기혈양허로 변증한 파킨슨병 환자 증례 보고

김영은,김일화,이재화,이성근,이기상,Kim, Young-Eun,Kim, Il-Wha,Lee, Jae-Hwa,Lee, Seoung-Geun,Lee, Key-Sang 대한한방내과학회 2009 大韓韓方內科學會誌 Vol.30 No.4

Parkinson's disease is a slowly progressive degenerative disorder of the central nervous system. It is characterized by tremor when muscles are at rest, increased muscle tone, slowness of voluntary movements, and difficulty maintaining balance. In oriental medicine, these symptoms are diagnosed as yin(陰)-deficiency of liver and kidney, deficiency of qi(氣) and blood, retention of phlegm(痰), qi-stagnation and blood stasis. In this case, we diagnosed patients as deficiency of qi(氣) and blood type according to symptoms and treated by herbs that strengthen yang and benefiting yin for two weeks, while maintaining existing parkinson's western medication. After treatment, clinical symptoms were improved, while UPDRS (Unified Parkinson's Disease Rating Scale) score was decreased. These cases suggest that oriental medicine therapy maybe effective in the treatment of Parkinson's disease.

대학생의 데이트 폭력 관련 전문적 도움추구 태도 영향 요인

김영은,윤미선,이현정,Kim, Youngeun,Yoon, Mi-Sun,Lee, Hyun-Jung 중소기업융합학회 2021 융합정보논문지 Vol.11 No.10

본 연구는 최근 데이트폭력의 심각성이 부각되고 있는 추세에 부합하여 대학생의 데이트폭력 인식을 포함한 다양한 영향 요인과 전문적 도움추구 태도와의 관계를 통합적으로 탐색하고 그 상대적 설명력의 크기를 살펴보는 것을 목적으로 하였다. 충청권 소재 1개 대학교의 508명의 대학생이 자기보고식 질문지에 응답하였고 수집된 자료는 SPSS 22.0을 사용하여 상관관계 및 회귀분석을 하였다. 연구 결과를 요약하면 다음과 같다. 심리적 불편감 노출 및 상담에 대한 유용성기대는 전문적 도움추구 태도와 정적 상관관계를 보였고, 연인 간의 비합리적 폭력인식과 지각된 사회적지지, 상담에 대한 위험기대, 및 도움 요청 장애 요인은 전문적 도움추구 태도와 부적 관계로 나타났다. 위 변인들의 총 설명량은 46.1% 으로 상담에 대한 유용성기대가 가장 큰 설명력을 지니고 위험기대와 도움요청 장애요인이 그 다음으로 큰 설명력을 가지는 것으로 나타났다. 본 연구는 데이트폭력 연구 주제의 확장 및 대학생의 전문적 도움 서비스 활용 활성화를 위한 방향성 제시를 했다는 의의를 지닌다. The purpose of this study was to explore relations between college students' dating violence perception and professional help-seeking attitudes and other factors that affect professional help-seeking attitude and their effect size. 508 students from a college in Chungcheong region responed to self-report questionnaires. Data were analyzed by Partial correlation coefficients and regression analysis using the SPSS 22.0 program. As a resullt, self-disclosure and anticipated utility on self-disclosure had positively effect on professional help-seeking attitudes. In contrast, violence perception, perceived social support, risk on self-disclosure and barriers to seeking help had negative effect on professional help-seeking attitudes. The total effect size was 46.1%, and the effect size of anticipated utility on self-disclosure was the largest, followed by risk on self-disclosure and barriers to seeking help. This study contributed to the broading of dating violence research topics and provided the directions to the endavour to increase the use of professional services among college students

홍차 흑효모 발효물의 항산화, 항주름 및 미백효과

김영은,여민아,한정희,이정민,정석균,정현아,김선화,이지언,Kim, Young Eun,Yeo, Min-A,Han, Jeung Hi,Lee, Jung Min,Jung, Suk Kyun,Jeong, Hyeon Ah,Kim, Seon Hwa,Lee, Ji Ean 대한화장품학회 2017 대한화장품학회지 Vol.43 No.3

