랫드 일차 배양 간세포에서 에탄올의 독성에 대한 헛개나무 물추출물의 보호효과

김종호,서영민,김주현,현선희,이상규,김춘화,강미정,전태원,윤수홍,정태천,Kim, Jong-Ho,Seo, Young-Min,Kim, Ju-Hyun,Hyun, Sun-Hee,Lee, Sang-Kyu,Kim, Chun-Hwa,Kang, Mi-Jeong,Jeon, Tae-Won,Yoon, Soo-Hong,Jeong, Tae-Cheon 대한약학회 2008 약학회지 Vol.52 No.1

The hepatoprotective effects of the water extracts of Hovenia dulcis Thunb (HD) were investigated in vitro. Following the induction of hepatotoxicity by ethanol in primary cultures of rat hepatocytes, the protective effects of four different water extracts of HD were determined through serial dose-response and time-dependent studies. The individual extracts used in these studies were prepared from fruits, seeds, leaves and tubes. Treatment of hepatocyte cultures with the water extracts of HD provided a significant protection from the increased lactate dehydrogenase activity induced by ethanol. Particularly, the fruits extract was the most effective against ethanol-induced hepatotoxicity in the primary cultures of rat hepatocytes. The results demonstrated that the extracts might have the protective effect against ethanol-induced toxicity in hepatocyte cultures.

섬유성 골 이형성증의 골 신티그라피 소견

김종호,김종순,한승수,김상은,최창운,이동수,정준기,이명철,고창순,강흥식 ( Jong Ho Kim,Jong Soon Kim,Seung Soo Han,Sang Eun Kim,Chang Woon Choi,Dong Soo Lee,June Key Chung,Myung Chul Lee,Chang Soon Koh,Heung Sik Kang ) 대한핵의학회 1991 핵의학 분자영상 Vol.25 No.2

ARM920T 기반의 다기능 UHF RFID 리더 플랫폼 기술 개발

김종호,이상신,김영길,김용득,Kim Jong-ho,Lee Sang-sin,Kim Young-kil,Kim Yong-deak 대한전자공학회 2005 電子工學會論文誌-SC (System and control) Vol.42 No.2

본 논문은 RFID 태그 정보를 무선으로 수신하여 처리한 후 이들 정보를 RS-232, USB, Bluetooth 또는 Ethernet 인터페이스를 통하여 PC서버에 전달하는 기능을 수행하는 다기능 UHF 대역 RFID 리더 플랫폼의 설계 및 구현을 위한 것이다. 개발하고자 하는 Embedded RFID 리더는 크게 RFID 모듈, ARM 프로세서, RS-B2 인터페이스, USB 인터페이스, Enternet MAC 인터페이스, Bluetooth 인터페이스 등으로 구성된다. 본고를 통하여 RFID의 개념 및 리더의 동작원리를 소개하고 임베디드 프로세스를 사용한 리더플랫폼 및 관련 알고리즘을 제안하고 구현한다. 설계된 플랫폼에 대하여 고정형과 이동형으로 나누어 그 기능을 분석하고 평가한다. This paper is about designing and implementation of Multi-Function UHF RFID Reader Platform that receives and processes RFID tag data from the tags, and transmits the data to PC server through Bluetooth, USB, RS-232 or Ethernet. This reader system is composed of RFID module, ARM processor, RS-232 interface, USB, Ethernet MAC interface, Bluetooth etc.. This paper also provides a brief overview and concept of RFID reader, and proposes the design and implementations of the RFID reader platform. The paper evaluates and analyzes two practical implementations, fixed type and portable type.

NaI ( T1 ) 섬광결정과 위치민감형 광전자증배관을 이용한 유방암 진단용 소형 감마카메라 개발

김종호,최용,권홍성,김희중,김상은,최연성,이경한,김문회,주관식,김병태 ( Jong Ho Kim,Yong Choi,Hong Seong Kwon,Hee Joung Kim,Sang Eun Kim,Yearn Seong Choe,Kyung Han Lee,Moon Hae Kim,Koan Sik Joo,Byung Tae Kim ) 대한핵의학회 1998 핵의학 분자영상 Vol.32 No.4

