만성 치주염 환자에서 2형 당뇨병이 $PGE_2$, MMP-14 및 TIMP의 발현양상에 미치는 영향

김선우,박진우,서조영,이재목,Kim, Seon-Woo,Park, Jin-Woo,Suh, Jo-Young,Lee, Jae-Mok 대한치주과학회 2007 Journal of Periodontal & Implant Science Vol.37 No.4

Purpose: The purposes of this study were to compare and quantify the expression of $PGE_2$, MMP-14 and TIMP-1 in the gingival tissues of patients with type 2 diabetes mellitus and healthy adults of chronic periodontitis with alveolar bone resorption. Material and methods: Gingival tissue samples were obtained during periodontal surgery or tooth extraction. According to the patient's systemic condition & clinical criteria of gingiva, each gingival sample was devided into three groups. Group 1 (n=8) is clinically healthy gingiva without bleeding and no evidence of bone resorption or periodontal pockets, obtained from systemically healthy 8 patients. Group 2 (n=8) is inflammed gingiva from patients of chronic periodontitis with alveolar bone resorption. Group 3(n=8) is inflammed gingiva from patients of chronic periodontitis with alveolar bone resorption associated with type 2 diabetes. Tissue samples were prepared and analyzed by Western blotting. The quantification of $PGE_2$ MMP-14 and TIMP-1 were performed using a densitometer and statistically analyzed by one-way ANOVA followed by Tukey test. Results: The expressions of MMP-14 and TIMP-1 were showed increasing tendency in group 2 & 3 compared to group 1. The expressions of $PGE_2$, MMP-14 were showed increasing tendency in group 3 compared to group 1 and group 2. According to MMP-14 levels were increasing, $PGE_2$ showed increasing tendency in group 3, and although $PGE_2$, MMP-14 levels were increasing, TIMP-1 levels were similar expressed comparing to group 2. Conclusion: In conclusion, this study demonstrated that the expression levels of MMP-14 and TIMP-1 had increasing tendency in inflammed tissue. It can be assumed that $PGE_2$ and MMP-14 may be partly involved in alveolar bone resorptive process and the progression of periodontal inflammation associated to type 2 DM.

치주인대세포 및 치은섬유아세포의 증식능에 대한 Epidermal growth factor의 영향

김선우,이재목,서조영,Kim, Seon-Woo,Lee, Jae-Mok,Suh, Jo-Young 대한치주과학회 1996 Journal of Periodontal & Implant Science Vol.26 No.4

Epidermal growth factor(EGF) is one of polypeptide growth factors. EGF has been reported as a biological mediator which regulates activities of wound healing process including the cell proliferation, migration and metabolism. The purposes of this study is to evaluate the effects of EGF on the human periodontal ligament cells and human gingival fibroblast cells that promote regeneration of periodntal tissue. The mitogenic effects of epidermal growth factor on human periodontal ligament cells and human gingival fibroblasts were evaluated by determining the incorporation of 5-Bromo-2'-deoxy-uridine into DNA of the cells in a dose dependent manner. The prepared cells were the primary cultured gingival fibroblast and periodontal ligament cells from humans, the fourth or sixth subpassages were used in the experiments. Cells were seeded in DMEM containing 10% FBS. 1, 10, 50, 100, $200{\eta}g/ml$ and epidermal growth factor were added to the quiescent cells for 24 hours, 48 hours and 72 hours. They were labeled with $10\{mu}l/200{\mu}l$ 5-Bromo-2'-deoxy-uridine for the last 6 hours of each culture. The results of the five determinants were presented as mean and S.D.. The results were as follows : The DNA synthetic activity of human gingival fibroblasts were increased dose dependently by epidermal growth factor at 24 hours, 48 hours and 72 hours. The mitogenic effects were similar at the 24 and 48 hours of epidermal growth factor, but the DNA synthetic activity of human gingival fibroblasts generally decreased at 72 hours. The DNA synthetic activity of human periodontal ligament cells were increased dose dependently by epidermal growth factor at 24 hours but the DNA synthetic activity decreased at $200{\eta}g/ml$ of each hour. Generally the maximum mitogenic effects were observed at the 48 hours application of epidermal growth factor. The DNA synthetic activity of human periodontal ligament cells generally decreased lower at 24, 72 hours than at 48 hours the application of epidermal growth factor. In the comparison of DNA synthetic activity between human gingival fibroblasts and human periodontal ligament cells, human periodontal ligament cells had slightly higher proliferation activity than human gingival fibroblasts for a longer time at the high dosage of the epidermal growth factor. In conclusion, epidermal growth factor have important roles in the stimulation of DNA synthesis in human periodontal ligament cells and human gingival fibroblasts, and thus may be useful for clinical applications in periodontal regenerative procedures.