본 연구에서는 홍차 흑효모 발효물의 기능성화장품 소재로서의 가능성을 규명하고자 항산화, 미백, 항주름 효능을 확인하였다. 홍차 흑효모 발효물의 결과는 홍차 추출물, 흑효모 발효물과 비교하여 나타내었다. 항산화능 평가 결과 홍차 흑효모 발효물은 흑효모 발효물보다 뛰어난 DPPH 라디칼 소거능을 나타냈으며 홍차 추출물과는 유사한 결과를 나타냈다. 과산화수소로 유도된 세포 내 활성산소종 실험에서도 홍차 흑효모 발효물은 유의적 제거 활성을 보였다. 또한 홍차 흑효모 발효물은 tyrosinase 저해 활성을 보이며 Melan-a, B16F10 세포를 이용한 멜라닌 형성이 억제됨을 확인하였다. 항주름 효능 확인을 위해 콜라겐 분해 효소(MMP-1)를 측정한 결과 홍차 흑효모 발효물이 홍차 추출물, 흑효모 발효물보다 높은 항주름 활성을 보였다. 본 연구 결과를 통해 홍차 흑효모 발효물은 항산화능 효능을 보이며 미백, 항주름 효능을 보아 기능성 화장품 소재로 개발될 수 있음을 확인하였다. In this study, anti-oxidant, whitening and anti-aging effects were investigated the possibility of fermented black tea yeast as a functional cosmetic material. The results of Aureobasidium pullulans (A. pullulans) ferment of black tea (AFBT) were compared with black tea (BT) and A. pullulans ferment (AF). The antioxidant activity of the AFBT product showed DPPH radical scavenging ability higher than that of AF. In the intracellular ROS experiments induced by hydrogen peroxide, the AFBT showed significant decreased activity. In addition, AFBT showed tyrosinase inhibitory activity and inhibited the formation of melanin using Melan-a and B16F10 cells. Through suppression of MMP-1 expression, the AFBT showed higher anti-wrinkle activity than BT and AF. From the results of this study, it was confirmed that AFBT showed antioxidant activity and was developed as a functional cosmetic material based on whitening and anti-wrinkle efficacies.

유한요소 모델을 이용한 인간 뇌의 미만성 부상에 대한 해석

김영은,남대훈,Kim, Yeong-Eun,Nam, Dae-Hun 대한의용생체공학회 1998 의공학회지 Vol.19 No.6

가속도 변화에 따른 뇌의 미만성 부상을 해석하기 위하여 성인 및 2세, 6세아의 머리 부분에 대한 유한 요소 모델을 개발하였다. 외력은 최대값이 200g인 삼각형 형태의 가속도를 가하였으며, 가속도의 방향, 지속시간에 따른 변화를 해석하였다. 가속도 변화에 따라 발생되는 뇌내의 전단력 분포는 뇌간, 뇌교 및 중뇌등 신경조직이 밀집된 곳에서 크게 발생되어 이곳에서 미만형 부상이 발생할 확률이 높음을 알 수 있었으며, 특히 6세아 모델의 경우 뇌간에서의 최대 전단력이 굴전 형태의 회전가속도 받았을 때 가장 크게 나타나는 결과를 보여 개발된 모델이 임상결과와 일치함을 보여주고 있었다. 가속도 지속 시간이 길어짐에 따라 뇌내에 발생되는 압력 및 최대 전단력의 크기가 증대되고 있었으며, 유아모델의 경우 성인모델에 비하여 가속도 방향과 관계없이 낮은 압력이 발생하였지만 발생압력이 감소하지 않고 지속되는 현상을 보이고 있었다. 그리고 각 가속도에 의한 미만성 부상을 예방하기 위한 안전지수로는 현재 탑승자의 안전 설계에 활용되고 있는 HIC보다는 최대 전단응력이 더 적절한 부상 예측인자임을 알 수 있었다. To anlyze the diffuse axonal injury of the human brain, 3-D finite element models of the adult, two and three years child were developed. Triangular type acceleration which had its maximum value 200g was applied to investigate the effects of acceleration direction and duration time. The pattern of high shear stress generated at the brain stem, pones and midbrain was similar to the pattern of DAI seen in the clinical observation, especially high maximum shear stress was detected in the brain stem of the six year old child model under flexional acceleration. As the duration of acceleration increased generated pressure and maximum shear stress also increased. For the children's model relatively small pressure was generated regardless of the acceleration direction and continued much longer compared with adult's model. From this analysis maximum shear stress was revealed more proper indicator to predict DAI compared to HIC in case of angular acceleration loading.

