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Myoglobin에 의한 급성 신부전에서 Oxygen Free Radical의 역할
김주섭 ( Joo Seop Kim ),정대윤 ( Dae Yune Jung ),김장만 ( Jang Man Kim ),조홍래 ( Hong Rae Cho ),배수동 ( Soo Tong Pai ),강구 ( Gu Kang ),신형식 ( Hyung Sik Shin ) 대한외상학회 1991 大韓外傷學會誌 Vol.4 No.2
Highly reactive oxygen metabolites have been known to have a role in several models of acute renal failure. Especially hydroxyl radicals have been proved to be important mediators by some previous authors. This study was aimed to determine the role of superoxide radical as well as hydroxyl radical and to compare the protective effects of both scavengers in myoglobinuric renal failure. Acute renal failure was induced by intramuscular injection of hypertonic glycerol (6mg/kg, half-dose in each hindlimb muscles) in rats. Rats treated with glycerol and hydroxyl radical scavenger, dimethylsulfoxide (DMSO), showed lower serum blood urea nitrogen (BUN) and creatinine level than rats with glycerol alone. And also significant protection was noticed histologically. Superoxide radical scavenger, superoxide dismutase (SOD), had a similar protective effects. In contrast, allopurinol, an inhibitor of xanthine oxidase, was not protective at all. The present study may suggest that both superoxide and hydoxyl radical playa role of tissue injury in myoglobin-induced renal failure. Beside xanthine oxidase, other souces of oxygen free radical may exist.
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소화관 기계적 폐쇄에 따른 Interstitial Cells of Cajal (ICC) 및 위장관운동의 변화
전제열(Jae-Yeoul Jun),정춘해(Choon-Hae Chung),유호진(Ho-Jin You),김경희(Kyung-Hee Kim),김장만(Jang-Man Kim),김기훈(Kee-Hune Kim),박도영(Do-Young Park),장인엽(In-Youb Chang) 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.5
Interstitial cells of Cajal (ICC)는 내장 민무늬근세포와 소화관 신경종말들과 밀접한 해부학적 연관성을 가지고 있으며, ICC가 소화관 연동운동에 있어서 소화관 연동운동의 향도잡이 역할을 할 뿐만 아니라, 소화관신경으로부터 민무늬근세포로 전해지는 신경전달을 매개, 조절한다고 알려져 있다. 최근 여러 소화관운동장애 질환에서 ICC의 감소 및 소실이 병인과 연관성이 있다는 주장들이 제기되는 실정이다. 이에 따라 저자들은 수술적으로 생쥐 작은창자를 부분폐쇄를 시킨 다음, 작은창자 근육층의 비대증을 유도한 후 소화관 운동의 변화 및 ICC의 형태학적 변화를 밝혀내고자 ICC에 대한 면역형광염색과 전기생리학적 실험을 병행하여 실시하였다. 작은창자를 부분폐쇄시키고 2주 후, 폐쇄부위보다 입쪽의 작은창자부위에서 육안적으로 창자의 직경이 증가하고, 광학현미경적으로 근육층의 비대가 나타나는 것을 관찰할 수 있었다. ICC에 대한 면역형광염색(c-kit staining) 결과, ICC 그물망이 폐쇄부위보다 입쪽으로 25 mm 정도까지 소실 또는 뚜렷하게 감소되는 것을 관찰할 수 있었으며, 이러한 형태학적 결과와 병행하여 전기생리학적으로도 서파의 소실 또는 감소가 나타남을 관찰할 수 있었다. 그러나 폐쇄부위보다 항문쪽에서는 ICC와 서파의 이상소견은 관찰되지 않았다. 본 실험 결과 소화관의 폐쇄에 따라 입쪽부위 ICC의 소실과 서파의 소실을 관찰할 수 있었다. 따라서 앞으로 ICC 구조를 변화하게 하는 분자생물학적 그리고 유전학적 신호체계에 대해 면밀한 분석을 할 경우 소화관운동장애의 발생원인 및 치료에 계기를 마련할 수 있을 것으로 사료된다. Interstitial cells of Cajal (ICC) are the pacemakers in gastrointestinal slow wave, and also transduce signal inputs from the enteric nervous system to smooth muscle. The abnormal motility corresponded to a lack or decreasing of ICC and a disruption of electrical slow waves. So we developed partial obstruction model in murine small intestine and investigated changes in the ICC networks and electrical activity in the obstructed bowel using c-kit immunohistochemistry and intracelluar electrophysiological techniques. Two weeks following the onset of a partial obstruction, the small intestine increased in diameter and muscular hypertrophy was developed oral to the obstruction site. ICC were absent or only weak at 1~25 mm oral to the occlusion site, and this disruption was accompanied by the loss of electrical slow wave. ICC networks and slow waves were normal appearance aboral to the clip. In conclusion, The present results showed that partial intestinal obstruction induced the loss of ICC networks and slow waves. These result will provide a valuable aid for understanding pathogenesis of intestinal motility disorder, and this model may be an important tool for evaluating genetic or molecular factor for the therapeutic opportunities of motility disorder in human.
