들깨잎 폴리페놀 산화효소의 pH 및 온도에 의한 영향

김유경,김안근,Kim, Yoo-Kyung,Kim, An-Keun 대한약학회 2004 약학회지 Vol.48 No.6

Polyphenol oxidase-catalyzed oxidation of substrates (t-butylcatechol, 4-methylcatechol, chlorogenic acid, caffeic acid and pyrocatechol) were performed in the Ph range 4~8. Co ncentrations of substrate's major oxidation products were monitored by high performance liquid chromatograph. The nature and amounts of products formed were highly pH dependent. They also were ifluenced by kinds of substrates. Major oxidation product of 4-methylcatechol appeared the maxium value at pH 5, them of chlorogenic acid, caffeic acid and pyrocatechol at pH 6.0 and that of t-butylcatechol at pH 5~7. Time-dependent PPO activity was determined at $4^{\circ}C\;and\;30^{\circ}C$. PPO extracted by phosphate buffer containing triton X-114 (t-PPO) was more stable than PPO by phosphate buffer (b-PPO). The result of electrophoresis, at first PPO was showed only a band at 48 kd. After 1~3 days a partial degrade band was appeared in b-PPO and three partial degrade bands in t-PPO. No activity band was appeared in PPOs at $30^{\circ}C$ and b-PPO at $4^{\circ}C$ after 4 days. And a band (37 kDa) in t-PPO was remained finally and disappered. PPO from Perillae leaves has two activity bands at 48 and 37 kDa in previous paper. It was supposed that PPO in the leaves of Perilla frutescens was a protein having one molecular weight as 48 kDa. And 37 kDa protein, relatively proteolysis-resistant, was a proteolyzed form of a major form.

식품 섭취와 인지기능장애와의 관계: 체계적 문헌고찰 및 메타분석

김유경,김경희,Kim, Yoo-Gyeong,Kim, Kyung-Hee 한국식생활문화학회 2020 韓國食生活文化學會誌 Vol.35 No.6

This systematic review and meta-analysis aimed to determine whether food intake is effective in preventing diseases related to cognitive impairment. We searched English databases namely MEDLINE, PubMed and ScienceDirect from 2000 to May 2020, and Korean databases namely RISS, KISS, and DBPIA from 1990 to May 2020. We divided the data into 15 groups using the food group classification of the Korean Nutrition Society (KNS). The effect size (Cohen's d) was estimated using a random-effect model, and a 95% confidence interval was calculated for each study. We included 17 cross-sectional studies and 7 cohort studies which involved 45,115 participants. As a result of analyzing the subgroups in the Asian population of both sexes, it was observed that grain intake has a protective effect against cognitive impairment. For females, pulses and fish also have a protective role against cognitive impairment. In the case of seaweed, a negative relationship was found with a moderate protective effect against cognitive impairment (Cohen's d:-0.533, 95% CI: -0.939, -0.126; p=0.010) in Korean studies. Dairy products are associated with an increased risk of cognitive impairment in the American and European population but drinking alcohol is associated with a lower impairment risk. These results provide a basis for formulating the dietary guidelines for preventing dementia for each country.

국내 브랜드 커뮤니케이션 연구의 주제와 접근방법에 대한 메타분석

김유경(Kim, Yoo-kyung),강형구(Khang, Hyoung-koo),최일도(Choi, Il-do) 한국광고홍보학회 2008 광고연구 Vol.0 No.81

제주도 한우 집단에서 Galactose mutarotase (GALM) 유전자의 삽입/결실 다형성과 도체형질의 연관성 분석

김유경(Yoo-Kyung Kim),이종안(Jongan Lee),신상민(Sang-Min Shin),고응규(Yeoung-Gyu Ko),유지현(Ji-Hyun Yoo),원미영(Miyoung Won) 한국생명과학회 2023 생명과학회지 Vol.33 No.12

