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      • KCI등재

        김규동의 시세계 연구

        김은영(Kim Eun-young) 국어국문학회 2010 국어국문학 Vol.- No.156

        Kim, Gyu-Dong is a very unusual poet who has transformed into a dynamic process about his poetry. He was a very well known modernist when he took an active part of the 1950's modernism coterie 'Hubangee . However, in the 1970's, he changed himself as a socialized poet. I took care that realistic attitude he had shown in the 1950's and studied about it. I found the special point where the modernist Kim, Gyu-Dong had met the realist himself is shown in the connection between the poetry and the film. As everyone knows, Kim, Gyu-Dong prefer the automatism of surrealism as a method writing poem. And Kim, Gyu-Dong also had an inclination for the film Avant-Garde. Because the automatism of surrealism and the film Avant-Garde have the common theoretical base with the Freud's psychoanalysis. A lot of poems written by Kim. Gyu-Dong in the Korean war period show us special feature taking surrealism technique and the sense of the film Avant-Garde. He tried out express about the desperation and pain of the Korean people lived in that same period with his poems. But unfortunately, some several poems lost the critical mind and fell to nihilism. Kim, Gyu-Dong wanted to resist to the Korean social contradictions and conflicts continuedly. So he of course chose the more realistic writing method and concerned with the film Neo-Realism. He described some kind of the Nee-Realism character and circumstance in his poems for example 〈acrobat〉, 〈small hand〉. He described them as like using camera of the Neo-Realism film for presentation the problem of Korean 1950's society and community. And That's why he was very interested on the film Neo-Realism. So we can realize the true that his 1950's poetry had obvious inclination of realism already. It explains us about the connection between Kim, Gyu-Dong s 1950's poetry and 1970's poetry.

      • 소아의 소장 중첩증의 치료 : 자연 정복 vs 수술적 치료

        김은영,정규환,박태진,박귀원,정성은,김현영,Kim, Eun-Young,Jung, Kyu-Whan,Park, Tae-Jin,Park, Kwi-Won,Jung, Sung-Eun,Kim, Hyun-Young 대한소아외과학회 2010 소아외과 Vol.16 No.2

        Unreduced small bowel intussusception requires operative treatment although the rate of spontaneous reduction is 60 to 70 %. The aim of this study is to compare clinical characteristics and outcome between spontaneous reduction and operation group and to analyze factors related to decisions to treat small bowel intussusceptions. The records of 25 patients with small bowel intussusceptions treated in Seoul National University Children's Hospital from January 1999 to August 2009 were reviewed respectively. Spontaneous reduction group (n=12, 48 %) had signs and symptoms of vomiting, abdominal pain, currant jelly stool, abdominal distension, fever, increased CRP but no rebound tenderness. One of them had been diagnosed with Henoch-Schonlein purpura and no one displayed pathologic leading point by image study. Operation group (n=13, 52 %) consisted of patients who had primary surgery. Their signs and symptoms were similar to spontaneous reduction group. Seven of them had underlying diseases such as Crohn' disease, ALL, Lymphoma, Peutz-Jeghers syndrome (n=3), post-transplanted state of liver and 2 of them displayed Peutz-Jeghers polyp and Meckel's diverticulum as pathologic leading point by preoperative ultrasonography. Mean relieve interval (interval between onset of symptoms and reduction/operation) was 1.78 days in spontaneous reduction group and 2.25 days in operation group (p=0.341). Seven of operation group had manual reduction and 6 out of 7 received segmental resection of the small bowel. No one of them underwent manual reduction and all of them underwent segmental resection were found to have pathologic leading points [Peutz-Jeghers polyp (n=3), Meckel's diverticulum (n=2), lymphoma (n=1)] during operation. In conclusion, 48% of small bowel intussusceptions resolved spontaneously. Patients' symptoms and relieve intervals were not related to the operative decisions. We therefore recommend significant factors for determining treatment plan such as change of clinical symptoms, underlying disease or pathologic leading point by imaging.