위암 환자의 위절제술 후 식도산도의 변화와 운동장애

김선우,이상호,Kim Seon-woo,Lee Sang-Ho 대한위암학회 2004 대한위암학회지 Vol.4 No.4

Purpose: Some patients develop gastroesophageal reflux disease (GERD) after a gastrectomy for stomach cancer. Therefore, we conducted this research to gain an understanding of esophageal acidity and motility change. Materials and Methods: From July 2002 to March 2004, the cases of 15 randomized patients with stomach cancer who underwent a radical subtotal gastrectomy (RSG) with Billroth I(B-I) reconstruction (n=12) or a radical total gastrectomy (RTG) with Roux-en-Y (R-Y) gastroenterostomy (n=3) were analyzed. We investigated the clinical values of the ambulatory 24-hour pH monitoring and esophageal manometry in these patients, just before discharge from the hospital after an operation. Results: GERD was present in three patients ($20\%$). Compared with two reconstructive procedures, 3 of the 12 patients in the RSG with B-I group had GERD; however, none of RTG with R-Y group had GERD. Compared with pathologic stage, 2 of 9 patients in stage I, 1 of 2 patients in stage II, none of 3 patients in stage III, and none of 1 patient in stage IV had GERD. Esophageal manometry was performed in 10 patients. Nonspecific esophageal motility disorder (NEMD) was present in 7 patients. Conclusion: Some patients had GERD as a complication following a gastrectomy for stomach cancer. We suspect that the postoperative esophageal symptom is due to not only bile reflux but also gastroesophageal acid reflux. Therefore, careful observation is recommended for the detection of GERD. 목적: 위암 환자의 위절제술 후 발생하는 가슴 쓰림, 역류, 비심인성흉통 등 식도증상의 원인규명을 위해 위절제술 후 식도 산도 변화와 운동장애를 알 수 있는 24시간 보행성 식도 산도 검사(일부 환자에서 식도 내압 검사를 병행)를 실시하였다. 대상 및 방법: 2002년 7월부터 2004년 3월까지 위암으로 Billroth I을 시행한 환자와 위전절제술을 시행한 환자 중 무작위로 선택한 15예를 대상으로 술 후 음식 섭취 후 퇴원 전에 24시간 보행성 식도 산도 검사를 시행하였다. 산 역류의 양성 판정은 DeMeester 점수를 사용하여 점수 14.72 이상을 양성으로 하였고, 증상지수는 증상을 보인횟수 중 실제로 산도(pH)가 4 이하였던 경우를 나타내는 것으로 양성판정은 증상지수 $50\%$ 이상인 경우로 하였다. 결과: 무작위로 선택되어 검사한 15예 중 산역류양성 (DeMeester 14.72 이상)을 보인 예는 총 3예($20\%$)로 분석 되었다. 그 중 술 식에 따른 분포는 Billroth I경우는 총 12예 중 3예($25\%$)에서 양성반응을 보였고 위전절제술을 시행한 환자는 3예 중 양성반응을 보인 예가 없었다. 병기에 따른 분포는 각각 I (2예/9예), II (1예/2예), III (0예/3예), IV(0예/1예)였다. 10예에서 식도 내압 검사를 병행하였는데 그 중 7예에서 비특이적 식도운동장애로 분석되었다. 결론: 위암 환자의 위절제술 후 산 역류로 인한 위식도 역류질환이 발생했다. 위암수술로 Billroth I 재건술 후 생기는 위식도역류 증상은 담즙역류뿐만 아니라 위산 역류에 의해서도 생긴다.

