Quantitative Analysis of Kynurenic Acid in Chestnut Honey from Different Regions and Method Validation

김주리,김도윤,이상현,Kim, Juree,Kim, Doyun,Lee, Sanghyun The Korean Society of Pharmacognosy 2022 생약학회지 Vol.53 No.2

Chestnut honey is a sweet dark-colored honey with a distinct bitter aftertaste. It contains numerous phenolic compounds and alkaloids and is noted for its antioxidant and anti-inflammatory activities. However, it has been established that there are differences in the composition and activity of chestnut honey constituents depending on the region of origin, the sources of which warrant further research. In this study, we analyzed the kynurenic acid (KA) contents in chestnut honey produced in nine different regions in Korea, using high-performance liquid chromatography in conjunction with ultraviolet detection, and validated the analytical method developed. Use of a reverse-phase column and detection at a wavelength of 240 nm were found to be optimal for the detection of KA. Similar evaluation of an optimal method for extracting KA from chestnut honey revealed that extraction using 10% EtOH at 20 times the sample volume over a 6 h period was the most suitable for obtaining a high content of KA. Among the nine regional chestnut honeys assessed, KA content was found to be highest in the "Gongju" sample (1.14 mg/g), followed by that in the "Cheongdo" and "Damyang" samples. Validation of the KA analytical method revealed a good analyte linearity, with a correlation coefficient (r²) of 0.9995, an accuracy of between 92.37% and 107.35%, and good precision (RSD ≤ 1.05%). Our findings in this study, based on a validated quantitative analytical method for KA, could make an important contribution to establishing a data profiling procedure for characterizing chestnut honeys produced in different regions, and may also provide basic data for the identification of functional honey.

유방암의 접선 조사시 피폭 폐용적

오영택(Young Taek Oh),김주리(Juree Kim),강해진(Haejin Kang),손정혜(Jeong Hye Sohn),강승희(Seung Hee Kang),전미선(Mison Chun) 대한방사선종양학회 1997 Radiation Oncology Journal Vol.15 No.2