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생쥐 위장관의 Interstitial cells of Cajal(ICC)에 대한 면역조직화학적 연구
김영철,차경훈,신무경,임건한,김주영,안병수,김장만,양경철,박도영,오재욱,장인엽 조선대학교 2003 The Medical Journal of Chosun University Vol.28 No.1
Background and Objectives : Interstitial cells of Cajal(ICC) are the pacemakers in gastrointestinal tract that modulates gastrointestinal motiliey and these cells also transmit neural input from enteric nerves to smooth muscles. Recent work on tissues from patients with motility disorders that suggest that loss or defect in ICC could be related to pathophysiology in human and animal models. Immunolabelling of ICC in intestinal wall is recently developed by using specific marker, anti-c-kit antibody. Immunohistochemistry was done for ICC network in attempt to provide a morphological basis for the mechanism regulating gastrointestinal motility Methods : Cryosection was done, and whole-mount preparations of mouse stomach, gastrointestinal tract were immunolabelled using the anti-c-Kit. Immunolabelled ICC networks were observed under a confocal laser scanning microscopy. Results : According to three dimensional reconstruction study, we found that the c-Kit-positive celluar networks were widely distributed in the gastrointestinal muscle (1) circular muscle layer(IC-IM), (2) myenteric plexus(IC-MY), (3) deep muscular plexus(IC-DMP) in ileum, (4) submucosal plexus(IC-SMP) and longitudinal muscle layer(IC-LM) in colon. Conclusion : The characteristic profiles of ICC celluar networks provide a morphological basis upon the mechanism regulating gastrointestinal motility. Additional studies for the enteric nerves-ICC interaction are need to evaluate the detailed roles of Icc in gastrointestinal tract.
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개 대뇌겉질에서 Platelet-Derived Growth Factor α-Receptor의 출생 후 발달에 관한 면역조직화학적 연구
윤영,안병수,김인정,양경철,박선홍,김기훈,박도영,김장만,문정석,장인엽,조하영 조선대학교 부설 의학연구소 2002 The Medical Journal of Chosun University Vol.27 No.1
Background and Objectives : The localization of platelet-derived growth factor-α receptor (PDGF-α R) was commonly restricted to oligodendrocyte progenitors during late embryonic and postnatal development. However, several studies recently demonstrated that mature neurons could also synthesize PDGF-α, Materials and Methods : In the present study, to analyze the distributional pattern of PDGF-αR during postnatal development of the canine cerebral cortex, we used immunohistochemistry on sections of canine brain tissue. Results : We found that neurons of various regions of cerebral cortex exhibited the immunoreactivity to PDGF-αR as early as postnatal day 0, and slightly decreased after postnatal day 14. Whereas neuronal PDGF-αR were maintained at all ages, the oligodendroglia-like expression of PDGF-αR could not be confirmed. Conclusion : The localization of PDGF-αR in immature and mature neurons supports the several roles of PDGF during development, protection and survival of neurons.
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