Stigmatella aurantiaca의 발생에 대한 연구(I) -fruiting body 형성에 미치는 몇가지 양이온과 pheromone 및 GMP의 영향-

김수옥,김영민,Kim, Soo-Ok,Kim, Young-Min 한국미생물학회 1984 미생물학회지 Vol.22 No.1

Stigimatella aurantiaca는 calcium, barium, 또는 lithium이온이 포함된 배지에서는 빛을 비추어 주어도 stalk를 형성하지 않고 포자낭으로만 분화하였다. Calcium이온과 GMP가 함께 첨가된 배지(GMP 배지)에서는 빛의 유무에 관계없이 stalk와 포자낭을 지니는 fruiting body가 형성되었다. Calcium 이온과 pheromone 포함된 배지(pheromone배지)에서는 빛이 있는 경우에는 대부분의 세균들이 stalk로만 분화되었으나, 빛이 없는 경우에는 대부분 포자낭으로만 분화하였다. 세균집합체는 calcium과 potassium 및 sodium이 함꼐 첨가된 배지(CPS배지)에서보다 calcium 이온만 첨가된 배지 (calcium 배지)에서 더 많이 형성되었다. GMP 배지나 pheromone 배지에서는 calcium 배지에서보다 적은 수의 세균집합체가 형성되었고, 빛이 없는 경우에는 이 집합체의 형성이 소요된 시간이 단축되었다. 빛은 상기한 네가지 배지에서 공히 짧은 시간에 더 많은 세균집합체가 형성되도록 하였다. Cells of Stigmatella aurantiace developed in the light on the medium containing calcium, barium, or lithium ion formed fruiting bodies without stalk. Fruiting body with stalk was formed on the medium containing calcium ion and GMP (GMP-medium) even under the dark condition. On the medium containing calcium and pheromone (pheromone-medium), most cells were developed only into the stalk in the light and into the sporangium in the dark. The number of aggregate formed on the medium containing calcium ion (Ca-medium) was more than that formed on the medium containing calcium, potassium, and sodium ions (CPS-medium). The number of aggregate formed on the GMP or pheromone-medium was less than that formed on the Ca-medium. Both pheromone and GMP reduced the time required for aggregate formation when cells were developed in the dark. Light stimulated cells to form more aggregates in short time when it was introduced into the Ca-, CPS-, GMP-, or pheromone-medium.

폐결핵 진단 시 중합효소연쇄반응검사 반복 시행의 의의

김수옥 ( Soo Ok Kim ),김윤희 ( Yoon Hee Kim ),지수영 ( Su Young Chi ),반희정 ( Hee Jung Ban ),오인재 ( In Jae Oh ),권용수 ( Yong Soo Kwon ),김규식 ( Kyu Sik Kim ),김유일 ( Yu Il Kim ),임성철 ( Sung Chul Lim ),김영철 ( Young Chul K 대한결핵 및 호흡기학회 2010 Tuberculosis and Respiratory Diseases Vol.68 No.6

Background: The polymerase chain reaction (PCR) test is important for the confirmatory diagnosis of tuberculosis (TB) caused by Mycobacterium tuberculosis. The aim of this study was to analyze the yield of repeated PCR testing in patients with confirmed pulmonary TB. Methods: The medical records of 130 patients, who had more than two consecutive PCR tests and a M. tuberculosis- positive sputum culture from August, 2006 to December, 2007, were retrospectively reviewed for the purposes of this study. A positive TB-PCR test was defined as at least one positive test result. Results: The cumulative positive PCR test rate was 80% (104/130), with gradually increasing rates of positive findings upon the first, second and third TB-PCR tests with 52.3%, 68.5% and 75.4%, respectively. However, further testing did not increase the positive rate further. Conclusion: Repeated PCR testing at least three times for M. tuberculosis is helpful for diagnosis of pulmonary TB.