목 적 : 유방암의 방사선 치료에서 가장 유의하여 할 사항 중의 하나인 방사선 폐렴 등의 폐합병증은 피폭 폐용적, 방사선량율, 방사선량 등의 영향을 받으며 그 중에서도 피폭 폐용적의 정도가 중요한 것으로 알려져 있다. 그러나 피폭 폐용적의 정량적인 측정 자료는 매우 드물며 피폭 폐용적의 정도를 예측할 수 있는 방법에 대한 보고도 제한적일 뿐만 아니라 대부분이 서양인을 대상으로 한 보고이다. 이에 본 저자들은 본원에서 치료받은 유방암 환자를 대상으로 접선조사시의 피폭 폐용적을 정량적으로 측정하고 간접적으로 피폭 폐용적을 예측할 수 있다고 제시되고 있는 여러 인자들의 유용성을 검증하고자 본 연구를 계획하였다. 재료 및 방법 :본 원 에 서 1995년 1월 부 터 1996년 8월 까지 접선 조사방식으로 방사선 치료를 시행 받은 유방암환자 중 치료 계획용 컴퓨터 단층 촬영을 시행한 25명을 대상으로 폐용적을 측정하였다 . 각각의 환자에서 피폭 폐용적을 예 측 할 수 있는 인자로서 1) 치료 계획 필름의 조사면 중심에서의 후방 접선으로부터 전방 흉벽의 뒷면까지의 수직선상거리인 Central Lung Distance (CLD), 2) 치료계획 필름의 후방 접선으로부터 전방 흉벽의 뒷면까지의 수직선중 가장 긴 수직선의 거리인 Maximum Lung Distance(MLD), 3) 치료 계획용 컴퓨터 단층 촬영 필름의 조사면 중심사진에서의 후방 접선으로부터 전방 흉벽의 뒷면까지의 거리인 Greatest Perpendicular Distance (GPD), 4) 치료계획 필름 상의 조사면의 세로길이(L)를 측정하였고 피폭 폐용적을 구하기 위하여 치료 계획상의 Dose Volume Histogram(DVH) 자료에서 양측 전체 폐용적(EV), 동측 전체 폐용적(IV) 및 피폭 폐용적(RV)을 측정하고, 서로간의 상관관계를 분석하였다. 결 과 : 총 25명 대상 환자의 연령은 23-67세로(중앙 연령 41세) 우측 유방암이 14예, 좌측 유방암이 11예 있었다. 전체 환자의 CLD는 평균 2.2cm(1.9-3.3cm), MLD는 평균 2.4cm(1.9-3.3 cm) 그리고 GPD는 평균 2.3cm(1.4-3.1cm)이었으며 L은 16-23cm이었다. CLD 와 L을 곱한 값은 평균 42.4cm2(32-76cm2), MLD와 L의 곱은 평균 45.3cm2(34.2 -75.9cm2)였으며, GPD와 L을 곱한 값은 42.5cm2(26.6-69cm2)였다. 전체 폐용적은 1356-4092cc로 평균 3052cc였으며 우측 폐는 584-2554cc 평균 1671cc였고 좌측 폐는 632-2252 cc로 평균 1379cc였다. 피폭 폐용적은 61-279cc(평균 170cc)로 양측 전체 폐용적에서 차지하는 비율은 (RV/EV)은 2.9-13%(평균 5.8%) 이고 동측 폐용적에서 차지하는 비율은(RV/IV) 4.9-29.6(평균 12.2%)였다. CLD, MLD, GPD, L, CLD*L, MLD*L, GPD*L 등의 변화에 따른 RV, RV/EV, RV/IV 등의 피폭 폐용적의 변화는 통계적으로 유의한 상관관계를 구할 수 없었으며 CLD가 3cm 이하인 24명의 환자에서 RV/EV는 10% 이내였다. 좌우 폐의 비교에서 RV/IV 이 좌측 유방암 환자에서 유의하게 높았으나 RV/EV은 유의한 차이를 나타내지 못하였다. 결 론 : 현재 사용하고 있는 접선 조사방식에서 CLD를 3cm 이내로 제한하는 경우 CLD 등의 변화폭이 작아서 피폭 폐용적과의 상관관계를 구할 수는 없으나 피폭 폐용적의 정도는 다른보고들을 고려할때 적절하였고 좌측 유방암 환자에서의 피폭 폐용적이 동측 전체 폐용적에서 차지하는 비율은 우측 유방암 환자에 비해 높았으나 양측 전체 폐용적에 대한 비율은 동일하였다. Purpose : Radiation pneumonitis is one of the complications caused by radiation therapy that includes a portion of the lung tissue. The severity of radiation induced pulmonary dysfunction depends on the irradiated lung volume, total dose, dose rate and underlying pulmonary function. It also depends on whether chemotherapy is done or not. The irradiated lung volume is the most important factor to predict the pulmonary dysfunction in breast cancer patients following radiation therapy. There are some data that show the irradiated lung volume measured from CT scans as a part of treatment planning with the tangential beams. But such data have not been reported in Korea. We planned to evaluate the irradiated lung volume quantitatively using CT scans for the breast tangential field and search for useful factors that could predict the irradiated lung volume. Materials and Methods : The lung volume was measured for 25 patients with breast cancer irradiated with tangential field from Jan.1995 to Aug.1996. Parameters that can predict the irradiated lung volume included; (1) the perpendicular distance from the posterior tangential edge to the posterior part of the anterior chest wall at the center of the field (CLD); (2) the maximum perpendicular distance from the posterior tangential field edge to the posterior part of the anterior chest wall (MLD); (3) the greatest perpendicular distance from the posterior tangential edge to the posterior part of anterior chest wall on CT image at the center of the longitudinal field (GPD); (4) the length of the longitudinal field (L). The irradiated lung volume(RV), the entire both lung volume(EV) and the ipsilateral lung volume(IV) were measured using dose volume histogram. The relationship between the irradiated lung volume and predictors was evaluated by regression analysis. Results : The RV is 61-279cc (mean 170cc), the RV/EV is 2.9-13.0% (mean 5.8%) and the RV/IV is 4.9-29.6% (mean 12.2%). The CLD, the MLD and the GPD are 1.9-3.3cm, 1.9-3.3cm and 1.4-3.1cm respectively. The significant relations between the irradiated lung volume such as RV, RV/EV, RV/IV and parameters such as CLD, MLD, GPD, L, CLD×L, MLD×L and GPD×L are not found with little variances in parameters. The RV/IV of the left breast irradiation is significantly larger than that of the right but the RV/EV s do not show the differences. There is no symptomatic radiation pneumonitis at least during 6 months follow up. Conclusion : The significant relationship between the irradiated lung volume and predictors is not found with little variation on parameters. The irradiated lung volume in the tangential field is less than 10% of entire lung volume when CLD is less than 3cm. The RV/IV of the left tangential field is larger than that of the right but there was no significant differences in RV/EVs. Symptomatic radiation pneumonitis has not occurred during minimum 6 months follow up.

