Polymerization chain reaction과 Southern hybridization을 이용한 Salmonella속 균의 신속한 검출

김원용,장영효,박경윤,김철중,신광순,박용하,Kim, Won-yong,Chang, Young-hyo,Park, Kyoung-yoon,Kim, Chul-joong,Shin, Kwang-soon,Park, Yong-ha 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.3

Salmonella species are the most prevalent etiologic agents of food-borne acute gastroenteritis. Direct isolation of bacteria from the contaminated food, stool and animal tissues has been used for the diagnosis of salmonellosis routinely. However, isolation of bacteria is time consuming work and not so highly sensitive. In recent years, improved methods of polymerization chain reaction(PCR) and probe hybridization technique have led to the developement of diagnostic assays which employ to detect various human and animal pathogenic bacteria. In this study, we have performed the polymerization chain reaction to detect Salmonella pullorum from tissues and stool samples of chickens with two specific primers, ST5 and ST8C. The target DNA fragment of PhoE gene was successfully amplified from liver, spleen, pancreas, heart, lung, ovary, oviduct and feces samples. The amplified DNA fragments were hybridized with Salmonella typhymurium TA3000 PhoE probe by Southern hybridization. The PCR to amplify the PhoE gene was highly rapid and sensitive method to detect Salmonella pullorum from tissues and stool samples.

가금에서 분리한 Salmonella속 균의 항균물질에 대한 감수성 및 plasmid profile

김원용,장영효,박경윤,김철중,신광순,박용하,Kim, Won-yong,Chang, Young-hyo,Park, Kyoung-yoon,Kim, Chul-joong,Shin, Kwang-soon,Park, Yong-ha 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.3

In this study, we aim to find the presence of virulence-related plasmid in Salmonella isolates from poultry, and the difference between S pullorum and S gallinarum on the plasmid profile and antibiotics resistance. We used seventeen isolates of Salmonella spp that were isolated from poultry. Thirteen isolates, S typhimurium(ST), S pullorum(SP) and S gallinarum(SG), contained virulence-related plasmids. These are 95Kd plasmid in ST and 85Kd plasmid in SP and SG. Three(1/4 of ST, 1/1 of SE, and 1/9 of SP) isolates have no detectable plasmids. The isolates of ST have relatively variable plasmid profile but the isolates of S pullorum except No 12(additional 3.0Kb plasmid) have common 85K6, 8.1Kb, 4.0Kb and 2.3Kb plasmid and two of three isolates of S gallinarum have common 85Kb, 4.0Kb and 2.3Kb plasmid but the rest has only 85Kb plasmid. Interestingly, all of the isolates of SP have 8.1Kb plasmid, and same size of plasmid is also found in one of ST isolates. All of the isolates have the resistance to penicillin, chloramphenicol, rifampicin, streptomycin, sulfamethazine and some isolates show the resistance to ampicillin and tetracycline. There is no relatedness between plasmid profile and antibiotics resistance and no differences between SP and SG in antibiotics resistance. Therefore further differentiation of each isolates by restriction enzyme assay and, if possible, charaterization of each plasmid, especially, 8.1Kb plasmid in SP and ST, may be necessary.

Flavin Analogue 가 백서 간장내 포도당 및 지방 대사에 미치는 영향

김원용,황석귀,김윤수 ( Won Yong Kim,Suk Kuy Whang,Yoon Soo Kim ) 생화학분자생물학회 1978 BMB Reports Vol.11 No.1

Lambooy (1961) has shown that, while several analogues of riboflavin are able to replace the vitamins in the metabolism of some microorganism, 7-ethyl-8-methyl flavin is able to serve as the sole flavin in the metabolism of the rat. This flavin is able to serve as a completely adequate replacement for riboflavin in the metabolism of Wistar (Lambooy, 1961) and Sprague-Dawley rats (Yoon S. Kim et al, 1966) in terms of growth, survival, optimal physical appearance, and efficient utilization of food. Administration of 7-ethyl-8-methyl flavin to weanling rats as their only source of flavin permits a normal growth with a concomitant rapid and extensive loss of succinic dehydrogenase activity (SDH) from the liver, heart and kidney to within 24, 48, and 67% of the normal level, respectively, on day 42 following initiation of a 7-ethyl-8-methyl flavin diet. This result was essentially identical with that previously reported (Y.S. Kim and Lambooy, 1967). The purpose of the experiments described here is to study whether the decreased SDH activity in the tissue of rats is related to the metabolism of glucose and de novo biosynthesis of lipid from glucose in the liver of rats when D-glucose-^(14)C(U) is administered intraperitoneally to the rat maintaining SDH activity from liver, heart and kidney at 24, 48 and 67% of the normal level. This is also to study whether the total triglyceride and the total lipid content in the liver of rats is influenced by the loss of SDH activity in the liver of rats which have received 7-ethyl8-methyl flavin. The present study shows that there wa.s no significant difference in the total amount of ^(14)CO₂ expired from D-glucose-^(14)C(U) through the respiration of rats which had received 7-ethyl-8-methyl flavin compared to control rats which received riboflavin, indicating that the metabolic rate of glucose in the tissues of rat receiving 7-ethyl-8-methyl flavin is similar to that of the control rats. However, compared to the control level only 50% of ^(14)C total lipids were incorporated by de novo biosynthesis from D-glucose-^(14)C(U) in the liver of rats receiving 7-ethyl-8-methyl flavin but the total content of triglyceride and total lipids in the liver of rats receiving 7-ethyl-8-methyl flavin were increased by 29 and 30% over the normal level. This may be due to the increased of lipid transport from the peripheral adipose tissue due to the decreased SDH activity in the liver of rats.

