Phenol - Chloroform - Petroleum ether 법을 이용한 Vibrio vulnificus lipopolysaccharide의 추출 및 생물학적 활성 측정

김주섭,박석돈 ( Ju Seob Kim,Seok Don Park ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.4

Background: Vibrio vulnificus is a pathogenic, marine, halophilic, gram-negative bacillus which causes fulminant infecticn in humans through the ingestion of raw seafood or skin wounds. V. vulnificus produces seveal kinds of virulent factors including cytolysin, endotoxin, exoenzymes, and siderphores. Among these, the lipopolysaccharide(LPS) of V. vulnificus has recently been purified, but the biological activity of this endotoxin is not well clarified as yet. Objective : The purpose of this study was to extract LPS from Vibrio vulnificus and to test the biological activity of extracted LPS for the elucidation of the role in V. vulnificas septicemia. Methods : V. vulnificus LPS was extracted by the Phenol Chloroform-Petroleurn ether(PCP) method. The biological activity of LPS was evaluated with a limulus amebocyte lysate assay and by assessment of lethality to ICR mice. Results : Five hundreds mg of LPS were extracted from 10g of dried V. vulnificus. Lirnulus amebocyte lysate formed a hard gel in response to the extracted LPS. This LPS showed low level of protein contaminatior in SDS-PAGE electophoresis and spectrophotometry. A High dose of LPS(200 mg/ml body weight) was lethal to mice. Conclusion : The PCP extraction yield relatively large amounts of LPS from V. vnlnificus. with out significant protein contamination and e xtrated LPS has endotoxin activity. This extrated LPS can be used for further studies such as making antibody or characterizing pathogenic roles in the V. vulnificus infection. (Kor J Dermatol 1997;35(4): 645-650)

할미꽃에 의해 발생한 자극피부염

김주섭,차승훈,박석돈 ( Ju Seob Kim,Seung Hoon Cha,Seok Don Park ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.2

Pulsatilla koreana is a member of the buttercup familly(Ranuculaceae) which contains the unsaturated lactone, protoanemonin(C3H4O2), formed after injury to the plant by the breakdown of the glycoside ranunculin. It has been prescribed as a herbal medicine or a folk medicine for antipyretic, analgesic, anti-inflammatory, astringent and hemostatic effects in Korea. A 20 year-old famale patient presented with confluent vesicobullae on the erythematous base with a burning sensation on her upper back. This was caused by contact with crushed Pulsatilla koreana, a folk medicine for the treatment of left facial nerve palsy, that had occurred 10 days before her visit. A provocation patch test with Pulsatilla koreana upon a heathy male volunteers upper back showed strong positive reactions with the stalk and leaf in fresh and frozen stat.e and after thaw ing resptively. However no reaction was seen when the folk medicine was used in a dried form or with the root in any state. This case indicates that misadvice and ignorant folk remedies could cause misfortunate results to innocent people, (Kor J Dermatol 1997;35(2): 339-343)

