Watersheds 기반 계층적 이진화를 이용한 단백질 반점 분할 알고리즘

김영호,김정자,김대현,원용관,Kim Youngho,Kim JungJa,Kim Daehyun,Won Yonggwan 한국정보처리학회 2005 정보처리학회논문지B Vol.12 No.3

생물학자가 단백질을 검색하고 분석하기 위해서는 2차원 젤 전기영동(2DGE : Two Dimensional Gel Electrophoresis) 실험을 해야 한다. 실험 결과는 2차원 영상이 생성된다. 2차원 영상에서 단백질 반점의 패턴 분석을 위해 2차원 젤 영상에 펼쳐진 단백질 반점들을 영상처리를 통해 분할하고, 대조 그룹의 단백질 패턴과 비교분석을 통해 밝히고자하는 단백질 반점을 찾아내야 한다. 단백질 반점을 분할하는 알고리즘에 있어서 기존에는 가우시안 함수를 적용하였지만, 최근 들어 형태학 분리개념에 의한 Watersheds 영역기반 분할(Watersheds region-based segmentation) 알고리즘을 활용하고 있다. 그러나 Watersheds 영역기반 분할 알고리즘은 크기가 큰 영상에서 원하는 영역을 신속하게 분할한다는 장점이 있지만, 영상 화소의 그레이 값이 연속적인 경우 실제 반점의 개수 에 비해 과다분할(over-segmentation)되거나 과소분할(under-segmentation)의 문제점을 안고 있다. 이는 마커(marker) 포인트의 설정에 의해 어느 정도 해결할 수 있지만 병합(merge)과 분할(split) 과정을 반복해야 한다. 본 논문은 Watersheds 기반 계층적 이진화 기법을 적용하여 마커 드리븐 Watersheds 영상분할의 문제점을 해결하고자 한다. Biologist must have to do 2DGE biological experiment for Protein Search and Analysis. This experiment coming into being 2 dimensional image. 2DGE (2D Gel Electrophoresis : two dimensional gel electrophoresis) image is the most widely used method for isolating of the objective protein by comparative analysis of the protein spot pattern in the gel plane. The process of protein spot analysis, firstly segment protein spots that are spread in 2D gel plane by image processing and can find important protein spots through comparative analysis with protein pattern of contrast group. In the algorithm which detect protein spots, previous 2DGE image analysis is applies gaussian fitting, however recently Watersheds region based segmentation algorithm, which is based on morphological segmentation is applied. Watersheds has the benefit that segment rapidly needed field in big sized image, however has under-segmentation and over-segmentation of spot area when gray level is continuous. The drawback was somewhat solved by marker point institution, but needs the split and merge process. This paper introduces a novel marker search of protein spots by watersheds-based hierarchical threshold, which can resolve the problem of marker-driven watersheds.

팽이버섯 분열자의 생성원인 및 특성

김영호,공원식,김경수,유창현,김한경,성재모,류영진,김광호 ( Young Ho Kim,Won Sik Kong,Kyung Soo Kim,Chang Hyun You,Han Kyoung Kim,Jae Mo Sung,Young Jin Ryu,Kwang Ho Kim ) 한국균학회 1998 韓國菌學會誌 Vol.26 No.2

기관지내 발생한 과오종치험 1례

김영호,김수성,김공수,Kim, Yeong-Ho,Kim, Su-Seong,Kim, Gong-Su 대한흉부심장혈관외과학회 1983 Journal of Chest Surgery (J Chest Surg) Vol.16 No.1

The origin of hamartoma from the Greek words for "error" and "tumor" is credited to Albrecht who in 1904 described a disorganized arrangement of tissues normally present in an organ and applied also to tumors found in many organs other than the lung. The hamartoma is the most common benign tumor of the lung and revealed accidentally as asymptomatic coin lesion on routine chest X-ray, but the incidence is very low and especially endobronchial origin extremely low. We have been successful surgical experienced one case of a 36-year-old female having endobronchial hamartoma, 4x2.Sx2 cm in size and located at right main bronchial lumen near the carina, which consists of a hard, nodular surfaced mass and adhered to the cartilaginous portion of the right upper lobe bronchus by dense fibrous band and migrate to trachea on expiration or coughing. This case was not suspected by chest X-ray or bronchogram and confirmed bronchoscopy with biopsy. Right pneumonectomy was inevitable because of bronchiectatic change of right bronchus due to tumor obstruction. She was discharged with relatively good general condition on 21 days postoperatively.