Purpose: The conventional gamma camera is not ideal for scintimammography because of its large detector size ( 500 mm in width) causing high cost and low image quality. We are developing a small gamma camera dedicated for breast imaging. Materils and Methods: The small gamma camera system consists of a NaI (Tl) crystal (60 mm X60 mm X6 mm) coupled with a Hamamatsu R3941 Position Sensitive Photomultiplier Tube (PSPMT), a resister chain circuit, preamplifiers, nuclear instrument modules, an analog to digital converter and a personal computer for control and display. The PSPMT was read out using a standard resistive charge division which multiplexes the 34 cross wire anode channels into 4 signals (X, X, Y, Y ). Those signals were individually amplified by four preamplifers and then, shaped and amplified by amplifiers. The signals were discriminated and digitized via triggering signal and used to localize the position of an event by applying the Anger logic. Results: The intrinsic sensitivity of the system was approximately 8,000 counts/.'ec/pCi. High quality flood and hole mask images were obtained. Breast phantom containing 2-7 mm diameter spheres was successfully imaged with a parallel hole collimator. The image displayed accurate size and activity distribution over the imaging field of view. Conclusion: We have succesfully developed a small gamma camera using NaI(T1)-PSPMT and nuclear instrument modules. The small gamma camera developed in this study might imprnve the diagnostic accuracy of scintimammography by optimally imaging the breast. (Korean J Nucl Med 1998;32:365-73)

전혈 , 림프구와 백혈구 연층 각각의 방사성 동위원소 체외 조사후 배양을 이용한 생물학적 선량측정

김종호,이동수,최창운,정준기,이명철,고창순,김종순,김희근,강덕원,송명재 ( Jong Ho Kim,Dong Soo Lee,Chang Woon Choi,June Key Chung,Myung Chul Lee,Chang Soon Koh,Chong Soon Kim,Hee Geun Kim,Duck Won Kang,Myung Jae Song ) 대한핵의학회 1995 핵의학 분자영상 Vol.29 No.1

The purpose of this study was to establish mononuclear cell cultures such as lymphocytes or buffy coat for the biological dosimetry of in vitro irradiation of the radionuclide Tc 99m in order to exclude the effect of residual doses seen in the cultures of whole blood. Biological dosimetry of Tc-99m on cultured mononuclear cells at doses ranging from 0.05 to 6.00 Gy, by scoring unstable chromosomal aberrations(Ydr) observed in cultured lymphocytes, were performed using peripheral venous blood of healthy normal person. The results showed that; (1) Jn vitro irradiation of radioisotope in separated lymphocyte or buffy coat showed trace amount af residual doses of isotope after washing. Residual doses of isotopes are increased in proportion tn exposed time and irradiated dose without difference between I-131 anct Tc 99m. (2) We obtained these linear-quadratic dose response equations in lymphocyte and buffy coat culture after in vitro irradiation of Tc-99m, respectively (Ydr = 0,001949 D+ 0,006279B+ 0.000185; Ydr= 0.002531 D 0.003274 D+0.003488). In aonclusion, the linear quadrstic dose response equation from in vitro irradiation of Tc 99m with lymphocyte and buffy coat culture was thought to be useful for assessing Tc 99m indueed biological effects. And mononuclear cell cultures seem to be the most appropriate experimental rnodel for the assessment of biological dosimetry of internal irradiation of radionuclides.

경기지역 소아에서 발생된 삼일열 말라리아의 역학 및 임상적 특성

김종호,이윤경,김종현,허재균,장기영,강혜련,강진한,Kim, Jong Ho,Lee, Yoon Kung,Kim, Jong Hyun,Hur, Je Kyun,Chang, Ki Young,Kang, Hye Rhyun,Kang, Jin Han 대한소아감염학회 2000 Pediatric Infection and Vaccine Vol.7 No.2