노동교육 : "노동자 학습재원" 마련이 시급하다

김주섭 ( Kim Ju Seob ) 한국노동사회연구소 2002 노동사회 Vol.66 No.-

조직문화와 긍정심리자본, 조직몰입의 인과관계에 대한 다수준분석

김주섭 ( Ju Seob Kim ),박재춘 ( Jae Choon Park ) 한국인적자원관리학회 2013 인적자원관리연구 Vol.20 No.3

본 연구는 조직수준에서의 조직문화 유형이 최근에 조명되고 있는 긍정적 조직행동인 긍정심리자본과 어떠한 상호작용을 하며, 조직문화와 조직몰입 간 블랙박스(black box), 즉 매개변수로서 긍정심리자본의 역할을 검증하고자, 비영리조직인 대학의 직원을 대상으로 위계적 선형모형(hierarchical linear model: HLM)을 활용하여 다수준(multi-level) 분석하였다. 연구결과 첫째, 긍정심리자본의 선행요인으로 조직문화 유형의 영향에 있어서는 조직문화 유형중 관계 문화와 합리문화가 개인수준의 긍정심리자본에 긍정적인 정(+)의 영향을 보였다. 그러나 혁신문화는 긍정 심리자본에 통계적으로 유의미한 부(-)의 영향을 미쳤다. 둘째, 조직문화 유형과 조직몰입의 관계에서는 관계문화와 위계문화가 조직몰입에 긍정적인 정(+)의 영향을 주었다. 셋째, 긍정심리자본과 조직몰입에 대한 결과는 긍정심리자본이 조직몰입에 긍정적인 정(+)의 영향을 주었다. 넷째, 긍정심리자본에 대한 매개효과에 대한 분석결과, 긍정심리자본은 관계문화와 조직몰입간 관계에서 부분매개역할을 확인하였다. 토의 및 결론을 통해 본 연구의 시사점과 한계점 및 향후 연구방향을 제시하였다. The purpose of this article examines the causal relationship among organizational culture, PsyCap, and organizational commitment(OC) using hierarchical linear model(HLM). The data were collected from total 597 employees of 31 university/college in KOREA. The main finding of the study were as follows: First, the main effect of clan culture and market culture was positively significant on PsyCap, but adhocracy culture was negatively significant. Second, the present study revealed that clan culture and hierarchy culture had a significant positive effects on OC. But the relationship between market culture(adhocracy culture) and OC did not turn out to be statistically significant. Third, PsyCap was positively related to OC. Finally, PsyCap acted a part mediator between clan culture and OC. But another culture types was no mediating effects between organizational culture type and OC. In conclusion, this article emphasizes the importance of positive psychological capital in facilitating further understanding of relationship between organization culture type and OC in university/college. Also this study is very meaningful because of overcoming the methodological limitations of existing research using HLM. In particular, this review concludes with recommendation for future, limitations of this study, and theoretical and managerial implications.

진로장벽의 하위변인과 진로스트레스의 관계에서 진로결정 자기효능감의 조절효과

김주섭(Ju Seob Kim),박재춘(Jae Chun Park) 학습자중심교과교육학회 2022 학습자중심교과교육연구 Vol.22 No.4

목적 본 연구는 대학생들의 진로스트레스를 감소시켜 효과적인 진로결정을 지원하고자 진로스트레스에 영향을 주는 변인을 규명하고자 하였다. 이를 위해, 진로장벽의 하위변인(직업정보 부족, 자기명확성 부족, 필요성 인식 부족, 우유부단한 성격)과 진로스트레스의 관계를 실증하고, 진로장벽과 진로스트레스의 관계에서 진로결정 자기효능감의 조절효과를 분석하였다. 방법 서울 및 충남권 소재 4년제 대학교의 대학생 314명을 대상으로 진로스트레스 관련 변인을 측정하기 위한 검증을 실시하였다. 종속변인으로서 진로스트레스, 독립변인으로 진로장벽, 조절변인으로 진로결정 자기효능감을 선정하였다. 변인들 간의 검증을 위해, 타당성 및 신뢰성 분석, 상관관계 분석을 하였으며, 연구문제 규명은 다중 회귀분석과 Cohen & Cohen(1983)의 조절효과 분석으로 검증하였다. 결과 실증분석 결과, 진로장벽의 하위변인 중 직업정보 부족과 자기명확성 부족 그리고 필요성 인식 부족 순으로 대학생들의 진로스트레스를 높이는 것으로 분석되었다. 그러나 진로장벽의 하위변인인 우유부단한 성격은 진로스트레스에 별다른 영향을 주지 않았다. 또한 우유부단한 성격을 제외한 진로장벽 하위변인 중 직업정보 부족과 자기명확성 부족 그리고 필요성 인식 부족과 진로스트레스의 관계에서 진로결정 자기효능감의 조절효과가 규명되었다. 결론 대학생들의 진로스트레스를 줄이기 위해서는 대학생들이 지각하는 진로장벽을 줄이기 위한 교육과 정책을 수반되어야 하겠다. 특히, 진로결정 자기효능감은 진로스트레스에 대한 진로장벽의 하위변인인 직업정보 부족과 자기명확성 부족 그리고 필요성 인식부족의 부정적 영향을 감소시켰다. 따라서 대학생들의 진로결정 자기효능감을 높이기 위한 체계적이고 다양한 교육이 필요하다 하겠다. Objectives The purpose of this study was to identify the factors affecting career stress in order to support effective career decisions by reducing career stress of Korean undergraduates. To this end, the relationship between the sub-variable of career barriers and career stress was demonstrated, and the moderating effect of career decision-making self-efficacy in the relationship between career barriers and career stress was analyzed Methods For empirical analysis, career stress-related variables were verified for 314 undergraduates students in Seoul and Chungnam. Career stress as dependent variables, career barrier as independent variables, and career decision-making self-efficacy as control variables were selected. Validity and reliability analysis, and correlation analysis were performed for verification between variables. The research hypothesis was verified by multiple regression analysis and Cohen & Cohen (1983) s moderating effect analysis. Results As a result of empirical analysis, it was analyzed that three subcomponents of career barriers(lack of job information, lack of self-clarity, and lack of awareness of necessity) increased career stress of undergraduates. However, the indecisive personality had no significant effect on career stress. Also, the moderating effect of career decision-making self-efficacy was investigated in the relationship between the three subcomponents of career barriers(lack of job information, lack of self-clarity, and lack of awareness of necessity) and career stress. Conclusions Education and policies to reduce career barriers and career stress of undergraduates should be accompanied. In particular, career decision-making self-efficacy reduced the negative effects of three subcomponents of career barriers(lack of job information, lack of self-clarity, and lack of awareness of necessity) on career stress. Therefore, it is necessary to provide systematic and diverse education to enhance the career decision-making self-efficacy of undergraduates.