      • SCOPUSKCI등재

        동결-융해된 인간 배반포기 배 유래의 배아 간(幹) 세포 배양

        김은영,남화경,이금실,박세영,박은미,윤지연,허영태,조현정,박세필,정길생,임진호,Kim, Eun-Young,Nam, Hwa-Kyung,Lee, Keum-Sil,Park, Sae-Young,Park, Eun-Mi,Yoon, Ji-Yeon,Heo, Young-Tae,Cho, Hyun-Jung,Park, Se-Pill,Chung, Kil-Saeng,Lim, Jin 대한생식의학회 2001 Clinical and Experimental Reproductive Medicine Vol.28 No.1

        Objective: This study was to establish the human embryonic stem (ES) cells derived from frozen-thawed blastocyst stage embryo that were destined to be discarded after five years in routine human IVF-ET program. Methods: Frozen-thawed and survived human blastocysts were treated by immunosurgery, and recovered ICM cells were cultured onto STO feeder cell layer and ICM colony was subcultured by mechanical dissociation into clumps. To identify ES cell, alkaline phosphatase staining and expression of Oct4 in replated ICM colonies were examined. Also, to examine the possibility of ES cell differentiation, retinoic acid (RA), basic fibroblast growth factor (b-FGF), nerve growth factor (NGF) were added in culture medium. In addition, to classify the specific cell type, differentiated cells were stained by indirect immunocytochemistry. Results: One ICM colony recovered from frozen-thawed six blastocysts was subcultured, continuously replated during 40 passage culture duration without differentiation. Subcultured colonies were strong positively stained by alkaline phophatase. When the expression of Oct4 in cultured ES colony was examined, Oct4b type is more clearly indicated than Oct4a one although there was not detected in embryoid body or differentiated cells. In differentiated cardiomyocytes from ES colony, cells were beaten regularly (60 times/min). In differentiated neural cells from ES colony, neurofilament (NF) 200 kDa protein, microtubule associated protein (MAP) 2 and ${\beta}$-tubulin of specific marker in neurons, glial fibrillary acidic protein (GFAP) of specific marker in astrocytes and galactocelebrocide (GalC) of specific marker in oligodendrocytes were confirmed by indirect immunocytochemistry. Also, muscle cells were detected by indirect immunocytochemistry. In addition, ES colonies can be successfully cryopreserved. Conclusion: This study suggested that establishment of human ES cells can be successfully derived from frozen-thawed blastocysts that were destined to be discarded, and obtained specific cell types (cardiomyocytes, neurons and muscle cells) through the in vitro differentiation procedures of ES cells.

      • KCI등재후보

        소아 비만에서 Leptin과 골대사의 연관성

        김은영,노영일,양은석,문경래,박상기,박영봉,이영화,Kim, Eun Young,Rho, Young il,Yang, Eun Seok,Moon, Kyung Rae,Park, Sang Kee,Park, Yeong Bong,Lee, Young Hwa 대한소아소화기영양학회 2006 Pediatric gastroenterology, hepatology & nutrition Vol.9 No.2

