Propionylthiocholine-Dithiobis 방법에 의한 사람과 몇몇 동물 혈청내의 Cholinesterase 활성도 측정

김순태,김성훈,도재철,이영호,모기철,허린수,Kim, Soon-tae,Kim, Sung-hoon,Do, Jae-cheul,Lee, Young-ho,Mo, Ki-cheul,Huh, Rhin-sou 대한수의학회 1987 大韓獸醫學會誌 Vol.27 No.1

Determination of cholinesterase activity is a routine practice in many laboratories to detect the influence of cholinesterase inhibiting drugs such as organophosphate and carbamate insecticides. Among many different methods to determine the cholinesterase activity, the present method was the most recent, simple and accurate one for routine test in clinics. The results obtained in sera of human and the different species of animals by means of the present method were as follows: $5.76{\pm}1.12U/ml$ in human, $3.37{\pm}0.83U/ml$ in german shepherd, $0.61{\pm}0.18U/ml$ in rat, $14.91{\pm}3.10U/ml$ in mouse, $1.55{\pm}0.51U/ml$ in chicken, $0.28{\pm}0.11U/ml$ in slaughtered cattle and $0.50{\pm}0.10U/ml$ in slaughtered pig.

BB 랫드 및 streptozotocin이 투여된 랫드에서 vitamin E와 insulin 병합 투여 영향 I. 지질과 단백질의 산화성 손상 및 혈청내 효소 활성도에 미치는 영향

김순태,허린수,Kim, Soon-tae,Huh, Rhin-sou 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.4

The present study, to evaluate the effect of vitamin E on the oxidative stress in STZ-treated rat and BB rat, was investigated the biochemical enzyme activity in the serum, and malondialdehyde and carbonyl group in the RBC membrane, liver and microsomal fraction after vitamin E and/ or insulin treatment. Results obtained through the experiments were summarized as follows; 1. Effect of vitamin E and/or insulin treatment in STZ-treated rat 1) Lipid peroxidation level in RBC membrane, liver and microsomal fraction was significantly decreased in vi. tamin E and/or insulin treatment group, and especially more significantly decreased in vitamin E with insulin treated group. 2) Protein oxidation level in RBC membrane, liver and microsomal fraction was significantly decreased in vitamin E and/or insulin treatment group. And it was especially more significantly decreased in RBC membrane and liver of vitamin E with insulin treated group. 3) In the enzyme activity in the serum, the activity of AST and ALT was not altered in all experimental group. The increased ALP activity in STZ-treated group was significantly decreased in insulin treated group and vitamin E with insulin treated group. 4) Decreased level of albumin and creatinine after STZ treatment was significantly increased in vitamin E and/or insulin treated group. 5) Level of glucose, cholesterol and triacylglycerol in serum: Glucose level was not significantly different in vitamin E treated group compared to STZ control group. But it was significantly different in the insulin treated group and vitamin E with insulin treated group compared to STZ control group. The cholesterol content in the serum was significantly increased in STZ control group compared to normal control group. And except low dose vitamin E treatment group, it was significantly decreased in vitamin E and/or insulin treated group compared to STZ control group. The triacylglycerol content in the serum was significantly decreased in STZ control group and increased in high dose vitamin E treated group and vitamin E with insulin treated group. But it was not significantly different in low dose vitamin E treated group and insulin treated group compared to STZ control group. 2. Effect of vitamin E and/or insulin treatment in BB rat 1) Lipid peroxidation level in liver was decreased by vitamin E with insulin treatment compared to insulin treatment. But it was not different in microsomal fractions. 2) Protein oxidation level in liver and microsomal fraction was decreased by vitamin E with insulin treatment compared to insulin treatment only in microsomal fractions. These results suggest that the combination treatment of vitamin E and insulin could prevent the oxidative change of lipid and protein of the RBC membrane, liver and microsomal fraction in STZ-treated rats and BB rats.