임상간호사의 감정노동, 긍정자원 및 직무소진에 대한 연구

김수옥(Kim, Soo-Ok),왕미숙(Wang, Mee-Suk) 한국산학기술학회 2015 한국산학기술학회논문지 Vol.16 No.2

본 연구는 임상간호사를 대상으로 감정노동, 긍정자원 및 직무소진의 수준을 확인하고 이들의 관계를 파악하기 위하 여 시도되었다. 연구 대상자는 서울소재 일 공립병원에 근무하는 간호사 217명이며, 자료수집은 2014년 10월 6일 부터 10 월 30일 까지 실시하였다. 수집된 자료는 PASW statistics 18.0 프로그램을 이용하여 전산통계 처리하였으며, 빈도, 백분율, 평균, 표준편차, t-test, ANOVA 및 사후검증, Pearson??s correlation으로 분석하였다. 감정노동은 5점 중 평균 3.5점, 긍정자 원은 5점 중 평균 3.4점, 그리고 직무소진은 5점 중 평균 2.8점으로 나타났다. 감정노동과 직무소진간은 유의한 양의 상관관계 (r=.342, p<.001)를 나타냈고, 긍정자원과 직무소진간은 유의한 음의 상관관계(r=-.606, p<.001)를 나타냈다. 본 연구 결과는 임상간호사의 직무소진을 예방하기 위하여 긍정자원을 증가시키고 감정노동을 감소시키기 위한 중재 프로그램 개발이 필요함을 보여주었다. This study was to investigate the relationships of emotional labor, positive resources and job burnout. The participants were 217clinical nurses are working on a hospital in Seoul. The mean score of emotional labor, positive resources, and job burnout were 3.5, 3.4, 2.8 respectively. Job burnout showed positive correlation with emotional labor(r=.342, p<.001) and showed negative correlation with positive resources(r=-.606, p<.001). The finding of this study indicate a need to develop program to increase positive resources and control the emotional labor in order to prevent job burnout.

여자 중학생의 음주경험과 금주의도 및 관련요인

김수옥 ( Soo Ok Kim ) 대한간호학회정신간호학회 2008 정신간호학회지 Vol.17 No.1

정신질환자의 흡연지식, 흡연태도 및 금연의도에 관한 연구

김수옥 ( Soo Ok Kim ) 한국정신간호학회 2007 정신간호학회지 Vol.16 No.1

금연클리닉 등록자의 금연행위 예측요인에 관한 연구

김수옥 ( Soo Ok Kim ) 대한간호학회정신간호학회 2009 정신간호학회지 Vol.18 No.3

간호대학생의 노인에 대한 지식, 태도 및 행동에 대한 연구

김수옥(Kim, Soo Ok),오효순(Oh, Hyo Soon),왕미숙(Wang, Mee Suk) 한국간호교육학회 2016 한국간호교육학회지 Vol.22 No.2

Purpose: The purpose of this study is to determine the relationship between nursing students’ level of knowledge of and attitude toward the elderly and their behavior towards the elderly. Methods: For this study, 223 nursing students participated. The data was analyzed with PASW statistics program version 18.0 using descriptive statistics, a t-test, one-way ANOVA, Pearsons Correlation coefficient and multiple regression analysis. Results: The findings of the study displayed a score of 14.5 out of 25 for the level of knowledge of the elderly, a score of 3.9 out of 7 on the attitude toward the elderly, and 1.3 points on a 4-point scale on behavior towards the elderly. There was a positive correlation (r=.410, p<.001) between attitude and behavior towards the elderly, thus confirming that attitude towards the elderly influences behavior towards them. Conclusion: The most influential variable on the behavior towards the elderly emerged to be attitude. Thus, although knowledge and attitude cannot be neglected in manifesting positive behaviors in nursing students when they care for the elderly, knowledge training and elderly nursing practical sessions need to take place in order to strengthen attitude, which displayed a positive correlation with behavior.

지역사회 거주노인의 수면장애 발생빈도와 위험요인

김수옥(Kim Soo-Ok),이경희(Lee Gyung-Hee) 계명대학교 간호과학연구소 2005 계명간호과학 Vol.9 No.1

  Purpose: The purpose of this study was to identify the prevalence and risk factors of sleep disturbance among the community resident elderly. Method: 108 elderly were included in this study and data were analyzed using descriptive statistics including x²-test, t-test and stepwise discriminant analysis. Result: The prevalence of sleep disturbance was 59.2%. The significant variables which explain the occurrence of sleep disorder were depression, health state and age. Depression was the most important risk factor, the hit ratio of discriminant function was 63.9%. Conclusion: From this study, we now know that it is very important to develop intervene stratege for the depression to promotion sleep of the elderly.

