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김세현,정남철,나천수,김삼식 ( Sea Hyun Kim,Nam Chul Jung,Chun Soo Na,Sam Sik Kim ) 한국산림과학회 1998 한국산림과학회지 Vol.87 No.2
The stimulated secretion of golden varnish sap by the infection of microorganisms on the bark of Dendropanax morbifera Lev. was observed in the natural habitats. Four candidate strains of such microorganisms(i.e., Sb1, Sb2, Sb3 and Sb4) were isolated from the bark of D. morbifera which was infected in natural. Of them, Sb3 and Sb4 strains showed stimulated secretion of golden vernish sap with the greatest stimulation by Sb4 strains. The yield of golden varnish sap from the bark of D. morbifera was boosted about 3.4--7.7 times by the inoculation of Sb4 strain in compassion with that collected from lacquer tree(Rhus verniciflua Stokes) by the traditional method. The mycelium color of Sb4 strain was grayish white. The optimum growth temperatures and pH were ranged from 30 to 35℃ and from pH 6.5 to 7.0.
돌배나무류 과실의 에탄올 추출물의 페놀성 화합물 함량 분석
김세현 ( Sea Hyun Kim ),심상희 ( Sang Hee Shim ),김혜수 ( Hyeu Soo Kim ),송정호 ( Jeong Ho Song ),김문섭 ( Moon Sup Kim ) 한국임학회 2014 산림과학 공동학술대회 논문집 Vol.2014 No.-
돌배나무류( Pyrus spp.)는 장미과(Rosaceae)에 속하는 낙엽활엽수로써, 열매는 식용 뿐만 아니라 해열, 건위, 지갈, 이뇨, 항당뇨 등의 효능이 있어 민간약으로 널리 사용되어왔다. 돌배나무류는 돌배나무( P. pyrifolia (Burm. f.) Nakai), 산돌배( P. ussuriensisMaxim.), 백운배나무( P. ussuriensisvar.hakunensis (Nakai) T. B. Lee), 정선배나무( P. pyrifolia (Burm. fil.)(Jeongseon)), 청실배나무( P. ussuriensis Max. var. ovoidea Rehder) 등 종류가 다양함에도 불구하고 수종별 생리활성 및 성분 연구는 미비하다. 본 연구에서는 돌배나무류의 다양한 열매를 이용하여 기능성 소재를 발굴하기 위하여 생리활성 성분을 비교·분석하였다. 실험에 사용된 HPLC는 Agilent사의 1200 series를 사용하였고, column은 Luna C18(10.00×250mm, 5μm, Phenomenex)을 사용하였으며, 검출기는 UV 280nm, 이동상으로는 0.1% TFA를 첨가한 acetonitrile과 0.1% TFA를 첨가한 물을 사용하여 gradient profile로 하였고, 유속은 2.0ml/min으로 하였다. 돌배나무류 70% 에탄올 추출물을 profiling한 결과, phenol성화합물 중 arbutin과 chlorogenic acid가 주로 함유되어 있었다. 특히, chlorogenic acid의 함량(2.48~14.36mg/g)이 arbutin(1.02~7.83mg/g)보다 더 높은 것으로 확인되었으며, chlorogenic acid와 arbutin의 평균함량은 각각 돌배나무가 6.8, 2.8mg/g, 산돌배가 7.6, 3.5mg/g, 정선배나무가 3.3, 1.4mg/g, 백운배나무가 10.5, 2.6mg/g, 청실배나무가 3.8, 1.0mg/g로 수종별로 상이하게 나타났다. The Korean wild pears have been considered not only fruit but an herbal medicine in East Asia. The fruit of this plant, known as a pear, is common and highly consumed in Korea. Although varieties of Pyrus were various, the study about bioactive substance and bioactivity of Pyrus varieties was incomplete. The study aims to assess bioactive substance of 70% ethanol extracts of 5 wild pear species in Korea and its potential as a medicinal ingredient of functional food and medicine. The used HPLC system was a Agilent 1200 series(Agilent, USA) equipped with a Luna C18 Column(10.00×250mm, 5μm, Phenomenex), coupled with a UV detector(280nm). The mobile phase consisted of 0.1% TFA in actonitrile, water at a flow rate of 2.0ml/min. The result of profiling HPLC chramatogram from 70% ethanol P. spp. extracts showed that the major phenolic compound was found as chlorogenic acid, arbutin. In the 70% ethanol extracts of P. spp., chlorogenic acid(2.48~14.36mg/g) was more than arbutin(1.02~ 7.83mg/g) and the chlorogenic acid and arbutin content were significant differences among the P. pyrifolia (Burm. f.) Nakai(6.8, 2.8mg/g), P. ussuriensis Maxim.(7.6, 3.5mg/g), P. pyrifolia (Burm. fil.)(Jeongseon)(3.3, 1.4mg/g), P. ussuriensis var. hakunensis (Nakai) T. B. Lee)(10.5, 2.6mg/g) and P. ussuriensisMax. var. ovoidea Rehder(3.8, 1.0mg/g).