재발성 자궁경부 편평상피암 환자들에서 Squamous Cell Carcinoma 항원의 유용성

최영민(Young Min Choi),박성광(Sung Kwang Park),조흥래(Heung Lae Cho),이경복(Kyoung Bok Lee),김기태(Ki Tae Kim),김주리(Juree Kim),손승창(Seung Chang Sohn) 대한방사선종양학회 2002 Radiation Oncology Journal Vol.20 No.4

목적 : 자궁경부 편평상피암의 치료 후 추적조사 시에 시행하는 혈중 Squamous Cell Carcinoma (SCC) 항원 검사가 재발의 조기 발견에 도움이 되는지 알아보고자 하였다. 대상 및 방법 : 1997년 1월부터 1998년 12월까지 자궁경부 편평상피암으로 방사선치료를 받고 완전 관해 되었다가 재발된 환자들 중에서 재발 시에 SCC 항원 검사가 이루어졌던 20명을 대상으로 하였다. SCC 항원은 환자의 혈칭으로부터 면역효소법으로 측정되었고, 2 ng/mL 미만을 정상으로 하였다. 진단, 추적조사, 재발 시의 SCC 항원값을 조사하여, SCC 항원 검사의 재발에 대한 민감도, 재발 양상에 따른 차이, 재발에 대한 증가 시기, 종양의 크기 및 병기와의 관계 등을 조사하였다. 결과 : 재발된 환자 20명 중의 17명에서 SCC 항원이 정상보다 증가되어 SCC 항원의 민감도는 85%였고, 평균은 15.2 ng/mL (중앙값: 9.5 ng/mL)였다. 재발 부위별로는 국소 재발, 원격전이, 동시에 발생한 경우 등으로 나누어서 SCC 항원을 비교하였으나 차이가 없었다. 재발을 진단하기 전 6개월 내에 SCC 항원이 검사되었던 11명에서 재발 전 SCC 항원은 평균이 13.6 ng/mL (중앙값: 3.6 ng/mL)였고, 7명에서 정상 보다 높았으며, 정상 범위였던 4명 중의 3명에서 1.5 ng/mL 이상으로 증가되는 양상이었다. 재발된 환자들 중에서 진단 시에 SCC 항원이 검사되었던 16명의 환자들에서 병기 lb, lla이면서 4cm 미만이었던 환자들에 비하여 병기 lb, lla이면서 4cm 이상이거나 병기 llb, lll이었던 환자들에서 진단 당시의 SCC 항원값이 높았다. 결론 : 자궁경부 편평상피암 환자들에서 추적조사 SCC 항원 검사는 재발의 조기 발견에 유용하고, 향후 효과적인 구제치료법이 개발되면 임상적인 이득이 더욱 증가될 것으로 생각된다. Purpose : To investigate the clinical usefulness of a follow-up examination using serum squamous cell carcinoma antigen (SCC) for the early detection of recurrence in patients treated for cervical squamous cell carcinoma. Materials and Methods : 20 patients who were treated for recurrent cervical squamous cell carcinoma between 1997 and 1998, who had experienced a complete remission after radiotherapy and who under-went an SCC test around the time when recurrence was detected, were included in this study. The levels of SCC were measured from the serum of the patients by immunoassay and values less that 2 ng/mL were regarded as normal. The sensitivity of the SCC test for use in the detection of recurrence, the asso-ciation between the SCC values and the recurrence patterns and the tumor size and stage, and the temporal relation between the SCC increment and recurrence detection were evaluated. Results : The SCC values were above normal in 17 out of 20 patients, so the sensitivity of the SCC test for the detection of recurrence was 85%, and the mean and median of the SCC values were 15.2 and 9.5 ng/mL, respectively. No differences were observed in the SCC values according to the recurrence sites. For 11 patients, the SCC values were measured over a period of 6 months before recurrence was detected, and the mean and median values were 13.6 and 3.6 ng/mL, respectively. The SCC values of 7 patients were higher than the normal range, and the SCC value of the other 4 patients were normal but 3 among them were above 1.5 ng/mL. At the time of diagnosis, the SCC valuess were measured for 16 of the 20 recurrent patients, and the SCC values of the patients with a bulky tumor (≥ 4cm) or who were in stage lib or lll were higher than those of the patients with a non-bulky tumor or who were in stage lb or lla. Conclusion : The SCC test is thought to be useful for the early detection of recurrence during the follow up period in patients treated for cervical squamous cell carcinoma. When an effective salvage treatment is developed in the future, the benefit of this follow-up SCC test will be increased.

