계단식 단부 형태의 탄소섬유시트로 보강된 RC보의 구조거동 평가에 관한 실험적 연구

김영희,김희선,Kim, Young-Hee,Kim, Hee-Sun 한국복합신소재구조학회 2012 복합신소재구조학회논문집 Vol.3 No.4

This paper presents experimental studies aiming at evaluation of structural behaviors of RC (Reinforced Concrete) beams externally strengthened with taper ended CFRPs(Carbon Fiber Reinforced Polymers). Experiments are performed with RC beams having different numbers of CFRP layers and length of each layer. The beams are subjected to four point-bending with simply supported condition. Test results of taper ended CFRPs and non-tapered CFRPs are compared and the better strengthening effect is observed from tapered ended CFRPs.

휨 보강용 섬유시트연구동향소개

김영희,김희선,Kim, Young-Hee,Kim, Hee-Sun 한국복합신소재구조학회 2013 복합신소재학회논문집 Vol.4 No.1

요가 프로그램이 재가노인의 우울, 삶의 질, 일상활동장애에 미치는 효과

김영희,박금숙,양경희,정헌영,양희정,Kim, Young-Hee,Park, Kum-Sook,Yang, Kyung-Hee,Jeong, Heon-Young,Yang, Hee-Jung 대한예방한의학회 2013 대한예방한의학회지 Vol.17 No.3

Objective : The purpose of this study was to investigate the effects of yoga program on depression, quality of life, and difficulty of daily activities in community elders. Methods : A Nonequivalent control group pretest-posttest design was used for this study. Elderly people who agreed to participated in the study were assigned to a experimental group(n=17) or a control group(n=17). The yoga program conducted 90minutes, 2times a week for 1 year. Study outcomes were measured by structured questionnaires from March, 2012 to Feb., 2013. For data analysis, Chi-square test, t-test, and Mann-Whitney U test were performed using SPSS version 17.0. Results : Depression was not significant difference between the two groups in pretest and posttest. But there was significant differences between the two groups in quality of life (t=3.984, p<.001), and difficulty of daily activities (t=-3.083, p=.004). Therefore, the experimental group showed significant improvement in quality of life and reduce difficulty of daily activities. Conclusion : The yoga program was effective in increasing quality of life and decreasing difficulty of daily activities in community elders. When developing exercise program to improve health in older adults, it is important to consider physical exercise plus general health management.

섬유 재질에 대한 천연 살충·살균제의 적용성 평가

김영희,홍진영,정미화,조창욱,김수지,이정민,최정은,Kim, Young-Hee,Hong, Jin-Young,Jung, Mi-Hwa,Jo, Chang-Wook,Kim, Soo-Ji,Lee, Jeung-Min,Choi, Jung-Eun 국립문화재연구소 2011 保存科學硏究 Vol.32 No.-