수용성카이토산에 의한 마우스태반을 통한 태아로의 방사성스트론튬 급성 전이 억제

김영호,범희승,김지열,Kim, Young-Ho,Bom, Hee-Seung,Kim, Ji-Yeul 대한방사선방어학회 1997 방사선방어학회지 Vol.22 No.4

카이토산은 천연무독성 착화제의 일종으로 유전적 독성이 거의 없으며, 방사성스트론튬의 마우스 체내 축적을 감소시킨다고 알려져 왔다. 본 연구에서는 임신 17일째 마우스에서 방사성스트론튬 오염전 후에 수용성카이토산을 공급함으로써 오염 후 태반을 통한 태아로의 방사성스트론튬 급성 전이를 얼마나 효과적으로 억제할 수 있는 가를 알아보고자 하였다. 수용성카이토산 분말을 50일간 일반식이와 혼합하여 10% (Group 1), 1%(Group 2)를 공급하고 임신 17일째에 방사성스트론튬을 오염시킨 군의 경우 일반식이를 공급한 대조군과 방사성스트론튬 오염 후 카이토산 10%, 1%분말을 공급한 군(Group 3, Group 4)에 비해 태반을 통한 태아로의 방사성스트론튬 전이를 효과적으로 억제함을 관찰하였다(p<0.01, 표 1).본 실험을 통해서 임신 17일째에 방사성스트론튬에 오염된 임신마우스의 경우 천연무독성 착화제인 카이토산을 장기간 전처치하였을 경우 태반을 통한 태아로의 방사성스트론튬 전이를 억제할 수 있음을 알 수 있었다. It has been reported that chitosan has little genetic toxicity as one of natural and nontoxic chelator and reduces the internal retention of radiostrontium in the mouse. This study is to examine that when water soluble chitosan is provided to the mouse on 17 days of pregnancy before and after radiostrontium contamination, how effectively it can inhibit an acute transfer of radiostrontium to fetus through placenta contaminated. Water soluble chitosan powder is mixed with general food for 60 days and 10%(Group 1) and 1%(Group 2) are provided respectively, and it is observed that the group with radiostrontium contamination on 17 days of pregnancy can inhibit more effectively the transfer of radiostrontium to fetus through placenta than control group with general food and the groups (Group 3, Group 4) with 10% and 1% of chitosan powder respectively after radiostrontium contamination (p<0.01, Table 1). It is found that when the pregnant mouse contaminated by radiostrontium on 17 days of pregnancy is prefed by chitosan, the transfer of radiostrontium to fetus through placenta can be inhibited.

윈도우 기반 응용프로그램 제공 서비스를 위한 Win32 API 메시지 인가 시스템의 개발

김영호,정민아,원용관,Kim, Young-Ho,Jung, Mi-Na,Won, Yong-Gwan 한국정보처리학회 2004 정보처리학회논문지 C : 정보통신,정보보안 Vol.11 No.1

