제48차 대한내과학회 추계학술대회초록집 : 심포지움 ; 급성심근경색 : 심근경색의 이차예방

김성구 ( Sung Koo Kim ) 대한내과학회 1996 대한내과학회지 Vol.51 No.-

제48차 대한내과학회 추계학술대회초록집 : 심포지움 ; 급성심근경색 : 심근경색의 이차예방

김성구 ( Sung Koo Kim ) 대한내과학회 1996 대한내과학회지 Vol.51 No.2S

CAD 프로그림을 이용한 돼지 성장호르몬 생산공정모사

김성구(Sung-Koo Kim),공인수(In-Soo Kong),공재열(Jai-Yul Kong),김영숙(Young-Sook Kim),박돈희(Don-Hee Park) 한국생물공학회 1995 KSBB Journal Vol.10 No.1

Chlorella saccharophila 배양을 위한 목질계 및 해조류 바이오매스 가수분해물의 이용

김아람 ( A-ram Kim ),김효선 ( Hyo Seon Kim ),박미라 ( Mi-ra Park ),김성구 ( Sung-koo Kim ),정귀택 ( Gwi-taek Jeong ) 한국미생물생명공학회(구 한국산업미생물학회) 2016 한국미생물·생명공학회지 Vol.44 No.4

본 연구에서는 3종류의 목질계 바이오매스와 4종류의 해조류 바이오매스를 전처리와 효소가수분해 과정을 통하여 얻은 환원당을 이용하여 C. saccharophila를 배양하였다. 얻어진 가수분해물을 활성탄 처리 유무에 따라 배양한 결과, 목질계 바이오매스와 해조류 바이오매스 가수분해물을 활성탄 처리하지 않은 경우에는 C. saccharophila의 성장이 저해 되었다. 목질계 바이오매스인 유채대와 보리대 그리고 볏짚과 해조류 바이오매스인 미역, 다시마, 파래 그리고 꼬시래기 가수분해물의 경우 활성탄 등으로 처리한 경우에서 목질계 바이오매스와 해조류 바이오매스 가수분해물에서 C. saccharophila의 성장이 관찰되었다. 미세조류의 생산성과 oil 함량, chlorophyll 등을 고려하였을 때 목질계 바이오매스와 해조류 바이오매스는 C. saccharophila의 product 생산을 위한 배양에 이점을 가지며, 특히 chlorophyll 생산에 보다 유용하리라 판단된다. In this study, we investigated the possibility of using hydrolysates of lignocellulosics (rapeseed straw, barley straw, rice straw) and marine macro-algae (Undaria pinnatifida, Laminaria japonica, Enteromorpha intestinalis, and Gracilaria verrucosa) to cultivate Chlorella saccharophila. The growth of C. saccharophila was inhibited by 7 hydrolysates without active carbon treatment. In contrast, hydrolysates treated with active carbon increased the cell growth and product (oil and chlorophyll) formation by C. saccharophila. The oil contents of C. saccharophila treated with each hydrolysate were 41.26 ± 0.69% (glucose), 22.06 ± 1.21% (rapeseed straw), 28.65 ± 1.08% (barley straw), 31.15 ± 0.76% (rice straw), 31.50 ± 2.12% (U. pinnatifida), 31.49 ± 4.53% (L. japonica), 29.63 ± 3.93% (E. intestinalis), and 26.15 ± 1.99% (G. verrucosa), respectively. Lignocellulosics and marine macro-algae may be useful resources for improving the mass cultivation of C. saccharophila.

성견 1면 골 결손부에서 Polylactic/polyglycolic acid copolymer (PLA/PGA) 흡수성 차단막의 치주 조직 재생에 대한 연구

김성구,황성준,김민경,김창성,이덕연,이용근,조규성,채중규,김종관,최성호,Kim, Sung-Koo,Hwang, Sung-Joon,Kim, Min-Kyoung,Kim, Chang-Sung,Lee, Doug-Youn,Lee, Yong-Keun,Cho, Kyoo-Sung,Chai, Jung-Kiu,Kim, Chong-Kwan,Choi, Seong-Ho 대한치주과학회 2003 Journal of Periodontal & Implant Science Vol.33 No.4