김세현 ( Sea Hyun Kim ),정헌관 ( Hun Gwan Chung ),장용석 ( Yong Seok Jang ),박영기 ( Young Ki Park ),박형순 ( Hyung Soon Park ),김선창 ( Sun Chang Kim ) 한국산림과학회 2005 한국산림과학회지 Vol.94 No.1
Fruit characteristics, saccharinity, and antioxidalive activity of selected clones of Rubes coreanus, grown in Korea, well-known as edible and medical resources, frequently used and which has an excellent pharmacological activity are examined and analyzed. The results obtained from this study can be summarized as follows; Result of surveying characteristics for the fruit and saccharinity variation by collection time, there are some meaningful differences between examined clones and the collection time, exception of the fruit width. From the clones, SG 3 is the biggest in size, the highest in the saccharinity out of five clones, on the other hand, CJ 18 and SH 3 are the opposite. Meanwhile, comparing examined saccharinity factor with maximum temperature and precipitation out of daily climates at Suwon Meteorological Observatory at the same period, on the day the highest temperature is 29.5℃, saccharinity distinctively ascends, but on the day the precipitation is recorded 121.5 mm and next day. the saccharinity descends. Also, five-day saccharinity until the precipitation lower than 0.1 mm per day shows from 10,4 to 11.5 Brix higher than the average saccharinity of 9.7 Brix. Fifteen clones including CA 6 about fruit characteristics in terms of collection layer and saccharinity variation were examined, there is significantly difference among examined clones. but about the collection layer, only saccharinity factor has meaningful significance. For clones, MJ 11 is the biggest and excellent character of 15 clones but the saccharinity, 10.3 Brix lows a bit, MC 9 and HAE 5 are the worst fruit in character but saccharinity is 13.4 Brix and 10.6 Brix which is higher than the average saccharinity of 9.8 Brix. About saccharinity for the collection layer, the fruit average saccharinity, collected from the upper layer which is good at lightness and temperature condition, is 10.2 Brix which shows higher than the one of the fruit, 9.7 Bix and 9.5 Brix collected from the middle or the lower layer. The saccharinity of fruit collected from the middle or the lower layer is lower than the average saccharinity of 9.8 Brix out of 15 surveyed clones. Surveying antioxidative activity resulted from free radical scavenging activity about 30 clones of fruit in total, there is difference between surveyed clones. Especially, Tmmatured fruit extracts exhibited higher free radical scavenging activity of 94.5 to 97.5% which indicates the similarity to Ascorbic acid, unlike other clones, on the concentration of 250 to 1,000 ppm.
김세현 ( Sea Hyun Kim ),전동하 ( Dong Ha Jun ),장민정 ( Min Jung Jang ),이진태 ( Jin Tae Lee ),이창언 ( Chang Eon Lee ),한진규 ( Jing Yu Han ),김진철 ( Jin Chul Kim ),이도형 ( Do Hyung Lee ) 한국산림과학회 2010 한국산림과학회지 Vol.99 No.6
Hovenia dulcis var. koreana Nakai has been reported to liver function improvement effect as functional materials for food and medicine. On these facts, biological activity and safety test were conducted to evaluate biological activities of the fruit petiole and root extracts of H. dulcis as a potential cosmeceutical ingredient. Cosmeceutica activities of different extracts were examined by l.l-diphenyl-2-picrylhydrazyl (DPPH) radical generation, the ABTS+ cation decolorization, tyrosinase activity, collagenase activity and elastase activity compared with the properties of the commercial antioxidant butylated hydroxytoluene (BHT) and L-ascorbic acid(AA). The antioxidant activities HDFW, HDFE, HDRW and HDRE were 83.6%, 39.6%, 85.9% and 74.5% in DPPH assay, 99.5%, 13.7%, 96.4% and 88.6% in ABTS assay. Tyrosinase inhibitiory activities HDFW were 56.0% at 1,000 ppm. Measured the inhibition effect of the H. dulcis about collagenase and elastase where break the peptide bonds in collagen and enzyme from the class of proteases where exists in the dermis. The H. dulcis was inhibition the two kind enzymesm, collagenase activities being on a high scale inhibition, was same concentration. Uses the anti oxidation effect and a anti-wrinkle effect of this resultant H. dulcis and with the functional cosmetics use is thought with the fact that will be possible.
김세현(Sea Hyun Kim),신창호(Chang Ho Shin),정남철(Nam Chul Jung),나천수(Chun Soo Na),김영중(Young Joung Kim) 한국산림과학회 2000 한국산림과학회지 Vol.89 No.1
The ecological characteristics of 10 natural stands of Dendropanax morbifera(Haenam, Wando 1, Wando 2, Bogildo, Kemundo and Hongdo and Sendol, Suoak, Hannam, and Kidowon populations in Cheju island) were studied. In most of the Dendropanax morbifera natural stands, the following tree species appeared predominantly : Camellia japonica, Castanopsis cuspidate var. sieboldii, Castanopsis cuspidate var. thunbergii, Carpinus laxiflora, Quercus acute and Eurya japonica. Especially, Camellia japonica and Eurya japonica appeared in all the stands. Dendropanax morbifera occupied 17.2% of the upper story, 12.9% of the middle story, and 10.3% of the lower story, respectively. The distribution patterns by Morisita`s Index showed that Dendropanax morbifera was distributed randomly in all of the three stories, i.e. the upper, the middle and the lower stories. Dendropanax morbifera was positively associated with Camellia japonica, Eurya japonica, Carpinus laxiflora, Cinnamomum japonicum, Ligustrum japonicum, Callicarpa Japonica. Castanopsis cuspidate var. thunbergii, Castanopsis cuspidata var. sieboldii, Daphniphyllum macropodum, Acer palmatum and Euonymus oxyphyllus. This might suggest that the ecological niche is similar to each other. The cluster analysis using percent dissimilarity(PD) based on species composition divided into two groups. One group consisted of the populations located along the south-western coasts. The other group consisted of the populations of Cheju island. This result was agree with that of ordination analysis. From these results, We could find out that the species composition of the populations of Dendrapanax morbifera in Cheju island was quite different from those of other regions.