태아횡위를 동반한 만삭 임신자궁의 염전 1 예

김성훈(Seong Hoon Kim),진규섭(Kyu Seob Jin),이상욱(Sang Uk Yi),이선경(Seon Kyung Lee),허주엽(Ju Yeop Huh),김승보(Sung Bo Kim) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.2

Uterine torsion is defined as the mtation of more than 45。 around the long axis of the uterus or a rotation which is severe enough to produce symptoms. Torsion of the human pregnant uterus is a very rare complication. The diagnosis is problematic and is often made during a cesrean section due to inhibited labor. Torsion of the human pregnant uterus can be caused by myoma or other uterine abnormalities, ovarian cysts, adhesions, abnormal fetal presentations, or fetal abnormalities ( though no cause can be found in about 20% of cases ). Uterine torsion produces symptoms of varying severity depending on the degree of rotation, and duration of the pregnancy. High mortality rates for both mother and infant have been reported. We experienced one case of the torsion of term pregnant uterus and report this case with a brief review of the concerned literature.

침윤성 자궁경부암에서 Thrombospondin-1 mRNA 발현의 신생혈관형성과 임상병리학적 인자와의 상관관계

김여홍 ( Kim Yeo Hong ),이선경 ( Lee Seon Gyeong ),지성길 ( Ji Seong Gil ),이주희 ( Lee Ju Hui ),김승보 ( Kim Seung Bo ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.11

목적 : 자궁경부암에서 전이과정의 조절은 다른 암과 마찬가지로 명확히 정의되어 있지 않다. 자궁경부암에서 신생혈관 형성이 임상적 결과에 강한 영향을 미치며, 암증식 및 전이과정에서 필수적이다. Thrombospondin-1 (TSP-1)은 여러 가지 암에서 발견되며 종양성장과정에서 혈관 형성 및 전이를 억제하는 인자로 알려져 있다. 본 연구에서는 자궁경부암 환자에서 TSP-1 mRNA 발현과 자궁경부암의 임상병리학적 인자 및 미세혈관수 등과의 연관성을 Objective : Acquisition of a proangiogenic environment is essential to the cervical cancer growth, invasion and metastasis, and the angiogenic phenotype in cervical cancer is strongly associated with clinical outcome. However, the regulation of the metast

복막결핵을 동반한 양측 낭성 기형종의 파열로 인한 급성 복막염 1 예

박성재(Seong Jae Park),정민형(Min Hyung Chung),허주엽(Joo Yup Huh),김봉희(Bong Hee Kim),김승보(Seung Bo Kim) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.10

Rupture of teratoma is rare. Ovary cystic teratoma was occurred 95% in benign ovary tumor, but chemical peritonitis arising from ruptured cystic teratoma is a scarce case. Peritoneal tuberculosis is also rare disease nowadays. It causes easily fatigue, abdominal distension, intermittent abdominal pain and ascities insidiously. We have recently experienced a case of chemical peritonits from ruptured both ovary cystic teratoma of ovary with superimposed peritoneal tuberculosis in 44 year-old woman and report our scarce case with a brief review of the literature.

자궁경부암에서의 COX-2 와 VEGF 발현에 관한 연구

박성재 ( Park Seong Jae ),이선경 ( Lee Seon Gyeong ),이주희 ( Lee Ju Hui ),김승보 ( Kim Seung Bo ) 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.5

