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수컷 SD(rat)에 $CCI_4$ 처리후 지구자 추출액의 간 보호효과 연구
김상웅,마진열,Kim, Sang-Woong,Ma, Jin-Yeul 한국한의학연구원 2006 한국한의학연구원논문집 Vol.12 No.1
We investigated liver protection effect of the Hovenia dulcis Thunb extraction which has treated with carbon tetrachloride ($CCl_4$, 1.0 ML/kg) in SD male rat (20 weeks). We observed the amount of leukocyte, erythrocyte, hemoblobin and hematocrit are increased and the number of plaque is decreased for treatment of $CCl_4$, However, after treatment of Hovenia dulcis Thunb extraction (15 mL/kg), we have same result that of control group which has treated with $CCl_4$ The blood biological data showed that the value of AST (Aspartate aminotransferase), ALT(Alanine aminotransferase) and LDH (Lactate dehydronase) are increased significantly for the negative control group compared to that of control group. For experimental group, respectively, In addition, it was confirmed that the recovery effect of a mysterious death of an interstitial cell of the experimental group is to be compared to that of the negative control group for a sample of pathological tissue.
철도차량용 축전지 적용 사례 (Cases of applying battery to rolling stock)
김상웅(Sang-Woong Kim),김신국(Shin-Gug Kim),안홍관(Hong-Kwan Ahn),김재기(Jea-gi Kim) 한국철도학회 2011 한국철도학회 학술발표대회논문집 Vol.2011 No.5
축전지는 차량을 기동할 때나 비상시의 제어전원으로서 철도차량의 구성품 중 매우 중요한 부분이다. 현재 널리 사용되고 있는 축전지로는 연축전지와 니켈카드뮴축전지 등이 있으며 점차 소형화 경량화 되어 가고 있는 추세이다. 또한 철도차량의 전자제어기술의 발달로 인하여 필요로 하는 전기용량이 늘어나고 있다. 따라서 다양한 종류의 고효율 축전지들이 신규 노선의 차량에 검토되고 있지만 철도차량용 축전지는 안전상으로 충분히 검증이 되어야 하며 또한 관리 측면에서도 어려움이 없어야 하는 요구조건으로 인하여 실제 적용하는 데에는 많은 어려움이 있다. 본 논문에서는 현재 철도차량에 적용된 축전지 사례를 조사하고 적용을 시도 중인 축전지들의 특성을 확인하여 안전 및 성능, 관리적인 측면에서 어떠한 문제가 있는지 검토하여 향후 축전지 발전 동향에 대하여 논하고자 한다. A battery is an important part of the component. as a power source of the control of rolling stock at starting movement or emergency control. Now widely used batteries are Ni-Cd batteries and lead accumulators, and these are increasingly getting smaller and lighter. In addition, the electric capacity required is increasing. due to the development of electronic control technology of rolling stock. Therefore, various kinds of high-efficiency battery are considered for the new routes’ rolling stock, but rolling stock’s batteries should be fully tested to prove safety and also have no difficulty in terms of management. so because of the requirement. it is difficult to be in practical application. In this paper, we will survey cases of applying battery to rolling stock and then we will review whether there is any problem about safety and performance, management to discuss future trend of batteries.
탐색적 자료 분석 및 연관규칙 분석을 활용한 잔류농약 부적합 농업인 유형 분석
김상웅 ( Sangung Kim ),박은수 ( Eunsoo Park ),조현정 ( Hyunjeong Cho ),홍성희 ( Sunghie Hong ),손병철 ( Byungchul Sohn ),홍지화 ( Jeehwa Hong ) 한국품질경영학회 2021 품질경영학회지 Vol.49 No.1
Purpose: The purpose of this study was to analysis pattern of nonconforming farmers who is one of the factors of unconformity in residual pesticides. Methods: Pattern analysis of nonconforming farmers were analyzed through convergence of safety data and farmer’s DB data. Exploratory data analysis and association rule analysis were used for extracting factors related to unconformity. Results: The results of this study are as follows; regarding the exploratory data analysis, it was found that factors of farmers influencing unconformity in residual pesticides by total 9 factors; sampling time, gender, age, cultivation region, farming career, agricultural start form, type of agriculture, cultivation area, classification of agricultural products. Regarding the association rule analysis, non-conformity association rules were found over the past three years. There was a difference in the pattern of nonconforming farmers depending on the cultivation period. Conclusion: Exploratory data analysis and association rule analysis will be useful tools to establish more efficient and economical safety management plan for agricultural products.