국내에서 분리한 encephalomyocarditis virus의 성상

김원용,마점술,김철중,강신영,최재윤,하용공,Kim, Won-yong,Mah, Jum-sool,Kim, Chul-joong,Kang, Shien-young,Choi, Jae-yoon,Ha, Yong-kong 대한수의학회 1992 大韓獸醫學會誌 Vol.32 No.4

We have investigated the characteristics of encephalomyocarditis(EMC) virus isolated in Korea. The CPE, buoyant density, polypeptide profile and the size of RNA of EMC virus were examined. The granulation, pyknosis and necrosis were observed from 30 to 48 hour's post inoculation of the virus into baby hamster kidney and lung cells. The buoyant density was 1.30 and $1.35g/m{\ell}$. Three different polypeptides, 26Kd, 32Kd, and 34Kd in size, were observed and the size of viral RNA was 7.7Kb.

Flayin Analogue가 백서 간장내 포도당 및 지방 대사에 미치는 영향

김원용,황석귀,김윤수,Kim, Won-Yong,Whang, Suk-Kuy,Kim, Yoon-Soo 생화학분자생물학회 1978 한국생화학회지 Vol.11 No.1

Lambooy (1961) has shown that, while several analogues of riboflavin are able to replace the vitamins in the metabolism of some microorganism, 7-ethyl-8-methyl flavin is able to serve as the sale flavin in the metabolism of the rat. This flavin is able to serve as a completely adequate replacement for riboflavin in the metabolism of Wistar (Lambooy, 1961) and Sprague-Dawley rats (Yoon S. Kim et al, 1966) in terms of growth, survival, optimal physical appearance, and efficient utilization of food. Administration of 7-ethyl-8-methyl flavin to weanling rats as their only source of flavin permits a normal growth with a concomitant rapid and extensive loss of succinic dehydrogenase activity (SDH) from the liver, heart and kidney to within 24, 48, and 67% of the normal level, respectively, on day 42 following initiation of a 7-ethyl-8-methyl flavin diet. This result was essentially identical with that previously reported (Y.S. Kim and Lambooy, 1967). The purpose of the experiments described here is to study whether the decreased SDH activity in the tissue of rats is related to the metabolism of glucose and de novo biosynthesis of lipid from glucose in the liver of rats when D-glucose-$^{14}C$(U) is administered intraperitoneally to the rat maintaining SDH activity from liver, heart and kidney at 24, 48 and 67% of the normal level. This is also to study whether the total triglyceride and the total lipid content in the liver of rats is influenced by the loss of SDH activity in the liver of rats which have received 7-ethyl-8-methyl flavin. The present study shows that there was no significant difference in the total amount of $^{14}CO_2$ expired from D-glucose-$^{14}C$(U) through the respiration of rats which had received 7-ethyl-8-methyl flavin compared to control rats which received riboflavin, indicating that the matabolic rate of glucose in the tissues of rat receiving 7-ethyl-8-methyl flavin is similar to that of the control