생약복합제제의 약효 연구 (제18보) : 팔미환이 신성고혈압에 미치는 영향

노영수(Young Soo Rho),홍남두(Nam Doo Hong),김수옥(Soo Ok Kim),김남재(Nam Jae Kim) 한국생약학회 1984 생약학회지 Vol.15 No.4

To investigate the recorded efficacy in Oriental medical references and effects in clinics, experimental studies were undertaken to estimate Palmiwhan to the diuretic effect in normal rats, rabbits and rats with HgCl₂-induced acute renal failure and hypotensive effects on the nephrogenous hypertension in rabbits with HgCl₂-induced acute renal failure. The results of this studiy were summerized as follows; Increase in urinary volume, urinary Na^+ and Cl^- excretions were significantly recognized in normal rats and rabbits. Increase in urinary volume, urinary Na^+ and Cl^- excretions were significantly shown in rats with 2㎎/㎏ HgCl₂-induced acute renal failure. Although plasma electrolyte and creatinine levels were little affected, plasma urea nitrogen levels were significantly elevated in normal rats and 2㎎/㎏ HgCl₂-induced acute renal failure of rats. Although the blood pressure was not affected in normal rabbits and cats, hypotensive actions due to the diuretic effect were recognized in rabbits HgCl₂-induced acute renal failure.

Screening of Effective Medium Composition for the Cultivation of Lactobacillus plantarum and Lactobacillus reuteri Using Statistical Methods

Dong Woon Kim(김동운),Sang Buem Cho(조상범),Young Hwa Kim(김영화),Sung Daw Lee(이성대),Hyun Jung Jung(정현정),Sang Ho Kim(김상호),Kyu Ho Cho(조규호),Soo Jin Sa(사수진),In Cheul Kim(김인철),Mi-Young Won(원미영),Su-Ok, Kim(김수옥),Soo- 한국생명과학회 2012 생명과학회지 Vol.22 No.5

본 연구는 가축용 생균제로서 이용성이 우수한 Lactobacillus plantarum과 Lactobacillus reuteri의 대량생산을 위한 경제적 최적 배지조성을 도출하기 위하여 수행되었다. 두 균주의 배양을 위한 배지성분 요인들은 유산균 배지로 알려진 MRS broth를 기준으로 하여, sucrose, glucose, molasses, yeast extract, corn steep liquor, soy peptone, dipotassium phosphate, manganese chloride, magnesium chloride, tween 80, sodium chloride, sodium acetate, ammonium citrate, sodium sulphate 및 ferrous sulphate 등 총 15가지를 선택하였다. 각 배지성분들이 각 균주들의 세포성장에 미치는 요인분석은 20개의 실험으로 구성된 Plackett-Burman design 방법을 이용하여 분석하였다. 그 결과 L. plantarum의 성장에 있어서는 sucrose, glucose, molasses, yeast extract, corn steep liquor, soy peptone, sodium acetate, ammonium citrate 등이 긍정적인 효과(positive effect)를 나타내었다. L. reuteri의 경우, yeast extract, soy peptone, K2PHO4, tween 80에서 수준간의 격차가 큰 긍정적인 효과가 나타났다. 두 가지 균주들에서 얻어진 표준화된 통합적 효과의 주 효과를 분석한 결과, sucrose, yeast extract, soy peptone에서 긍정적인 효과가 나타났다. 최종적으로 L. plantarum과 L. reuteri 두 균주에 공통적으로 적용할 수 있는 유효 배지 성분들로 sucrose (20.0 g/l), glucose (5.0 g/l), soy peptone (11.0 g/l), yeast extract (5.0 g/l), K2HPO4 (0.2 g/l), CH3COONa (2 g/l) 및 MgCl2 (0.02 g/l)를 선택하였다. This study was conducted to develop an economical optimum medium composition for the mass production of Lactobacillus plantarum and Lactobacillus reuteri, livestock probiotics. Medium ingredient factors were selected on the basis of MRS broth composition, and the 15 ingredient variables were as follows: sucrose, glucose, molasses, yeast extract, corn steep liquor, soy peptone, dipotassium phosphate, manganese chloride, magnesium chloride, tween 80, sodium chloride, sodium acetate, ammonium citrate, sodium sulphate, and ferrous sulphate. The Plackett Burman design, consisting of 20 runs, was employed for the analysis of ingredient effects on cell growth of L. plantarum and L. reuteri. As a result, sucrose, glucose, molasses, yeast extract, corn steep liquor, soy peptone, sodium acetate, and ammonium citrate positively influenced the growth of L. plantarum. Additionally, yeast extract, soy peptone, K2PHO4, and tween 80 positively influenced the growth of L. reuteri. Positive effects were found from sucrose, yeast extract, and soy peptone in the integrated analysis of the effects of both L. plantarum and L. reuteri. Finally, effective medium components for both strains were found as follows: sucrose (20.0 g/l), glucose (5.0 g/l), soy peptone (11.0 g/l), yeast extract (5.0 g/l), K2HPO4 (0.2 g/l), CH3COONa (2 g/l), and MgCl2 (0.02 g/l).