목 적 : 1970년대 이후 약 20년간 발생이 없었던 삼일열 말라리아가 1993년부터 발생되어 점차 확산되는 추세에 있어 과거와 같은 토착화에 대한 우려가 높다. 국내에서 말라리아의 토착화를 막기 위하여는 현재 발생되는 말라리아 대한 역학 및 임상 특성에 관한 연구가 제일 우선되어야 할 것이다. 한편 성인에 비해 발생율은 낮으나 소아에서도 환자가 증가되는 추세에 있어 소아에서도 동일한 관점에서 역학 및 임상 연구가 요구되고 있다. 이와 같은 배경에서 말라리아 다발생 지역인 경기지역의 소아에 발생된 말라리아의 역학 연구를 후향적으로 실시하였다. 방 법 : 1998년 9월부터 1999년 8월까지 경기지역 종합병원 및 국공립 의료기관에서 말초 혈액도말 검사상 말라리아로 진단된 15세 이하의 소아 환자를 대상으로 발생 연령 및 성별, 발생 시기, 발생 지역, 발생 역학적 배경, 발열의 양상, 동반 증상, 진찰상 특이 소견, 일반검사 소견, 말초혈액 도말검사 소견, 치료와 연관된 사항 및 예후에 관한 내용의 의무기록을 통하여 후향적 연구를 시행하였다. 결 과 : 연구대상수는 총 21명으로서 남녀비는 2:1이었고 5세 이하에서도 삼일열 말라리아가 발생되었으나 11세 이상 15세 이하의 연령에서제일 많이 발생(14명;66.7%)되었다. 발생시기는 2월, 5,월, 6월, 10월에 각 1명씩 발생하였고 4월, 9월에 각 2명이 그리고 8월에 4명, 7월에 9명이 발생되었다. 환아가 발생된 동일 거주지역과 가족내에서 성인 환자 발생이 있었고, 지역적으로 파주, 의정부, 부평, 연천, 동두천, 포천, 철원, 부천의 순으로 다발생하였다. 임상적으로 발열의 양상은 48시간 간격의 주기성 발열이 15명, 24시간 간격의 주기성 발열이 4명, 비주기성 발열이 2명에서 보였고 발열외에 두통, 구토, 식욕부진, 오심, 오한, 권태감, 복통, 설사 등의 증상이 동반되었다. 진찰 소견상 비장비대가 제일 흔히 발현되었고, 검사 소견상 빈혈, 혈소판 감소증, 비정상 간기능, 백혈구 감소, 혈뇨의 순으로 이상 소견이 나타났다. 말초혈액 도말검사에서 Plasmodium vivax의 원충 단계는 young trophozite, gametocyte, schizont순으로 확인되었다. 전 예에서 chlorquine 약제의 치료반응이 좋았고 합병증이 없었으며 추적 가능한 8례에서도 재발은 없었다. 결 론 : 1998년 9월부터 1999년 8월 사이에 경기지역 소아에서 발생된 말라리아는 경기 북부지역의 10세 이상 고연령 소아에서 주로 하계절에 발생되며, 주기성 발열이 임상적으로 주된 증상이며 진찰상 비장비대가 주된 양성 소견이었으며 말초 혈액 도말검사상 young trphozoite가 제일 많이 발견됨을 알 수 있었다. 전반적으로 소아 환자에서의 치료 약제 반응은 좋고 합병증이 없이 양호한 경과를 보이며 재발도 거의 없는 것을 확인하였다. Purpose : In Korea, vivax malaria has been reemerged since 1993 after being abscent for more than 10 years. There are several possibilities of casuality of recent epidemic, although it is still unclear. The epidemiologic studies including case analysis and entomological reseach have been undertaken for a successful control measure. But, unfortunately those studies have been rarely dealt with cases of children. Therefore, this study was designed to figure out the characteristics of epidemiolgic and clinical features in children with indigenous vivax malaria. Methods : The study 21 cases below 15 years of age, who were diagnosed as vivax malaria and resided in kyounggi-do province area during 1998. 9~1999. 8. We retrospectively analyzed epidemiologic data concernig with occurrence of vivax, and clinical manifestations, abnormal laboratory findings and outcomes including therapeutic responses. Results : All cases were inhabitants of the endemic areas for vivax malaria in northwestern part of Kyonggi-do or western Kangwon-do, and Paju-gun was the most prevalent. Indigenous malaria cases of this study were more prevalent in children above 10 years old age, and in male. Seasonally, vivax malaria in children occurred throughout the year except January, March and November, and the incidence was the highest in July. Clinical manifestations revealed that 48 hour cyclic fever pattern was the major fever pattern, and other symptoms such as headache, vomiting, poor appetites, chilling, abdominal pain and diarrhea were concomitantly developed. And splenomegaly revealed the main abnormal findings on physical examination, and anemia was the most frequent abnormal finding in laboratory examinations. Young trophozoite was frequently observed on peripheral blood smears. The therapeutic responses of chlorquine were very good in all cases, and no recurrence developed in follow up cases. Conclusion : Geographical and seasonal occurrence distributions of indigenous vivax malaria cases in children were very similar to those of adults as followings; Inhabitants of the endemic region, more prevalent in male, and more common during the summer season. Clinically, 48 hour cyclic fever pattern, splenomegaly and anemia were most frequent and important manifestations in children cases, and clinical courses were not serious. On blood smears, young trophozoite was most dominantly examined in children. Generally, the therapeutic outcomes were excellent, and recurrences were not observed.