        목 적: 체중과 지방량이 골밀도와 밀접한 연관이 있는 것으로 알려져 왔다. 비만아에서는 지방 조직에서 합성 분비되는 leptin이 증가되는데, 증가된 leptin이 비만아의 골 대사에 미치는 영향에 대해 알아보고자 연구를 하였다. 방 법: 2005년 11월부터 2006년 5월까지 조선대학교병원 외래를 방문한 50명의 남자(비만아 25명, 정상 체중의 대조군 25명)를 대상으로 하였다. 체질량 지수, 체지방률, 삼두박근 피부주름 두께와 견갑골하 피부주름 두께를 측정하고 혈청 leptin과 골대사 지표인 B-ALP, CICP 및 total DPD 농도를 검사하였다. 비만군과 대조군의 신체 계측, leptin 및 골대사 지표 농도를 비교하고, leptin과 골대사 지표와의 상관성을 알아보았다. 결 과: 비만군에서 체질량 지수(r=0.551, p<0.05), 체지방률(r=0.584, p<0.05)과 유의한 양의 상관관계가 있었다. Leptin은 골형성 지표인 B-ALP (r=-0.613, p=0.026) 및 CICP (r=-0.583, p=0.037)와 유의한 음의 상관성을 보였다. 그러나 골흡수 지표인 total DPD (r=0.103)와는 유의한 상관성이 없었다. 대조군에서 leptin과 유의한 상관성이 있는 인자는 없었다. 골대사에 영향을 주는 체중을 통제하고 상관성을 알아본 결과에서도 비만군에서 leptin은 B-ALP (r=-0.728, p=0.007) 및 CICP (r=-0.684, p=0.014)와 유의한 음의 상관성을 보였으나 total DPD와는 연관성이 없었다. 대조군에서는 leptin과 연관성 있는 골대사 지표는 없었다. 비만군에서 골대사 지표에 영향을 미치는 인자에 대해 다중 단계별 회귀 분석을 시행한 결과 종속변수인 B-ALP는 leptin과 유의한 음의 상관성을 보였으며(Y=-39.653X+356.341, p=0.026), CICP도 leptin과 유의한 음의 상관성을 보였다(Y=-13.437X+116.013, p=0.037). 결 론: 비만아에서 leptin은 골형성 지표인 B-ALP 및 CICP와 음의 상관성이 있었으며, 골 흡수가 아닌 골 형성을 저하시키는 것을 알 수 있었다. Purpose: The aim of this study was to evaluate the influence of leptin on biochemical markers of bone metabolism in childhood obesity. Methods: A total of 50 male children (25 obese and 25 controls) were recruited from the pediatric outpatient clinic at the Chosun University Hospital from November 1st 2005 to May 30th 2006. BMI, body fat percentage, serum leptin, bone-specific alkaline phosphatase (B-ALP), C-terminal propeptide of type 1 collagen (CICP), total deoxypyridinoline crosslinks (total DPD) were measured. The correlations of leptin with BMI, body fat percentage, B-ALP, CICP, total DPD were analyzed by Pearson's correlation. In a multiple stepwise regression analysis, leptin after correction for body weight was evaluated if there was a correlation with biochemical markers of bone formation and resorption respectively. Results: The leptin levels of the obese group were significantly higher than those of the control group (p=0.012). In the obese group, the leptin level was significantly positively correlated with the BMI (r=0.551, p=0.01) and the percentage of body fat (r=0.584, p=0.018). In the obese group, of bone markers, B-ALP (r=-0.613, p=0.026) and CICP (r=-0.583, p=0.037) were negatively correlated with leptin. B-ALP (r=-0.728, p=0.007) and CICP (r=-0.684, p=0.014) were negatively correlated with leptin when corrected for body weight. In the control group, bone markers were not correlated with leptin. In the multiple stepwise regression analyses, there was a negative correlation between the leptin and B-ALP (Y=-39.653X+356.341, p=0.026), CICP (Y=-13.437X+ 116.013, p=0.037) respectively in the obese group. Conclusion: Leptin was a significant factor in the bone formation but not in bone resorption in childhood obesity.

      • A New Efficient Cryopreservation of Human Embryonic Stem Cells by a Minimum Volume Cooling Method

        김은영,박세영,윤지연,길광수,이창현,이건섭,태진철,김남형,이원돈,정길생,박세필,임진호,Kim, Eun-Young,Park, Sae-Young,Yoon, Ji-Yeon,Ghil, Gwang-Su,Lee, Chang-Hyun,Lee, Gun-Soup,Tae, Jin-Cheol,Kim, Nam-Hyung,Lee, Won-Don,Chung, Kil-Saeng,Pa The Korean Society for Reproductive Medicine 2004 Clinical and Experimental Reproductive Medicine Vol.31 No.1