문태준 『맨발』에 나타난 ‘동물’ 표상 연구 -김준오 『시론』의 ‘동일화의 원리’를 적용하여-

김선우 ( Kim Seon-woo ) 한국문학이론과 비평학회 2023 한국문학이론과 비평 Vol.98 No.-

문태준 시 연구의 공통점은, 그의 ‘기존의(전통적·향토적) 서정시’적 특징을 인정하면서 그것을 시 해석의 토대로 삼고 있다는 지점이다. 그리고 이를 바탕으로, 문태준만의 서정성을 면밀하게 검토하고 있다는 것이다. 그러나 서정시 특유의 문법 및 원리에 천착해 들어가 ‘서정(시)의 세계’를 그리는 문태준 시의 적절함을 확인하였는지 미지수이다. 따라서, 본 논문은 이전의 논의들이 다룬 문제·주제 의식을 포괄하면서도, 그것을 넘어서는 문태준 시의 ‘기존의 서정시’적 특징에 집중해보고자 한다. 본 연구에서는 김준오의 『시론』을 적용해 논의를 개진하고자 한다. 김준오는 서정시를 ‘자아와 세계의 동일화’라고 설명하고, ‘동일화의 원리’인 ‘동화’와 ‘투사’를 통해 그것을 추구할 수 있다고 덧붙인다. 문태준의 시에 나타나는 동화와 투사는 곧, 시인의 정서 작용을 나타내는 지표라고 할 수 있다. 본 논문에서는, 문태준의 시에서 가장 두드러지는 표상이지만 섬세하게 다루어지지 못한 ‘동물’을 통해 논의를 개진하고자 한다. 문태준 시의 ‘동물’은 갈등과 대립, 배제와 분별의 원리가 가동되는 현실의 세계, 타자의 세계에 적극적으로 배치되는 가능의 세계, 서정의 세계를 표시해준다. ‘동화’와 ‘투사’의 원리는 각각, ‘동물’ 형상화를 통해 ‘용해와 통합의 세계’, ‘이입과 발견의 세계’를 보여준다. 본 논문을 통해 추수할 수 있는 몇 가지의 유의미를 정리해보자면 다음과 같다. 첫째, 서정시의 ‘동일화의 원리’인 ‘동화’와 ‘투사’를 적용하여 시를 분석함으로써, 향후의 ‘서정시’에 관한 여러 고찰에 참조가 되어줄 수 있을 것이다. 둘째, 서정시, 생태시, 불교시의 특질은 문태준의 시에서 두드러지는데, 특히 ‘서정시’를 활용해 시 세계를 고찰함으로써 ‘생태시’와 ‘불교시’의 논의에 근거로서 보탬이 될 수 있을 것이다. 그리고 셋째, ‘동물’ 이미지를 살핌으로써, ‘동물’의 재현과 형상화 양상을 살피는 당대의 논의에 다소간 보탬이 되어줄 수 있을 것이다. Studies of Moon Taejun's poems have something in common that they recognize the characteristics of his ‘existing (traditional and local) lyric poetry’ characteristics and use them as the basis for poetry interpretation. And based on this, it is closely examining the lyricism of Moon Taejun. However, it is unclear whether the appropriateness of Moon Taejun’s poem, which draws the ‘world of lyric poetry,’ has been confirmed by entering deeply into the grammar and principles unique to lyric poetry. Therefore, this study aims to focus on the ‘existing lyric’ characteristics of Moon Taejun’s poems, which include the sense of problem and thematic consciousness covered by previous discussions, but go beyond it. This study aims to present a discussion by applying Kim Joonoh’s “Poetics.” Kim Joonoh explains lyric poetry as ‘the identification of the self and the world,’ and adds that it can be pursued through ‘the principle of identification,’ ‘assimilation’ and ‘projection.’ The assimilation and projection in Moon Taejun’s poems can be seen as indicators of the poet’s emotional action. This study discusses ‘animals’ that are the most prominent representations of Moon Taejun’s poems but are not demonstrated delicately. Moon Taejun’s ‘animal’ represents the real world where the principles of conflict and confrontation, exclusion and discrimination are operated, the possible world that is actively deployed to the world of others, and the lyric world. The principles of ‘assimilation’ and ‘projection’ show ‘the world of dissolution and integration’ and ‘the world of introduction and discovery’ through the figuration of ‘animals’, respectively. The following is a summary of some of the significance of this paper. First, poetry was analyzed by applying the ‘assimilation’ and ‘projection,’ which are the principles of identification in lyric poetry, and it can be a reference to various studies of ‘lyric poetry’ in the future. Second, the characteristics of lyric poetry, ecological poetry, and Buddhism poetry stand out in Moon Taejun’s poems, which can contribute to the discussion of ‘ecological poetry’ and ‘Buddhism’ by using ‘lyric poetry’ to examine the world of poetry. And third, this study looked at the image of ‘animal,’ which could contribute to the discussion of the time to examine the reproduction and the aspect of figuration of ‘animal.’