Wood vinegar and Asarum sieboldii Miquel were selected as candidate materials showed antimicrobial activity and insect repellent activity. These natural pesticides have its own color and these could cause color difference on fabric materials. In the present study, we investigated the color difference of undyed and dyed fabrics to evaluate negative effect of wood vinegar and A. sieboldii Miquel. Undyed and dyed fabrics were exposed to natural pesticides of various concentrations for six months in relative humidity 70% and temperature $28^{\circ}C$. After exposure of pesticides, color difference (${\Delta}E*$) were investigated at two weeks intervals for six months. As a results, dyed cotton, silk and undyed silk fabrics exposed wood vinegar were not nearly changed in their colors, but color of only undyed cotton fabric was clearly changed by wood vinegar. Especially color difference by wood vinegar on undyed cotton fabric was most distinct as the concentration increased. On the other hand, all of fabrics exposed A. sieboldii Miquel were not nearly changed in their colors for six months. Therefore, this study first suggests that wood vinegar and A. sieboldii Miquel as natural insecticides could be used to conserve for textile cultural properties from insects and microorganism, but wood vinegar couldn't use the high concentration on undyed cotton fabric. 천연물로부터 항균 및 방충작용에 효과적인 물질을 검색한 결과, 목초액과 세신추출물이 강한 생물활성을 나타내는 후보물질로 선발되었다. 천연 항균 및 살충제로서 선발된 목초액과 세신추출물은 자체에 색을 지니고 있어 이들을 이용하여 문화재 보존 처리제로 사용하였을 경우 유기질 문화재 재질에 나쁜 영향을 줄 수 있다. 따라서 본 연구에서는 섬유 재질에 대한 영향을 평가하기 위하여 무염색 섬유 시편과 염색 섬유 시편을 대상으로 두 종의 천연 살충소재를 $28^{\circ}C$ 온도와 70% 습도 조건에서 노출 실험을 실시하였으며, 천연 살충소재 처리 후 6개월 동안 이들 섬유의 색변화를 2주 간격으로 측정하였다. 그 결과 세신추출물의 경우 무염색 섬유뿐만 아니라 천연 염색한 붉은색과 푸른색의 섬유에서 또한 색변화가 크게 나타나지 않았으며, FT-IR spectrum 분석 결과에서도 초기 시편과 비교 시 큰 변화가 관찰되지 않았다. 그러나 목초액의 경우 무염색 면섬유에 있어서 3주차부터 뚜렷한 색변화가 관찰되었으며, FT-IR spectrum 분석 결과에서도 초기 시편과 비교했을 때 특정 파장대의 피크가 없어지는 것으로 나타났다. 그러므로 목초액의 경우 유기질 문화재 보존처리제로 적용할 경우 섬유 재질에 영향을 미칠 수 있어 사용에 제한을 두어야 한다.

Effect of Growth Conditions in the Attachment of Salmonella typhimurium to the Host Cells

김영희,김상웅,강호영,Kim, Young-Hee,Kim, Sam-Woong,Kang, Ho-Young Korean Society of Life Science 2005 생명과학회지 Vol.15 No.4

Salmonella typhimurium TML이 Int-407 숙주세포에 부착하는 정도는 S. typhimurium이 낮은 농도의 철이 함유된 배지에서 배양되었을 때보다 LB 액체배지 또는 높은 농도의 철이 함유된 배지에서 생육한 것에서 약 10배 정도의 높은 수준으로 관찰되었다. 고농도의 철이 포함된 배양조건이 살모넬라가 숙주세포에 부착시키는 정도를 향상시키는데 반해, 칼슘, 코발트, 구리, 인산, 마그네슘 그리고 망간과 같은 다른 양이온은 그렇지 않다. 이것은 아마도 철이 Salmonella가 부착에 필요한 요소들의 발현을 활성화하는 역할을 하는 것으로 추정된다. 철 농도에 따른 부착정도의 차이들은 type 1 fimbriae, mannose resistant hemagglutinin과 flagellum등을 생성하지 않는 다양한 S. typhimurium 돌연변이주들에서도 관찰되는 것으로 미루어보아 이들 구조체들과 상관이 없는 밝혀지지 않은 어떤 인자가 부착성 증가에 관여하는 것으로 사료된다. fur유전자가 불활성화된 S. typhimurium 돌연변이주의 부착성이 야생형 Salmonella와 유사한 방식으로 철에 의해 조절되었는데, 이는 철 농도에 따른 부착성의 변화에 관여하는 잠재적인자의 발현이 Fur 단백질과 독립적으로 이루어진다는 것을 나타낸다. An approximately 10-fold higher level of adherence of Salmonella typhimurium strain TML to Int-407 cells was observed with organisms grown in Luria broth or in high-iron containing medium than those grown in low-iron containing medium. Iron specifically enhanced adherence, while other cations such as calcium, cobalt, copper, potassium, magnesium and manganese did not. It was suggested that iron did not act as a passive ligand - probably it stimulated production of bacterial factors necessary for adherence. A similar pattern of iron modulation of adhesiveness was also seen in Salmonella mutants with single or different combinations of multiple mutations in genes encoding the mannose sensitive hemagglutinin (type 1 fimbriae), mannose resistant hemagglutinin and flagellum. The adhesiveness of an isogenic fur mutant was modulated by iron in a manner similar to the wild-type strain, suggesting that iron modulation of adherence is independent of the fur gene product.