대학생들의 취업불안과 취업준비행동의 관계에서 사회적 지지의 조절효과

김주섭(Kim Ju Seob) 학습자중심교과교육학회 2020 학습자중심교과교육연구 Vol.20 No.19

본 연구는 대학생들의 취업불안과 취업준비행동의 인과관계와 사회적 지지의 조절효과를 규명하였다. 본 연구는 충남 4년제 대학의 취업관련교육을 받는 학생 300명을 대상으로 설문을 실시하여 최종적으로 269명의 자료를 활용하여 실증하였다. 주요연구결과는 첫째, 취업불안과 취업준비행동의 관계를 검증한 결과, 대학생이 지각하는 취업불안은 취업준비행동에 긍정적인 정(+)의 영향을 주었다. 이는 대학생들이 취업불안을 높게 지각할수록, 취업준비행동이 증가된다는 것을 의미한다. 둘째, 사회적 지지의 조절효과 검증을 실시한 결과, 취업불안이 높은 학생들은 사회적 지지에 따라서 취업준비행동에 많은 차이를 보였다. 이는 취업불안을 느끼는 학생들에게 사회적 지지를 투여함에 있어, 사회적지지의 투여정도에 따라서 취업준비행동에 차이가 있음을 의미하는 것이다. 이러한 연구결과를 토대로 하여 본 연구의 시사점 그리고 연구한계와 향후 연구방향을 논하였다. This study identified the causal relationship of university students employment anxiety and employment preparation behavior and the moderating effect of social support. The study was conducted on 300 students who received employment-related education at a four-year university in Chungcheongnam-do, Korea, and finally, data from 269 were used. The main research results are as follows. First, as a result of examining the relationship between employment anxiety and employment preparation behavior, employment anxiety perceived by university students had a positive(+) effect on employment preparation behavior. This means that the higher the awareness of employment anxiety, the higher the employment preparation behavior of university students. Second, as a result of verifying the moderating effect of social support, students with high employment anxiety showed many differences in employment preparation behavior according to social support. This means that in giving social support to students who feel employment anxiety, there is a difference in employment preparation behavior depending on the degree of social support. Based on these, the implications of this study and future research directions were presented.

대한간학회지 제8차 춘계학술대회 초록집 : 포스터 전시 ; 원발성 간암과 감별이 어려웠던 부신선종 1예

김경호 ( Kim Gyeong Ho ),이자영 ( Lee Ja Yeong ),이진헌 ( Lee Jin Heon ),김용범 ( Kim Yong Beom ),김학양 ( Kim Hag Yang ),유재영 ( Yu Jae Yeong ),김주섭 ( Kim Ju Seob ),남은숙 ( Nam Eun Sug ) 대한간학회 2002 Clinical and Molecular Hepatology(대한간학회지) Vol.8 No.2(S)