Effect of Growth Conditions in the Attachment of Salmonella typhimurium to the Host Cells
김영희,김상웅,강호영,Kim, Young-Hee,Kim, Sam-Woong,Kang, Ho-Young Korean Society of Life Science 2005 생명과학회지 Vol.15 No.4
Salmonella typhimurium TML이 Int-407 숙주세포에 부착하는 정도는 S. typhimurium이 낮은 농도의 철이 함유된 배지에서 배양되었을 때보다 LB 액체배지 또는 높은 농도의 철이 함유된 배지에서 생육한 것에서 약 10배 정도의 높은 수준으로 관찰되었다. 고농도의 철이 포함된 배양조건이 살모넬라가 숙주세포에 부착시키는 정도를 향상시키는데 반해, 칼슘, 코발트, 구리, 인산, 마그네슘 그리고 망간과 같은 다른 양이온은 그렇지 않다. 이것은 아마도 철이 Salmonella가 부착에 필요한 요소들의 발현을 활성화하는 역할을 하는 것으로 추정된다. 철 농도에 따른 부착정도의 차이들은 type 1 fimbriae, mannose resistant hemagglutinin과 flagellum등을 생성하지 않는 다양한 S. typhimurium 돌연변이주들에서도 관찰되는 것으로 미루어보아 이들 구조체들과 상관이 없는 밝혀지지 않은 어떤 인자가 부착성 증가에 관여하는 것으로 사료된다. fur유전자가 불활성화된 S. typhimurium 돌연변이주의 부착성이 야생형 Salmonella와 유사한 방식으로 철에 의해 조절되었는데, 이는 철 농도에 따른 부착성의 변화에 관여하는 잠재적인자의 발현이 Fur 단백질과 독립적으로 이루어진다는 것을 나타낸다. An approximately 10-fold higher level of adherence of Salmonella typhimurium strain TML to Int-407 cells was observed with organisms grown in Luria broth or in high-iron containing medium than those grown in low-iron containing medium. Iron specifically enhanced adherence, while other cations such as calcium, cobalt, copper, potassium, magnesium and manganese did not. It was suggested that iron did not act as a passive ligand - probably it stimulated production of bacterial factors necessary for adherence. A similar pattern of iron modulation of adhesiveness was also seen in Salmonella mutants with single or different combinations of multiple mutations in genes encoding the mannose sensitive hemagglutinin (type 1 fimbriae), mannose resistant hemagglutinin and flagellum. The adhesiveness of an isogenic fur mutant was modulated by iron in a manner similar to the wild-type strain, suggesting that iron modulation of adherence is independent of the fur gene product.
국외에서 출항한 국내 입항 선박에서 검출된 한국 미분포 편승자 해충에 대한 보고 II
강태화,김상웅,홍기정,김남희,조일규,최득수,Kang, Tae Hwa,Kim, Sang Woong,Hong, Ki-Jeong,Kim, Nam Hee,Cho, Il Kyu,Choi, Deuk-Soo 한국응용곤충학회 2021 한국응용곤충학회지 Vol.60 No.3
2018년 7월 13일부터 2019년 10월 29일까지 1년 3개월 동안, 총 306개 국외 발 국내 입항 선박을 대상으로 한국 미분포 편승자 해충(not-distributed hitchhiker insect pests in Korea)에 대한 모니터링을 실시하였다. 그 결과, 총 805개체의 편승자 해충을 확보하였으며, 이를 통합 분류학적 종동정 방법(integrative identification method)을 이용하여 총 12목 78과 379종으로 동정하였다. 이 중 7목 21과 42종 67개체의 한국 미분포종이 확인되었는데, 10종이 다중검출된 것으로 나타났으며, 그 중 7종은 2018년도에 이어 2019년도에도 검출된 것으로 확인되었다. 또한, 농림축산검역본부에 관리해충으로 등재되어 있는 Erthesina fullo (Pentatomidae, Hemiptera)와 Tessaratoma papilosa (Tessaratomidae, Hemiptera)가 검출되었다. 이에 따라, 검출된 미분포종의 사전 조사 및 모니터링 방안 마련뿐만 아니라 침입종(invasive species) 내지 침입 가능종(invasive likelihood species)에 대한 위험성 평가를 위한 data sheet를 제공하였다. During 15 months from 13. Jul. 2018 to 29. Oct. 2019, we monitored on the hitchhiker insect pests from a total of 306 foreign vessels entering into Korea. As the results, a total of 805 samples of the hitchhiker insects were collected using simply collecting method by hands and identified as 379 species of 78 families under 12 orders on the basis of integrative species identification. Among them, 42 species (67 individuals) of 21 families under seven orders were detected as not-distributed species in South Korea. Of the 42 not-distributed species, seven species were multiply detected in 2018 and 2019, and two species, Erthesina fullo (Pentatomidae, Hemiptera) and Tessaratoma papilosa (Tessaratomidae, Hemiptera) were discovered as regulated insect pest. Therefore, we reported on the 42 not-distributed species in South Korea with the data sheets for the establishment of a monitoring plan on the not-distributed species and the risk assessments on invasive or invasive likelihood species.
방선균 J-144K가 생산하는 Adenosine Deaminase Inhibitor에 관한 연구
전홍기,김상웅,조영배,이인,Jun, Hong-Ki,Kim, Sam-Woong,Jo, Young-Bae,Yeeh, Yeehn 한국생명과학회 1996 생명과학회지 Vol.6 No.2
In the screening of actinomycetes culture filtrate for inhibitor of adenosine deaminase, a novel inhibitor was found in a cultured broth of strain J-144K. The optimum conditions for the adenosine deaminase inhibitor production from the isolated strain J-144K were evaluated. This strain showed the maximum yield of adenosine deaminase inhibitor when grown at pH 7.0 and 30$\circ$C for 60 hours in the medium of 1.0% dextrose, 0.5% yeast extract, 0.5% peptone and 0.1% KH$_{2}$PO$_{4}$ under the aerobic condition. Through the activated charcoal extraction, methanol fractionation, Dowex 50 H$^{+}$ X-8 ion exchange column chromatography, Dowex CI$^{-}$ X-8 ion exchange column chromatography, and Sephadex G-15 gel filtration procedures, this inhibitor was purified with three materials.