고점도 지류 및 섬유 문화재의 점도 측정 개선 방법 연구

김영희,홍진영,조창욱,김수지,이정민,서민석,최경화,Kim, Young-Hee,Hong, Jin-Young,Jo, Chang-Wook,Kim, Soo Ji,Lee, Jeung-Min,Seo, Min Seok,Choi, Kyoung Hwa 국립문화재연구소 2013 保存科學硏究 Vol.34 No.-

지류, 섬유, 목재와 같은 유기질 문화재는 주요 화학성분으로서 셀룰로오스로 구성되어 있다. 셀룰로오스는 글루코오스가 분자내 또는 분자간 수소결합으로 고분자 결정성 구조를 이루고 있다. 특히 셀룰로오스의 중합도는 종이나 섬유의 강도와 밀접한 관련이 있으며, 종이나 섬유에 있어서 열화의 진행정도나 보존성을 나타내는 지표로써 사용된다. 일반적으로 이러한 셀룰로오스의 분자량을 측정하는 방법으로 TAPPI 표준방법인 CED (cupriethylene diamine)용액을 이용한 점도측정법을 많이 사용하고 있다. 목재 섬유로 제조된 종이의 경우 TAPPI 표준 방법 T230법에 의거하여 셀룰로오스 점도를 측정한다. 그러나 우리나라 종이나 섬유의 주요 원료인 닥나무 인피섬유, 저마, 면 등은 목재 셀룰로오스 섬유에 비해 분자량이 크고 중합도가 높아 현재까지 주로 사용되던 T230법으로는 정확한 점도 측정이 어렵다. 따라서 본 연구에서는 고점도 측정방법인 TAPPI 표준 방법 T254법에 의거하여 저농도 CED 용액으로 섬유 깊숙이 용액을 침투시켜 해리시킨 뒤 고농도의 CED 용액으로 완전히 용해시키는 방법을 이용하여 한지의 점도를 측정하였고, 열화시편에 적용하여 종이와 섬유의 열화에 의한 손상정도를 측정하였다. Paper, textile and wood materials are mainly consisted of cellulose. Cellulose is high molecule and make up the strong crystalline structure by hydrogen bonds. In particular, the polymerization degree of cellulose are closely related to the strength of fiber, and the permanence. the useful life of fiber, also depends on the degradation of this substance. The viscosity of cellulose is considered to be an important indicator of fiber damage in high molecule polymers. The viscosity measurements with CED solution is used to measure the molecular weight and the degree of polymerization of cellulose. Cellulose viscosity of wood fibers is measured with TAPPI standard method T230. However, TAPPI standard method T230 is difficult to completely dissolving the cellulose of high molecular weight and large degree of polymerization, such as Korea traditional papers and fabrics made with mulberry, ramie, cotton fibers. In this study, The high viscosity of hanji and fabric was measured with TAPPI standard method T254. T254 method is that the cellulose specimen with the proper amount of weaker (0.167M CED) solution, and completely dissolved with the stronger (1.0M CED) solution. It was found that cellulose with high degree of polymerization was dissolved more easily in general CED method.

Characterization of Salmonella Adhesins Required for Colonization of Animals

김영희,김상웅,강호영,Kim Young Hee,Kim Sam Woong,Kang Ho Young Korean Society of Life Science 2005 생명과학회지 Vol.15 No.2