BB 랫드 및 streptozotocin이 투여된 랫드에서 vitamin E와 insulin 병합 투여 영향 II. 인지질의 지방산 조성에 미치는 영향

김순태,허린수,Kim, Soon-tae,Huh, Rhin-sou 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.4

The present study was investigated fatty acid composition of the phospholipid in the RBC membrane, liver and microsomal fraction after vitamin E and/or insulin treatment to evaluate the effect of vitamin E on the oxidative stress in STZ-treated rat and BB rat. Results obtained through the experiments were summarized as follows; 1. Effect of vitamin E and/or insulin treatment in STZ-treated rat 1) In the insulin treated group and the combination treated groups of vitamin E with insulin, body weights were increased compared to STZ-treated rat(STZ control group). Especially it was more significantly increased in the combination treated group of high dose vitamin E with insulin. 2) The composition of fatty acids of the phospholipid in RBC membrane, liver and microsomal fractions was shown a decreased C16:1, C18:1, C20:4 and an increased C16:0, C18:0, C18:2 in STZ control group compared to normal control group. In RBC membrane, liver and microsomal fractions after vitamin E with insulin treatment in STZ-treated rat, effect on the composition of fatty acids of the phospholipid was shown the result of a decreased C16:0, C18:0, C18:2 and an increased C16:1, C18:1, C20:4. 3) Hemolysis rate of the RBC to $H_2O_2$ was increased in the STZ control group and it was decreased below the hemolysis level of normal control group by vitamin E treatment. 2. Effect of vitamin E and/or insulin treatment in BB rat 4) Only in microsomal fraction, fatty acid composition was different between insulin treatment group and vitamin E with insulin treatment group. It was increased C16:0 and C18:1, and decreased C18:0 and C18:2 in vitamin E with insulin treatment group: But C20:4 was not different in two groups. These results suggest that the combination treatment of vitamin E and insulin could prevent the oxidative change of fatty acids in P-lipid of the RBC membrane, liver and microsomal fraction in STZ-treated rats and BB rats.

소상공인 정책자금의 성과분석 연구

김순태(Kim, Soon-Tae),민경명(Min, Kyung-Myung),오상영(Oh, Sang-Young) 한국산학기술학회 2012 한국산학기술학회논문지 Vol.13 No.4

정부의 소상공인정책자금 지원에 대한 효과 분석을 통하여 지원 자금 규모에 따라 소상공인의 매출 증가 변 화, 향후 성장성을 분석하였다. 정부자금의 수혜기업과 비수혜기업을 구분하여 상호 비교하여 분석하거나 수혜기업 중심으로 지원 자금의 성과를 분석하여 결과를 제시함으로서 정부의 자금 지원정책에 시사점을 제공하였다. This study has focused on analyzing the changes of increasing sales and potential growth by different scales of support with funds through effect analysis on governmental policy loan. It provided significant findings for the further governmental policy loan since it conducted the performance analysis of different business respectively, which included business with the governmental policy loan and ones without any loan.

사염화탄소(四鹽化炭素)를 투여(投輿)한 MICE 혈청(血淸)내 효소(酵素) 활성(活性) 및 생화학적(生化學的) 조성(造成)의 변화(變化)

김순태 ( Soon Tae Kim ),손재권 ( Jae Kweon Son ),김상윤 ( Sang Yun Kim ) 한국가축위생학회 1993 韓國家畜衛生學會誌 Vol.16 No.2

Carbon tetrachloride (CCl4) has been used to treat a variety of parasitic infection in both large and small animals, including Fasciola hepatica. Recently, we can easily contest with carbon tetrachloride in air, food, water, rain and industry area etc. and it is using in order to induce liver injury in laboratory. In this study, we investigated activitis of LDH, ALP, AST and ALT and amount of cholesterol, triacylglycerol, glucose and BUN in mice serum exposed to CCl4. The mice divided into a Naive control(A), corn oil control(B) and experimental group(C, D). Naive control group(A) was given feed and water only. Corn oil control group(B) was given corn oil lml/100g of body weight(B.W). Experimental group(C) was given carbon tetrachloride 1ml/l00g B.W.(CCl4:corn oil=1:20(V/V)). Experimental group(D) was given carbon teterachloride 1ml/100g B.W.(CC14:corn oil=1:10(V/V)). The results obtained were summarized as follows: 1) The body weight was declined after the 10th day in mice exposed to carbon tetrachloride. 2) The total protein level in serum was significantly in mice exposed to carbon tetrachloride(P≤0.05). The albumin and A/G ratio was decresed significantly in mice exposed to carbon tetrachloride (P≤0.01). 3) All of the activity of LDH, AST, ALT and ALP in mice serum exposed to carbon tetrachloride: incresed significantly activity of LDH(P≤0.05), incresed significantly activity of AST(P≤0.05, P≤0.01), incresed significantly activity of ALT and ALP(P≤0.05, P≤0.01). 4) The amount of cholesterol and triacylglycerol, lipid metabolite products in serum was incresed in case of cholesterol but did not change in case of triacylglycerol.

