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Ultrastructural Localization of ZnT3 and Zinc Ions in the Mouse Choroid Plexus
김성주,김용국,손원재,김수진,정영길,유윤조,조승묵,Kim, Sung-Joo,Kim, Yong-Kuk,Sun, Yuan-Jie,Kim, Soo-Jin,Jeong, Young-Gil,Yu, Yun-Cho,Jo, Seung-Mook Korean Society of Electron Microscopy 2002 Applied microscopy Vol.32 No.4
We have detected the murine zinc transporter, ZnT3, and zinc ions in the mouse choroid plexus by immunocytochemistry (ICC) and zinc selenium autometallography ($ZnSe^{AMG}$), respectively. BALB/c mice served as experimental animals. Routine floating ABC immunocytochemical procedures were used for the ZnT3 immunocytochemistry, and the mice were injected intraperitoneally (i.p.) with sodium selenide (10 mg/kg) for the zinc selenium autometallography. The choroid plexus showed weak immunoreactivity (Ir) for ZnT3. At high magnification, ZnT3-Ir was seen to be located in the choroid epithelium and the connective tissue of the capillaries. At the EM level, a high electron density of ZnT3-immunoreactivity was restricted to vesicle membranes as well as microvilli in the apical membrane. In contrast, immunostaining of ZnT3 was completely absent in the basolateral plasma membrane and other cell organelles. After silver enhancement, fine $ZnSe^{AMG}$ grains were observed in both the epithelial and endothelial cells of the choroid plexus. Few $ZnSe^{AMG}$ grains present in the cell bodies of the choroid epithelial cells were located in multivesicular bodies. It is striking that very many $ZnSe^{AMG}$ grains were observed in the endothelial cells of the capillaries. These findings establish the choroid plexus as a non-neuronal pool of zinc ions in the brain, although the functional significance of this pool is not clear. The choroid epithelium, however, may play an important role in the transportation of zinc between the CSF and brain tissue. 본 연구는 BALB/c 생쥐 맥악얼기내 ZnT3 및 zinc 이온을 ZnT3 항혈청을 이용한 면역세포화학법(ABC법)과 autometallography ($ZnSe^{AMG}$)로 각각 동정하였다. 저배율에서 맥락얼기내 ZnT3 면역반응은 미약하였으나, 고배율에서는 맥락상피와 맥락조직에 국한된 뚜렷한 면역반응이 관찰되었다. 전자현미경에서 관찰된 ZnT3 면역반응은 주로 맥락상피의 자유면쪽 미세융모 및 막성 소기관에 국한되었던 반면 맥락조직내 모세혈관의 내피세포에서는 면역반응이 전혀 관찰되지 않았다. AMG 염색결과 맥락상피와 맥락조직에서 강한 AMG 과립이 관찰되었으며, 특히 모세혈관의 내피상피에서 가장 많이 AMG 과립이 분포하고 있었다. 맥락상피내 AMG 과립은 주로 다소포체에서 관찰되었으며, 소수는 특정 세포질소기관과 상관없이 사이토졸에 산재해 있었다. 이러한 본 연구의 결과를 바탕으로 저자들은 맥락얼기가 일종의 zinc pool의 역할이 있을 것이며, 최소한 뇌척수액과 뇌실질간에 zinc의 이동에 중요한 역할을 담당할 것으로 믿는다.
김성주,김경범,Kim, Sung Joo,Kim, Gyung Bum 한국반도체디스플레이기술학회 2021 반도체디스플레이기술학회지 Vol.20 No.1
In this paper, a method for improving the defect classification performance in low contrast, ununiformity and featureless steel plate surfaces has been studied based on deep convolution neural network and transfer-learning neural network. The steel plate surface images have low contrast, ununiformity, and featureless, so that the contrast between defect and defect-free regions are not discriminated. These characteristics make it difficult to extract the feature of the surface defect image. A classifier based on a deep convolution neural network is constructed to extract features automatically for effective classification of images with these characteristics. As results of the experiment, AlexNet-based transfer-learning classifier showed excellent classification performance of 99.43% with less than 160 seconds of training time. The proposed classification system showed excellent classification performance for low contrast, ununiformity, and featureless surface images.
퇴행성 아래눈꺼풀속말림의 치료에 있어 퀴커트 봉합술의 효과
배숭환,김명진,최용준,김성주,Soong-Hwan Bae,Myung-Jin Kim,Yong-Jun Choi,Sung-Joo Kim 대한안과학회 2005 대한안과학회지 Vol.46 No.7
Purpose: To evaluate the effectiveness of the Quickert-Rathbun suture to correct involutional entropion. Methods: A retrospective review was performed of 132 consecutive patients (132 lids, male 58, female 74) with involutional entropion who underwent correction from September 2001 to June 2004 at Kim`s eye hospital. All surgical corrections were performed with the Quickert-Rathbun suture technique. The average follow-up period was 9 months (1~17 months). Results: The average length of follow-up time was 14 months (6~22 months). There were no long-term complications like lagophthalmos or ectropion. Surgery was successful in 102 (77.2%) of the 132 eyelids in the first Quickert-Rathbun suture. The second Quickert-Rathbun suture was performed to 17 out of 30 recurred patients, One of whom was noted to have recurrent entropion at 12 months. The final success rate of Quickert-Rathbun suture was 89.3%. The average length of recurrence time was 9.1±8.4 months. Conclusions: The Quickert-Rathbun suture described is simple, straightforward and reliable for the correction of involutional entropion primarily.
성인에서 눈꺼풀속말림과 눈꺼풀겉말림 환자에서 발생원인과 치료방법에 대한 분석
강선아,박태성,이정혜,장재우,김성주,최혜선,Sunah Kang,MD,Tae Seong Park,MD,Jung Hye Lee,MD,Jae Woo Jang,MD,Sung Joo Kim,MD,Hye Sun Choi,MD 대한안과학회 2014 대한안과학회지 Vol.55 No.7
<b>Purpose:</b> To evaluate the causes and treatments of entropion and ectropion in adults. <b>Methods:</b> A retrospective review was performed of 397 patients (490 eyes) with entropion and 109 patients (138 eyes) with ectropion who underwent corrective surgery at Kim's Eye Hospital from January 2009 to December 2012. <b>Results:</b> The surgical correction of entropion was 3.5 times greater than that of ectropion. The causes of entropion were classified as senile (98.4%), cicatricial (1.4%) and spastic (0.2%). The causes of ectropion were classified as cicatricial (60.1%), paralytic (23.2%) and senile (16.7%). <b>Conclusions:</b> The main causes of entropion and ectropion were senile and cicatricial, respectively. Specifically, senile entropion was more common than senile ectropion, which is considered to be related to the lid anatomy of Asian patients. J Korean Ophthalmol Soc 2014;55(7):953-957