이 연구는 제주도 한우집단에서 galactose mutarotase (GALM) 유전자형과 도체형질의 연관성을 시험하였다. GALM 유전자형은 3’-비해독부위(3’-UTR)의 14-bp (5’-GGTCTAATGACCAG-3’) 삽입/결실 다형성을 이용하였다. 한우 비육우 집단에서 GALM 유전자의 세 가지 유전자형(LL, LS, SS)이 모두 관찰되었다. 연관성 분석결과는 근내지방의 함량과 밀접한 상관을 보이는 육질등급과 근내지방도의 수준과, 등지방두께의 수준이 유전자형에 따른 유의적인 차이를 나타내었다(p<0.05). 동형접합인 SS 유전자형을 보유한 도체에서 LL 또는 LS 유전자형인 도체에 비해 근내지방 함량 수준은 더 높고, 등지방두께도 더 얇은 수준을 보였다. 반면, 도체중, 등심단면적, 육색, 지방색 등은 GALM 유전자형에 따른 유의적인 차이는 없었다(p>0.05). 3’-UTR에서 14-bp 절편의 결실은 RNA의 2차 구조의 변형과 RNA-결합 단백질, microRNA와의 결합능력에 대한 방해를 통해 유전자 발현에 영향을 줄 수 있는 것으로 예측되었다. GALM 유전자의 3’-UTR 영역에서 14-bp 삽입/결실 다형성에 대한 이번 연구결과는 소에서 근육과 등지방 조직에서 galactose 대사에 의한 지방 축적을 통해 성장형질, 도체형질에 영향을 주는 것으로 판단된다. This study examined the associations between the genotypes of the galactose mutarotase (GALM) gene and carcass traits in the Hanwoo population of Jeju Island, South Korea. The GALM genotypes were determined by the 14-bp (5’-GGTCTAATGACCAG-3’) insertion/deletion (InDel) polymorphisms of the 3′-untranslated region (UTR). All three genotypes (LL, LS, and SS) were found in the Hanwoo steer population. The association analysis showed significant associations between genotypes and several carcass traits, including traits related to intramuscular fat content, such as meat quality, marbling score, and backfat thickness (p<0.05). Animals harboring the SS genotype showed not only higher levels of intramuscular fat content but also lower levels of backfat thickness than animals harboring the LL and LS genotypes. On the other hand, no significant associations were found between the GALM genotypes and carcass weight, eye muscle area, meat color, or fat color (p>0.05). Deleting the 14-bp segment in the 3′-UTR resulted in the modification of the secondary structure of RNA and appeared to affect gene expression by interfering with the binding ability of GALM mRNA with RNA-binding proteins and microRNAs. These results suggest that the 14-bp InDel polymorphism in the 3′-UTR region of the GALM gene affects cattle growth traits and carcass quality through galactose metabolism–mediated fat accumulation in muscle and backfat tissues.

유인헬리콥터와 무인기간 복합운용체계의 발전방향

김유경(Yoo Kyung Kim),이종훈(Jonghoon Lee) 항공우주시스템공학회 2019 항공우주시스템공학회 학술대회 발표집 Vol.2019 No.5

은염과 디지털 도구에 따른 조명비 교육의 실제 적용에 관한 연구

김유경 ( Yoo Kyung Kim ) 한국사진학회 2010 AURA Vol.0 No.22

Photographers deal with light in the limited area of visible ray and the difference in techniques to treat the given light makes difference in works of them. They sometimes have to determine the directions, angles, colors, and quality of light. Also, they know there is lighting ratio in any light that reaches subjects in any situation. The most difficult part of taking pictures in digital environment is that the rule of lighting ratio appled when using silver halide(film) to create the desirable mood is not adequate. Thus, I reviewed a thesis on this part as a preliminary study and found out that when the ratio of highlight and shadow are 1:1, 1:2, 1:4 the results of silver halide were nearly similar with those of digital but the depth of shadow of digital turned out to be opposite in case of 1:8, 1:16, 1:32. In case of digital, I could get results of lighting ratio learned in silver halide only when l took pictures with sequentially reduced light intensity of fill light so that it might be less than the value of lighting ratio indicated on exposure meter, comparing to silver halide. Therefore, this study intends to review the phenomenon and value of education appeared when the results of preliminary study were applied to lighting education. The education was implemented, divided into two groups; one group that experienced the results from the process of comparison experiment of silver halide and digital in real education were different and the other group that didn`t. In addition, I intended to understand the difference in representation properties of silver halide and digital and present the necessity of differentiated and proper approach toward lighting ratio education by analyzing results through questionnaire survey.

