계배 대뇌의 신경세포 분화에 미치는 수은의 영향 ( I )

김생곤,조광필,김정상,Kim, Saeng-Gon,Cho, Kwang-Phil,Kim, Jeong-Sang 한국현미경학회 1994 Applied microscopy Vol.24 No.2

To investigate the effects of mercuric chloride ($HgCl_2$) on the differentiation in the cerebral neuron of chick embryo 7 days, the ultrastructural changes in nerve cells injected with a various doses of mercuric chloride were observed with transmission electron microscope. The enzyme activity of the some dehydrogenases, and adenosine triphophate (ATP) were also analyzed. The results obtained are as follows; The ultrastructural changes in 1.0mg-injected group, the nuclear envelope were irregular, and the RER, Golgi complexes and mitochondria were not well developed. In 2.0mg-injected group, the nuclear envelope were partly destroyed or detached, and mitochondria were decreased in number and their cristae were destroyed, too. The RER and Golgi complexes were less developed than those of the normal groups. In general, the activities of dehydrogenases were declined by increasing the dose of mercuric chloride. Lactate dehydrogenase (LDH) activity fatted to below 85% of the normal group in 1.0mg-injected group, and 69% in 2.0mg-injected group. Malate dehydrogenase (MDH) activity was decreased greatly to 76% in 2.0mg-injected group. Succinate dehydrogenase (SDH) activity fatted to 85% in 1.0mg-injected group, and 74% in 2.0mg-injected group. ATP content in 1.0mg-injected group was almost near to the normal level, but it was increased significantly in 2.0mg-injected group.

Cyclophosphamide가 흰쥐의 부정소에 미치는 영향 I. 두부

조광필,김생곤,정해만,김정상,김영곤,노영복,Cho, Kwang-Phil,Kim, Saeng-Gon,Jung, Hae-Man,Kim, Jeong-Sang,Kim, Young-Gon,Rho, Young-Bok 한국현미경학회 1992 Applied microscopy Vol.22 No.1

This research was undertaken to determine the effects of the anticancer and immunosuppressive drug cyclophosphamide (CP) on the epididymal caput of the male rat in terms of ultrastructural alteration and protein analysis by SDS-PAGE at different groups; control group, 1 week group, 3 weeks group, 5 weeks group were treated with saline (control group) or CP at doses of 20 mg/kg/week, 1 time a week, respectively. In the cytoplasm of the principal cells on the epididymis, the mitochondrial outer and inner membranes were significantly swollen or disrupted. The lumens of rough endoplasmic reticulum (rER) were also swollen, and the number of Golgi vesicles were increased, respectively. It is suggested that treatment with CP alters the specific cell organelles in the epididymis. CP caused changes in protein concentrations in caput of epididymis after CP treatment. Total proteins of 32 to 37 species such as lactate dehydrogenase, carnitine acetyltransferase and succinate dehydrogenase were expressed in the caput fluid. Then the more CP was increased, the more concentration of proteins caused to decrease, synthesize or increase in epididymis. In contrast to the control group, in particular carnitine acetyltransferase and the other 9 proteins in the caput fluid were decreased or disappeared, respectively, whereas lactate dehydrogenase and the other 5 proteins in the caput fluid were increased or synthesized, respectively. The other proteins are not showed distinctive difference. These alterations could be direct mediated by toxic effects of the drug on the epithelium or be secondary to changes in the spermatozoa as a result of the CP treatment.

분자생물학적 기법을 이용한 악골 골수염 병소의 세균 동정

김미성(Mi-Sung Kim),김수관(Su-Gwan Kim),정해만(Hae-Man Chung),김생곤(Sang-Gon Kim),국중기(Joong-Ki Kook),김미광(Mi-Kwang Kim),김화숙(Hwa-Sook Kim),유소영(So Young Yoo) 대한구강악안면외과학회 2003 대한구강악안면외과학회지 Vol.29 No.1

The purpose of this study was to isolate and identify the bacteria in osteomyelitis lesion of 3 patients. Two lesions were due to the post-infection after extraction. The other was resulted from mal-fixation of both sides of mandibular angles. Pus samples were collected by needle aspiration from the lesion and examined by culture method. Bacterial culture was performed in three culture systems (anaerobic, CO2, and aerobic incubator). Identification of the bacteria was performed by 16S rRNA gene cloning and nucleotide sequencing method. Our results showed that Streptococci species was predominantly isolated in both lesions of extraction socket. Only one species (Proteus vulagris) was detected in lesion of mandibular angle. This study was not sufficient to identify the causative bacteria in those osteomyelitis. However, our data may be offered the clue to solve the problem.

Effect of Alginate on Early Bone Healing after Implantation of Particulate Dentin and Plaster of Paris Mixture

조경안,김수관,임성철,김생곤,Cho, Gyung-Ahn,Kim, Su-Gwan,Lim, Sung-Chul,Kim, Sang-Gon Korean Association of Maxillofacial Plastic and Re 2005 Maxillofacial Plastic Reconstructive Surgery Vol.27 No.3

