cDNA Microarray를 이용한 구강편평세포암종 세포주에서 $Taxol^{(R)}$과 Cyclosporin A로 유도된 유전자 발현양상

김용관,이재훈,김철환,Kim, Yong-Kwan,Lee, Jae-Hoon,Kim, Chul-Hwan 대한악안면성형재건외과학회 2006 Maxillofacial Plastic Reconstructive Surgery Vol.28 No.3

It is well-known that paclitaxel($Taxol^{(R)}$), which is extracted from the pacific and English yew, has been used as a chemotherapeutic agent for ovarian carcinoma and advanced breast carcinoma and Cyclosporin A, which is highly lipophilic cyclic peptide and isolated from a fungus, has been also used as an useful immunosuppressive drug after transplantation and is associated with cellular apoptosis. Since 1953, in which James Watson, Rosalind Franklin and Francis Crick discovered the double helical structure of DNA, a few kinds of techniques for identifying gene expression have been developed. In postgenomic period, many of researchers have used the DNA microarray which is high throughput screening technique to screen large numbers of gene expression simultaneously. In this study, we searched and screened the gene expression in the oral squamous cell carcinoma cell lines treated with $Taxol^{(R)}$, cyclosporin or cyclosporin combined with $Taxol^{(R)}$ using cDNA microarray. The results were as following; 1. It was useful that the appropriate concentration of Cyclosporin A and $Taxol^{(R)}$ used in oral squamous cell carcinoma cell line was under 1${\mu}g/ml$ and 3${\mu}g/ml$. 2. In the experimental group in which $Taxol^{(R)}$ and $Taxol^{(R)}$ + Cyclosporin A were used, the cell growth was extremely decreased. 3. In the group in which Cyclosporin A was used, the MTT assay was rarely decreased which means the activity of succinyl dehydrogenase is remained in mitochondria but in the group in which the mixture of Cyclosporin A and $Taxol^{(R)}$ were used, the MTT assay was extremely decreased. 4. In the each group in which Cyclosporin A(3 ${\mu}g/ml$) and $Taxol^{(R)}$(1 ${\mu}g/ml$) were used, the cell arrest was appeared in $G_2/M$ phase and in the group in which $Taxol^{(R)}$(3 ${\mu}g/ml$) was used, the cell arrest was appeared in both S phase and $G_2/M$ phase. 5. In the oral squamous cell carcinoma cell line treated with $Taxol^{(R)}$, several genes including ANGPTL4, RALBP1 and TXNRD1, associated with apoptosis, SUI1, MAC30, RRAGA and CTGF, related with cell growth, HUS1 and DUSP5, related with cell cycle and proliferation, ATF4 and CEBPG, associated with transcription factor, BTG1 and VEGF, associated with angiogenesis, FDPS, FCER1G, GPA33 and EPHA4 associated with signal transduction and receptor activity and AKR1C2 and UGTA10 related with carcinogenesis were detected in increased levels. The genes that showed increaced expression in the oral squamous cell carcinoma cell line treated with Cyclosporin A were CYR61, SERPINB2, SSR3 and UPA3A which are known as genes associated with cell growth, carcinogenesis, receptor activity and transcription factor. The genes expressed in the HN22 cell line treated with cyclosporin combined with $taxol^{(R)}$ were ALCAM and GTSE1 associated with cancer invasiveness and cell cycle regulation.

P³²를 이용(利用)한 삼종토양(三種土壤)에 대(對)한 인산효율(燐酸?率)의 진단(診斷)