인터넷 광고의 내용분석을 통한 국가간 비교 연구

김유경(Yoo-Kyung Kim),김은희(Eun-Hee Kim) 한국방송학회 2001 한국방송학보 Vol.15 No.3

얼음두께를 이용한 결빙시험조건의 간접 확인기법

김유경(Yoo Kyung Kim),박남은(Nameun Park),최기오(Gio Choi) 한국항공우주학회 2018 韓國航空宇宙學會誌 Vol.46 No.11

회전익기의 결빙운용능력이 요구될 경우 자연결빙 비행시험전 풍동시험과 인공결빙시험을 선행하면 개발기간을 단축시킬수 있다. 한국형기동헬기의 경우 인공결빙 시험을 수행하여 각 시험조건에서 방 · 제빙기능의 정상작동을 확인 후 자연결빙시험을 수행하였다. 인공결빙시험에서 시험조건인 액적량(LWC)과 액적 평균직경(MVD)을 확인하기 위해 정밀센서인 구름기상정보관측기(CCP)를 사용하는데 시험환경, 장비오차등의 이유로 측정치의 불확실성이 존재할 수 있어 시험조건 확인이 다방면으로 필요하다. 동체에 착빙된 얼음두께로 부터 LWC를 계산하여 측정값과 비교한 결과, 시험조건을 간접적으로 검증해 볼 수 있는 효과적인 방법임을 확인하였다. Artificial icing test and wind tunnel test can be performed to reduce the development period when a rotorcraft is required operation under icing situations. Artificial icing test of the KUH(Korean Utility Helicopter) was performed in advance to verify anti-icing and de-icing performance before natural icing test. Although high-precision sensor, the CCP(Cloud Combination Probe) is used to measure icing test condition parameters such as LWC(Liquid Water Content) and MVD(Median Volume Diameter), the measured values need to be verified in various methods due to the possibility of uncertainties which are the test atmosphere environment, sensor errors, and etc. The calculated LWC from the ice thickness cumulated on the fuselage of the KUH is compared to the measured value by CCP, and the results show the effective indirect method to check the test conditions.

국가 정체성의 보편적 인식 차원과 영향에 관한 연구

김유경 ( You Kyung Kim ),김유신 ( Yoo Shin Kim ),박성현 ( Sung Hyun Park ),이효복 ( Hyo Bok Lee ) 한국PR학회 2012 PR연구 Vol.16 No.2

In order to raise the value of national brand, defining national identity that is based on competitiveness and bond of sympathy, which creates national image, must be done beforehand. Thus, the research focuses on investigating Korean university students on national identity that can be universally applied. A dimension of national identity, which includes nation`s history and value, and cultural uniqueness, global citizenship, national heritage, value system, global leadership, and emotional attachment based on goals to be aimed, was able to be drawn by the research. In addition, such dimensions were applied to United States of America, France, Japan, China, Iran, and many other rival nations to verify validity and examined effect of national identity on nation`s growth. A result from the research provided basic foundation of the study on national identity that is commonly applicable to each nation. In other words, a methodology from this research will be a valuable guideline to any follow-up investigations that is to be done on relevant nations. Furthermore, a definition of managing national identity based on this study is to inform with consistency about nation`s core capability and thoughts. The two useful conclusions drawn from this research are following. One is to infuse publics with a feeling of expectancy to stimulate intention to purchase; another is that it induces inner members to unite together for development of a nation under one common goal regardless of individual`s interest. Hence, a result from this research shows dimensions of each nation compared to other countries and implications in promotion & focused administration that corresponds to a goal, nation`s growth.

