양극산화 처리된 타이타늄 표면에서 골형성 유전자 발현

김원석,김영석,전성배,전상호,이의석,장현석,권종진,임재석,Kim, Won-Seok,Kim, Young-Seok,Jeon, Seong-Bae,Jun, Sang-Ho,Lee, Eui-Suk,Jang, Hyon-Seok,Kwon, Jong-Jin,Rim, Jae-Suk 대한악안면성형재건외과학회 2012 Maxillofacial Plastic Reconstructive Surgery Vol.34 No.2

Purpose: The purpose of this study was to evaluate the expression of osteogenic genes associated with bone regeneration on anodizing titanium surface. Methods: $20{\times}20{\times}1$ (mm) commercially pure titanium plate was made, one group was pure titanium, second group was punched, and last group was punched and anodized by electrochemical method. Through the osteogenic cell culture model, the expression of extracellular matrix proteins, such as bone morphogenetic protein-2, bone sialoprotein, aggrecan, osteocalcin, Alkaline phosphatase, collagen I had been evaluated by Real-time polymerase chain reaction, and the morphology of growing cells was evaluated by scanning electron microscopy. Results: The attachment of mesenchymal stem cell was even and well-oriented on all Ti surfaces. The osteogene expression was increased on punching groups but, decreased on anodizing surfaces in 3 week samples. Conclusion: Punched anodizing Ti has possibility be using as a dental implant material, but further in vivo study would be needed.

염분 노출에 따른 Chironomus riparius의 성장지연과 Heat shock protein 70 유전자 발현

김원석,임병현,홍철,최승원,박기연,곽인실,Kim, Won-Seok,Im, Byeong-Hyeon,Hong, Cheol,Choi, Seung-Won,Park, Kiyun,Kwak, Ihn-Sil 한국하천호수학회 2017 생태와 환경 Vol.50 No.3

We investigate that the impact of freshwater organism exposed to the salinity environment by the frequent rainfall following climate change. To evaluate the stress response following salinity exposure, we assessed the survival rate, molting success rate, the developmental period and mouthpart deformities in Chironomus riparius. In addition, we measured the molecular responses of biomarker gene, gene expression of heat shock protein 70 (HSP70) in C. riparius exposed to salinity after 96 hour. The C. riparius survival rates were showed on time dependent manner and not observed survival organisms above 15 psu at day 4. The pupation and emergence of C. riparius were not seen above 15 psu, and the molting success rate was less than 20% at 10 psu. The developmental retardation of C. riparius was well observed in the pupation and emergence period and was delayed by 4 days at 10 psu compared to the control and 5 psu. The mouthpart deformities after salinity exposure at 96 or 72 hour were observed at 10 psu and 15 psu. The expression of C. riparius HSP70 level was significantly increased exposure to 5 psu and 10 psu. Thus, salinity has been caused to be various ecotoxicological and molecular stress responses on freshwater organisms similar to harmful substances such as EDCs and so on. 기후변화로 잦아진 강우에 의해 염분 환경에 유입되는 담수생물의 영향을 파악해 보고자 하였다. 담수의 오염지표생물인 깔따구과의 실내 실험종인 C. riparius를 대상으로 염분 노출에 따른 스트레스 반응으로 생존율과 탈피율, 성장기간, 하순기절 기형을 분석하였고, 분자생물학적으로 스트레스 분자마커로 연구가 이루어진 HSP70 유전자 발현을 측정하였다. C. riparius의 생존율은 노출시간에 따라 감소하였으며 15 psu에서 4일이 경과하면 생존개체가 관찰되지 않았다. C. riparius의 pupation과 emergence는 15 psu 이상에서는 보이지 않았고 탈피의 성공은 10 psu에서 20% 이하였다. 또한 C. riparius의 성장지연은 pupation과 emergence 기간에서 잘 반영되어, 대조군과 5 psu에 비해 10 psu에서 4일이 늦어졌다. 염분 노출 96시간 후 관찰한 C. riparius 하순기절 형태 이상은 10 psu와 15 psu에서 소수 관찰되었다. C. riparius HSP70 유전자 발현은 대조군에 비해 5, 10 psu에서 약 2.5~4배 이상 발현량이 크게 증가하였다. 따라서 염분은 담수생물에게 매우 큰 스트레스를 제공하는 원인물질로 유해물질과 유사한 생태독성학적인 반응과 분자지표 반응을 보여주었다.