rats. However, compared to the control level only 50% of $^{14}C$ total lipids were incorporated by de novo biosynthesis from D-glucose-$^{14}C$(U) in the liver of rats receiving 7-ethyl-8-methyl flavin but the total content of triglyceride and total lipids in the liver of rats receiving 7-ethyl-8-methyl flavin were increased by 29 and 30% over the normal level. This may be due to the increased of lipid transport from the peripheral adipose tissue due to the decreased SDH activity in the liver of rats. 7-Ethyl-8-methyl flavin이 SDH 효소 활성에 미치는 영향에 대하여 Kim and Lambooy (1967)에 의해 보고된 바 있다. 즉 이 flavin 투여후 30일에 쥐 간장에서 SDH 효소 활성은 정상군의 해당조직 효소 활성치를 100%로 할때 23%로 떨어져 100일 까지 계속 20% 내외로 유지하여, 심장에서는 투여 30일에 64% 50일에 43% 100일에서 31% 내외였고, 신장에서는 30일에 72% 50일에 68% 100일에 56% 내외를 유지한다고 보고 하였다. 저자는 본 실험을 시작하기전에 Kim and Lambooy (1967)방법에 따라 쥐에 이 flavin을 투여한 후 42일에 SDH 효소 활성을 측정한바 간장에서 24% 심장에서 48% 신장에서 76%로 떨어졌으나, 그 쥐의 성장, 체중의 증가, 식이 섭취량 및 외모등이 riboflavin 투여군과 동일한 사실을 재확인 하였으며 이런 SDH 효소 활성을 유지하는 쥐에다 D-glucose $^{14}C$ (U)를 마리당 $55{\times}10^5$ dpm 씩 복강내 주입하고 이 쥐를 metabolic cage에 넣어 호흡을 통하여 나오는 총 $^{14}CO_2$의 배설량을 methylcellosolve:ethanolamine 용액 (2:1)에 포착시켜 정량한바 riboflavin 투여군에서는 평균 $257192{\pm}8630\;cpm$/3hrs/gm of body wt. 7-ethhyl-8-methyl flavin 군은 평균 $255426{\pm}1160\;cpm$/3hrs/gm of body wt. 로 두군의 총 $^{14}CO_2$ 배설량은 서로 같았다. 7-Ethyl-8-methyl flavin에 의하여 SDH 활성이 떨어졌음에도 총 $^{14}CO_2$ 배설량에 하등의 변동이 없는 이 두군에서 성장 및 체중증가, 식이 섭취량, 외모 등의 동일성으로 미루어보아 체내에 전체적으로 영향을 주는 대사장애는 없는 것으로 사려되나, 투여한 D-glucose $^{14}C$ (U)로부터 간장내 지방 de novo 생합성은 평균 $13080{\pm}420$ cpm per gm에서 $6595{\pm}400$ cpm per gm 으로 7-ethyl-8-methyl flavin 투여군에서 50%나 현저히 감소하였다. 간장지방내 $^{14}C$ 는 모두투여한 $^{14}C$ (U)로부터 합성된 것임으로 이 결과는 간장 조직내에서 SD H 활성 저하가 지방 de novo 생합성과 밀접한 관계가 있음을 시사해준다. 즉 SD H 활성이 떨어지면 Krebs cycle 저하를 초래하고 이것은 glucose로 부터 지방의 de novo 생합성을 떨어뜨리는 결과로 사려된다. 간장내 총 triglyceride 함량은 정상군에서 평균 $1.93{\pm}0.083gm%$이나 7-ethyl-8-methyl flavin 투여군에서는 $2.49{\pm}0.085gm%$로 29%가 증가하였으며 간장내 총지방함량 역시 정상군에서 평균 $4.53{\pm}0.299gm%$인데 비해 7-ethyl-8-methyl flavin 투여군에서는 $5.90{\pm}0.157gm%$로 정상군보다 30% 증가하였다. 이상 결과로 미루어 보아 7-ethyl-8-methyl flavin 투여로 간장에서 SDH 합성이 24%로 떨어지면 간장내 지방의 de novo 생합성은 50% 감소되나 총 triglyceride 함량은 정상군보다 29%, 총지방함량은 30% 증가하였다. 이는 아마도 체내 SDH 활성저하가 말초 지방조직으로부터 간장조직으로 지방산의 이동을 증가시키는 결과로 사려된다.

전곡리 (全谷里) 아슐리안 양면핵석기문화 (兩面核石器文化) 예보 (豫報)

김원용,정영화 ( Won Yong Kim,Young Wha Chung ) 진단학회 1979 진단학보 Vol.- No.46-47

화순출토 세문경 (和順出土 細文鏡)

김원용,Kim, Won-Yong 국립문화재연구원 1972 헤리티지:역사와 과학 Vol.6 No.-

신라 · 가야고분의 제문제 - 그 발생과 발전

김원용,김기웅,이은창,진홍섭 ( Won Yong Kim,Ki Ung Kim,En Chang Yi,Hong Sup Chin ) 일지사 1976 韓國學報 Vol.2 No.1

사당동 신라토기요지 조사 약보 (舍堂洞 新羅土器窯址 調査 略報)

김원용,이종선,Kim, Won-Yong,Lee, Jong-Seon 국립문화재연구원 1977 헤리티지:역사와 과학 Vol.11 No.-

올바른 문화재관리(文化財管理)를 위(爲)하여

김원용,Kim, Won-Yong 국립문화재연구원 1965 헤리티지:역사와 과학 Vol.1 No.-