본 연구의 목적은 치아 회분말과 연석고 및 알긴산을 혼합하여 이식한 후 초기 골 형성을 알아보는 데 있다. 백서 30마리의 두개골에 8mm 직경의 골결손부를 형성한 후 인간의 치아 회분말과 연석고 및 알긴산을 혼합하여 이식한 후 대조군, 치아 회분말과 연석고 처치군, 그리고 치아 회분말과 연석고 및 알긴산 혼합 처치군에서 각각의 골 결손부 치유과정을 조직병리학적, 조직형태계측학적으로 비교 관찰하였다. 통계학적으로 주간 변화(4주, 8주)는 유의하지 않았고, 각 그룹 간에 있어도 치아 회분말과 연석고 처치군과 치아 회분말과 연석고 및 알긴산 혼합 처치군 간 모두(4주, 8주) 유의한 차이가 없었다. 그러나 이들 치아 회분말과 연석고 처치군과 치아 회분말과 연석고 및 알긴산 혼합 처치군은 대조군에 비해서는 유의하게 우수한 신생골 형성을 보였다. 비록 통계학적으로는 치아 회분말과 연석고 처치군과 치아 회분말과 연석고 및 알긴산 혼합 처치군이 유의한 차이를 보이지 않았지만 치아 회분말과 연석고 및 알긴산 혼합 처치군에서 보다 더 우수한 신생골 형성 경향을 보였다. 뿐만 아니라, 신생골의 내용면에서도 치아 회분말과 연석고 및 알긴산 혼합 처치군에서 좀더 많은 신생 직조골의 융합이나 골 소주형성이 관찰되어 신생골의 형성 및 성숙에 알긴산이 역할을 담당하는 것으로 사료되었다. 결론적으로 골결손부 치료 방법으로 치아 회분말과 연석고 처치군 또는 치아 회분말과 연석고 및 알긴산 혼합 처치로 결손부에 이식하는 것은 신생골 형성의 양적, 질적 개선에 통계학적으로 유의하며, 알긴산을 이식재와 함께 처리한 경우에 신생골 형성의 양적, 질적 개선에 도움을 줄 수 있을 것으로 사료된다.$1^{\circ}C$ 냉수로 세척하고 PETE tray로 포장하여 $4^{\circ}C$로 저장한 경우 깻잎 고유의 초록색과 향을 유지하고 있어 저온냉수 세척과 tray 포장이 세척 청경채의 선도 유지에 효과가 있는 것으로 나타났다.1%,\;pendimethalin\;1.3{\sim}2.9%$ 및 $3.8{\sim}10.8%,\;ethoprophos\;0.6{\sim}2.7%$ 및 $0.1{\sim}0.3%$이었다. 인공강우실험 후 공약의 토심별 분포를 살펴 본 결과 alachlor와 ethopropho는 토심 $10{\sim}15cm$까지 이동하였고, ethalfluralin과 pendimethalin는 대부분 토심 5 cm 이내에 잔류하였다. 경사도 30%의 경우가 10%에 비하여 각 농약의 유실량이 $0.2{\sim}1.9$ 배 증가하였는데 유출수에 의한 농약의 유실량 차이는 유출수 중 농도 차이로 판단되며, 유실토양에 의한 농약 유실량 차이는 토양 유실량과 관계되는 것으로 생각되었다. 농약의 강우에 의한 유실은 복잡하게 작용하는 많은 환경적 요인에 의하여 영향을 받지만 정교하게 구성된 환경 시나리오에 의하여 예측 가능할 것으로 판단되었다.고 도라지는 물에 우려 푹 삶았고, 감자, 송이 등은 잘게 썰어 쌀과 함께 밥을 조리하였다. 4. 약선 음식조리방법 약선음식의 재료는 평상시 식생활에 사용되고 있던 식품들의 기능성분과 약이성을 이용하여 만성적인 질병과 급성적인 복통 설사 등에 재료의 전처리를 통해 죽으로 많이 이용하였다. 특히 곡류 등은

가토에서 하악골 신연 6주후 신연양에 따른 두개하악관절의 조직병리학적 변화

김현호(Hyun-Ho Kim),김수관(Su-Gwan Kim),임성철(Sung-Chul Lim),정해만(Hae-Man Chung),김생곤(Sang-Gon Kim) 대한구강악안면외과학회 2003 대한구강악안면외과학회지 Vol.29 No.2

The purpose of this study is to observe histopathologic changes in the bilateral craniomandibular joints after allowing 6 weeks of consolidation by varying the amount of distraction in rabbit mandible. Eight rabbits weighing about 2 to 3 kg were used. After corticotomy was performed on the left mandibular body between the first premolar and the second premolar region, a unilateral fixation device was placed. Then, a 7-day period was allowed without distraction of the device. The mandible was lengthened 0.5 mm/day. Corticotomy and lengthening of mandible were not performed in control group. After the completion of the lengthening process, a 6-week-consolidation period was allowed. Then, the rabbits were sacrificed, and histologic examination of the craniomandibular joints was performed. Proliferative changes were observed in the craniomandibular joints in all groups. With the increasing amount of distraction, hypertrophy of the cartilage layer became more severe, bone formed was dense and enchondral ossification was clearly shown in subchondral bone. Hypertrophy of the cartilage layer was also seen in the non-distracted side as the distracted side in the experimental group. These results indicate that when physical force is applied constantly to joints, the proliferation of articular cartilage and bone formation are present. When more than 6 weeks of consolidation period is allowed at the time of performing distraction for more than 5 mm, articular changes, especially, in the contralateral side should also be noted.

(-)-Epicatechin의 치주질환원인균에 대한 항균 효과

이정은 ( Jeong Eun Lee ),배슬아 ( Seul A Bae ),김생곤 ( Sang Gon Kim ) 조선대학교 구강생물학연구소 2006 口腔生物學硏究 Vol.30 No.2

(-)-Epicatechin의 치주질환원인균에 대한 항균 효과

이정은 ( Jeong Eun Lee ) , 배슬아 ( Seul A Bae ) , 김생곤 ( Sang Gon Kim ) 조선대학교 구강생물학연구소 2006 Oral Biology Research (Oral Biol Res) Vol.30 No.2

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