인터넷 서비스를 위한 네트워크 진화

이상일(Sang Il Lee),김주섭(Ju Seob Kim) 한국정보과학회 1999 정보과학회지 Vol.17 No.3

Vibrio Vulnificus 세포용해소에 의한 마우스 독혈증시 Cytokine ( TNF - α , IL - β , IL - 6 ) 의 변화

박석돈 ( Seok Don Park ),김주섭 ( Ju Seob Kim ),차승훈 ( Seung Hoon Cha ) 대한피부과학회 1998 大韓皮膚科學會誌 Vol.36 No.1

Background: Cytokines such as TNF-a, and IL-1, 6, 8 are thought to mediate many host responses in patients with sepsis through the release of many kinds of inflammatory mediators. Vibrio vulnificus is a virulent, halophilic, marine, and Gram-negative bacterium causing wound infections or primary sepsis with fulminant course. It has been known to produce many exocellular factors, such as cytolysin and exozymes, which are possible causes of shock and tissue damage. Objective : Our purpose was to investigate the changes of cytokines(TNF-a, Il-l p, and IL-6) after intravenous administration of V. vulnificus cytolysin which have known to be a major virulent factor for elucidating pathogenesis of septic shock. Methods : After administration of V. vulnificus cytolysin(20 hemolytic units) and physiologic saline through a mouse tail vein, we obtained blood samples from the heart according to a time course(0, 30, 60, 90, 120, 240, and 360 minutes). We measured serum concentrations of circulating TNF- a, IL-1 p, and IL-6 using commercially available immunoassay kits. Results . The serum concentration of TNF- a peaked at 60 min (1894 pg/ml, as compared with 829 pg/ml after saline administration), began to decrease at 90 min and fell to baseline at 360min. Concentration of IL-6 rose significantly at 90 min(>3,839 pg/ml) and began to decrease at 120 min, whereas the level of IL-1 0 increased slightly and the peaked around 90min(117 pg/ml) after the cytolysin injection. Conclusion: We conclude that the host response to cytolysin is associated with a brief pulse of circulating cytokines in the early stage of sepsis caused by V. vulnipcus infection. (Korean J Dermatol 1998;36(1): 23-29)

진로불확실성이 진로준비행동에 미치는 영향

박재춘(Park, Jae Chun),김주섭(Kim, Ju-Seob) 학습자중심교과교육학회 2017 학습자중심교과교육연구 Vol.17 No.16

본 연구는 진로불확실성과 진로준비행동의 관계에서 진로결정 자기효능감의 매개 역할을 규명함과 동시에 진로준비행동에 대한 진로결정 자기효능감과 자기개념 명확성의 상호작용 효과를 검증하였다. 주로 활용한 연구방법은 기술통계, 상관분석 및 위계적 회귀분석이다. 실증연구 결과는 다음과 같다. 첫째, 대학생들이 지각하는 진로불확실성은 진로결정 자기효능감에 부정적인 영향을 주었다. 둘째, 진로불확실성은 진로준비행동에 부정적인 영향을 주었다. 셋째, 진로결정 자기효능감은 진로준비 행동에 긍정적인 영향을 주었다. 넷째, 진로불확실성과 진로준비행동의 관계에서 진로결정 자기효능감은 부분매개역할을 보였다. 끝으로 자기개념 명확성은 진로결정 자기효능감과 진로준비행동의 관계에서 조절역할을 보였다. 즉, 진로결정 자기효능감과 자기개념 명확성이 모두 높은 대학생들은 진로준비행동이 높았다. 그러나 자기개념 명확성이 높아도 진로결정 자기효능감이 낮은 대학생들의 진로준비행동은 매우 낮았다. 또한 토의 및 결론을 통해서 본 연구의 시사점과 한계점 및 향후 연구방향을 제시하였다. The study examines the influence of university students’ career uncertainty on career preparation behavior based on social cognitive career theory. In particular, this study investigates the mediating effect of career decision-making self-efficacy and the moderating effect of the self-concept clarity. The results of this study based on a sample of 308 university students was as follows. First, university students’ career uncertainty was negatively related to their career decision-making self-efficacy and career preparation behavior. Second, university students’ career decision-making self-efficacy was positively related to their career preparation behavior. Third, students’ career decision-making self-efficacy turned out to mediate the relationship between career uncertainty and career preparation behavior. Finally, students’ self-concept clarity moderated the relationship between career decision-making self-efficacy and career preparation behavior. In addition, this paper conclude with implications for future research, limitations of this study, and practical application.