살모넬라는 질병을 일으키기 위해서 장내의 상피세포에 먼저 부착하여 집락을 이루게 된다. 이에 따라 살모넬라는 부착에 관여하는 세포내 몇 몇 소기관들을 합성한다. 이러한 소기관에는 type 1 fimbriae, plasmid-encoded fimbriae, long polar fimbriae 그리고 thin aggregative fimbriae 등이 있다. 본 논문에서는 type 1 fimbriae, thin aggregative fimbriae, LP fimbriae, 그리고 PE fimbriae 각각을 결손시킨 변이주와 4종류를 모두 결손시킨 변이주를 만들 수 있었다. 변이주들에 대해서 세포배양 부착성 실험을 한 결과, 각각을 결손시킨 변이주들은 몇몇 mammalian 세포주에서 야생형 살모넬라와 동일한 양상으로 부착성을 나타내었고 마우스 실험에서 야생형과 거의 마찬가지로 독성을 가지고 있었다. 반면, 4종류의 fimbriae가 모두 결손된 균주는 닭에서는 독성을 나타내었으나 마우스에서는 매우 약독화되어있음을 확인할 수 있었다. 이상의 결과로부터 오는 차이점은 닭과 비교하여 마우스에서 살모넬라의 부착을 매개하는 표면 구조에 연관성이 있음을 제시하였다. Following ingestion, Salmonella must adhere to and colonize the intestinal epithelium of the host in order to establish infection. S. typhimurium synthesize several appendages that are believed to mediate attachment. These include type 1 fimbriae, plasmid-encoded (PE) fimbriae, long polar (LP) fimbriae, and thin aggregative fimbriae. However, the precise roles of these putative adhesins remain unclear, due to conflicting data in the literature. We constructed strains lacking four different fimbriae including type 1 fimbriae, PE fimbriae, LP fimbriae, and thin aggregative fimbriae, as well as strains lacking each fimbriae alone. In cell culture adhesion assays, these mutants adhered to several mammalian cell lines as well as wild-type S. typhimurium. These strains were also screened for virulence in mice, and all strains were virulent or nearly as virulent as their wild-type parents. In contrast, When a strain lacking four fimbriae was screened for virulence in chicks, it was found to be highly attenuated. This suggests a role for either type 1 fimbriae, PE fimbriae, LP fimbriae or thin aggregative fimbriae or a combination of thease fimbriae in the colonization of chicks. It also suggests that differences exist with respect to the surface structures that mediate attachment of Salmonella in chicks as compared with mice.

Lactobacillus plantarumCLP-1이 돼지바이러스에 미치는 효과

이건희(Gun-Hee Lee),김영희(Young-Hee Kim),조현아(Hyeon-A Cho),강성기(Sung-Gi Kang),김동건(Dong-Gun Kim) 한국생물공학회 2011 KSBB Journal Vol.26 No.1

To isolate Lactic acid bacteria for animals, we have screened from Kim-chi, swine intestine, swine feces, and dairy products by random selection and anti-viral, antipathogenic bacteria test. Among them, CLP-1 shown that inhibitory effect against rotavirus, porcine epidemic diarrhea (PED) virus, Salmonella sp, and E.coli. By examining biological property, API-ZYM and identified Lactobacillus plantarum by 16S rDNAgene sequence. CLP-1 determined resistance to low pH and bile salt. Futhermore, the cell body of CLP-1 adhered to the intestinal epithelium tissue of swine and Caco-2 cell. CLP-1 was examined on cell immune system modulating activity in vitro. The whole cell and cell culture supernatant was increasing of interferon-β activity. And then, CLP-1 increased prevention effect by Salmonella enteritidis infection in SPF chickens. And we determined similar result in pigs.

대학생의 장애 이해에 대한 태도 연구

김영희,Kim, Young-Hee 대한물리치료과학회 2002 대한물리치료과학회지 Vol.9 No.1

The present study focuses on the relation of the attitudes of university students to disabled by using modified disability factor scales(DFS, Siller). As for the attitude areas, it relies on the eight categories DFS provides. The 8 categories that specify each attitude area are: generalized rejection, distressed identification, inferred emotional consequences, imputed functional limitation, authoritarian virtuousness, rejection of intimacy, interaction strain, mainstreaming. This study is based on a series of questionnaire researches conducted on 322 students, over the period from October 19th to November 14th, 2001. The questionnaire carries 50 items, covering the 8 attitude areas and each item has 5 choices. The subjects' responses to each of the areas are examined in their relations to the four conditions, as they are analysed by t-test and one way ANOVA. The conclusion drawn from the research are as follows: 1. There is no significant difference between the students of the department concerned with the problem of disability, and those of the department unconcerned. But the responses are significantly different in the three areas of generalized rejection, rejection of intimacy and interaction strain(1st condition, P<.05). 2. The responses are significantly different in 6 areas except for the two, distressed identification and inferred emotional consequences, between the students who have volunteered services as helpers of the disabled and those who have no such experiences(2nd condition, P<.05). 3. the responses are significantly different between those who have volunteered long-time services and those who are far less experienced(3nd condition, P<.05). 4. There is no significant difference, whether the subjects have disabled persons around them or not. But generalized rejection, authoritarian virtuousness, rejection of intimacy, interaction strain & mainstreming show significant differences(4th condition, P<.05). 5. Generalized rejection, rejection intimacy and interaction strain show significant differences in every pair of the 4 condition. 6. Distressed identification and inferred emotional consequence show no significant difference between the two conditions in every pair of the 4 considerations.

