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흰쥐의 일차배양 간세포에서 Glycyrrhizin 및 Baicalin의 간 보호 활성 평가
김성화(Sung-Hwa Kim),천호준(Ho Jun Cheon),박진구(Jin-Gu Park),김영식(Yeong Shik Kim),강삼식(Sam Sik Kang),허광화(Guang Hua Xu),이승호(Seung Ho Lee),손건호(Kun Ho Son),이선미(Sun-Mee Lee) 대한약학회 2006 약학회지 Vol.50 No.6
The aim of this study was to investigate the protective effects of glycyrrhizin, active glycosides of Glycyrrhizae Radix, and baicalin, bioactive flavonoid isolated from Scutellariae Radix, on hepatocyte injury induced by carbon tetrachloride(CCl4, 10mM), tert-butyl hydroperoxide (TBH, 0.5 mM), and D-galactosamine (GalN, 30mM). Primary cultures of rat hepatocyte (18 hr cultured) were treated with CCl4, TBH, or GalN and various concentrations (0.1, 1, 10, and 100 μM) of glycyrrhizin or baicalin. Activity was accessed by determining the release of lactate dehydrogenase (LDH) and aminotransferses. CCl4 significantly increased the levels of LDH, alaine aminotransferase (ALT), and aspartate aminotransferase (AST) and these increases were prevented by baicalin concentrations of 0.1, 1, and 100μM. The increases in ALT and AST levels were reduced by glycyrrhizin concentration of 100 μM. The level of LDH was markedly increased by TBH, and this increase was reduced by both glycyrrhizin and baicalin. ALT and AST levels were increased by TBH, which were prevented by glycyrrhizin and bacalin, respectively. GalN markedly increased the levels of LDH, ALT and AST. These increases was significantly reduced by both glycyrrhizin and baicalin. These results suggest that glycyrrhizin and baicalin possess the hepatoprotective activity.
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자웅이주성 옻나무속 3수종의 자연집단에서 성비와 성간 생장량 및 공간적 분포
김삼식,이정환,정재민 ( Sam Shik Kim,Jeong Hwan Lee,Jae Min Chung ) 한국산림과학회 1998 한국산림과학회지 Vol.87 No.2
Sex ratios, and patterns of tree size and growth variation, resource allocation, spatial and age class distribution between the sexes were investigated in natural populations of the sexual trees, R. trichocarpa and R. sylvestris, and the mainly asexual, clonal tree, R. javanica of the dioecious Rhus (Anacardiaceae) distributed in Korea. Sex ratios for three species exhibited a significant degree of female bias, but among the populations, sex ratios were seen to vary quite widely. The measurement of tree size and annual increment of male trees in R. trichocarpa and R. sylvestris were significantly higher than those of female trees, but not significantly different in R. javanica. In all of the species, flowering branch number per individual and inflorescence number per branch of males outnumbered those of females. Branch number per individual, rachis(leaf) number per branch and rachis(leaf) number per inflorescence were more in females than in males. These results were considered as reproductive efforts to increase the pollen supply in males and the fruit production in females. Spatial distribution analysis in two different populations of R. trichocarpa indicated that males and females were randomly distributed in space, but seedlings were clumped around parental trees. Analysis of age class distributions between the sexual reproduction trees, R. triclaocarpa and R. sylvestris, and the asexual, clonal tree, R. javanica showed a different distribution in frequencies of males and females in each age class. These results showed that sexual and asexual reproduction tree species had almost different preference of habitats, and different sex ratio and annual growth.
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김재민,김삼식 ( Jae Min Chung,Sam Shik Kim ) 한국산림과학회 1997 한국산림과학회지 Vol.86 No.3
Morphology and anatomy of the fruit and seed of eight species of Rhus (Anacardiaceae) were investigated. The inflorescence of R. typhina was an apical and erect thysus, that of R. javanica was panicle. while that of the others were an axillary and pendulous panicle. Anatomical investigation of the pericarp showed that exocarp and mesocarp in R. javanica and R. typhina were integrated, but its in the others were disintegrated and uniform. In all these species, endocarp consisted of distinctly three lignified cell layers (outer, middle and inner endocarp) and crystal layers. The surface sculpturing of mature seed was variable and also contributed to understanding of species delimitation and relationships within the genus. Categorization by cluster analysis with 27 characters obtained from morphological and anatomical features of the fruit and seed resulted in two groups : R. trichocarpa - R. javanica group vs. R. ambigua - R. verniciflua - R. sylvestris - R. succedanea group. Taxonomic significance for the genus by these characters was supported by results of principal component analysis. The keys for the genus Rhus were provided using morphological and anatomical characters of the inflorescence, and fruit and seed.
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단풍나무속 수종의 (數種) 엽병 유관속지 (維管束枝) 변화형에 관한 연구
박광우,김삼식 ( Kwang Woo Park,Sam Shik Kim ) 한국산림과학회 1986 한국산림과학회지 Vol.73 No.1
This study was done to classify 15 species in the genus Acer through tracking of the changing shape of the vascular branch from the base part to the terminal part of petiole by the anatomical method. The basic vascular branches were 3 ea, except A. mandshuricum 3-4 ea. The vascular bundle was separated, moved and connected from the base part to the terminal part of petiol, the number of separated vascular branch was 11-32 ea., the number of connected vascular branch was 0-17 ea.., and the ratio of the separated vascular branch to the connected was 0.00-8.00. The maximum number of vascular branch in No, 39 of cross section part was observed in 12 species; A. ginnala and A. buergerianum were No. 11, A. truncatum and A. platanoides were No. 13, A. saccharium was No. 26. The number of the connected vascular branches with the main vascular branch were 0-6, while the number of the separated vascular branch were 0-7. On the ratio of separation to connection of the base vascular branch; $quot;A$quot;, $quot;B$quot; and $quot;C$quot;, the symmetrical shapes on the basis of $quot;B$quot; were A$gt;B$lt;C and A=B=C shape, the asymmetrical shape were A$lt;B$gt;C, A$lt;B$lt;C and A$gt;B$gt;C shape. The classical groups by the development of main vascular branch formation were obtained 7 groups of $quot;a$quot; through $quot;g$quot; shape. Especially, A. negundo and A. saccharinum were shown to have central vascular branches with unique changing patterns.
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