김용관,홍황규,Kim, Yong Kwan,Hong, Hwang Gyu 한국산림과학회 1970 한국산림과학회지 Vol.10 No.1

육묘장토양(育苗場土壤)의 인산효율(燐酸?率)을 진단(診斷)하기 위하여 가상(假想) 묘상토양(苗床土壤)으로서 3개소(個所)를 설정(設定)하고, 채취(採取)된 이들 토양(土壤)이 갖는 인산효율(燐酸?率)을 $P^{32}$ 표식과석(標識過石)을 이용(利用)하여 측정(測定)한 바 1. 토양내(土壤內)에서의 $P^{31}$ 농도(濃度)의 저하율(低下率)은 shaking time 32시간후(時間後)에 Soil A가 92.2%로 가장 심(甚)하였고 Soil-B, Soil C는 비교적(比較的) 안정(安定)된 31.4%를 시현(示顯)하였다. 2. 인산농도(燐酸濃度)의 저하(低下)는 shaking 당초(當初)에는 급격(急激)히 나타났으나 8시간후(時間後)에는 점차완만(漸次緩慢)해 졌다. 3. $P^{32}$의 흡착저하상(吸着低下相)도 $P^{31}$과 거이 흡사(恰似)한 경향(傾向)을 보였다. 4. 공시토양(供試土壤)은 다같이 pH 상승(上昇)에 따라 흡착도(吸着度)가 감소(減少)되였다. 이상(以上) 몇가지 사실(事實)로 보아 묘포설정시(苗圃設定時) 사전(事前)에 토양(土壤)이 갖는 본래(本來)의 인산효율(燐酸?率)의 진단(診斷)을 하여 흡착고정도(吸着固定度)가 낮은 곳을 택(擇)한다면 최소한(最少限) 인산질비료(燐酸質肥料)에 의한 비배관리면(肥培管理面) 만은 어느 정도(程度) 허실(虛實)을 막을 수가 있다고 사료(思料)된다. An observation was made to diagnose phosphorus efficiency to different soil texture through employing $P^{32}$ labeled Calcium Superphosphate, and following facts were observed. 1. The decreasing ratio of $P^{31}$ intensity in the soils was differ according to the shaking time of soil solution with $P^{32}$ labled calcium superphosphate, and it was observed that after 32 hours, shaking the decreasing ratio of $P^{31}$ intensity in Soil-A, Soil-B and Soil-C were 96.2%, 31% and 37% respectively. 2. In begining of shaking, the decrease of phosphorus intensity was rapid becoming gradually slow afterwards. 3. The adsorption and fixation of $P^{32}$ have shown the same tendency as $P^{31}$. 4. Along with the ascending of pH, all tested soils showed the decrease of adsorption ratio in general.

실외 , 실내 ( 아파트 ) 및 지하상가 공기중 진균 포자 분포에 관한 조사 - 1995 년 하절기 ( 6 , 7 , 8 월 )

김용관(Yong Kwan Kim),김규언(Kyu Earn Kim),이현희(Hyun Hee Lee),박경화(Kyoung Hwa Park),이영진(Young Jin Lee),이기영(Ki Young Lee) 대한소아알레르기호흡기학회(구 대한소아알레르기 및 호흡기학회) 1996 소아알레르기 및 호흡기학회지 Vol.6 No.2