목적 : 자궁 경부암을 포함하여 여러 악성 종양에서 cyclooxygenase-2 (COX-2)의 과발현이 종양형성의 단계에 관련 있다고 보고되고 있다. COX는 prostaglandin형성에 관여하는 효소인데 두 도가지 이성체가 알려져 있다. 평소에는 정상조직에서는 거의 발현되지 않는 혈관신생과 관련하여 종양형성에 관여하는 COX-2와 과발현의 유발은 조직의 저산소증에 의해 조절되는 것으로 알려져 있으며, 이런 저산소증은 vascular endothelial growth factor (VEGF)의 발현을 유발한다고 한다. 이에 저자들은 자궁경부암중 침윤성암 25예, 미세침윤성암 7예, 그리고 상피내암종 9예의 조직에서 COX-2 및 VEGF의 과발현과 미세혈관밀도의 상관관계를 알아보고자 하였다. 연구 방법 : 1999년 1월부터 2001년 12월까지 경희대학교 산부인과에서 자궁경부암으로 진단받은 41예와 정상자궁경부 조직 4예를 대상으로 COX-2 단클론성 항체와 Rabbit anti-human VEGF 항체를 이용하여 면역조직 화학 염색을 시행하였다. 또한 factor Ⅷ 항체를 이용하여 미세혈관밀도를 측정하였다. COX-2와 VEGF의 면역조직화학적 염색결과는 면역반응의 염색 강도와 염색된 세포의 수에 따라 음성 (0), 약양성 (light staining, 1), 중간양성 (moderate staining, 2), 강양성 (heavy staining, 3)으로 반정량적 판독을 하였다. 결과 : COX-2 면역반응은 상피세포 기저막과 암 침윤부위가 특히 강하게 발현되는 것을 볼 수 있었다. 그리고 상피내암종, 미세침윤성 자궁경부암, 침윤성 자궁경부암으로 갈수록 강양성의 반응도가 증가하는 것을 관찰할 수 있었고 (p=0.006), VEGF 면역반응 역시 암이 진행할수록 양성의 빈도가 증가하는 경향을 확인하였다 (p=0.079). 이들 COX-2 면역반응과 VEGF 면역반응은 상관관계가 있는 것으로 확인되었다 (r_(s)=0.686, p<0.01). 또한 이들의 면역반응은 미세혈관 밀도와 상관관계를 보이는 것으로 관찰되었다. 결론 : COX-2와 VEGF의 발현이 자궁경부 종양의 발생초기 단계에 중요한 상호역할을 하는 것으로 사료된다. Objective : The purpose of this study was to know the potential of growth, invasion and metastasis of uterine cervical cancer associated with neovascularization and to investigate whether the enhanced COX-2 (Cyclooxygenase-2) and VEGF (Vascular Endothelial Growth Factor) expression is showed through CIS to invasive cervical cancer and to examine that COX-2 and VEGF expressions are associated with prognostically worse pathological variables and had a direct correlation with tumor MVD (microvessel density). Methods : From January 1999 to December 2001, 45 paraffin-embedded cervical specimens were obtained by surgical resection in the Kyung Hee University Medical Center. The study group included 25 cases in invasive squamous carcinomas, 7 cases in microinvasive carcinoma, 9 cases in carcinoma in situ, 4 cases in the benign uterine diseases. All tissues were subjected to immunohistochemical staining for COX-2, VEGF and microvessel density (MVD), and immunostaining was scored using a four-point scale with (0) indicating no expression, (1) light staining, (2) moderate staining, (3) heavy staining. Results : As the cervical tumorigenesis progressed, there was a significant increase of MVC (microvessel count) and COX-2 expression. There is a significant correlation between COX-2 and VEGF expression (r_(s)=0.686, p<0.01) in cervical neoplasms. There was no correlation between COX-2, VEGF expression and clinicopathologic factors in cervical cancer. Conclusion : These findings provide evidence that the expression of COX-2 and VEGF is involved in the promotion of angiogenesis in cervical neoplasms and plays an important role in early invasion.

초등학교 중학교 디자인교육과정 개발 연구

정향진 ( Hyang Jin Jung ),김현수 ( Hyun Soo Kim ),김승보 ( Seong Bo Kim ),나현미 ( Hyun Mi Rha ) 커뮤니케이션디자인학회(구 시각디자인학회) 2011 커뮤니케이션 디자인학연구 Vol.35 No.-

Design issue is being magnified to judge a national competitiveness as a main factor in the 21st century. From 2010 Seoul Metropolitan Office of Education sets up Design curriculum as an optional subject on the revised 7th education curriculum in primary and secondary school, that avoids painting centered art education and it is expected for the students to increase an overall thinking ability through the creative Design education. Depends on this current trends and demands, from June through end of Oct. 2009 outstanding national practical projects; A study on the development of primary and 「secondary school Design education goals and contents system and A study on primary and」「secondary school teacher`s program development」 had been completed under the vision for a design-oriented Korea. As an following up project on the two proceedings, this study focuses on the establishment of Design education values in the views of social, cultural and educational aspects. This project covers a primary and secondary Design curriculum in order to build up an institutional framework for the Design performance standard, test criteria development and Design curriculum reorganization and operation as an mandatory course subject.