컴퓨터 자원의 대용량화 및 네트워크 속도의 증가로 인하여 사용자가 네트워크를 통해 원격지의 서버에 접속하여 컴퓨터론 사용하는 요구가 증가되었다. 이에 따라 중앙집중형 컴퓨팅을 통한 응용프로그램 제공 서비스도 활성화되었다. 중앙집중형 컴퓨팅 시스템은 중앙의 대용량컴퓨터 시스템에 설치된 응용프로그램을 공유 프로토콜을 통하여 원격 사용자에게 제공하는 응용프로그램 공유 서비스(ASP: Application Service Provision) 시스템 모델이다. 중앙집중형 컴퓨팅 시스템을 통한 응용프로그램 공유 서비스는 기밀성, 가용성 무결성 등의 보안 사항이 반드시 유지되어야 한다. 기존 원격 컴퓨팅인 Telnet, FTP 접속은 단순히 파일 및 데이터의 접근 권한을 제어함으로 보안이 유지된다. 그러나 윈도우 기반 시스템의 경우 다수의 사용자가 통일한 권한을 통해 통일한 응용프로그램을 제공받기 때문에 사용자들 사이에 기밀성 및 무결성을 저해 할 수 있다. 또한 다수의 사용자가 하나의 응용프로그램에 파일열기, 복사, 서식 수정 등의 여러 기능 명령어를 전송하기 때문에 파일 및 데이터 접근 제어만을 통해서는 시스템의 기밀성 유지한 수 없다. 또한 기밀성의 문제는 곧 가용성 및 무결성의 문제로 이어질 수 있다. 본 논문에서는 윈도우 기반 중앙집중형 컴퓨팅 시스템의 응용프로그램 공유 서비스를 지원함에 있어 사용자가 실행하는 Win32 API 메시지 명령어 접근제어 시스템을 제안한다. 제안하는 시스템은 GUI(Graphical User Interface) 기반의 서버에서 사용자가 서버에 접속하여 발생하는 모든 메시지(마우스, 키보드, I/O, etc....)들을 감시한다. 감시된 메시지 기반의 명령어는 미리 설정된 사용자별 보안 정책에 기반 하여 해당 응용프로그램에게 전달 여부가 결정된다. 이러한 메시지 기반 상세 보안을 통해 기밀성 침해의 우려가 있는 메시지 명령어를 차단하고, 기능 명령어 차단에 의한 자원의 기밀성을 해결하였다. The growth of computer resource and network speed has increased requests for the use of remotely located computer systems by connecting through computer networks. This phenomenon has hoisted research activities for application service provision that uses server-based remote computing paradigm. The server-based remote computing paradigm has been developed as the ASP (Application Service Provision) model, which provides remote users through application sharing protocol to application programs. Security requirement such as confidentiality, availability, integrity should be satisfied to provide ASP service using centralized computing system. Existing Telnet or FTP service for a remote computing systems have satisfied security requirement by a simple access control to files and/or data. But windows-based centralized computing system is vulnerable to confidentiality, availability, integrity where many users use the same application program installed in the same computer. In other words, the computing system needs detailed security level for each user different from others, such that only authorized user or group of users can run some specific functional commands for the program. In this paper, we propose windows based centralized computing system that sets security policies for each user for the use of instructions of the application programs, and performs access control to the instructions based on the security policies. The system monitors all user messages which are executed through graphical user interface by the users connecting to the system. Ail Instructions, i.e. messages, for the application program are now passed to authorization process that decides if an Instruction is delivered to the application program based on the pre-defined security polices. This system can be used as security clearance for each user for the shared computing resource as well as shared application programs.

방사광을 이용한 괴타이트에 대한 고압 X - 선 회절연구

김영호,이지은 ( Young Ho Kim,Ze On Yi ) 한국광물학회 1997 광물과 암석 (J.Miner.Soc.Korea) Vol.10 No.2

홍미삼과 홍삼정박의 건열처리가 Fusarium owsporum의 균사 생장과 승홍에 대한 내성에 미치는 영향

김영호,박명한,이종원,Kim, Yeong-Ho,Park, Myeong-Han,Lee, Jong-Won 고려인삼학회 1992 Journal of Ginseng Research Vol.16 No.2

Korean red ginseng and water extract residue of red ginseng roots were treated with dry heat and incorporated in PDA medium to examine the effect of the materials on induced tolerance against mercury chloride and mycelial growth of Fusarium oxysporum. Ginseng residue was not effective in the inducement of tolerance to mercury chloride regardless of dry heat treatment. However, the heat treatment of ginseng and ginseng residues stimulated the mycelial growth of the fungus. The materials responsible for the detoxification appeared to be water-soluble. The stimulation of the fungal mycelial growth on the media by the heat treatment was highest in the water extract of ginseng. Due to the heat treatment, the mycelial growth was also slightly increased in n-hexane and methanol extracts of ginseng, compared with the ginseng fractions without dry heat treatment.

