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김정연 ( Kim Jung Yean ),황보경 ( Hwang Bo Kyung ),홍종화 ( Hong Jong Hwa ),김태수 ( Kim Tae Soo ) 한국구조물진단유지관리공학회 2018 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.22 No.1
In this study, structural behaviors such as ultimate strength and fracture shape of duplex stainless steel (STS329FLD) fillet-welded connections according to welding direction were investigated through monotonic tensile test. Main variables are weld length and welding direction against the loading direction. Specimens of SDTFW series (transverse to the loading direction) and SDLFW series (longitudinal to the loading direction) failed by tensile fracture and shear fracture, respectively. Ultimate strengths got higher with the increase of weld length and SDTFW series showed higher ultimate strengths than SDLFW series.
문성환 ( Sung Hwan Moon ),김정연 ( Jung Yean Kim ),김계성 ( Kye Seong Kim ) 한국조직공학과 재생의학회 2004 조직공학과 재생의학 Vol.1 No.2
Embryonic stem cells (ESC) are thought to be a powerful tool for cell replacement in degenerated or damaged cells, including those in central nerve system because ESC are known to be pluripotent and capable of differentiating into various cell types found in adult body. In general, ESCs are differentiated into specific cell lineages through the formation of embryoid body (EB). We demonstrated the direct differentiation of mouse embryonic stem cells (mESC) into neuronal cells in culture from mESC aggregation, which were cultured in suspension for only 48 hrs. To process the differentiation of ES aggregates, they were attached onto poly-L-ornithin and fibronectin coated plates (stage I). Then media was changed to early-differentiated media supplement N2 in DMEM/F12 (stage II), during aggregates were expanded fully. After 7 days, we changed media for late differentiation media supplement N2 and B27 in DMEM/F12 for 7 days (stage III). After total 15 days, differentiated cells were observed by immunnocytochemistry with neuronal lineage markers such as neuronal nuclei (NeuN), microtubule-associated proteins-2 (MAP2), and tubulin 3. In addition, RT-PCR analysis showed the gene expression of nestin, OTX2, MAP2. Nestin and OTX2 gene were detected in the neuronal precursor cells and then decreased in differentiated neuronal cells. However, expression of MAP2 gene expression was detected in late differentiated (stage III) cells. Taken together, newly developed neuronal differentiation system without EB formation may be efficient method to drive rapid differentiation mESC into neuronal cells.