성인 뇌와 유아 뇌 모델의 진동 특성 해석

김영은,유진환,Kim, Yeong-Eun,Yu, Jin-Hwan 대한의용생체공학회 1998 의공학회지 Vol.19 No.6

머리(뇌 포함) 부분에 대한 3차원 유한 요소 모델을 이용하여 진동 방향에 따른 응답특성을 해석하였다. 해석 결과 성인의 뇌는 9Hz, 14Hz에서 고유 진동수를 나타내었으며 유아의 뇌에서는 2Hz, 3Hz에서 고유 진동수가 계산되었다. 진동 방향에 관계없이 각 진동에 대해 계산된 가속도를 제외한 전단력, 압력, von-Mises 값의 최대, 최소값의 비는 성인에 비해 유아모델에서 더 크게 나타났다. 각 고유 진동수에서 성인모델은 14Hz에서 상지 중추보다 하지 중추를 관장하는 뇌의 영역에서 보다 큰 응력이 계산되어 상지에 의한 오동작 보다 하지에 의한 오동작을 일으킬 가능성이 더 큼을 유추할 수 있었다. Using three dimensional finite element model of the human brain, vibratory characteristics of the human brain according to vibratory direction was analyzed. From this analysis 9, 14Hz and 2, 3Hz natural frequencies were calculated for adult's and baby's brain model respectively. Regardless of the vibratory direction relatively high shear stress, pressure and von Mises stress variation except acceleration were detected in the baby brain model. At each natural frequencies, adult's model showed relatively high stress level in the region of lower limb control area compared with upper limb control area at 14Hz natural frequency.

녹용의 약효 성분에 관한 연구 ( Ⅴ ) 녹용 및 판토크린의 당지질과 인지질의 조성에 관하여

김영은,임동구,신승언 ( Young Eun Kim,Dong Koo Lim,Seung Uon Shin ) 생화학분자생물학회 1977 BMB Reports Vol.10 No.3

The lipid soluble fraction of Antler velvet layer (cervus nippon taiouanous) was extracted and compared to that of pantocrin (ethanol preparation of Antler, commercially available). Lipid soluble components (801㎎/24.5g from Antler velvet ayer and 979.1㎎/143㎖ from pantocrin) were fractionated by gel filteration using a sephadex CT-25 into neutral lipids, sulfatides, cerebrosides, phospholipids and non-lipid components (lipoprotein and peptide). A silicic acid and a DEAE-sephadex A-25 column chromatography were followed from refractionation and purification. Each fraction was identified by thin layer chromatography using standard materials. Typical lipid soluble fraction of Antler contained 65.3% neutral lipid, 5.2% glycolipid, 12.9% phospholipid, 6.1% ganglioside and 2.9% non-lipid components. The glycolipid was composed of 63.5% cerebroside and 36.5% sulfatide. The cerebroside fraction of the Antler velvet layer showed four spots while pantocrin gave only one spot on a thin layer chromatogram. Among the four spots of Antler cerebroside two had almost same migration with standard but two had quite different mobility compared to the standard. The latter fractions were hydrolyzed by 0.025M methanolic HCl in order to desulfate. The two unknown materials were identified as sulfatides by the method of IR spectroscopy and thin layer chromatography. By two dimensional thin layer chroma tography, it was found that phospholipid of thr. Antler velvet layer were composed of phosphatidylethanolamine, sphingomyelin, phosphatidylcholine, lysophospahtidylethanolamine, and lysophosphatidylcholine and phosphatidic acid. On the other hand pantocrin contained phosphatidylcholine and sphingomyelin as phospholipids.