        연구 목적: 본 연구는 인간배아줄기세포 동결에 minimum volume cooling (MVC) 초자화 동결방법이 유용하게 이용될 수 있는지의 여부를 조사하고자 실시하였다. 연구재료 및 방법: 인간배아줄기세포 콜로니는 0.05% collagenase 처리와 기계적 처리에 의해 작은 clumps로 자른 다음 동결 방법에 따른 효율을 비교 검토하고자, i) 대략 40-50개의 clumps를 10% DMSO가 들어있는 동결액에 $5{\sim}10$분간 처리하여 1ml cryo-vial에 넣고 slow-cooling용 cryo-module에 장착하고 -80C에서 overnight한 후 $LN_2$에 침지하여 완만동결을 실시하거나 ii) 10% ethylene glycol (EG)이 들어 있는 동결액에서 5$\sim$10분 처리하고 30% EG과 0.5 mol sucrose가 들어 있는 동결액에서 30초간 처리하여 본 연구를 위해 개발된 MVC straw에 10 clumps씩 적재한 다음 $4{\sim}5$ MVC straw를 $LN_2$가 들어있는 cryo-vial에 넣어 MVC 초자화동결을 실시하였다. 융해 후 생존율을 조사하였고 배아줄기세포의 특성을 유지하고 있는지의 여부를 조사하였다. 결 과: 융해 후, 인간배아줄기세포의 생존율은 완만동결을 실시했던 군 (20.0%) 보다 MVC 초자화동결을 실시했던 군에서 (76.0%) 유의하게 높게 나타났다. 또한 회복과정에서 완만동결을 실시했던 군에서는 세포성장이 매우 더디고 안 좋은 반면, MVC 초자화동결을 실시했던 군은 배아줄기세포의 증식이 동결을 실시하지 않은 세포와 같은 상태로 2주 이후부터 빠르게 전환되고 회복되는 것을 확인할 수 있었다. 이와 더불어 MVC 초자화동결-융해 후 회복된 배아줄기세포에서 정상 핵형, alkaline phosphatase acitivity, SSEA-4와 TRA-1-60 염색 및 Oct-4 발현을 확인하였으며 체외분화의 특성도 확인하였다. 결 론: 새로이 개발된 MVC 초자화동결을 이용하면 인간배아줄기세포는 고유의 특성을 잃지 않고 성공적으로 동결될 수 있다.

      • KCI등재

        수원 지방에서 조명나방 휴면과 발생 세대수 및 휴면유도에 대한 유충의 민감성

        김은영,김이현,서보윤,김용균,박창규,정진교,Kim, Eun Young,Kim, I Hyeon,Seo, Bo Yoon,Kim, Yonggyun,Park, Chang-Gyu,Jung, Jin Kyo 한국응용곤충학회 2020 한국응용곤충학회지 Vol.59 No.3

        수원 지방에서 조명나방(Ostrinia furnacalis)(나비목: 포충나방과) 성충 발생횟수와 유충 휴면이 유도되는 시기를 분석하였다. 성충은 일년에 3회 발생 하였는데, 1화기는 5월 초부터 7월 초까지, 2화기는 7월 초중순부터 8월 초중순까지, 3화기는 8월 중순부터 10월까지이었다. 7월말부터 8월 중순 사이에 갓부화 유충을 야외에서 사육하기 시작하였을 때, 그 해와 이듬해 용화한 개체들이 같이 발생하였다. 이후 사육된 집단에서는 모두 월동 유충들만 출현하였다. 야외에서 7월과 8월 중 채집된 유충들에서는 월동하거나 월동하지 않는 개체들이 같이 발생하였고, 이후 채집된 개체들에서는 모두 월동하는 개체들만 나타났다. 이 결과들은 수원에서 조명나방은 연중 2세대와 3세대를 경과하는 개체들이 같이 발생하는 복합 생활사를 갖는 것을 보였다. 실험실에서, 조명나방 유충을 영기별로 탈피 직후(9시간 이내)에 휴면유도 조건(11:13 시간 = 명:암 광주기, 20℃)에 처음 처리하였을 때, 거의 모든 유충들에서 휴면이 유도되었다. 유사한 처리를 5령으로 탈피한 유충 나이별로 적용하였을 때, 5령 3일과 4일 나이의 유충에 대한 처리에서는 휴면유도율이 감소하였다. 한편 알에서 갓 부화했을 때부터 휴면유도 조건에서 사육하던 유충들을 3령, 4령, 5령 탈피 직후에 비휴면 조건으로 옮겼을 때, 거의 대부분의 유충들은 휴면에 유도되지 않았다. 그 결과로부터 5령 초기 나이의 유충들이 휴면유도 자극에 반응하는 최종 단계라고 추정되었다. 비휴면 유충들에 비해, 휴면이 유도된 유충들에서는 혈림프 내 트레할로스 함량이 증가하였고, 몸체 과냉각점이 낮아졌다. The diapause induction season in Ostrinia furnacalis (Lepidoptera: Crambidae) was estimated in Suwon. Three batches of adult generations were observed, the first one from early May to early July, the second from early or mid-July to early or mid-August, and the third from mid-August to October. In outdoor larval rearing, colony rearing occurring from mid-July to mid-August produced both non-overwintering and overwintering larvae, whereas late-reared colonies produced only overwintering larvae. Larvae collected during July and August in maize fields produced both non-overwintering and overwintering larvae, whereas late-collected larvae produced only overwintering larvae. The results indicated that O. furnacalis has a bi- or trivoltine complex life cycle in this area. In the laboratory, when larvae of all instars within 9 h after molting were first treated to a diapause induction condition (11:13 h = light:dark photoperiod and 20℃), almost all larvae were induced to diapause. However, when similar treatments were conducted age-specifically for the 5th instar larvae, diapause induction rates in 3- and 4-day-old larvae of the 5th instar decreased. In contrast, when larvae were subjected to the diapause induction treatment only during the periods from the hatching stage to the 2nd, 3rd, and 4th instar, almost all larvae were not induced to diapause. The results suggest that the early age of the 5th larval instar is the last stage for sensitivity to diapause induction stimuli. In the diapause-induced larvae, hemolymph trehalose content increased and body supercooling points dropped, compared with those in non-diapause larvae.

