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전혈 , 림프구와 백혈구 연층 각각의 방사성 동위원소 체외 조사후 배양을 이용한 생물학적 선량측정
김종호,이동수,최창운,정준기,이명철,고창순,김종순,김희근,강덕원,송명재 ( Jong Ho Kim,Dong Soo Lee,Chang Woon Choi,June Key Chung,Myung Chul Lee,Chang Soon Koh,Chong Soon Kim,Hee Geun Kim,Duck Won Kang,Myung Jae Song ) 대한핵의학회 1995 핵의학 분자영상 Vol.29 No.1
The purpose of this study was to establish mononuclear cell cultures such as lymphocytes or buffy coat for the biological dosimetry of in vitro irradiation of the radionuclide Tc 99m in order to exclude the effect of residual doses seen in the cultures of whole blood. Biological dosimetry of Tc-99m on cultured mononuclear cells at doses ranging from 0.05 to 6.00 Gy, by scoring unstable chromosomal aberrations(Ydr) observed in cultured lymphocytes, were performed using peripheral venous blood of healthy normal person. The results showed that; (1) Jn vitro irradiation of radioisotope in separated lymphocyte or buffy coat showed trace amount af residual doses of isotope after washing. Residual doses of isotopes are increased in proportion tn exposed time and irradiated dose without difference between I-131 anct Tc 99m. (2) We obtained these linear-quadratic dose response equations in lymphocyte and buffy coat culture after in vitro irradiation of Tc-99m, respectively (Ydr = 0,001949 D+ 0,006279B+ 0.000185; Ydr= 0.002531 D 0.003274 D+0.003488). In aonclusion, the linear quadrstic dose response equation from in vitro irradiation of Tc 99m with lymphocyte and buffy coat culture was thought to be useful for assessing Tc 99m indueed biological effects. And mononuclear cell cultures seem to be the most appropriate experimental rnodel for the assessment of biological dosimetry of internal irradiation of radionuclides.
김종호 ( Chong Ho Kim ),장미경 ( Mi Kyoung Jang ),추상규 ( Sang Kyu Choo ) 대한임상검사과학회 2002 대한임상검사과학회지(KJCLS) Vol.34 No.2
In this present study, we carried out plasma protein electrophoresis by a method for cellulose acetate membrane electrophoresis and sodiumdodecylsulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). Plasmas were isolated from patients with acute myelogenous leukemia (AML) and patients with chronic myelogenous leukemia (CML). The composition and variation of the electrophoreticprotein fractions were compared with those of normal patients. CML and AML patients showed a low concentration of albumin, 2-globulin, l-globulin and 2-globulin. The concentrations of 2-globulin and -globulin were high in CML plasma but low in AML plasma. l-globulin in the plasma of CML and AML patients was lower than normal. The results of the SDS-PAGE showed that the plasma isolated from AML and CML patients has a typical protein band (MW. 35 KD) and a thicker protein band (MW. 30 KD) than the protein band of normal patient``s plasma. These results suggested that it is possible to use these results for diagnosis of myelogenous leukemia. In addition, the two bands on SDS-PAGE make it interesting to study the possibility of the mechanisms of myelogenous leukemia.