The goal of periodontal treatment is regeneration of the periodontium. Bone graft and absorbable PLA/PGA membrane have been used for this purpose. In this study, 4${\times}$4mm 1-wall intrabony defects were surgically created bilaterally in the mandible of five male beagles. The control group went through a conventional flap operation, while the experimental group I was treated with absorbable PLA/PGA membranes only, group II was treated with absorbable membrane and calcium phosphate. The results are the following : 1. The defect height was 4.82${\pm}$0.45mm in the control group, 4.93${\pm}$0.79mm in the experimental I group, and 4.92${\pm}$0.62mm in the experimental II group. There was no statistically significant difference among 3 groups(P <0.05). 2. The amount of junctional epithelium migration was 30.90${\pm}$9.92% of the defect height in the control group, 39.16${\pm}$7.51% in the experimental I group, and 38.68${\pm}$12.22% in the experimental II group. There was no statistically significant difference among 3 groups(P <0.05). 3. The amount of connective tissue adhesion was 36.38${\pm}$9.03% in the control group, 14.73${\pm}$3.93% in experimental I group, and 27.87${\pm}$9.70% experimental II group. Experimental group I was a statistically significantly different from control group(P <0.05). 4. The amount of new cementum regeneration was 32.92${\pm}$10.51%, 50.04${\pm}$7.61%, and 39.62${\pm}$12.14% for the control, experimental I, and experimental II group respectively. Experimental group I was a statistically significantly different from control group(P<0.05). 5. The amount of new alveolar bone regeneration was 27.24${\pm}$7.49%, 40.75${\pm}$8.03%, and 36.47${\pm}$15.11% for the control, experimental I, and experimental II group respectively. Experimental group I was a statistically significantly different from control group(P <0.05). The results suggest that the use of PLA/PGA membrane in 1-wall intrabony defect of beagle dogs may promote periodontal regeneration. Further studies are required to determine their regeneration effects.

포도당 수송자-1 결핍 증후군

김성구(Sung Koo Kim) 대한소아신경학회 2007 대한소아신경학회지 Vol.15 No.2

D-glucose is an essential fuel for metabolism in mammalian cells and the predominant fuel source for the brain. Transport of glucose across tissue barriers is mediated by stereospecific transporter proteins. Glut-1 is a major glucose transporter expressed on vascular endothelial cells comprising the blood brain barrier and is responsible for glucose entry into the brain. Impaired glucose transport across the blood brain barrier results in Glut-1 deficiency syndrome(DS). It is caused by haploinsufficiency of the blood brain barrier hexose carrier. Heterozygous mutations or hemizygosity of the GLUT-1 gene cause Glut-1 DS. It is characterized by infantile seizures refractory to anticonvulsants, developmental delay, acquired microcephaly, spasticity, ataxia, opsoclonus and other paroxysmal neurological phenomena, often occurring prior to meals. The diagnosis of Glut-1 DS is established in neurologically impaired patients with reduced cerebrospinal glucose concentration(hypoglycorrhachia) and lactate concentration in the absence of hypoglycemia. Decreased 3-O-methyl-D-glucose uptake in erythrocytes also supports the diagnosis of Glut-1 DS. Several treatment strategies have been pursued, none optimal, as it relates to the developmental encephalopahty associated with this clinical syndrome. Ketogenic diet has been effective in controlling seizures but has had little measurable effects on the associated cognitive impairments and behavioral disturbance. Current treatment is inadequate, and future studies should be directed at the mechanisms designed to upreglulate GLUT-1 expression, thereby increasing residual Glut-1 activity to 75 to 100%.

양어장수내의 암모니아성 질소제거를 위한 질화세균군의 고정화

김성구(Sung Koo Kim),서재관(Jae Koan Seo),이종석(Jong Seok Lee),공인수(In Soo Kong),서근학(Kuen hack Suh) 한국수산과학회 1997 한국수산과학회지 Vol.30 No.5

The immobilization of a microorganism has been rapidly progressed with the development of biotechnology in recent years. Although it has been used as a tool to isolate products from biological media in various areas, it has not yet been practiced in the treatment of waste water. In this paper, we suggest a possibility to apply the immobilization technique in the recirculating aquaculture system. We examined the ability of NH₄^+ removal by nitrifier consortium immobilized in Ba^(++)-alginate, κ-carrageenan and agar bead at the concentration of 50g/L, respectively. In order to use the immobilized nitrifier consortium as media in the fludized bed reactor, the strength of bead was measured. Ba^(++)-alginate as a support material showed higher strength of bead. Also, the nitrifier consortium immobilized in Ba^(++)-alginate showed higher nitrification activity that could remove 20㎎/L ammonium ion than those immobilized in other two support materials, carrageenan and agar. The immobilized nitrifier consortium showed better nitrification activity than free nitrifier consortium.