도시개발사업에서 체비지 담보신탁 수익권 확보방안 - 위탁자인 조합의 체비지 임의매각 사례를 중심으로 -

김순태 ( Kim Soon-tae ),한재경 ( Han Jae-kyung ) 건국대학교 법학연구소 2021 일감부동산법학 Vol.- No.22

도시개발법에 따라 추진되는 도시개발사업에서 환지 예정지 상태의 체비지를 도시개발조합이 신탁사에게 담보신탁을 통해 소유권이전을 할 경우에 당해 체비지에 대하여 담보신탁현황을 체비지 관리대장에 등재하더라도 환지처분공고 다음날 소유권보존등기 후 신탁사 모르게 제3자에게 처분하게 되면 담보신탁의 수익권자는 재산권에 피해를 입을 수밖에 없다. 따라서 환지 예정지 상태에서 체비지를 도시개발조합으로부터 매입한 매수인은 체비지 관리대장인 체비지원부의 기재를 통해 등기원인이 생긴 것임을 증명함으로써 소유권 이전등기를 마친 때에 소유권을 취득하도록 도시개발법 제42조 제5항 단서를 개정하고, 관련 등기 행정규칙에 체비지 관리대장을 도시개발조합의 소유권보존등기 촉탁·신청시 첨부서류로 의무화함으로써 체비지 처분대금이 도시개발사업과 무관하게 도시개발조합에 의해 유용되지 않도록 하여야 할 것이다. In the case of an urban development project promoted in accordance with the Urban Development Act, if the urban development association transfers ownership of the ‘land allotted by the authorities in recompense for development outlay’(hereinafter referred to as ‘substitute land’) in the state of the replotting site through a collateral trust to a trust company, the ownership of the refurbished land will be preserved the day after the announcement of the refurbishment disposition, even if the status of the collateral trust status is registered in the deserved land management ledger. After registration, the beneficiary of the mortgage trust is bound to suffer damage to property rights if it is disposed of to a third party without the trust company’s approval. Therefore, the proviso to Article 42 (5) of the Urban Development Act is amended to ensure that the buyer who purchased the substitute land from the urban development association in the state of the reserved land for replotting will acquire ownership upon completion of the registration of the transfer of ownership by proving that the cause of registration has occurred through listing on the substitute land management ledger. In addition, it is necessary to ensure that the amount of disposal of the substitute land is not misappropriated by the urban development association by making it mandatory for the urban development association to request and apply for ownership preservation registration according to the relevant registration administrative rules.

MPEG-4 형상오류은닉을 위한 하드웨어 아키텍쳐

김순태(Soon Tae Kim),조원경(Won Kyung Cho) 대한전자공학회 2001 대한전자공학회 학술대회 Vol.2001 No.11

돼지 및 닭에서 분리된 균에 대한 봉독의 최소억제농도(MIC)

김순태 ( Soon Tae Kim ),황지영 ( Ji Young Hwang ),성명숙 ( Myoung Suk Sung ),제소양 ( So Yang Je ),배동록 ( Dong Rok Bae ),한상미 ( Sang Mi Han ),이성해 ( Sung Hae Lee ) 한국가축위생학회 2006 韓國家畜衛生學會誌 Vol.29 No.1

Bee venom is synthesized in the venom gland`s of worker and queen bees and stored in their venom sacs. Bee venom is a rich source of enzymes, peptides and biogenic amines. there are at least 18 active components in the venom which have some pharmaceutical properties. This study was performed to evaluate minimum inhibitory concentration(MIC) of bee venom against bacteria isolated from pigs and chickens with disease. In case of reference strains, the MIC(㎍/㎖) of Staphylococcus aureus ATCC 6538, Streptococcus mutans ATCC 25175, and Salmonella typhimurium ATCC 6538 were 64, 64 and 32, respectively. In case of bacteria isolated from pig and chicken, the MIC of Staphylococcus aureus, Staphylococcus hyicus and Staphylococcus chromogenes were 8, 128 and 128, and that of 11 strains of Escherichia coli were 8 to >512 and that of 8 strains of Salmonella spp were >512. Antibacterial resistance test of 22 strains isolated from pig and chicken and 3 reference strains were performed by agar gel diffusion method, using 17 antibacterial drugs including penicillin, cefazolin, tetracycline and quinolone group. The multiple drug resistant patterns were found in most strains isolated from pig and chicken.