절제가능한 직장암에서 실시한 샌드위치 테크닉의 치료결과

김주리,손승창 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.3

Objective : The aim of this study was to determine the survival outcomes and the morbidity of sandwich technique for the advanced resectable rectal cancer. Methods and Materials: Between 1991 and 1994, total 29 patients who had the locally advanced rectal cancer were treated with low dose preoperative radiotherapy, radical surgery, and then, postoperative radiotherapy with or without chemotherapy at the Inje University Paik Hospital. Eighteen of 29 patients were evaluated. The preoperative radiotherapy dose was 1500 cGy/ 5 fractions. The radical surgery was performed immediately after preoperative radiotherapy. The fluorouracil-based chemotherapy was delivered in 10 patients postoperatively. Results : The 5 year overall survival rate, relapse free survival. and local control rate were 52.3%, 48.0%, and 77.9%, respectively. The incidence of wound problem was 27.8%(5/18 patients). The severe side effect above grade 3 during preoperative and postoperative adjuvant therapy was none. The chronic side effects including adhesion or fibrosis of bowel were occurred in 5 patients. Three of these 5 patients were successfully managed by surgery. The preservation of anal function was good in six patients among nine patients with low anterior resection. Conclusion : Sandwich technique can be safely delivered in locally advanced rectal cancer. For acquiring the further T-level downstaging and treatment response, the preoperative radiotherapy dose may be increased.

항문암에서 항문보존을 위한 치료 결과

김주리,최영민,조홍래,손승창 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.1

■ Objectives Nonsurgical treatment of anal cancer by radiotherapy and chemotherapy is the standard treatment for primary epidermoid anal cancer. The aim of this study is to evaluate these results. ■ Methods and materials We analyzed 15 patients with epidermoid cancer of the anus who were treated by radiation therapy combined with chemotherapy at the Paik hospital from 1991 to 2000, retrospectively. ■ Results The local control rate was 77.3%. The overall 2-year and 5-year survival rates were 75.2% and 47.0%. respectively, The rate of anal preservation was 100%. ■ Conclusions According to these results and recent randomized trials, radical radiotherapy and chemotherapy can be recommended as the treatment of primary epidermoid anal cancer. Preservation of the anal function was good. But, for the better result, we should consider salvage surgery for the patients with partial response after chemoradiotherapy or recurrent tumor.

기저세포 및 편평상피세포 피부암에 대한 방사선 치료

전미선,김주리,이은소,강원형 아주대학교 의과학연구소 1996 아주의학 Vol.1 No.1

Skin cancers other than melanoma are highly curative disease because they are localized. In the last 14 months period, 11 patients with skin cancers(7 basal cell carcinomas, 3 squamous cell carcinomas, 1 metastasis from the lung cancer) were referred to the radiation oncology department for curative purpose in 9 patients and palliative purpose in 2 patients. Among the curative purpose patients, six patients had after one or few recurrences previously. Except 1 patient who had unusual multiple basal cell carcinomas sparing facial part, all other lesions were located in the face. Masses were a few mm to 5x6 cm in diameter. Since 3 patients had lesion near an eye, a lead eye shield was used for the prevention of cataract and keratitis/conjunctivitis. Also for lesions near or at the nose, a shield to protect nasal mucosa was used. Among patients who completed radiotherapy, all lesions disappeared except residual pigmentation which left some elevated mass effect. Careful irradiation technique and dose schedules were described with a follow-up time of I month to 14 months. In the future, cosmetic care for residual pigmentation needs to be followed.