녹용의 약효 성분에 관한 연구 ( Ⅳ ) 녹용 벨벳층의 프로스타그란딘의 검출

김영은,이승기,이명희 ( Young Eun Kim,Seung Ki Lee,Myoung Hee Lee ) 생화학분자생물학회 1977 BMB Reports Vol.10 No.1

The fresh antler was cut, frozen and mechanically separated into spongy bone layer and velvet layer. The prostaglandin-like components were extracted from antler velvet layer and qualitatively analyzed by three different methods: thin layer chromatography, ultra-violet spectroscopy after conversion to PGB series by alkaline treatment, and gas liquid chromatography. The prostaglandins detected were PGE₂, 15-epi-PGE₁, PGF_(1α), PGF_(1β). The experiments indicated that the petroleum ether fraction contained mostly PGE series and that the ethylether fraction contained PGF series. One major prostaglandin-like components of antler velvet layer was found to be the main compound in the petroleum ether extract. The structure of this compound is still unknown but the gas liquid chromatography data suggest that it is a hydroxy fatty acid.

실험적 해석을 위한 머리-목 형태의 더미 개발

김영은,남대훈,고창훈,Kim, Yeong-Eun,Nam, Dae-Hun,Koh, Chang-Hun 대한기계학회 1997 大韓機械學會論文集A Vol.21 No.7

A head-neck complex dummy, for measuring brain pressure and reaction force in the cervical spine was developed for experimental study related in injury mechanism. Dummy comprised aluminium-casted head with water filled cavity for simulating brain and mechanical neck assembled with six motion segments. Several kinds of experiments (compression, bending, cyclic modulus, relaxation and constant velocity profile) for the developed mechanical neck showed that this neck model is biomechanically reliable compared with in-vitro test results. As an application of developed head-neck complex dummy, shock absorbing properties of protective helmet was chosen. The experiments showed that the maximum pressure increment of brain after impact was tolerable compared with the guide line for mild brain injury pressure (25psi). Constrast to this results, the reaction force in the neck was high enough to produce failure in the cervical spine.

단어 단위 접근법을 이용한 음운장애 아동과 정상 아동의 음운 분석

김영은,최성일,박상희,Kim, Young-Eun,Choi, Sung-Il,Park, Sang-Hee 한국음성학회 2006 음성과학 Vol.13 No.4

Recently, many researchers have been interested in children with phonological disorders. The purpose of this study was to examine those children in comparison with normal children and to find better assessment criteria of the whole-word approach. Three children with phonological disorders and three normal children of 5 to 7 years old participated in the picture description tasks. Results of this study were as follows: there was a significant difference in the whole-word assessment between normal and phonological disorder children. Such criteria as whole-word correctness, whole-word complexity, whole-word intelligibility proved to be good for diagnosing children's phonological disorders. Further studies would be desirable to apply the approach to more children of various age groups.

표준 요 시료 중 Oxalate의 측정을 위한 FT-NIR 분광기의 유용성 검정

김영은,홍수형,김정완,이종영,Kim, Yeong-Eun,Hong, Su-Hyung,Kim, Jung-Wan,Lee, Jong-Young 대한예방의학회 2006 예방의학회지 Vol.39 No.2

Objectives : The determination of oxalate in urine is required for the diagnosis and treatment of primary hyperoxaluria, idiopathic stone disease and various intestinal diseases. We examined the possibility of using Fourier transform near-infrared (FT-NIR) spectroscopy analysis to quantitate urinary oxalate. The practical advantages of this method include ease of the sample preparation and operation technique, the absence of sample pre-treatments, rapid determination and noninvasiveness. Methods : The range of oxalate concentration in standard urine solutions was $0-221mg/{\ell}$. These 80 different samples were scanned in the region of 780-1,300 nm with a 0.5 nm data interval by a Spectrum One NTS FT-NIR spectrometer. PCR, PLSR and MLR regression models were used to calculate and evaluate the calibration equation. Results : The PCR and PLSR calibration models were obtained from the spectral data and they are exactly same. The standard error of estimation (SEE) and the % variance were $10.34mg/{\ell}$ and 97.86%, respectively. After full cross validation of this model, the standard error of estimation was $5,287mg/{\ell}$, which was much smaller than that of the pre-validation. Furthermore, the MCC (multiple correlation coefficient) was 0.998, which was compatible with the 0.923 or 0.999 obtained from the previous enzymatic methods. Conclusions : These results showed that FT-NIR spectroscopy can be used for rapid determination of the concentration of oxalate in human urine samples.