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Polymerization chain reaction과 Southern hybridization을 이용한 Salmonella속 균의 신속한 검출
김원용,장영효,박경윤,김철중,신광순,박용하,Kim, Won-yong,Chang, Young-hyo,Park, Kyoung-yoon,Kim, Chul-joong,Shin, Kwang-soon,Park, Yong-ha 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.3
Salmonella species are the most prevalent etiologic agents of food-borne acute gastroenteritis. Direct isolation of bacteria from the contaminated food, stool and animal tissues has been used for the diagnosis of salmonellosis routinely. However, isolation of bacteria is time consuming work and not so highly sensitive. In recent years, improved methods of polymerization chain reaction(PCR) and probe hybridization technique have led to the developement of diagnostic assays which employ to detect various human and animal pathogenic bacteria. In this study, we have performed the polymerization chain reaction to detect Salmonella pullorum from tissues and stool samples of chickens with two specific primers, ST5 and ST8C. The target DNA fragment of PhoE gene was successfully amplified from liver, spleen, pancreas, heart, lung, ovary, oviduct and feces samples. The amplified DNA fragments were hybridized with Salmonella typhymurium TA3000 PhoE probe by Southern hybridization. The PCR to amplify the PhoE gene was highly rapid and sensitive method to detect Salmonella pullorum from tissues and stool samples.
국내에서 분리한 encephalomyocarditis virus의 성상
김원용,마점술,김철중,강신영,최재윤,하용공,Kim, Won-yong,Mah, Jum-sool,Kim, Chul-joong,Kang, Shien-young,Choi, Jae-yoon,Ha, Yong-kong 대한수의학회 1992 大韓獸醫學會誌 Vol.32 No.4
We have investigated the characteristics of encephalomyocarditis(EMC) virus isolated in Korea. The CPE, buoyant density, polypeptide profile and the size of RNA of EMC virus were examined. The granulation, pyknosis and necrosis were observed from 30 to 48 hour's post inoculation of the virus into baby hamster kidney and lung cells. The buoyant density was 1.30 and $1.35g/m{\ell}$. Three different polypeptides, 26Kd, 32Kd, and 34Kd in size, were observed and the size of viral RNA was 7.7Kb.
김원용,이영상,김광배,강인찬 ( Won Yong Kim,Young Sang Lee,Gwang Bae Kim,In Chan Kang ) 한국주조공학회 1990 한국주조공학회지 Vol.10 No.5
N/A The application of this new design approach called fracture mechanics allow one to determine the maximum allowable stress from the knowledge of the largest expected flow size and the plane strain fracture toughness of a material. In this study we examined the relation between retained austenite, mechanical property and fracture toughness accompanied by austempering heat treatment. Fracture toughness values and retained austenite volume were higher with the ADI (austempered ductile iron) which were austempered at 380℃ than austempered at 320℃. Additionally, fracture toughness values were increased for 1∼2hour austempering time but it was slowly decreased for 5hour ADI maintaining the predominant fracture toughness(Kic:83㎫√m) is obtained following condition, namely, austempering temperature and time (380℃ and 1hour).