건설안전실무 - 교량 공법별 안전점검

김원석,Kim, Won-Seok 대한산업안전협회 2011 안전기술 Vol.168 No.-

오존(O₃) 노출에 의한 조각깔따구(Glyptotendipes tokunagai)의 체색 변화 및 heat shock protein 70 발현 변화

김원석,최보형,김문경,채선하,곽인실,Kim, Won-Seok,Choi, Bohyung,Kim, Moon-Kyung,Chae, Seon Ha,Kwak, Ihn-Sil 한국하천호수학회 2020 생태와 환경 Vol.53 No.4

오존은 수돗물 정수장에서 이용되는 소독 물질로 미세오염 물질들을 비롯해서 박테리아나 병원성 미생물체를 효과적으로 제거하는 것으로 많은 연구가 보고되어 있다. 본 연구에서는 실내 사육 중인 붉은 체색을 지닌 Glyptotendipes tokunagai를 대상으로 서로 다른 농도의 오존 노출에 따른 영향을 파악하기 위해 치사율, 체색 변화와 heat shock protein 70 (HSP70) 유전자 발현을 측정하였다. 오존에 노출된 G. tokunagai에서 농도-시간 의존적으로 치사율 증가가 관찰되었다. 또한 체색 변화는 오존 농도에 따라 붉은색의 체색이 체절마다 엷어지며 탈색되고 경직되는 현상이 보였다. HSP70 유전자 발현은 저농도인 0.2~0.5 ppm에서 노출 10분과 20분에 유의한 수준으로 높게 나타났으나(P<0.05), 30분 노출 후에는 발현량이 감소하는 경향을 보였다. 생리적으로 저산소층에 대해 적응능력이 뛰어난 깔따구 경우에도 오존은 매우 강력한 치사 효과를 유발하여 30분 노출 후 경직과 헤모글로빈 파괴로 인한 탈색이 유발되는 것을 보여주었다. 따라서 본 결과는 수돗물 정수장에서 병원성 미생물을 제거하는 데 사용되는 오존이 수생물에 주는 영향성을 파악하는 기초자료로서 활용될 수 있을 것이다. Ozone (O₃) is a general disinfectant to remove micro-pollutants in water treatment system. Previous studies have reported effect of ozone to bacteria and pathogens removal, but its effect to the relatively large organisms has little known. In this study, we investigated potential effects of ozone toxicity to the non-bite midge larvae (Glyptotendipes tokunagai) with accumulate mortality, coloration change and expression of heat shock protein 70 (HSP70). The accumulate mortality rate of G. tokunagai increased in a dose-time dependent manner and the highest mortality rate was observed to 75% at 30 minute of exposure duration with 2.0 ppm of ozone concentration. Exposure to ozone was a factor increasing body color of the larvae. The tendency of HSP70 mRNA expression showed up-regulation in ozone exposure at 20 minute. After that time, the expression of HSP70 in exposed group decreased to a similar level of control group. Our results clearly showed that ozone toxicity affects physical and molecular activity of G. tokunagai, implying the potential hazardous of ozone in the aquatic ecosystem including macroinvertebrates.

불포화 암반 파쇄대를 통한 핵종 이동

김원석(Won Seok Kim),김정진(Jungjin Kim),안진모(Jinmo Ahn),남성식(Seongsik Nam),엄우용(Wooyong Um) 大韓環境工學會 2015 대한환경공학회지 Vol.37 No.8