소형 감마카메라 제작에 사용되는 NaI(TI)- 광전자증배관 검출기의 민감도와 위치 분해능 특성 연구를 위한 몬테카를로 시뮬레이션

김종호,최용,김준영,임기천,김상은,최연성,주관식,김영진,김병태,Kim, Jong-Ho,Choi, Yong,Kim, Jun-Young,Im, Ki-Chun,Kim, Sang-Eun,Choi, Yeon-Sung,Joo, Kwan-Sik,Kim, Young-Jin,Kim, Byung-Tae 한국의학물리학회 1997 의학물리 Vol.8 No.2

본 연구에서는 유방암 진단용 감마카메라 제작 시에 고려되어야 하는 섬광체의 크기와 섬광체 면의 적절한 표면처리가 카메라의 민감도와 위치 분해능에 미치는 영향을 연구하기 위하여 섬광의 광학적 특성을 몬테카를로(Monte Carlo) 시뮬레이션 방법을 이용하여 고찰하였다. 섬광체는 NaI(TI) 결정체로 크기가 60 mm $\times$ 60 mm $\times$ 6 mm이며, 발생한 섬광이 광전자증배관의 광음극에 도달할 때까지를 시뮬레이션 하였다. 섬광체 표면 처리를(5가지 : Ground,. Polished, Metal-0.95RC(반사계수), Polished-0.98RC, Painted-0.98RC) 변화시켜 민감도를 계산하였다. 섬광체와 광전자증배관 (photomultiplier tube, PMT)의 접합면에 사용되는 물질의 굴절률 변화에 따른 민감도와 NaI(TI) 섬광체 창으로 많이 사용되고 있는 유리의 두께 변화에 따른 민감도를 조사하였다. 또한 섬광 결정체의 내인성 위치 분해능(intrinsic position resolution)을 섬광이 발생된 후 섬광 퍼짐(light spread) 정도를 시뮬레이션 함으로써 고찰하였다. 감마선의 입사면을 각각 Ground, Polished, Metal-0.95RC, Polished-0.98RC, Painted-0.98RC로 표면처리 하였을 때 민감도가 70.9%, 73.9%, 78.6%, 80.1%, 85.2%로 나타나 Painted-0.98RC일 때 민감도가 가장 우수하였다. 섬광체와 광전자증배관의 접합에 사용된 물질의 굴절률이 증가할수록 민감도가 증가하였으며 매질이 두꺼울수록 민감도는 저하되었다. 디자인된 검출기의 내인성 위치분해능은 수직, 수평 방향으로 약 1.2 mm로 예측되었다. 이 연구에서는 감마카메라의 성능에 직접적인 영향을 미치는 NaI (Tl)-PMT의 민감도와 위치 분해능을 몬테카를로 시뮬레이션을 통해 예견할 수 있었으며, 본 연구결과 NaI(TI)-PMT를 이용한 검출기가 고민감도ㆍ고해상력이 요구되는 유방암 진단용 소형 감마카메라 제작에 적합하다고 판단된다. We studied optical behavior of scintillation light generated in NaI(TI) crystal using Monte Carlo simulation method. The simulation was performed for the model of NaI(TI) scintillator (size: 60 mm ${\times}$ 60 mm ${\times}$ 6 mm) using an optical tracking code. The sensitivity as a function of surface treatment (Ground, Polished, Metal-0.95RC, Polished-0.98RC, Painted- 0.98RC) of the incident surface of the scintillator was compared. The effects of NaI(TI) scintillator thickness and the refractive index of light guide optically coupling between the NaI(TI) scintillator and photomultiplier tube (PMT) were simulated. We also evaluated intrinsic position resolution of the system by calculating the spread of scintillation light generated. The sensitivities of the system having the surface treatment of Ground, Polished, Metal-0.95RC, Polished-0.98RC and Painted-0.98RC were 70.9%, 73.9%, 78.6%, 80.1% and 85.2%, respectively, and the surface treatment of Painted-0.98RC allowed the highest sensitivity. As increasing the thickness of scintillation crystal and light guide, the sensitivity of the system was decreased. As the refractive index of light guide increases, the sensitivity was increased. The intrinsic position resolution of the system was estimated to be 1.2 mm in horizontal and vertical directions. In this study, the performance of NaI(TI)-PMT detector system was evaluated using Monte Carlo simulation. Based on the results, we concluded that the NaI(TI)-PMT detector array is a favorable configuration for small gamma camera imaging breast tumor using Tc-99m labeled radiopharmaceuticals.