김종호 ( Chong Ho Kim ),권봉규 ( Bong Kyu Kwon ),박영순 ( Young Soon Park ) 대한임상검사과학회 1998 대한임상검사과학회지(KJCLS) Vol.30 No.3
분자량이 약 :38-40 Kd되는 당단백으로서 glycosylphosphatidylinositol (GPD 꼬리로 세포 형 질막에 결합하고 있는 막단백의 하나인 엽산 수용체의 기능과 발현을 인후암 세포(KB), 난소암 세포 <IGROVU, 태반암 세포 (JAR), 신장암 세포 (CAKI-1, 뇌암 세포 (GH3), 유방암 세포 (MCF-7), 그리고 폐암 세포 (NCI-H520) 등에서 연구하기 위하여 mRNA 분포, 각 암세포 에서 발현된 엽산 수용체의 양과 기능, 그리고 엽산 결합능을 분석하여 비교하였고 각 암 환자로부터 채취한 혈청내에 존재하는 엽산 수용체를 분석하여 비교하였다. 많은 양의 엽산 수용체를 발현하는 KB, IGROV1, JAR 세포들은 정확하게 Processing되고 GPI 고리로 세포벽에 결합되어 있음이 3H-ethanolamine 실험과 PIPLC (phosphainositol phoaphalipase C) 효소처리 실험에 의하여 확인되었다. 뿐만 아니라 이들 세포들은 mRNA 양, 엽산 수용체의 양 그리고 엽산결합능에서 큰차이가 없었다. 그러나 신장암 세포 (CAKI-l), 뇌암 세포 (GH3), 유방암 세포 (MCF-7),그리고 폐암 세포 (NCI-H5W)들에서는 확인할만한 양의 엽산 수용체의 발현이 되지 않하였다. 암환자들의 혈청내 존재하는 엽산 수용체의 양을 분석하여 난소암환자를 진단하는데 활용 가능성올 확인하기 위하여 구강암환자, 난소암환자, 태반암환자 신장암 환자, 뇌암환자, 유방암환자, 그리고 폐암환자들로부터 채취한 혈청내의 엽산 수용체 단백질을 [1짝]-MOv19 항체를 사용하여 분석하였다. 난소암환자 (61%), 태반암환자 (00%) 그리고 구강 암환자 (40%) 집단에서는 다른 실험군보다 높은 양성율을 나타내었으나 난소암환자를 진단하거나 관리하는데 활용되는am로서 충분히 감도가 높지 않음은 알았다.
PCR에 의한 DNA 증폭에 미치는 온도와 Cycle 수
김종호 ( Chong Ho Kim ),신상희 ( Sang Hee Shin ) 대한임상검사과학회 2004 대한임상검사과학회지(KJCLS) Vol.36 No.1
In order to study the effect of temperature of denaturation, annealing and extension and cycles on amplification of DNA by PCR method, We isolated the hepatitis B virus DNA from hepatitis B patient blood and compared the density of DNA amplified by Reference PCR Program (denaturation at 94℃ for 30 sec., annealing at 60℃ for 1 min., extension at 72℃ for 1 min., holding at 72℃ for 5min., 30 cycles) that is usually used in laboratory to the density of DNA amplified by PCR program changed only the denaturation temperature or annealing temperature or extension temperature. We amplified about 341bp of hepatitis B virus DNA by Reference PCR Program from hepatitis patient blood, but the DNAs denatured at 72℃ or 60℃ were not detectable on photoradiography film. The DNA amplified at 37℃ of annealing temperature was not detectable, but the DNA annealed at 72℃ was detectable the lower density of DNA than the DNA amplified by Reference PCR Program. Each DNA amplified by PCR program changed only the extension temperature to 37℃ or 60℃ was almost same density as DNA amplified by Reference PCR Program. We compared the density of hepatitis B virus DNA amplified by Reference PCR Program for 30 cycles, 20 cycles, 10 cycles, and 5 cycles. The DNA cycled for 20 cycles was not amplified well as cycled for 30 cycles, but the DNA was detectable on the photoradiography film. The DNAs amplified for 10 cycles or 5 cycles were not detectable on photoradiorgaphy film. The concentration of hepatitis B virus DNA amplified in Reference PCR condition for 30 cycles, 20 cycles, 10 cycles, and 5 cycles were 72㎍/㎖, 83×10-3㎍/㎖, 27×10-6㎍/㎖, and nondetectable, respectively.