선행 제왕절개술 후 질식 분만: 성공의 예측인자들

동서연 ( Seo Yun Tong ),김여홍 ( Yeo Hong Kim ),최영준 ( Young Jun Choi ),김소라 ( So Ra Kim ),이보연 ( Bo Yon Lee ),이선경 ( Seon Kyung Lee ),허주엽 ( Chu Yeop Huh ),김승보 ( Seong Bo Kim ) 대한주산의학회 2003 Perinatology Vol.14 No.4

목적: 선행제왕절개 후 질식분만을 시도한 산모들 중 성공군과 실패군을 비교하여 성공을 예측할 수 있는 인자를 알아보고자 하였다. 방법: 1997년 3월부터 2003년 2월까지 경희대학교 의과대학 산부인과에서 선행제왕절개 후 질식분만을 위하여 내원한 환자중 질식분만의 금기가 아닌 경우 위험요소 등에 대하여 충분히 상의한 후 분만을 시도한 382명의 환자를 대상으로 하였다. 대상군의 의무기록을 통하여 분만당시 산모의 나이, 분만력, 제태연령, 신생아 몸무게, 입원시 자궁경부 상태 및 Bishop score, 분만의 진행과정, 결과 및 합병증을 조사하여 성공군과 실패군의 차이를 비교분석 하였다. 결과: 292(76.5%)명이 질식분만에 성공하였다. 여러 인자 중 태아의 몸무게(odds ratio, 0.2 ; 95% confidence interval (CI), 0.1-0.4), 입원시 Bishop score(odds ratio, 1.8 ; 95% CI, 1.5-2.1), 질식분만의 과거력(odds ratio, 3.6 ; 95% CI, 1.1-11.6)이 VBAC의 성공과 관련이 있었다. 또한 선행제왕절개시 적응증이 태위이상인 경우에 성공률이 높은 것으로 나타났다(odds ratio, 3.2 ; 95% CI, 1.2~8.6). 입원시 Bishop score로 VBAC의 성공을 예측할 수 있는 지 알아보기 위하여 receiver-operating characteristic curve 분석을 시행하였고, 5점을 cut-off로 하였을 때 47.4%의 민감도와 87.6%의 특이도를 보였다(AUC, 0.765 ; p<0.05). 결론: 출생체중, 질식분만의 과거력, 입원시 Bishop score가 선행제왕절개 후 질식분만을 고려하는 경우 성공의 예측인자로서 유용하게 사용되어 질 수 있을 것이다. Objective: The purpose of this report is to determine the predictable factors for successful vaginal birth after cesarean (VBAC). Methods: In this study, 382 women who attempted VBAC at Kyung-Hee university hospital were included. The medical records of them were reviewed retrospectively. Informations was collected about demographics, medical history, obstetric history, neonatal birth weight, complications, treatment, and outcome of the index pregnancy. Results: The overall success rate was 76.5% (292 cases). Among variables, neonatal birth weight (odds ratio, 0.2; 95% confidence interval, 0.1-0.4), Bishop score at admission (odds ratio, 1.8; 95% confidence interval, 1.5-2.1 ; p<0.05), and history of vaginal delivery (odds ratio, 3.6 ; 95% confidence interval, 1.1-11.6 ; p<0.05) were significant. The Bishop score showed most significant relationship with successful VBAC. The Bishop score 5 at admission had a sensitivity of 47.4% and a specificity of 87.6% in the prediction of successful VBAC. Malpresentation as indications of previous cesarean delivery showed more tendency to succeed than others. Conclusion: Several factors such as birth weight, Bishop score at admission, and history of vaginal delivery may be used to predict the success of VBAC.