      • KCI등재

        동화(同化)와 이화(異化)의 문화정치, 1925년의 음악계

        김은영 ( Kim Eun Young ),신혜승 ( Shin Hye Seung ) 이화여자대학교 음악연구소 2021 이화음악논집 Vol.25 No.4

        본고는 1920년대 문화통치기의 음악사회를 고찰하였다. 3.1운동 이후 일제는 식민통치의 방식을 무단통치에서 문화정치로 바꾸었고 조선어 신문의 발행을 허용했다. 민족주의자들은 신문 및 잡지를 정치적 담론의 공간으로 활용하는 한편, 일제에 의해 허용된 범위 내에서 문화운동을 전개하였다. 1920년대의 문화운동은 특히 일본 유학생 출신의 지식인들이 주도하였고 서구 근대예술을 모델로 하는 신문화운동의 전개와 사회주의의 영향을 받은 예술 활동을 특징으로 하고 있다. 식민지 지식인들의 문화운동에 김영환, 홍난파, 윤심덕, 한기주 등 동경 유학파 출신 음악가들의 참여도 있었다. 본고에서는 1925년의 음악회를 중심으로 1920년대의 음악사회를 고찰하였다. 1925년은 최초의 음악전문잡지 『음악계』가 출판된 해로, 홍난파는 전문음악회를 표방하였다. 그가 생각하는 “음악회다운 음악회”는 동정음악회나 자선음악회와 같은 사회구제 성격의 음악회가 아닌 ‘음악을 위한 음악회’, 다시 말해 서구음악을 주요 레퍼토리로 하는 음악회였다. 그러나 1925년의 대홍수로 한반도는 엄청난 피해를 입었고 이 사건으로 일제의 동화정책의 허구와 민족차별의 실체가 드러나게 된다. 수해구제를 위한 음악회는 경성을 넘어 전국에 걸쳐 개최되었으며 당국은 수해구제를 명시하는 음악회를 금지함으로써 음악회는 민족 공동체의 일체감을 형성하는 중요한 매개체가 된다. 1920년대 음악회의 또 다른 양상으로 음악무도의 유행이 있었다. 천도교 소년회에서 시작된 어린이/소년운동은 사회주의의 영향으로 건강한 신체의 단련을 표방했고 음악무도는 이 시대를 특징짓는 하나의 음악현상이 되었다. 음악무도의 유행은 1925년의 음악회에도 반영되어 당대의 대표적 음악가들이 참여한 가운데 어린이 및 소년회, 각 교회의 주일학교, 유치원 등에서 주최하는 경우가 많았다. 1925년의 음악회를 통해 문화통치기 일제의 동화주의 정책의 이면에는 배제와 차별의 이화(異化)가 존재했음을 확인할 수 있다. 일제가 문화통치를 위해 노린 것은 민족주의 내부의 분열과 이를 통한 친일파의 양성에 있었고 음악계도 문화통치의 영향에서 벗어날 수 없었다. This paper examines the music society under the cultural rule during 1920s. After the March 1st Movement, the Japanese changed the way of colonial rule to cultural politics and allowed the publication of Korean newspapers. Nationalists used newspapers and magazines as spaces for political discourse, while carrying out cultural movements within the limits allowed by the Japanese. The cultural movement of the 1920s was especially led by intellectuals who studied in Japan, and was characterized by the development of a new cultural movement modeled on Western modern art and artistic activities influenced by socialism. Musicians from Tokyo, such as Kim Young-hwan, Hong Nan-pa, Yoon Shim-deok, and Han Ki-joo, also participated in the cultural movement led by colonial intellectuals. This paper examines the music society during 1920s, mainly focusing on music concerts in 1925. 1925 was the year when the first music magazine ‘The Musical World (Um-Ak-Gye)’ was published, and Hong Nan-pa advocated a professional music concert. He thought of “concert-like concerts” as “concerts for music,” in other words, a concert with Western music as its main repertoire, not a concert of social relief such as sympathetic concerts or charity concerts. However, it was also the year when the great flood in 1925 that caused tremendous damage to the Korean Peninsula, revealing unsubstantiality of imperialism’s assimilation policy and national discrimination of Japan. Concerts about flood relief took place across the country beyond Gyeongseong, and by banning concerts specifying flood relief, concerts become an important medium for forming a sense of unity in the national community. There was a trend of music and dance, as another aspect of music concerts during 1920s. The children’s/boys movement, which began at the Cheondogyo Juvenile Association, advocated the training of a healthy body, and music and dance became a musical phenomenon characterizing this era. Music concerts in 1925 were hosted by children’s and juvenile associations, Sunday schools of each church, and kindergartens. The participation of all musicians active at the time stands out. Through the concert in 1925, it was confirmed that there was a politics of exclusion and discrimination behind the assimilationist policy of the Japanese colonial rule. What the Japanese imperialism aimed at for cultural rule was the division within nationalism and was to make pro- Japanese group through it, and the music community could not escape from the influence of cultural rule. History proves this.

