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김정희(Jeong-Hee Kim),김기옥(Ki-Ok Kim),우종규(Jong-Gyu Woo),김대일(Daeil Kim),김창길(Chang Kil Kim) 한국원예학회 2008 한국원예학회 학술발표요지 Vol.2008 No.12
사과 국내 육성 품종인 ‘감홍’ 품종의 형질 전환 체계를 확립하고 ‘감홍’의 칼슘 결핍에 의한 생리 장해인 고두병이 방제된 형질 전환체를 육성하기 위하여 CAX1 유전자 전환을 실시하였다. 접종에 사용되는 절편체는 증식 배양 중인 신초의 유엽을 사용하는 경우가 발근 배양중인 신초의 유엽을 사용하는 것보다 높은 재분화율을 보여 효과적이었다. 항생제 첨가 재분화 선발 배지에서 재분화된 신초들을 대상으로 PCR을 실시한 결과 20개체에서 유전자 도입을 확인할 수 있었고, 이들을 Southern blot 분석한 결과 6개체에서 안정적으로 유전자가 도입되었음을 확인할 수 있었다. 또한 유전자의 정량적 발현 분석을 위해 real-time PCR을 실시한 결과 대조구에 비해 형질 전환체 모두에서 CAX1 유전자의 상대적 발현량이 높게 나타났다. 형질 전환체의 칼슘 분석결과, 대조구와 큰 차이가 없었는데 기내 배양 잎의 분석 결과이기 때문에 기내 대량 증식 후 순화 과정을 거쳐 포장에서 생육시켜 과실이나 수체 등 다른 식물체 부위에서의 칼슘 함량을 측정해야 할 필요가 있다고 판단되었다. Agrobacterium-mediated transformation system of Korean apple cultivar ‘Gamhong’ was established. Korean superior cultivars such as ‘Hongro’ and ‘Gamhong’ have rapidly become popular and occupied about 20% of the total apple growing area since the late 1990s.‘Gamhong’is late maturing cultivar with high sugar and juice content. Since it is prone to calcium deficiency, however, bitter pit occurs during the maturing season. The CAX1 gene playing the role of H<SUP>+</SUP>/Ca<SUP>2+</SUP> antiporter, increases Ca levels in several crops. The CAX1 gene was introduced into‘Gamhong’ by Agrobacterium tumefaciens LBA4404 harboring pBI121 to obtain its transformants without bitter pit problem. Regenerated transgenic lines were confirmed by polymerase chain reaction (PCR) analysis and Southern blot analysis of genomic DNA for the existence of CAX1 gene. Southern blot analysis of‘Gamhong’transformants showed that six putative transgenic lines were integrated with CAX1 gene in genomic DNA. CAX1 comparative expression levels of the transformants were higher than those of non-transformants evaluated by comparative quantification analysis using a real-time PCR. These six lines are currently being multiplied in vitro. Developed shoots will be transplanted to the isolated greenhouse after acclimation, while the physiological expression of fruit will be investigated during the bearing season.
온도범위에 따른 비침습적 자기공명 온도측정방법의 최적화
김종민,조영승,김정희,이철현,오창현,Kim, Jong-Min,Kumar, Suchit,Jo, Young-Seung,Park, Joshua Haekyun,Kim, Jeong-Hee,Lee, Chulhyun,Oh, Chang-Hyun 대한의용생체공학회 2015 의공학회지 Vol.36 No.6
Noninvasive temperature monitoring is feasible with Magnetic Resonance Imaging (MRI) based on temperature sensitive MR parameters such as $T_1$ and $T_2$ relaxation times, Proton Resonance Frequency shift (PRFs), diffusion, exchange process, magnetization transfer contrast, chemical exchange saturation transfer, etc. While the temperature monitoring is very useful to guide the thermal treatment such as RF hyperthermia or thermal ablation, the optimization of the MR thermometry method is essential because the range of temperature measurement depends on the choice of the measurement methods. Useful temperature range depends on the purpose of treatment methods, for example, $42^{\circ}C$ to $45^{\circ}C$ for RF hyperthermia and over $50^{\circ}C$ for thermal ablation. In this paper, MR thermometry methods using $T_1$ and $T_2$ relaxation times and PRFs-based MR thermometry are tried on a 3.0 T MRI system and their results are reported and compared. In addition, the scanning protocol and temperature calculation algorithms from $T_1$ and $T_2$ relaxation times and PRFs are optimized for the different temperature ranges for the purpose of RF hyperthermia and/or thermal ablation.
장린(Rin Chang),장영운(Young Woon Chang),이정일(Jung Il Lee),김병호(Byoung Ho Kim),김효종(Hyo Jong Kim),김영관(Young Kwan Kim),김정희(Jeong Hee Kim),동석호(Suk Ho Dong) 대한소화기학회 1993 대한소화기학회지 Vol.25 No.1
Adenoid cystic carcinoma(ACC) is well known in the salivary gland, but ACC of the esophagus is a relatively rare lesion which characteristically exibit more aggressive clinical behavior and intensive widespread metastasis. Although many authors think that this tumor arises from the submucosal esophageal gland, its histogenesis is still in dispute. We have recently experienced a 66-year-old man who had ACC of the esophagus with chief complaint of hematemesis. The tumor was discovered incidentally together with gastric ulcer by esophagogastroscopy. The tumor exhibited a large lobulated fungating appearance, morphologically similar to ACC in the salivary glands and localized in th submucosal layer. The patient is still alive and well 12 months after surgery.