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모발 개선을 위한 Transglutaminase의 적용
김윤석 ( Yun Seog Kim ),박수진 ( Su Jin Park ) 대한화장품학회 2013 대한화장품학회지 Vol.39 No.1
단백질을 연결시키는 효소인 transglutaminase는 모발에서 다양한 효과를 나타낼 수 있는 가능성이 있는데 이중에는 모발의 단단함을 증가시키는 작용도 포함된다. 여러 transglutaminase 효소 중 Streptomyces mobaraensis로부터 분리된 미생물 유래 효소를 손상된 모발에 사용한 후 인장강도를 평가한 결과 초기에 비해 15.64%까지 증가되는 것이 확인되었다. 이러한 효과는 transglutaminas가 샴푸를 사용하여 세정하는 과정에서의 모발 손상을 복구시킬 수 있다는 것을 의미한다. 또한 transglutaminase를 이용해 모발 표면의 특성을 개선 시킴으로써 모발의 윤기를 증가시키고 표면의 마찰력을 감소시키는데 유용하게 이용될 수 있었다. The use of protein-crosslinking enzyme, transglutaminase, as a biocatalyst in the processing of hair offers a variety of exciting and realistic possibilities which include improving the rigidity of hair fibers. Among the transglutaminases from many different living organisms, the microbial enzyme prepared from Streptomyces mobaraensis, significantly increased the tensile strength of hair by 15.64% compared to a control when it was applied to damaged hair. This indicates that transglutaminase can restore the negative effects of washing hair with shampoo. Also transglutaminase improved the characteristics of hair surface, which could be useful for increase of luster and reduction of friction force of hair surfaces.
Curcuma xanthorrhiza 추출물 및 함유 치약의 구취 억제 효과와 구강 유해균에 대한 선택적 항균 효과
김백일 ( Baek Il Kim ),김상년 ( Sang Nyun Kim ),장석윤 ( Sug Youn Chang ),문교태 ( Kyo Tae Moon ),김윤석 ( Yun Seog Kim ),황재관 ( Jae Kwan Hwang ),정승화 ( Seung Hwa Jeong ),김민영 ( Min Young Kim ),김해선 ( Hae Sun Kim ),권호근 대한예방치과·구강보건학회 2005 大韓口腔保健學會誌 Vol.29 No.2
Biosurfactant 를 생산하는 Acinetobacter calcoaceticus CL 의 분리 및 Biosurfacatant 의 생산과 응용
황경아,김윤석,안호정,최규석 ( Kyung A Hwang,Yun Seog Kim,Ho Jeong Ahn,Kyu Suk Choi ) 한국공업화학회 1995 공업화학 Vol.6 No.4
활성오니와 하천수로부터 분리한 탄화수소 자화성 미생물 중 배양액의 유화능과 표면장력 저하능이 우수한 균주 1종을 선별하여 자동동정기기를 통해 동정한 결과 Acinetobacter calcoaceticus였다. 본 균주는 수용성 탄소원 및 각종 탄화수소와 식물성 oil 등 다양한 탄소원을 이용할 수 있으며 이 중 ethanol 2%에서 biosurfactant의 생산성이 우수하게 나타났다. 질소원으로서는 urea를 효과적으로 이용하며 어느 정도 질소가 제한된 상태(C/N ratio=6.8)에서 biosurfactant의 생산성이 높게 나타났다. 현재 국내외에서 시판중인 17가지 천연유화제와의 유화력 비교시 biosurfactant의 유화력은 중간 수준인 것으로 나타났다. Biosurfactant를 함유하는 로션 타입의 처방에서는 biosurfactant를 함유하지 않은 처방에 비해 유화안정성이 높게 나타났고 특히 고온안정성은 2배 정도 상승되는 효과를 보였다. 25 hydrocarbon-metabolizing microorganisms were isolated from activated sludges and stream waters. Among them, the bacterium which decreased the surface tension and increased the emulsification power of culture broth mostly was selected. This bacterium was demonstrated to be a strain of Acinetobacter calcoaceticus as a result of automatic identification process by the Vitek System. This strain was able to use the various carbon sources including water-soluble carbon sources, many hydrocarbons and vegetable oils for the cell growth and the biosurfactant production. Among them, ethanol was the most effective carbon source for the biosurfactant production at 2% concentration. As a nitrogen source, urea was effective and the biosurfactant production was higher at C/N ratio=6.8, a condition of nitrogen limitation to a certain extent. When compared to 17 natural emulsifiers on home and foreign market now, emulsification power of biosurfactant was in middle level. The emulsification stability of biosurfactant-including lotion formulation was higher than that in no biosurfactant formulation and especially increased twofold in 45℃ stability test.
김윤석,서상렬,한병철,김방수,조진수 대한내과학회 1996 대한내과학회지 Vol.50 No.4
Two cases of vitamin B12 deficiency megaloblastic anemia are presented and literature for the cases are briefly reviewed. The first case is a 54-year-old man who was diagnosed as gastric ulcer perforation, and subtotal gastrectomy with gastrojejunostamy were performed 10 years ago. The second case is a 78-year-old man complicated with intrinsic factor deficiendcy and atrophic gastritis. Both cases were admitted to our hospital because of anemic symptoms such as dizziness, headache, tinnitus, general weakness, and appetite loss. Peripheral blood revealed thrombocytopenia, macrocytic anemia with anisocytosis and hypersegmented neutrophil, and bone marrow findings were decreased M:E ratio and increased cellularity. Schilling test revealed the excretion of 0.51% and 1.7% in 24hr urine collection (control 7%) respectly, and serum vitamin B12 level was decreased markedly. After treatment of parenteral vitamin B12, their clinical symptoms and hematologic findings improved progressively and they have been followed up on outpatient basis with maintenance therapy of vitamin B12.