자궁경부암 환자의 임상병리학적 분석 및 예후에 관한 연구

임명철 ( Myong Cheol Lim ),이선경 ( Seon Kyung Lee ),박성재 ( Seong Jae Park ),김승보 ( Seung Bo Kim ) 대한산부인과학회 2005 Obstetrics & Gynecology Science Vol.48 No.2

목적: 자궁경부암은 최근 조기 진단 및 치료로 생존율이 점차 증가하고 있으나 여전히 높은 발생 빈도를 보이는 여성암이라 할 수 있다. 이번 연구를 통해 임상적 특징과 인구통계학적 분석을 하고, 5년 생존률에 영향을 주는 임상적 예후인자를 확인하여 향후 자궁경부암 환자의 치료에 도움을 주고자 하였다. 연구 방법: 1984부터 1998년까지 경희대학교 산부인과에서 자궁경부암으로 진단받고 추적관찰이 가능하였던 445명을 대상으로 후향적으로 FIGO stage Objective: The object of this retrospective study was to evaluate the clinical characteristics and prognosis of invasive uterine cervical cancer. Methods: 445 evaluable patients with invasive cancer of the cervix were treated at Kyung Hee Medical Center f

SV40 T 항원의 온도조건부 변이형 유전자가 포함된 Amphotropic Retrovirus 에 의한 사람 태아 간세포의 불멸화

이정일(Joung Il Lee),동석호(Seok Ho Dong),김효종(Hyo Jong Kim),김병호(Byung Ho Kim),장영운(Young Woon Chang),장린(Rin Chang),성세라(Se Ra Seong),박재경(Jae Kyung Park),김승보(Seung Bo Kim),이상목(Sang Mok Lee) 대한내과학회 1999 대한내과학회지 Vol.57 No.1

N/A Human cells are almost never spontaneously immortalized in vitro. We tried to immortalize human fetal hepatocytes (h-FH) and evaluate the differentiational status and its change. Methods : Hepatocytes were isolated from a liver fragment of 20 week old fetus and infected with amphotropic recombinant retrovirus containing a temperature- sensitive mutant of SV40 large T antigen and neomycin phosphotransferase gene. G418 resistant colonies were cloned and expanded. The cells which were able to divide more than 30 times were used to analyze various functions. Results : The immortalization rate was 3.3 x 10-8 and two cell lines (C11, D21) were established. C11-60, C11-80, D21-30 and D21-60 (suffix number means the cell division counts) were evaluated. D21-30 was thougt to be imcompletely immortalized because a considerable portion of cells died during culture. The morphology was similar to that of epithelial cells except for D21-30 which looked like fibroblast. The cells grew rapidly at 33oC but stopped growing at 39oC. T antigen and p53 was expressed at 33oC but disappeared at 39oC, which suggest that T antigen binds to p53. Chromosomal changes were so marked that it was impossible to discriminate exact number. Albumin was secreted as about 1/10 as that of h-FH, but alpha-fetoprotein secretion stopped after immortalization. Telomerase was activated in both cell lines except for the incompletely immortalized cells D21-30. Telomere was elongated in competely immortalized cell lines, but it was rather shortened in D21-30 compared to that of h-FH. Macroscopic colonies did not develop in soft agar assay. Conclusions : We successfully immortalized human fetal hepatocytes. Although the cells are not likely to have oncogenicity, the functions are not so good, possibly due to marked chromosomal changes which are thought to occur before telomerase is activated during immortalization step.

임신성 고혈압 환자에서의 혈액 응고계의 변화

정현수 ( Hyun Soo Chung ),이선경 ( Seon Kyung Lee ),김승보 ( Seong Bo Kim ),오보훈 ( Bo Hun Oh ),이재현 ( Jae Hyun Lee ) 대한주산의학회 1991 大韓周産醫學會雜誌 Vol.2 No.2