Enterobacter cloaceae K41 plasmid의 중금속 저항성

김영희,이상준,정영기,정경태,Kim Young-Hee,Lee Sang-Jun,Jeong Yong-Kee,Chung Kyung-Tae 한국생명과학회 2005 생명과학회지 Vol.15 No.4

담수 식물 수초의 근계에 부착하는 미생물 중 중금속에 높은 저항성을 가지는 균을 분리하고 이들 중 Enterobacter cloaceae K41를 대상으로 생육최적조건을 검토한 결과는 LB배지에 $1\%$ yeast extract, $1\%$ lactose, $1\%$ NaCl, pH7.0, 최적 온도는 $37^{\circ}C$, 24시간 진탕배양 이었으며 중금속 첨가 시에는 침전을 막기 위하여 Nutrient 배지로 대체하였다. 이 분리균주와 표준균주인 Enterobacter cloaceae KCTC2519를 대상으로 중금속인 구리와 카드뮴이온에 대한 최소생육저지농도(MIC)를 비교한 결과 분리균주인 E. cloacear K41은 구리는 150 ppm, 카드뮴은 50 ppm농도까지 생육이 확인되었으나 표준균주는 구리 50ppm에서 생육이 확인되었으나 카르뮴이나 두 혼합 중금속에서는 확인되지 않는 차이를 나타내었다. 두 균주를 대상으로 유전적 성상을 비교한 결과 분리균주에선 plasmid가 검출되었으나 표준균주에는 없었다. 그리고 분리균주에서 6.4Kb 절편의 plasmid를 분리하여 구리, 카드뮴의 중금속에 민감한 균주인 E, coli $DH5{\alpha}$에 형질전환 시켜 생육에 영향을 미치는 정도를 비교하였다. 그 결과 형질전환 균주의 두 중금속에 대 한 최소생육저지농도가 구리는 7배, 카드뮴은 6배로 증가한 것을 알 수 있었다. 또한 중금속 흡착률은 형질전환 균주가 E. ccli $DH5{\alpha}$보다 구리가 1.3배, 카드뮴은 1.5배 증가함을 알 수 있었다. 따라서 이 plasmid가 중금속 저항성을 증가시키는데 관여하는 것으로 보였다. A natural habitat bacterium, Enterobacter cloaceae K41 was isolated from fresh water plant root and identified. This strain was used to investigate heavy metal resistance. The optimal growth conditions of the bacterium were LB medium containing$1\%$ yeast extract, $1\%$ lactose, $1\%$ NaCl, pH 7.0, at $37^{\circ}C$, and for 24 hours on a shaker. The minimal inhibitory concentration (MIC) of heavy metals against E. cloaceae KCTC2519 and E. cloaceae K41 was compared. The MIC of E. cloaceae K41 was 150 ppm in Cu, 50 ppm in Cd whereas that of the standard strain was 50 ppm in Cu but no growth was observed either Cd or two mixed heavy metal solution. The presence of plasmid was cleared from the isolated strain whereas no possession from the standard strain. The plasmid from E. cloaceae K41 was transformed into E. coli $DH5{\alpha}$. The MIC of transformed strain increased resistance 7 times in Cu and 6 times in Cd by insertion of this plasmid. The metal adsorption of the transformant was increased 1.3 times in Cu and 1.5 times in Cd indicating the plasmid was responsible for heavy metal resistance.