목적 : 실외 공기 중 진균 포자의 분포에 관한 보고는 있었으나 실내 및 지하상가 공기중의 포자 분포에 대한 연구는 전무한 상태이고, 실내외에 많이 존재하고 있는 진균의 항원성을 연구하기 위한 첫번째 단계로 우리의 생활 주변에서의 진균 분포를 알아보고자 본 연구를 계획하게 되었다. 방법 : 1995년 6월 1일부터 8월 31일까지 하절기 3개월 동안 실외(영동세브란스병원), 실내(여의도 소재 아파트) 및 지하상가(강남역) 각각의 장소에서 1주일에 3회씩 Buckard Personal Volumetric Air Sampler를 사용하여 실리콘 기름(silicone grease)을 바른 현미경용 슬라이드 위에 시료를 5분 동안(10L of air/min) 채집한 뒤에 현미경하에서 포자의 종류와 수를 확인한 뒤에 이를 공기 입방 미터당 포자수(spores/㎥ of air)로 환산하여 포자의 분포도를 분석하였다. 결과 : 1. 실외에서 채집된 포자수는 6월에 가장 많았으며(1,903 sp/㎥), 그 평균 포자수는 1,843 sp/㎥이었다. 이들 포자를 종류별로 분석해 보면 Cladosprium (927 sp/㎥), Leptospira (190 sp/㎥), Drechslera (132 sp/㎥) 둥의 순으로 분포도가 높았다. 2. 실내(아파트)에서 채집된 포자수는 7월에 가장 많았으며(647 sp/㎥), 그 평균 포자수는 592 sp/㎥이었다. 이들 포자를 종류별로 분석해 보면 Cladosprium (313 sp/㎥), Leptospira (51 sp/㎥), Drechslera (29 sp/㎥) 둥의 순으로 분포도가 높았다. 3. 지하상가에서 채집된 포자수는 8월에 가장 많았으며(1,021 sp/㎥) , 그 평균 포자수는 851 sp/㎥ 이었다. 이들 포자를 종류별로 분석해 보면 Cladosprium (388 sp/㎥). Leptospira (88 sp/㎥), Drechslera (70 sp/㎥) 둥의 순으로 분포도가 높았다. 4. 실외에 분포하고 있던 포자의 수는 실내 및 지하상가의 포자수보다 약 2∼3배 정도 높았고 실외와 실내 포자수 사이에 유의한 관계가 있었으나, 실외와 지하상가 포자수의 사이에는 상관관계가 없었다. 그러나 실외, 실내 및 지하상가의 포자수는 상대습도가 높을수록 증가하는 경향을 보였다. 결론 : 하절기 동안 채집된 포자의 수는 실외에서 가장 많았으나, 포자의 종류는 장소에 관계없이 Cladosporium, Leptospira 및 Dtechslera의 순으로 분포도가 높았다. 따라서 생활 주변에 많이 분포하고 이들 포자의 항원성에 대한 연구와 더불어 진균 포자 분포에 대한 전국적인 역학조사가 필요하다고 사료된다. The number of fungus spores in the air is greater compared to pollen and also is less influenced by seasonal variation. As the first step in the study of the allergenicity of fungus, we surveyed the distribution of fungus spores in the air of outdoors, indoors and underground markets during summer(June, July and August, 1995). We collected spores on the slide glass coated with grease by using the Burkard Personal Volumetric Air Sampler. The frequency of collection was three times per week for five minutes(10L of air/min). Then we counted the number of fungus spores under the light microscope and identified them according to the atlas for spores. The results were as follows; 1. The mean number of spores during June, July and August were 1903sp/㎥, 1731sp/㎥, and 1894sp/㎥ in outdoors, 533sp/㎥, 647sp/㎥, and 595p/㎥ in indoors, and 646sp/㎥, 886sp/㎥, 1021sp/㎥ in underground markets, respectively. 2. The most common species of spores in outdoors were the Cladosporium(927sp/㎥), Leptospira(190sp/㎥), and Drechslera (132sp/㎥) . These were also the most common spores in indoors and underground markets. 3. Although the number of spores in indoors was closely related with that in outdoors, there was no relationship between underground markets and outdoors in the number of spores. 4. The number of spores in outdoors, indoors and underground markets were related to the relative humidity. In conclusion. Cladosporium, Leptospira, and Drechslera were the most common spores in outdoors, indoors and underground markets during summer. Therefore it would be necessary to evaluate the allergenicity of these spores.

컬러영상을 이용한 실시간 COF 필름 복합 검사시스템 개발

김용관(Yong-Kwan Kim),이인환(In Hwan Lee) 한국기계가공학회 2021 한국기계가공학회지 Vol.20 No.10

In this study, an inspection method using a color image is proposed to conduct a real-time inspection of covalent organic framework (COF) films to detect defects, if any. The COF film consists of an upper pattern SR and a lower PI. The proposed system detects the defects of more than 20 μm on the SR surface owing to the characteristics of the pattern, whereas on the PI surface, it detects defects of more than 4 μm by utilizing a micro-optical system. In the existing system, it is difficult for the operator to conduct a full inspection through a high-performance microscope. The proposed inspection algorithm performs the inspection by separating each color component using the color contrast of the pattern on the SR side, and on the PI surface it inspects the bonding state of the mounted chip. As a result, it is possible to confirm the exact location of the defects through the SR and PI surface inspections in the implemented inspection.