천련자로부터 분리한 Limonoid 성분의 세포독성

김영호,황방연,김세은,김환묵,오구택,노재섭,이경순,이정준,Km, Young-Ho,Hwang, Bang-Yeon,Kim, Se-Eun,Kim, Hwan-Mook,Oh, Goo-Taeg,Ro, Jai-Seup,Lee, Kyong-Soon,Lee, Jung-Joon 대한약학회 1994 약학회지 Vol.38 No.1

The MeOH extract of the fruits of Melia toosendan was selected for futher study by its cytotoxicity and effect on the human breast cancer cell line, MCF-7. The active principle obtained by activity guided fractionation followed by purification gave rise to a needle crystal. The structure was deduced by employing NMR and was determined to be identical with 28-deacetyl sendanin by comparison with published data. This compound induced morphological change of MCF-7 to be rounded with tubule at concentrations between $50\;{\mu}g/ml$ and $0.025\;{\mu}g/ml$. This compound, however, showed strong cytotoxic effect on Hepalclc7 and HepG2, and their $GI_{50}$ on the hepatoma cell lines were $0.238\;{\mu}g/ml$ and $0.805\;{\mu}g/ml$, respectively. Its effect on lymphocyte of mouse was stronger than hepatoma cell lines, and their $ED_{50}$ of polyclonal antibody response was $0.011\;{\mu}g/ml$, and $ED_{50}$ of cell viability was $0.039\;{\mu}g/ml$.

흉강경검사의 임상적 고찰

김영호,조중구,Kim, Yeong-Ho,Jo, Jung-Gu 대한흉부심장혈관외과학회 1984 Journal of Chest Surgery (J Chest Surg) Vol.17 No.3

During a 12-month period, 31 patients underwent diagnostic and therapeutic thoracoscopy for previously undiagnosed thoracic diseases. In all patient, the diagnosis had been unobtainable by the usual diagnostic modalities of thoracentesis, closed pleural biopsy, bronchoscopy, or mediastinoscopy. The patients ranged from 4 years to 84 years old. One procedure was performed for mediastinal mass, 8 for parenchymal lesions, 21 for pleural diseases, and 1 for diaphragmatic disease. A correct diagnosis was obtained by thoracoscopy in 31 procedures for a 90% overall accuracy rate. There was no clinically significant morbidity in this series and no procedure-related mortality. Thoracoscopy, performed under intercostal nerve block and regional anesthesia, has proved to be a very attractive method of the diagnosis of thoracic disease.

개심술후 요도협착 `

김영호,조중구,김공수,Kim, Yeong-Ho,Jo, Jung-Gu,Kim, Gong-Su 대한흉부심장혈관외과학회 1985 Journal of Chest Surgery (J Chest Surg) Vol.18 No.3

In a review of our cases presenting with urethral strictures, we noted a high incidence following open heart surgery. In an attempt to elucidate factors predisposing to the occurrence of urethral stricture, we studied the following data; preoperative laboratory study, aortic clamp time, dosage of heparin and protamine, degree of hypothermia, platelet count, and blood pressure. No significant differences were found between the stricture group and the non-stricture group. Of 33 patients admitted in our hospital with urethral stricture, 8 had suffered after open heart surgery. We believe that the urethral catheter is at least partly responsible for stricture formation. Associated factor, for example urethral ischemia, may be contributory.