      • KCI등재

        세포외 Protease를 생산하는 내염성 Bacillus sp. SJ-10 균주의 분리 동정 및 효소 특성

        김은영,김동균,김유리,최선영,공인수,Kim, Eun-Young,Kim, Dong-Gyun,Kim, Yu-Ri,Choi, Sun-Young,Kong, In-Soo 한국미생물학회 2009 미생물학회지 Vol.45 No.2

        오징어 젓갈로부터 호염성 균주를 분리하여 형태적, 생리 생화학적 특성 및 분자계통학적 분석을 통하여 동정하였고, 이 균주가 세포외로 생산하는 호염성 protease를 정제하여 그 특성을 분석하였다. 본 균주는 NaCl 0~14%, $20\sim55^{\circ}C$ 및 pH 5~8에서 성장하였으며, $35{\pm}5^{\circ}C$, pH 7 및 5% NaCl에서 최적으로 성장하였다. 주요 지방산 조성은 anteiso-$C_{15:0}$ (44.70%), anteiso-$C_{17:0}$ (15.76%) 및 $C_{16:0}$ (13.91%)이었고, menaquinone은 MK-7이었으며, DNA G+C 함량은 50.58 mol%였다. 16S rRNA 유전자 염기서열을 통한 계통분석 결과 Bacillus이었으며 Bacillus sp. SJ-10으로 명명하였다. SJ-10 균주 배양액으로부터 40% 황산암모늄 침전과 Mono Q 컬럼크로마토그래피 과정을 거쳐 collagen과 gelatin을 특이적으로 분해하는 약 40 kDa의 세포외 protease를 정제하였다. 이 효소는 $35{\pm}5^{\circ}C$, NaCl $5{\pm}1%$ 및 pH 8에서 최적 활성을 나타냈으며, pH 5~10 범위에서 안정하였다. A bacterium producing the halotolerant extracellular protease was isolated from squid jeotgal, and was identified as Bacillus sp. SJ-10 based on morphological, physiological and biochemical characteristics, as well as phylogenetic analysis using 16S rRNA gene sequence. The strain grew at $20^{\circ}C\sim55^{\circ}C$, pH 5~8, and 0%~14% NaCl and optimal growth conditions were $35{\pm}5^{\circ}C$, pH 7, and 5% NaCl. The major cellular fatty acids were anteiso-$C_{15:0}$, anteiso-$C_{17:0}$, and $C_{16:0}$ DNA G+C content was 50.58 mol% and menaquinone consisted of MK-7 Phylogenic analysis based on the 16S rRNA gene sequence indicated that SJ-10T belongs to the genus Bacillus. About 40 kDa of the salt-tolerant protease was purified by 40% ammonium sulfate saturation and Mono Q column chromatography. The optimal activity of the protease was pH 8 and stable at pH 5~10. The optimum temperature and NaCl concentration were $35{\pm}5^{\circ}C$ and $5{\pm}1%$, respectively.