해양오염 방제지원시스템에 의한 침몰선 오염사고 대응

김용관(Yong-Kwan Kim),박장번(Jang-Bun Park),도재만(Jae-Man Tho) 한국해양환경·에너지학회 2007 한국해양환경·에너지학회 학술대회논문집 Vol.2007 No.-

선박의 해난에 의한 해양오염사고 발생시, 방제지원시스템을 운용하여 신속하고 체계적인 방제전략 및 전술을 수립함으로써, 사고로 인한 해양환경 피해를 최소화하고 있다. 본 연구에서는 2006년 7월 전북 군산시 새만금방조제 인근 해상에서 침몰한 700톤급 준설선 GA-P1호에 대한 선체인양 및 해양오염사고 처리과정에서 방제지원시스템을 이용한 대응사례이다.

관통상으로 응급실을 내원한 내국인과 외국인 환자의 진료의 차이

김용관 ( Yong Kwan Kim ),장용수 ( Yong Soo Jang ),강구현 ( Gu Hyun Kang ),최정태 ( Jung Tae Choi ),전후 ( Hoo Jeon ),김진호 ( Jin Ho Kim ) 대한외상학회 2010 大韓外傷學會誌 Vol.23 No.2

Purpose: With the increasing numbers of foreign residents in Korea, the need for an emergency medical care system for foreign patients seems to be growing. Sometimes, a foreigner admitted to an emergency room is not treated sufficiently due to the absence of insurance, facility in the Korean language, and a guardian. The management of a foreigner with trauma in the ER is difficult due to various problems such as social and economic status. The purpose of this study was to investigate the current management status of foreigners with penetrating wounds in the emergency room. Methods: This study is an analysis of 580 patients that were diagnosed with penetrating wounds in one teaching Hospital from Jan. 1, 2008 to Dec. 31, 2008. We analyzed results according to nationality, alcohol ingestion, intentional or accidental trauma, trauma mechanism, injury severity, management time in the ER, and outcome in the ER. Results: Of the total 580 patients, 486 patients (83.8%) were native Koreans and 94 patients (16.2%) were foreigners. According to the Revised Trauma Score, the average score of native Korean patients was 7.808, and the average score of foreign patients was 7.638. Of native Korean patients, 22.6% had knife wounds while 38.3% of foreign patients did. Of native Korean patients, 17.3% experienced intentional trauma while 33.0% of the foreign patients did. Of native Korean patients, 22.5% had ingested alcohol while 49.4% of the foreigners had. Of native Korean patients, 10.5% were admitted while 7.6% of the foreign patients were. Of native Korean patients, 14.2% were discharged against medical advice (DAMA), while 18.5% of foreign patients were. Of native Korean patients, 1.2% ran away while 8.7% of the foreign patients did. Conclusion: Stabbing was the most common cause of penetrating wounds in foreigner patients in this study. Intentional trauma was more common in foreigners with penetrating wounds than in native Koreans. The severity was higher in foreigners with penetrating wounds than it was in native Koreans, and patients who ran away or were discharged against medical advice were more commonly foreigners with penetrating wounds. Social insurance or policy is needed for the management of foreigners with penetrating wounds. (J Korean Soc Traumatol 2010;23:102-106)

