http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 김연향(Yeon Hyang Kim) (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
-
Big-h3내 RGD-motif의 유전자 변이 유도에 의한 유방암 세포의 성장 및 이동성에 미치는 영향
김연향(Yeon-Hyang Kim) 산업기술교육훈련학회 2019 산업기술연구논문지 (JITR) Vol.24 No.4
The extracellular matrix (ECM) plays an important role in cellular migration associated with pathological conditions, such as inflammation and cancer. Big-h3 (also known as transforming growth factor-β-induced protein (TGFBI), beta ig-h3, TGF-b-induced protein h3, keratoepithelin, and RGD-CAP) is an extracellular secretory protein and includes the YH and RGD motifs, which interact with the ECM. In this study, big-h3 and a big-h3 fragment (big-h3-f, 182 amino acids) containing the RGD-motif were cloned. To determine whether the RGD-motif affects cell growth, the aspartic acid residue (D) in the RGD motif of wild-type big-h3 or big-h3-f was mutated to a glutamic acid (E) via site-directed mutagenesis, thereby yielding a RGE-motif containing mutant protein. Big-h3, big-h3-f, mutant big-h3, or mutant big-h3-f were transfected into the human breast cancer cell line MCF-7 and their expression was confirmed using immunoblotting. Our results showed that wild type big-h3 and big-h3-f containing the RGD-motif led to increased cell growth and invasion, compared to the corresponding mutants with the RGE-motif. Therefore, the RGD-motif in big-h3 possibly plays a major role in cell growth and regulation of invasion, in the context of cell-ECM interaction.
-
유해활성산소 및 유해산화질소가 유발하는 핵과 소포체의 변화 분석
김연향(Yeon Hyang Kim),정은영(Eun Young Jung),정지윤(Ji Yun Jung),정성오(Sung Oh Chung) 산업기술교육훈련학회 2014 산업기술연구논문지 (JITR) Vol.19 No.3
To investigate cross-talk between endoplasmic reticulum(ER) stress and oxidative stress in astroglioma, the cells were treated with hydrogen peroxide, DETA-NONOate and thapsigargin to raise intracellular levels of oxidative stress and ER stress, respectively. Cell viability was decreased in a dose-dependent manner in hydrogen peroxide- and DETA-NONOate - treated cells. Hydrogen peroxide, DETA-NONOate and thapsigargin also induced DNA fragmentation in these cells as indicated by terminal deoxynucleotidyl transferase-med iated dUTP nick end labeling. Hydrogen peroxide, DETA-NONOate and thapsigargin induced intracellular Ca2+ increase and perturbed Ca2+ homeostasis in endoplasmic reticulum(ER). Likewise, hydrogen peroxide, DETA-NONOate and thapsigargin also increased the expressions of ER stress- related molecules, IREl-a. The increase of IRE1 -a expression is coincided with those of p-JNK up on the treatment with hydrogen peroxide, DETA-NONOate, and thapsigargin in cells. These finding indicate that the increase of expression of IREl-a activating JNK pathway is induced during hydrogen peroxide, DETA-NONOate and thapsigargin - mediated cell death. Therefore, we conclude that oxidative stress including reactive oxygen species and nitric oxide cause nucleus and endoplasmic reticulum(ER) dysfunction and ER-related signals to occur in human astroglioma cells.
-
김현주 ( Hyun Ju Kim ),이여명 ( Yeo Myeong Lee ),김연향 ( Yeon Hyang Kim ),원선임 ( Sun Im Won ),최성아 ( Sung A Choi ),최신욱 ( Shin Wook Choi ) 대한화장품학회 2009 대한화장품학회지 Vol.35 No.2
후박추출물은 중국 원산인 당후박에서 제조되며, 항산화 및 항염증 효과로 알려져 있다. 후박의 성분으로는 폴레페놀인 honokiol 및 magnolol 등이 알려져 있다. 이 연구에서, 우리는 후박추출물이 triglyceride (TG)의 축척을 감소시킴으로써 지방세포 분화를 억제한다는 표면적인 결과를 얻었다. 또한, 후박 추출물이 hormone sensitive lipase (HSL) 단백질 수준을 증가시키고 adipocyte에 specific한 transcription factors로 peroxisome proliferator activated receptor (PPAR)-γ의 단백질과 mRNA 수준을 억제하였다. 결과적으로, 후박추출물이 항비만제로서 adipogenic transcription factor와 그들의 특이적인 유전자의 발현을 억제함으로써 지방세포 분화를 억제한다고 사료된다. Magnolia extract, prepared from the Chinese herb Magnolia officinalis, is known for its potent anti-oxidative and anti-inflammatory effects. In this report, we showed that Magnolia extract inhibits adipocyte differentiation, as evidenced by reduced triglyceride (TG) accumulation. Also, Magnolia extract increased hormone sensitive lipase (HSL) protein level, and decreased the adipogenic transcription factor peroxisome proliferator activated receptor (PPAR)-γ protein and their corresponding mRNA. Our results suggest a potential apllication of Magnolia extract as anti-obesity agents inhibits adipocyte differentiation through the down-regulation of adipogenic transcription factors and other adipocyte-specific genes.
-
토양으로부터 α- glucosidase 저해제 생산균의 분리 및 특성
오광근(Kwang Keun Oh),김연향(Yeon Hyang Kim),박종일(Jong Il Park),문무상(Moo Sang Moon) 산업기술교육훈련학회 2018 산업기술연구논문지 (JITR) Vol.23 No.1
The isolation, characterization and culture conditions of bacteria producing α- glucosidase inhibitor from soil and Korean fermented foods were investigated. As a result of screening for the α- glucosidase inhibitory activity on 100 bacteria isolated, strain N1- 5 with an inhibition ratio of 71.6% against α- glucosidase activity compared with the inhibition ratio of acarbose(25μg/ml) of 49.1%, was finally selected. This strain was identified by Vitek2 compact system and 16S rDNA sequencing, and named Bacillus subtilis N1- 5. The optimum carbon source of B. subtilis N1- 5 for the production of α- glucosidase inhibitor was 1% glucose and the optimum nitrogen source was 0.6% polypeptone. The production of α- glucosidase inhibitor by B. subtilis N1- 5 showed a typical primary metabolite production pattern in the 5- liter jar fermenter using optimized media. The partially purified α- glucosidase inhibitor by B. subtilis N1- 5 was maintained at pH 2.0~3.0 without significantly changing α- glucosidase inhibitory activity. From the above results, it was confirmed that B. subtilis N1- 5 isolated from soil produces a relatively stable α- glucosidase inhibitor similar to acarbose, a therapeutic agent for diabetes.
ScienceON연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