      • KCI등재

        폐(廢) 적층형(積層形)세라믹콘덴서에 함유(含有)된 니켈의 침출거동(浸出擧動)

        김은영,김병수,김민석,정진기,이재천,Kim, Eun-Young,Kim, Byung-Su,Kim, Min-Seuk,Jeong, Jin-Ki,Lee, Jae-Chun 한국자원리싸이클링학회 2005 資源 리싸이클링 Vol.14 No.5

        폐 적층형세라믹콘덴서(Multi-Layer Ceramic Capacitor, MLCC)에 함유된 니켈의 침출거동에 대한 연구를 수행하였다. 침출제인산의 종류와 산농도, 침출온도, 입자크기, 침출시간이 니켈의 침출거동에 미치는 영향을 조사하였다. 실험결과, 광액농도 5g/L, 질산농도 1N 침출온도 $90^{\circ}C$, 시료입도 $-300/+180{\mu}m$, 침출시간 30분에서 니켈의 침출율은 97%이었다. 니켈의 침출반응속도는 cylinder 형상의 Jander 식에 잘 따르는 것으로 분석되었다. 니켈의 침출반응은 잔류 $BaTiO_3$ 공극 확산율속반응이며, 활성화 에너지는 37.6kJ/mol(9.0 kcal/mol)이였다. Leaching behavior of nickel contained in waste Multi-Layer Ceramic Capacitor (MLCC) was investigated using a batch reactor. The effects of acid type, acid concentration, leaching temperature, particle size, and reaction time on the extraction of nickel metal from waste MLCC were examined. As a result, 97% of nickel contained in waste MLCC was leached out in 30 min at the temperature of $90^{\circ}C$ under the condition of $HNO_3$ concentration 1N, solid/liquid ratio 5 g/L and particle size $-300/+180{\mu}m$. It was also found that a Jander equation was useful to fit well the leaching rate data. The rate of nickel leaching is controlled by pore diffusion in $BaTiO_3$ layer and has an activation energy of 37.6 kJ/mol (9.0 kcal/mol).

      • KCI등재후보

        태음인 청폐사간탕이 lipase 활성저해 및 db/db mouse의 체중감량, 혈장지질, UCP 1, 2 발현에 미치는 영향

        김은영,설유경,최재중,전수형,김훈,김종원,Kim, Eun-Young,Sul, Yu-Kyung,Choi, Jae-Jung,Jeon, Soo-Hyung,Kim, Hoon,Kim, Jong-Won 사상체질의학회 2007 사상체질의학회지 Vol.19 No.1

        1. Objectvies This experimental study was designed to investigate the effect of cheongpesagan-tang extract on the obstruction of the lipase activity and weight, plasma, UCP1, 2 mRNA in db/db mouse. Material and Methods: The body weight loss, food intake, feeding efficiency ratio, weight of the internal organs (liver, kidney, epididymal fat, brown adipose tissue), plasma glucose, triglyceride, total cholesterol, white adipose tissue, adipocyte size distribution, expression of UCP1, 2 mRNA were measured in db/db mouse administered Cheongpesagan-tang extract for 6 weeks. These were then compared with those of control groups administered the diet. 2. Results 1) Inhibitory effect against lipase activity was Kilgyung(81.7%), Nabokja (73.1%), Seungma(73.0%), Daewhang (68.4%), Kalgeun (55.3%), Kobon(34.5%), Hwanggeum(4.2%). 2) In the sample group, the body weight was significantly decrease than that of control group. 3) In the sample group, the weight of epididymal fat showed significantly decrease than that of control group. 4) In the sample group, triglyceride showed significantly decrease than that of control group. 5) In the sample group, distribution of adipose tissue showed significantly larger than that of control group. 6) In the sample group, UCP1, 2 mRNA in BAT showed significantly increase than that of control group. 3. Conclusions These results show that cheongpesagan-tang has an effect on the treatment of obesity.

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