가금에서 분리한 Salmonella속 균의 항균물질에 대한 감수성 및 plasmid profile
김원용,장영효,박경윤,김철중,신광순,박용하,Kim, Won-yong,Chang, Young-hyo,Park, Kyoung-yoon,Kim, Chul-joong,Shin, Kwang-soon,Park, Yong-ha 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.3
In this study, we aim to find the presence of virulence-related plasmid in Salmonella isolates from poultry, and the difference between S pullorum and S gallinarum on the plasmid profile and antibiotics resistance. We used seventeen isolates of Salmonella spp that were isolated from poultry. Thirteen isolates, S typhimurium(ST), S pullorum(SP) and S gallinarum(SG), contained virulence-related plasmids. These are 95Kd plasmid in ST and 85Kd plasmid in SP and SG. Three(1/4 of ST, 1/1 of SE, and 1/9 of SP) isolates have no detectable plasmids. The isolates of ST have relatively variable plasmid profile but the isolates of S pullorum except No 12(additional 3.0Kb plasmid) have common 85K6, 8.1Kb, 4.0Kb and 2.3Kb plasmid and two of three isolates of S gallinarum have common 85Kb, 4.0Kb and 2.3Kb plasmid but the rest has only 85Kb plasmid. Interestingly, all of the isolates of SP have 8.1Kb plasmid, and same size of plasmid is also found in one of ST isolates. All of the isolates have the resistance to penicillin, chloramphenicol, rifampicin, streptomycin, sulfamethazine and some isolates show the resistance to ampicillin and tetracycline. There is no relatedness between plasmid profile and antibiotics resistance and no differences between SP and SG in antibiotics resistance. Therefore further differentiation of each isolates by restriction enzyme assay and, if possible, charaterization of each plasmid, especially, 8.1Kb plasmid in SP and ST, may be necessary.
우황청심원의 고문헌기록 및 실험적 연구결과 분석을 통한 임상응용 확대의 필요성 고찰
오영택,오현묵,김서우,김원용,손창규,조정효,Oh, Young-Taeck,Oh, Hyeon-Muk,Kim, Seo-Woo,Kim, Won-Yong,Son, Chang-Gue,Cho, Jung-Hyo 대전대학교 한의학연구소 2017 혜화의학회지 Vol.26 No.1
Objectives: The aim of this study is (1) to investigate the historic changes and pharmacological efficacies of Woohwangchungsim-won and (2) to discuss the necessities for Woohwangchungsim-won's clinical applications. Methods: This study was performed through (1) investigating the ancient literature records related with Woohwangchungsim-won and analyzing Woohwangchungsim-won's composition, dosage and indications, (2) searching articles about Woohwangchungsim-won on 10 major Korean web and 3 major foreign web article search engines and analyzing Woohwangchungsim-won's pharmacological efficacies and indications. Results: Woohwangchungsim-wom has been used for cerebrovascular diseases such as stroke and palpitation. Also, there are some ancient literature records of Woohwangchungsim-won's clinical applications in neuropsychiatric disorders such as depression and bipolar disorder. In addition, there have been a number of experimental studies which demonstrate Woohwangchungsim-won's neuroprotective effect on cerebral cortex and hippocampus injury. So, it is possible to infer that Woohwangchungsim-won can be used for the treatments of neuropsychiatric disorders associated with neuronal cell death in cerebral cortex and hippocampus. But there have been no or less experimental studies which demonstrate the pharmacological efficacy of Woohwangchungsim-won on such disease. Conclusion: It is necessary that further experimental studies which demonstrate Woohwangchungsim-won's pharmacological efficacy on neuropsychiatric disorders should be done and Woohwangchungsim-won's clinical applications should be expanded on the basis of those related experimental results.
김원용(Won-yong Kim),김종원(Jong-won Kim),김현준(Hyun-jun Kim),김경동(Kyoung-dong Kim),김도현(Do-hyun Kim),최대식(Dae-sik Choi),오형록(Hyoung-lok Oh),이준영(Jun-young Lee) 전력전자학회 2011 전력전자학술대회 논문집 Vol.2011 No.7
최근 하이브리드 및 전기자동차의 집약적인 발전과 함께 충전기술이 대두 되고 있다. 본 논문은 충전기능만을 고려한 단방향 충전시스템에서 양방향 배터리 충/방전 시스템에 대하여 서술한다. 양방향 시스템은 주차 된 차량의 배터리를 저장 매체로 활용하여 V2G(Vehicle to Grid)기술로 활용할 수 있어 잉여전력에 대해서 계통으로 전력을 전송할 수 있다. 용량은 가정용으로써 3.3㎾ 탑재형 단상 양방향 충/방전기에 대한 시뮬레이션과 실험을 통해 결과를 도출했다.