불포화대 암반 단열에서 방사성 핵종인 3H, 90Sr and 99Tc의 흡착실험이 진행되었다. 천층처분시설의 인공방벽을 통과해 누출된 방사성 핵종은 빗물이나 공극수에 의해 불포화대 암반 단열을 통하여 지하수로 도달하게 된다. 그러므로 처분장의 장기간 안전점검을 위해, 불포화대 암반 단열을 통한 방사성 핵종의 거동을 연구하는 것이 중요하다. 천층처분주변에서 채취된 불포화대 암반 단열 샘플을 이용하여 X-ray microtomography 분석을 수행하였고, 회분식 흡착실험을 이용하여 방사성핵종인 3H, 90Sr and 99Tc의 흡착실험이 진행되었다. 암반 단열의 충전물질로 불석광물 및 점토광물 존재 시 중흡착성 핵종인 90Sr의 흡착 분배계수 값이 충전물질이 존재하지 않을 때 보다 높게 나타내었다. 본 연구를 통해, 암반 단열 특성화 및 방사성 핵종의 흡착분배계수를 구했으며, 불포화대 암반 단열을 통한 핵종의 거동이 지연됨을 이해할 수 있었다. Adsorption experiments for radionuclides such as 3H, 90Sr and 99Tc were conducted using fractured rock collected in unsaturated zone. The released radionuclide through artificial barrier from the near surface repository can be transported by the flow of rainfall or pore water through fractures in unsaturated zone and reach to groundwater flow. Therefore, it is important to investigate transport behavior (retardation) of radionuclides through fractured rock for the safety assessment and long-term performance of repository. Fractured rock samples were collected and characterized by X-ray microtomography (XMT) analysis, which can be used to develop a more robust unsaturated fracture transport model. When fracture-filling materials are exist, distribution coefficient of 90Sr is higher than without fracture-filling materials. In this study, batch sorption distribution coefficient (Kd) of radionuclide was determined and used to increase our understanding of radionuclide retardtion through fracture-filling materials.

저소득층 장애인 주택 주거환경 및 에너지 효율 개선을 위한 개ㆍ보수 사례 연구

김원석(Kim, Won-Seok),이명주(Lee, Myoung-Ju) 대한건축학회 2015 대한건축학회논문집 Vol.31 No.1

This research is based on case studies of ten houses subjected to renovation in energy performance improvement for the physically disabled residents. The project is distinctive in its synchronized consideration for barrier-free design, home improvements, and energy performance at the same time, unlike the regular renovation projects for the disabled home owners. Among categories for the improvements, the furniture replacement and installation was ranked the highest. For the energy performance improvements, replacement of external windows was in the highest need. The total cost for the energy performance renovation was calculated average of 4,140,000 KRW. By categories, the highest was replacements for external windows, which required average of 1,240,000 KRW. Based on the analysis of reduced heating and cooling energy demands after renovation, the demands decreased average of 773.1 kWh/yr. Based on the analysis of reduced heating and cooling energy demands after renovation, The decreased demands in percentage averaging on 16.6%. The comparison analysis on construction costs show that every 10,000 KRW saves 1.90 kWh/yr of heating and cooling energy demands. However the decrease rate was not proportional to the construction cost. In order to magnify the effect and range of energy performance improvements, consideration on low-cost, high-efficiency planning and thorough analysis on the subjected house should be practiced from the beginning stage.

PFOS (perfluorooctane sulfonate) 노출에 따른 조간대 칠게(Macrophthalmus japonicus)의 생태독성학적 판정점 제시

김원석 ( Won-seok Kim ),박기연 ( Ki Yun Park ),차밀라니 ( Chamilani Nikapitiya ),곽인실 ( Ihn-sil Kwak ) 한국환경생물학회 2014 환경생물 : 환경생물학회지 Vol.32 No.4

PFOS (Perfluorooctane sulfonate) 노출에 따른 Macrophthalmusjaponicus의 생태독성학적 판정점을 탐색하고자 생존율, 부속지 탈락 수, 갑각 및 내장 색의 변화를 관찰하였다. 그 결과, PFOS 노출에 따른 생존율의 감소는 농도 의존적 패턴을 보여 상대적 고농도인 PFOS 30μg L-1에서 168시간 노출시 24%의 가장 낮은 생존율을 나타내었다. 부속지 탈락은 대조군에 비해 PFOS 노출군에서 탈락빈도가 증가하였다. 또한 대조군에서는 갑각색의 변화가 관찰되지 않았으나, PFOS 노출군에서는 백화현상이 농도의존적 패턴으로 관찰되었다. 내부 장기의색 선명도도 PFOS 노출군에서 대조군에 비해 변화가 나타났다. 이러한 결과는 해양 저서환경의 모니터링을 위한 생물적 주요한 정보를 제공해 줄 것이다. PFOS (perfluorooctane sulfonate) is one of the perflourinated organic compound, which persist as a residual compound in the coastal environments. Intertidal mud crab Macrophthalmus japonicus mainly inhabits in coastal and bay ecosystems in Indo-Pacific region including Korea and reflects to environmental changes. In the present study, M. japonicus were exposed to different concentrations of PFOS and various ecotoxicological end-points such as survival rate, elimination of appendages, changes of the crust and internal organ color changes were investigated. Interestingly, the PFOS exposure showed concentration-dependent decrease of survival rate. High PFOS exposure (30 μg L-1) showed a low survival rate of 24% at 168 hours. Further, in comparison with the controls, the rate of elimination of appendages was also considerably increased in a time dependent manner upon PFOS exposure. Notably, with progression of time, an increased exposure to PFOS, test species showed whitening effect in a concentration-dependent manner, whereas the crab crust color was unchanged in the control. In addition, change in internal organs color and their visibility (clarity) observed in PFOS exposed crabs compared to control. Taken together, we suggest, eco-toxicology end-points of M. japonicus exposed to PFOS gave important biological information which could be useful to identify toxic contamination in the marine benthic environments.