도축 타조에서 닭 및 돼지 질병에 대한 혈청학적 조사

김순태 ( Soon Tae Kim ),박인화 ( In Hwa Park ),김영환 ( Young Hoan Kim ),조광현 ( Kwang Hyun Cho ),오규실 ( Kyu Shil Oh ),손재권 ( Jae Kweon Son ),정종식 ( Jong Sik Jyeong ) 한국가축위생학회 2004 韓國家畜衛生學會誌 Vol.27 No.3

As all other intensively farmed domestic species, most mortality in ostriches is closely to rearing conditions. While ostriches is also highly sensitive to stress, species-specific infectious disease play only a minor role. But investigation of ostrich`s disease is not performed almost in Korea. The study was performed to investigate the titers of antibody for Newcastle disease(ND), Infectious bronchitis(IB), Egg drop syndrome ``76(EDS), Avian influenza(AI), salmonellosis, Mycoplasma gallisepticum infection(MG), Mycoplasma synoviae infection(MS), Infectious bursal disease(IBD), Brucellosis, Toxoplasmosis, Japanese encephalitis(JE), Porcine parvovirus infection, Encephalomyocarditis and Porcine reproductive respiratory syndrome (PRRS). The results obtained in the 62 ostrich sera slaughtered in Gyeongbuk province were summarized as follows: The average of antibody positive rates to ND, IB, EDS, AI(H9N1), JE, Porcine parvovirus infection and Encephalomyocarditis by HI test were 75.8%, 100%, 0%, 0%, 51.6%, 50% and 56.5% respectively. The antibody positive rates to salmonellosis, MG, MS by plate agglutination test were 12.9%, 25.8%, and 0% respectively. Antibodies to disease agent such as IBD and AI by agar gel precipitation(AGP) test, Brucellosis by tube agglutination, toxoplasmosis by latex agglutination test and PRRS by IFA were all negative.

Sodium chloride와 sodium carbonate를 첨가한 yellow alkaline noodle sheet의 물리적 특성

김순태(Soon Tae Kim),장학길(Hak-Gil Chang),박영서(Young-Seo Park) 한국식품과학회 2007 한국식품과학회지 Vol.39 No.1

밀가루에 소금을 0, 2 및 4%를 첨가하였을 경우, Mixograph의 peak time은 밀가루의 종류에 관계없이 소금 첨가량이 증가함에 따라서 증가함을 알 수 있었으나 박력분의 경우에는 유의적인 차이를 나타내지는 않았다. Peak height와 width at peak도 소금 첨가량이 증가함에 따라 증가하는 경향을 나타내었으나 강력분과 중력분에서는 유의적인 차이를 나타내지 않았다. Width at 8 min의 경우에는 모든 밀가루에서 소금 첨가량이 증가함에 따라 증가하는 경향을 나타내었다. Sodium carbonate를 0, 0.5, 1.0 및 1.5의 농도로 밀가루에 첨가하여 Mixograph 특성을 관찰한 결과 밀가루의 종류에 관계없이 0.5% 첨가 시 peak time이 거의 2배정도 증가하였다가 그 보다 높은 농도에서는 다시 감소된 반면, peak height는 sodium carbonate 첨가량이 증가함에 따라서 감소하는 경향을 보였다. Mixograph peak time과 단백질 함량 및 침전가와는 유의적인 상관이 없었으나, peak height, width at peak 및 width at 8min과는 모두 정의 상관관계를 보여 주었다. 반죽의 초기호화온도, 최고점도, 최소점도, 최종점도는 밀가루의 종류에 관계없이 소금의 첨가량이 증가함에 따라 증가하는 경향을 나타내었으며, 최고점도, 최소점도 및 최종점도는 모든 밀가루에서 sodium carbonate 첨가량이 증가함에 따라서 유의적으로 증가하는 경향을 보였다. 초기호화온도와 보수율 및 알칼리수 흡수율과는 정의 상관이 있었으나, 보수율과 최고점도, 최소점도 및 최종점도와는 모두 부의 상관관계를 보여 주었다. The peak time, peak height, width at peak, and the width at 8 min of Mixograph increased as the amount of sodium chloride in yellow alkaline noodle sheet was increased. The peak time of the Mixograph duration increased almost twice when 0.5% sodium carbonate was added to yellow alkaline noodle sheet, but decreased when the amount of sodium carbonate was above 0.5%. The peak height decreased as the amount of sodium carbonate increased. Protein content and sedimentation values showed positive correlations with the Mixograph peak height, width at peak, and width at 8min. The pasting temperature, peak viscosity, minimum viscosity, and final viscosity increased as the amount of sodium chloride in yellow alkaline noodle sheets was increased for all wheat flours. The peak viscosity, minimum viscosity, and final viscosity also increased as the amount of sodium carbonate increased. The pasting temperature showed a positive correlation with the water retention capacity and the alkaline water retention capacity.