김종호 ( Chong Ho Kim ),권봉규 ( Bong Kyu Kwon ),박영순 ( Young Soon Park ) 대한임상검사과학회 1999 대한임상검사과학회지(KJCLS) Vol.31 No.2
사람 Folate 수용체 (human folate receptor, hFR)는 세포의 형질막 단백질로서 glycosylphospha -tidylinositol (GPI) 꼬리에 의하여 막과 결합되어 있으며 난소암 marker 단백질로 알려져 있다. 우리는 백혈병 환자의 진단에 활용할 수 있는 가능성을 확인 하기 위하여 백혈병 환자의 전혈과 혈장에 존재하는 엽산을 정량하고 혈액 세포의 엽산 수용체 발현 정도를 분석 비교하였다. 급성골수성 백 혈병 (acute myelogenous leukemia, AML) , 만성골수성 백 혈병 (chronic myelogenousleukemia, CML), 급성림프구성백혈병 (acute lymphocytic leukemia, AML) 그리고 만성림프구성백혈병(chronic lymphocytic leukemia, CLL) 환자 혈액 내의 엽산 농도를 비교한 결과 AML의 혈장 농도(1.7ngjm1) 와 전혈 농도(115ngjml) 가 실험한 다른 백혈병 환자의 혈액 농도보다 가장 낮은 농도를 나타내었고, 정상인의 혈장농도(8.1 ngjml) 와 전혈농도(450.2ngjml)보다 매우 낮은 결과를 나타내었다.뿐만아니라 골수성 백혈병환자 혈액 내의 엽산 농도가 림프구성백혈병환자 혈액 내의 농도보다 낮은치를 나타내었다. 엽산 수용체의 발현정도를 확인하기 위하여 1251 -folic acid 결합 시힘을 실시한 결과 AML과 CML 환자의 혈 액 세포는 Nasophaηngea1 epidermoid carcinoma cell 1ine (뼈 cell) 의 발현 정도보다 낮은 발현 농도를 나타내었으나 ALL과 CLL 환자와 정상인의 혈액 세포에서는 정량할 수 있을 정도로 발현되지 않았다.
김종호 ( Chong Ho Kim ),박석태 ( Seok Tae Park ),박혜림 ( Hyea Rim Park ),박승택 ( Seung Taeck Park ) 대한임상검사과학회 2005 대한임상검사과학회지(KJCLS) Vol.37 No.2
We compared the serum protein electrophoresis patterns in sera of diabetes patients (n=166) which showed more than 150 mg/dL of glucose. The concentrations of total protein, albumin, α1-globulin, α2-globulin, β -globulin and γ-globulin in sera of patients were 6.3±1.2 g/dL, 40.3±6.4%, 4.9±2.3%, 13.5±3.8%, 15.5±3.9%, and 25.9±5.8%, respectively. The frequency of patients showed lower levels of total protein, albumin, α1- globulin were 48.5%, 96.4%, and 33.7%. The frequency of patients showed higher levels of α2-globulin, β- globulin, and γ-globulin were 56.6%, 47.0%, and 90.4%, respectively. This data suggests that the high concentration of glucose in blood may damage the kidney and liver. Also, the decrease of albumin and α 1-globulin and increase of α2-globulin, β-globulin and γ-globulin shows that the concentration of glucose in blood will have an effect on the variation of serum protein electrophoretic patterns.
김종호 ( Chong Ho Kim ),정미라 ( Mi Ra Jung ),박상호 ( Sang Ho Park ) 대한임상검사과학회 2006 대한임상검사과학회지(KJCLS) Vol.38 No.3
Alzheimer``s disease (AD) is characterised neuropathologically by the accumulation of neuritic plaques and neurofibrillary tangles as well as by cerebrovascular amyloid deposition and neuronal cell loss. Current advances have shown the apolipoproteinE-epsilon 4 (ApoE4) allele to be highly associated with late-onset familial and sporadic Alzheimer``s disease (AD) in Western populations. The association of ApoE allele frequencies and dementia remains unknown in populations from many countries. We recently initiated a project to examine ApoE frequencies in non-demented healthy Koreans. Genomic DNA in hair root from a thousand persons was collected and ApoE gene type was investigated with the methods of polymerase chain reaction (PCR) and restriction fragment length polymorphism. A group of a thousand non-demented Koreans over the age of 40 years were found to be positive in 15.7% of the cases for ApoE4. AD and ApoE4 were closely related. ApoE epsilon 4 was a dangerous factor of AD and ApoE 4 allele made a contribution to the heterogenicity of AD.