Changes in Hydrophobic Surface of Collagen by Chondroitin Sulfate : Fluorescence Intensity Measurements with Bis - ANS as the Probe

김성구(Sung-Koo Kim) 한국식품영양과학회 1995 한국식품영양과학회지 Vol.24 No.3

세포외 구조물질 (Extracellular Matrix, ECM)을 구성하고 있는 성분 중 가장 중요한 성분으로 콜라젠과 콘드로이친 설페이트를 들 수 있다. 콜라젠 분자의 소수성 아미노산 잔기에 의해서 나타내는 소수성 표면이 콜라젠 섬유의 굵기를 결정하는 요인으로 작용되고 있으며 아울러서 노화(aging)와 밀접한 관계를 가지고 있다. 세포외 구조물질의 구성 성분인 콘드로이친 설페이트와 콜라젠의 상호 작용에 의해 콜라젠 섬유의 굵기를 조절하는 기작을 소수성 발색단인 bis-ANS를 이용하여 측정하였다. 콘드로이친 설페이트에 의해서 가리워지는 콜라젠의 소수성의 표면적을 소수성 발색단으로 bis-ANS를 사용한 상대 형광도 측정으로 분석할 수 있었다. 콘드로이친 설페이트 첨가시 콜라젠의 상대 형광도는 콘드로이친 설페이트/콜라젠의 비가 3.8이 될 때 까지 감소하였다. 이 이상의 콘도로이친 설페이트의 농도 증가는 콜라젠의 상대 형광도에 아무런 영향도 미치지 못하였다. Rat tail 및 lathyritic rat skin 콜라젠에서도 비슷한 결과를 얻을 수 있었다. 콜라젠의 소수성 표면과 bis-ANS의 결합에 의해서 나타내어지는 상대 형광도는 pH에 의존적이었다. pH 6 이상의 콜라젠 용액에서 콘드로이친 설페이트에 의한 콜라젠 분자의 소수성 표면 shielding 효과를 확인할 수 없었다. 이는 높은 pH에서 콜라젠 분자의 음전하에 의한 반발력에 기인하는 것으로 추측할 수 있다. The important components of extracellular matrix(ECM) are collagen and chondroitin sulfate. The hydrophobic surface of collagen is one of the determining factors of diameter of collagen fiber and also is closely related to the aging phenomena. The controlling mechanism of the diameter of collagen fiber influenced by the interaction with chondroitin sulfate was evaluated using bis-ANS as a hydrophobic probe. Hydrophobic surface area of collagen molecule shielded by chondroitin sulfate was evaluated. Relative fluorescence intensity of collagen in the presence of chondroitin sulfate was measured using bis-ANS as a hydrophobic probe. The fluorescence intensity decreased with the increase in chondroitin sulfate up to 3.8 chondroitin sulfate / collagen (mole / mole). Further increase in the ratio of chondroitin sulfate to collagen did not change the fluorescence intensity. Similar changes in the relative fluorescence intensity were observed for both rat tail and lathyritic rat skin collagen. The fluorescence intensity indicated by the binding between bis-ANS and hydrophobic sites of collagen was pH dependent, and the shielding effect of collagen-chondroitin sulfate interaction could not be detected at pH above 6.0. This is probably due to the charge repulsions caused by negatively charged collagen molecules at higher pH.

고정화된 Nitrifier Consortium을 이용한 Total Ammonia-Nitrogen(TAN)의 제거

김성구(Sung-Koo Kim),공인수(In-Soo Kong),서재관(Jae-Koan Seo),김병진(Byong-Jin Kim),이민규(Min-Gyu Lee),서근학(Kuen-Hack Suh) 한국생물공학회 1997 KSBB Journal Vol.12 No.5

Chlorella saccharophila 배양 최적화 및 유용물질의 생산

김아람 ( A-ram Kim ),박미라 ( Mi-ra Park ),김효선 ( Hyo Seon Kim ),김성구 ( Sung-koo Kim ),정귀택 ( Gwi-taek Jeong ) 한국화학공학회 2017 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.55 No.1

본 연구에서는 Chlorella saccharophila의 배양을 통하여 바이오에너지 자원을 대량으로 확보하고자 배지 최적화 실험을 진행하였다. 최적화 인자로는 배양 형태, 초기 접종량, 탄소원 종류 및 농도, 질소원 종류 및 농도, 배양시간이다. 실험 결과, 배양 형태는 광원과 외부탄소원을 모두 공급하는 mixotrophic 배양이 적절하였다. 초기 접종량은 3% (v/v), 탄소원은 glucose 30 g/L, 질소원은 NaNO₃ 0.95 g/L를 첨가하는 것이 우수하였다. 최적 배지 조건으로 배양한 결과, oil의 함량은 12일에서 가장 높았으나, 회수되는 C. saccharophila의 biomass양과 chlorophyll의 양은 10일에서 가장 높았다. 위의 결과는 미세조류의 배지 최적화를 통하여 대량배양을 위한 기초자료로 사용될 수 있으리라 판단된다. In this study, the optimization of several factors for Chlorella saccharophila cultivation was investigated. The studied factors were medium type, culture type, inoculum size, sugar/nitrogen source type and concentrations. As a result, the optimized conditions for C. saccharophila cultivation were found to be the best at 3% (v/v) inoculum, 30 g/L glucose and 0.95 g/L NaNO₃ under mixotrophic culture. Under the optimized condition, the content of oil was high at 12 day, whereas, the amount of biomass and chlorophyll were high at 10 day.