반복된 유리체강내 베바시주맙 주입술 후 재발한 당뇨황반부종에 후테논낭하 트리암시놀론 주입술

김종호,박동호,신재필,김시열,Jong Ho Kim,Dong Ho Park,Jae Pil Shin,Si Yeol Kim 대한안과학회 2011 대한안과학회지 Vol.52 No.9

Purpose: To evaluate posterior sub-Tenon injection of triamcinolone acetonide (TA) for recurrent diabetic macular edema (DME) after repeated intravitreal bevacizumab (IVB). Methods: This is a retrospective interventional case series comprised of 35 eyes (32 patients) with recurrent clinically significant DME after two or more IVB treatments. All patients were administered TA 40 mg injections. Best-corrected visual acuity (BCVA), central macular thickness (CMT), and intraocular pressure (IOP) were reevaluated at months 1, 3, and 6. Results: The baseline OCT showed five eyes with diffuse retinal thickening, 18 eyes with cystoid macular edema, and 12 eyes with serous retinal detachment. Mean BCVA improved at months 1 and 3, and mean CMT decreased at months 1 and 3. However, mean BCVA and CMT at six months was not different from baseline. Each DME pattern showed significant improvements in BCVA and CMT at three months. Mean IOP at months 1, 3, and 6 did not show any significant difference from baseline. No eyes showed complications including cataract progression or endophthalmitis. Conclusions: Posterior sub-Tenon injection of TA should be considered for patients with recurrent DME after repeated IVB. The studied eyes showed improvements in BCVA and CMT during three months in all of the three patterns of DME. J Korean Ophthalmol Soc 2011;52(9):1063-1070

측두엽성간질의 간질병소 편측화에서 99mTc - HMPAO SPECT의 유용성 : 뇌파 , 자기 공명 영상 및 전산화 단층 영상과의 비교

김종호,김종순,김상은,최창운,이동수,정준기,이명철,고창순,이남수,명호진 ( Jong Ho Kim,Jong Soon Kim,Sang Eun Kim,Chang Woon Choi,Dong Soo Lee,June Key Chung,Myung Chul Lee,Chang Soon Koh,Nam Soo Lee,Ho Jin Myung,) 대한핵의학회 1991 핵의학 분자영상 Vol.25 No.1

TEC-less 비냉각 열영상 검출기용 소형카메라 모듈 개발

김종호,Kim, Jong-Ho 대한임베디드공학회 2017 대한임베디드공학회논문지 Vol.12 No.2

Thermal imaging is mainly used in military equipment required for night observation. In particular, technologies of uncooled thermal imaging detectors are being developed as applied to low-cost night observation system. Many system integrators require different specifications of the uncooled thermal imaging camera but their development time is short. In this approach, EOSYSTEM has developed a small size, TEC-less uncooled thermal imaging camera module with $32{\times}32mm$ size and low power consumption. Both domestic detector and import detector are applied to the EOSYSTEM's thermal imaging camera module. The camera module contains efficient infrared image processing algorithms including : Temperature compensation non-uniformity correction, Bad/Dead pixel replacement, Column noise removal, Contrast/Edge enhancement algorithms providing stable and low residual non-uniformity infrared image.