After completion of the corneal recovery, each cornea was severed along the limbus and observed under the scanning electron microscope. Results: The rate of corneal epithelial healing of the group C (dry cotton-tipped applicator) showed no statistical significance from those of the other groups. However, according to the corneal status observed under scanning electron microscope after debridement, cracks in the corneal surface in portions of group A and B were observed in contrast with no creaks in group C. Conclusions: Based on these results, corneal epithelial debridement using a cotton-tipped applicator is expected to reduce the occurrence of postoperative corneal complications. Use of a dry cotton-tipped applicator for corneal epithelial debridement in vitreoretinal surgery is suggested. J Korean Ophthalmol Soc 2010;51(9):1264-1270

Poly ( Styrene - co - GMA ) 의 합성과 in situ Reactive Compatabilizer 로서의 응용

김주영,서경도 ( Ju Young Kim,Kyung Do Suh ) 한국공업화학회 1992 공업화학 Vol.3 No.3

모노머의 반응성비가 각각 r₁=0.53, r₂=0.44반응인 styrene과 GMA(glycidyl methacrylate)를 일정 몰비로 반응시켜서 styrene과 GMA의 공중합체인 PGS를 합성한 후, 에틸렌 디아민을 반응시켜서 공중합체내에 아민기를 도입시켰다. 아민기가 도입된 중합체인 NPGS는 PGMA와 블렌드시에 DSC분석 결과, 단일 Tg를 나타내므로 두 폴리머는 상용성이 있다는 것이 관찰되었다. 이 NPGS-PGMA의 블렌드를 PS-PGMA블렌드물에 일정무게비로 첨가하여서 이에 따른 상용성의 변화를 관찰하였다. 즉, 비상용성인 PS-PGMA블렌드에, 에폭시기와 아민기와의 화학적 반응에 의하여 상용성을 나타내는 NPGS-PGMA블렌드의 첨가에 따른 상용성의 향상을 DSC분석을 통한 Tg변화와 SEM(Scanning electron microscopy)측정에 의한 morphology변화를 통하여서 관찰하였다. 측정 결과, PS-PGMA 블렌드는 NPGS-PGMA 블렌드가 첨가됨에 따라 Tg변화를 나타내었고, PGMA의 PS내에서의 분산은 향상되었다. 따라서, NPGS-PGMA의 블렌드는 PS-PGMA블렌드의 상용성을 향상시키는 상용화제로서의 작용을 하였다. Copolymer of Styrene and GMA(glycidyl methachytate), having reactive ratios of r₁=0.53, r₂= 0.44, was synthesized in dioxane using AIBN as free radical initiator. Followed by the reaction of ethylene diamine with copolymer PGS, amine groups were introduced to the PGS(NPGS). The composition of copolymer was determined by elementa] analyzer. Poly(glycidyl methacrylate) (PGHA) was obtained in benzene using AIBN as free radical initiator. The NPGS-PGMA blend of 50/50 composition was prepared by mixing these polymers in THF at 65℃. Glass transition temperature (Tg) of NPGS-PGMA blend was measured by DSC. The blend showed a single Tg. Accordingly, it was clear that the NPGS was compatible with PGMA. An intermolecular reaction between amine groups of NPGS and epoxy groups of PGMA imparts compatibility in the NPGS-PGMA blend. When the NPGS-PGMA blend was added to the incompatible PS-PGMA blend, PS-PGMA blend showed Tg change. Scanning Electron Micrograph(SEM) showed a fine morphology in this blend. Consequently, it was apparent that the NPGS-PGMA blend acted as a compatibilizer for the PS-PGMA blend.