제주도 큰발윗수염박쥐(Myotis macrodactylus)의 유전적 집단 구조와 계통 유연관계

김유경(Yoo-Kyung Kim),박수곤(Su-Gon Park),한상훈(Sang-Hoon Han),한상현(Sang-Hyun Han),오홍식(Hong-Shik Oh) 한국생명과학회 2016 생명과학회지 Vol.26 No.7

본 연구는 미토콘드리아 DNA (mtDNA) cytochrome B (CYTB)와 NADH dehydrogenase subunit 1 (ND1) 유전자 서열의 다형성을 근거로 제주도 큰발윗수염박쥐 집단의 유전적 집단 구조와 계통 유연관계를 조사하는 데 목적이 있다. 동아시아 박쥐에서 CYTB 유전자 haplotype은 14개의 haplotype들이 발견되었고, ND1은 9개의 haplotype들이 발견되었다. 집단별 haplotype의 분포는 지역-특이적인 양상을 보였다. ND1 haplotype 분석결과에서 제주도 집단은 4개의 haplotype을 나타내고, 한라산 소집단과 서부지역 소집단은 3개 haplotype을 나타내었으나, 동부지역 소집단에서는 제주도 전체에서 공통으로 발견되는 1개(Nd03)의 haplotype만 출현하였다. NJ tree에서 제주도 집단은 강원도 집단보다 일본 집단과 더 근연으로 확인되었다. 중국과 일본의 모계선조 계보 사이의 분화 시점은 0.789±0.063 MYBP으로 추정되었고, 제주도와 일본의 모계선조는 약 17만 년(0.168±0.013 MYBP) 전에 분리된 것으로 판단된다. 제주도 집단은 적어도 5만 년 이전에 이주한 것으로 보인다. 또한 ND1 haplotype 분석결과는 제주도 집단이 이주 후에도 지역 내에서의 적어도 2회 이상의 유전적 분화를 겪었다는 것을 보여주고 있다. 본 연구결과는 동아시아 큰발윗수염박쥐의 계통 유연관계를 이해하는 데 중요한 기초자료가 될 것이며, 향후 한반도의 남부와 중국, 러시아 등에서 시료 확보를 통해 집단 간 진화적 상관관계를 이해하는 데 필요한 설득력 있는 자료가 마련되어야 할 것이다. This study was carried out to reveal the genetic population structure of the Jeju Island population and the phylogenetic relationship of East Asian populations of the large-footed bat (Myotis macrodactylus) based on the genetic polymorphisms of mitochondrial cytochrome B (CYTB) and NADH dehydrogenase subunit 1 (ND1) gene sequences. A total of fourteen and nine haplotypes were found in the CYTB and ND1 sequences from East Asian bats, respectively. Haplotype distribution showed locality specific patterns. The results from ND1 haplotype analysis showed that the Jeju Island population has four haplotypes: the Mt. Halla and Western subpopulations have three ND1 haplotypes, but the Eastern subpopulation has just a single haplotype Nd03, which is commonly found on this island. The neighbor-joining (NJ) tree showed the closer relationship between Jeju Island and Japan rather than that between Jeju and Gangwon-do Province. The divergence time between the maternal ancestor lineages of Japanese and Chinese populations was estimated to be 0.789±0.063 MYBP. The secondary divergence between Jeju and Japanese bats was calculated about to be 0.168±0.013 MYBP. The Jeju population has immigrated to the island at least fifty thousand years ago. In addition, ND1 haplotype analysis suggested that the insular bats have experienced at least two further genetic differentiation events within this island. Consequently, these findings suggested that the results of this study may play a critical role in understanding the phylogenetic relationship among East Asian bat populations of M. macrodactylus. To prepare more explainable information on evolutionary correlation, analysis is still required to examine using expanded samples from China, Russia, and southern parts of the Korean Peninsula.