주요가뭄영향인자 도출을 위한 특징선택 기법 적용

김선우(Kim, Seon Woo),이희수(Lee, Hee Soo),김영식(Kim, Yeong Sik),정동휘(Jung, Donghwi) 대한토목학회 2019 대한토목학회 학술대회 Vol.2019 No.10

탈성매매 여성이 성매매 중 겪은 학대와 탈성매매 이후 정서조절 곤란의 관계

김선우(Kim, Seon-Woo),김근향(Kim, Keun-Hyang) 한국웰니스학회 2020 한국웰니스학회지 Vol.15 No.1

본 연구는 탈성매매 여성을 대상으로 성매매 중 겪은 학대 경험과 탈성매매 이후의 정서조절 곤란의 관계를 알아보기 위한 것으로, 성매매 기간에 신체적, 성적, 심리적 학대와 정서조절 곤란의 관련성을 확인하였다. 성매매 기간 중 학대는 학대 빈도와 강도를 합한 학대 심각도로 정의하였다. 학대 심각도 고 집단은 학대 빈도를 한 번 이상 보고한 사람 중 학대 강도에서 집단의 평균 점수 이상으로 나타난 여성으로, 학대 심각도 저 집단은 학대 빈도를 보고하지 않은 여성과 학대 강도에서 집단의 평균 점수 이하로 나타난 여성으로 구성하였다. 연구결과, 심각한 학대를 경험한 집단은 그렇지 않은 집단과 정상 통제집단 보다 대부분의 정서조절 곤란 지표에서 유의하게 높은 점수를 보였다. 그러나 정서 주의/자각 요인의 경우, 성매매 중 심각한 학대를 경험한 집단과 그렇지 않은 집단 간 유의한 차이는 나타나지 않았다. 이에 탈성매매 여성은 성매매 중 심한 학대를 경험할수록 전반적인 정서조절 능력의 어려움이 상당하지만, 자신의 정서에 주의를 기울이고, 자각하는 능력의 결함은 성매매 중 학대 심각도에 상관없이 모든 탈성매매 여성들에게서 동일하다는 점이 시사된다. 그 외 본 연구의 제한점과 의의는 논의에서 다루어졌다. This purpose of this research was to investigate the relationship of abuse during prostitution and emotion dysregulation in women who had been out of prostitution. Specifically, the relationship between the severity of physical, sexual and psychological abuse during prostitution and emotion dysregulation was explored. For this, experience of being abused during prostitution was defined as abuse severity that abuse frequency and strength are combined. The group with high abuse severity was composed of women who reported abuse severity scores over the average among those who had been abused at least once. On the other hand, the group with low abuse severity consisted of women who did not report having been abused and those who reported abuse severity scores below the average. As a result, the high-severity group showed significantly higher scores in dysregulation of impulse control, emotional non-acceptance, lack of emotional clarity, limited access to emotion regulation strategies and lack of goal-directed behavior compared to the low-severity group and the control group. However, emotional awareness factor among sub-factors of emotion dysregulation did not show significant difference between the high-severity group and the low-severity group. The implication and limitations of this research were explained in Discussion.