CG산업에서 Simulation 표현의 기술적 활용에 대한 연구

김용관(Kim Yong-Kwan) 한국만화애니메이션학회 2009 만화애니메이션연구 Vol.- No.17

본 연구는 3차원 컴퓨터 그래픽 제작 산업분야에서 최근 가장 경쟁력 있는 분야로 대두되고 있는 연구/개발(R&D) 분야에서 “불의 표현을 중심으로 국내 관련업체들의 기술적 한계를 제시하고 해외 및 국내 애니메이션 제작에서 성공한 기술적 R&D의 과정과 그 결과를 통해 이러한 기술적 한계를 어떻게 극복해야 하는지에 대한 해답을 제시함으로써 과도기를 거쳐 제2의 도약기를 준비하는 국내 컴퓨터 그래픽 애니메이션 산업의 부흥에 도움이 되고자하는 목적으로 연구를 진행하고 있다. 흔히 CT와 IT의 상호 보완적인 특징을 활용한다면 이러한 문제 해결을 넘어 기술적 우위를 확보할 수 있다. 우리는 특히 컴퓨터 그래픽 제작 과정 중 물. 불, 바람, 구름 등의 Simulation System분야와 수십만 캐릭터의 집단행동 또는 단체행동 System 활용도를 중심으로 연구를 진행하였다. 그 중에서 본 논문은 불의 표현에 중점을 두고 실제 작업에 활용된 데이터를 기준으로 그 해답을 제시하였으며 기존의 국내에서 사용하던 전통적 활용방법과 각 연구단체에서 활발하게 진행 중인 프로젝트를 소개하고 본 연구의 적용과정과 결과를 소개함으로써 현업 종사자들에게 향 후 R&D분야의 나아갈 방향을 제시하고자한다. 창조적인 부재에 너무 집착한 나머지 기술적인 연구 개발부분에의 소홀함이 향 후 미국이나 일본과의 더 큰 기술적 문제로 대두될 수 있음을 명심하고 지금부터라도 과감하고 결단력 있는 기술력의 연구 개발이 필요한 시점이다. This study purpose development cg technique our nation because present Technique limit of Computer graphic R&D that the best competition of 3D Computer graphic part and present a answer of how to overcome Technique limit of Computer graphic R&D with success example of Computer graphic R&D an advanced country. if we make full of mutuality supplementation of C.T and I.T then hold a dominant position and the over the limit solution We researches on multi or group simulation of cyber character and water, fire, wind, fog Simulation System of Computer graphic and purpose development of direction Computer graphic R&D to people in Computer graphic the result of this study and introduction of project of a research institution and inflection method this time is a point of R&D time of determined and resolution instead creative part

국내 야생박쥐 코로나바이러스 감시현황 및 결과

김용관 ( Yong Kwan Kim ) 대한인수공통전염병학회 2019 창립총회 및 학술대회 초록집 Vol.2019 No.1

Pterygopalatin fossa 로 전위된 상악 매복지치 발치 치험례

김용관(Yong Kwan Kim),장명진(Myung Jin Jang),장현석(Hyun Suk Jang) 대한악안면성형재건외과학회 1994 Maxillofacial Plastic Reconstructive Surgery Vol.16 No.2

The surgical removal of the wisdom teeth is obligate when forceps extraction fails or when the wisdom teeth are impacted. The surgical removal of impacted maxillary third molars is a commonly performed procedure usually associated with few complications & little morbidity. The most frequent complications are tooth root fracture, maxillary tuberosity fracture, tooth displacement into the maxillary sinus & oroantral fistula formation. A rarely reported complication is the displacement of a tooth into the infratemporal fossa. The method of prevention of this complication is by the placement of either a finger or periosteal elevator posterior to the tooth during extraction. To remove the disploaced upper third molar is very difficult & has many complications, e.g., persistent bleeding k nerve damage. When the wisdom teeth is displaced, it is initially necessary to gain access to bone by developing a mucoperiosteal flap. Adequate access to the tooth is then achieved by conservative removal of bone. Finally, an unimpeded path of delivery is developed by additional bone removal or, preferably planned sectioning of the tooth. There are many approaching techniques to remove the displaced upper third molar. This following report describes the surgical technique of displaced upper third molar in the pterygopalatine fossa by the midpalatal & transpharyngeal approach.

EXFOLIATIVE CYTOLOGISCHE UNTERSUCHUNGEN ÜBER DIE BEEINFLUSSUNG AM MUNDSCHLEIMHAUT DURCH METALL-UND KUNSTSTOFF(ZAHNÄRZTLICHE PROTHESE)

Yong Kwan Kim(金用琯) 대한치과의사협회 1970 대한치과의사협회지 Vol.8 No.3