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김슬기,박성영 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1
In this study, we utilized a boronic acid functionalized fluorescent carbon dot (FCD), which has small size, excellent photoluminescent properties, and high biocompatibility. Next, FCD was conjugated with a fluorescent probe (DYE) that caused quenching via electron-receiving ability through the Förster resonance energy transfer (FRET) phenomenon. Here, FCD-DYE could selectively bind to diol group of the bacterial cell surface by forming boronate ester bond that can be used for the detection of bacteria. In the presence of bacteria, DYE is released from FCD, replaced by diol groups on bacteria cell via generating new cyclic ester bonds with FCD, resulting in fluorescent recovery. The system is not only relatively inexpensive and simple, but also become a powerful biosensor that exhibits high sensitivity and affinity to detect bacteria. ** This work was supported by Korea National University of Transportation in 2019.
김슬기,박성영 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0
In this work, we reported on-off switchable imaging using dual pH/redox-sensitive IR825-loaded fluorescent carbon dot (FNP[IR825]) for controlled photothermal (PTT) effect. FNP[IR825] can be successfully entered tumor site, that benzoic imine bonds broke down under acidic cancerous conditions and disulfide bonds are then reduced in glutathione (GSH), subsequently released therapeutic IR825. After the IR825 is released, the NIR laser are utilized for PTT effect that can efficiently perform cancer treatment through photothermal conversion. The system has shown its potential to be used for monitoring in selectively cancer cell fluorescent on/off mediated diagnosis and therapy.
김슬기,Akhmad Irhas Robby,이병찬,이기백,박성영 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.99 No.-
Enhancing therapeutic efficacy of drugs from reactive oxygen species (ROS) and glutathione (GSH)-responsive matrix and minimizing toxic effects on normal cells remains a challenge in programmableanticancer drug delivery. Herein, ROS- and GSH-responsive paclitaxel (PTX)-loaded polymer dot (PD)with mitochondria-targeting capability was designed by constructing diselenide linkage andtriphenylphosphonium (TPP) for tunable PTX release andfluorescence for cancer theranostics. PDTPPnanocarrier could improve the PTX stability after loading (PD-TPP(PTX)), and the cleavage ofdiselenide bond in the presence of H2O2 and GSH triggered the controllable PTX release, providing higherfluorescence intensity. As the levels of H2O2 and GSH are higher in cancer cells compared to normal cells,PTX was selectively released from PD-TPP in cancer cells, reducing cell viability ( 25%) and causingenhanced apoptosis of cancer cells compared to normal cells. The PD-TPP(PTX) selectivity was alsoreflected by distinctfluorescence intensity in HeLa and PC-3 cells (cancer) compared to CHO-K1 cells(normal). Furthermore, conjugated TPP promoted the PD-TPP(PTX) accumulation in mitochondria due tospecific targeting of TPP towards mitochondria, allowing PTX release in mitochondria of cancer cells. Hence, this approach could be a potential strategy to enhance therapeutic efficacy of cancer drugs andminimize the side effects on normal cells.
Redox-sensitive FRET-Based Polymer Dot with BODIPY for Fluorescence Imaging in Cancer Cells
김슬기,박성영 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1
We developed a drug delivery system based on a redox-sensitive polymer dot (PD) for diagnosing cancer. BODIPY and Paclitaxel, loaded into PD are released via disulfide-thiol conversion in response to high GSH amount in cancer cells. Paclitaxel selectively released in the cancer cell environment, enabling killing that showed 100% release at 10 mM GSH. The quenching effect caused by BODIPY loading is interfered by GSH, subsequently release BODIPY; hence, the death of cancer cells can be visually monitored. Confocal images confirmed cellular uptake in MDCK, HeLa, and MDAMB cells for drug release and bio-imaging. This fluorescence on-off system allows the development of a matrix that can be used as a selective nanocarrier for killing cancer cells by showing a distinction between normal cells and cancer cells in response to GSH levels. This work was supported by Korea National University of Transportation in 2019.
Fluorescence on-off system silver hybrid-polymer dot for bacteria detection and killing
김슬기,조현훈,박성영,박남식,이기백 한국공업화학회 2020 한국공업화학회 연구논문 초록집 Vol.2020 No.-
We studied an effective nanomaterial for the detection of bacteria and the measurement of antimicrobial activity using fluorescent polymer dots and silver nanoparticles. We developed a PVP@Ag:PD system that takes highly fluorescent form using catechol chemistry to avoid fluorescent reduction of PD-Ag due to FRET quenching. Positively charged PVP@Ag:PD promotes and aggregates with the bacteria surface which mostly negative charge, influencing the fluorescence of the system due to intermolecular interaction. Even at low concentrations, PVP@Ag:PD showed prominent and sensitive bacterial killing efficiency. Using this feature, we developed a system for detecting and killing bacteria that combines biosensors with antimicrobial activity. Thus, this system provides a possibility for future bacteria sensing and extermination. This was supported by Korea National University of Transportation in 2020.