전자 수용체가 BTEX, MTBE로 오염된 토양의 혐기성 자연정화에 미치는 영향

김원석 ( Won Seok Kim ),김지은 ( Ji Eun Kim ),백지혜 ( Ji Hye Baek ),상병인 ( Byoung In Sang ) 한국물환경학회 2005 한국물환경학회지 Vol.21 No.4

담낭절제술후의 내시경적역행성담췌관조영술의 진단소견과 치료

김원석(Won Seok Kim),조형철(Hyung Chul Cho),조장현(Jang Hyun Cho),추진호(Jin Ho Chu),김명원(Myung Won KIm),오기창(Ki Chung Oh),강명원(Myung Won Kang),임연근(Yun Kun Lim),여향순(Hyang Soon Yeo),박홍배(Hong Bae Park) 대한내과학회 1998 대한내과학회지 Vol.54 No.5

N/A Objectives: Accurate assessment of the retained or recurrent bile duct stone is important in postcholecys-tectomy patients who have signs of cholangitis or other pancreatobiliary disease. This study was performed to evaluate the diagnostic and therapeutic reliability of endoscopic retrograde cholangiography in posteholecystectomy patients. Methods: From October 1975 through August 1995, we reviewed 311 patients retrospectively who had undergone on cholecystectomy, Our study was based on assessment of their age, sex, symptoms and physical findings, comparison between sonographic and endoscopic retrograde cholangiographic findings, and stone removal rate using endoscopic sphincterotomy, basket, electrohydraulic lithotripsy. Results: The ratio of male to female was 1:1.67, and the mean age was 56±14.3 years. After cholecystectomy, they visited our clinic within 2 years in 25 patients (8.4%), beyond 2 years in 286 patients (91.6%). Most common symptoms and physical findings were epigastric pain or tenderrs (63.6%). We detected bile duct stone using endoscopic retrograde cholangiography 219 patients. In case of the bile duct stone, we successfully removed it using endoscopic sphincterotomy, basket, electrohydraulic lithotripsy in 194 patients (88.6%). Conclusion: From the above results, we concluded that endoscopic retrograde cholangiography was the most effective diagnostic and therapeutic method in postch-olecystetomy patients who have signs of cholangitis.

키토올리고당과 토종꿀 혼합조성물의 항균 및 면역 활성

김원석 ( Won Seok Kim ),최창용 ( Chang Yong Choi ),장미경 ( Mi Kyeong Jang ),나재운 ( Jae Woon Nah ) 한국키틴키토산학회 2011 한국키틴키토산학회지 Vol.16 No.2

Chitosan is an attractive biomaterial for wound care because of its biocompatibility and intrinsic hemostatic and antimicrobial properties. Honey has been used since ancient times for the treatment of some respiratory diseases and for the healing of skin wounds. In this study, we investigated the effect of mixture of oligochitosan and herbal honey(chito/H) on atopic dermatitis treatment. The effect of chito/H was analyzed by clinical skin features of atopic dermatitis skin lesions, measuring the serum lgE, and investigated skin surface using an optical microscope. The scores of skin symptom level and the serum IgE were decreased after treatment with chito/H compared with durg as a control group. These results suggest that the chito/H may be an alternative substance for the treatment of atopic dermatitis.