특징선택기법을 이용한 가뭄영향인자 분석

김선우(Kim, Seon Woo),김민기(Kim, Min Ki),정동휘(Jung, Donghwi),유도근(Yoo, Do Guen) 한국방재학회 2019 한국방재학회논문집 Vol.19 No.7

일반적으로 가뭄은 다른 자연재해보다 광범위한 지역에 오랜 피해를 입힌다. 최근에는 10년 이상, 수십 년 동안 지속되며 전국 범위에 피해를 끼칠 수 있는 “메가가뭄”의 발생 가능성이 인지되고 있다. 하지만, 이러한 극한가뭄의 결과에 영향을 미치는 기상학적, 수문학적, 사회-경제적 영향요인에 대한 정량적 분석이 미비하였다. 본 연구는 총 9개의 기상학적(강수량, 평균기온, 평균풍속, 상대습도, 평균기압, 일조율), 수문학적(저수위, 댐방류량), 사회·경제적 인자(생활용수사용량)와 가뭄지수간의 상호관계를 정량화하여 가뭄영향인자를 도출하고 직·간접적 영향분석을 수행하였다. 통계학적 기법 기반 특징선택모형인 선형판별분석과 주성분분석을 사용하였으며, 가뭄의 심도는 표준강수지수(Standardized Precipitation Index, SPI)를 이용하였다. 연구 대상 지역으로 충청남도 보령시를 선정하였다. 특징선택기법별, 이용 가뭄지수 종류별 결과를 방사형 차트(Radar chart)를 이용하여 도시 및 비교하였다. 선형판별분석과 주성분분석을 각각 적용하여, 보령댐 저수위가 가뭄에 가장 큰 영향을 주는 인자임을 확인하였다. Drought generally affects a wider area for longer period of time than other natural disasters. Recently, so-called mega-droughts of very long durations and with more extensive impacts have been observed across the world. However, few efforts have been devoted to identifying the critical impact factors-such as climate, hydrological and socio-economic factors-for such extreme droughts. In this study, we identify the most critical factor and derive the direct/indirect relationship among nine influences including climatic, hydrological, and socio-economic factors using two feature selection methods. The two feature selection approaches we use are based on the statistical theories of principal component analysis and linear discriminant analysis. The standardized precipitation index (SPI) is used as a drought index. The study area is Boryeong city, South Korea. Drought impact analysis results obtained from the two feature selection approaches are compared and presented by polygon radar charts. The results showed that the low water level of the Boryeong dam is the most critical impact factor.

Research ABC : Statistical errors in medical research

김선우 ( Seon Woo Kim ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2

Today, statistics is widely used as a powerful tool in the scientific research process. Statistics is based on mathematics, and provides scientific and efficient experimental design as well as objective evidence, if the method is appropriate. This fact should be the reason to use statistics, but statistics is often used for the tool to publish article or for funding. This results in misuse of statistics, and leads to serious problems specially in medical research. In reviewing articles, statistical errors to be missed, for example no correction of p-value in the presence of multiple testing, become to be criticized. In this talk, common pitfalls and corresponding solutions, in statistical view, are addressed with statistical guidelines for manuscript. Real examples with statistical errors and reviewer``s comments are also presented. This presentation emphasizes consistency of study objectives, hypotheses, design of the study, groups for comparisons, endpoints, type of data, statistical methods, and interpretation of statistical results. Details for descriptive statistics, categorization, multiple testing problems, subgroup analysis, multivariable analysis, effect size, p-value and confidence interval etc. are presented with some examples.

SF₆의 플라즈마 분해로부터 생성된 입자상 물질에 관한 연구

김선우(Seon-Woo Kim),김종범(Jong-Bum Kim),김재환(Jae-Hwan Kim),김래형(Rae-Hyeong Kim),류재용(Jae-Yong Ryu) 한국대기환경학회 2017 한국대기환경학회지 Vol.33 No.4

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