Cordyceps militaris 로부터 분리한 Ergosterol Peroxide 의 한국인 암세포주에 대한 항암작용

김하원(Ha Won Kim),김영호(Young Ho Kim),채흥복(Xing Fu Cai),남경숙(Kyong Suk Nam),이승정(Seung Jung Lee),안혜숙(Hye Suk An),정은호(Eun Ho Jeong),윤승현(Seung Hyun Yun),성수경(Su Kyong Sung),이성진(Sung Jin Lee),현진원(Jin Won Hyun) 한국균학회 2001 韓國菌學會誌 Vol.29 No.1

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Atrial Natriuretic Peptide 유사체에 의한 무마취 가토 신장기능의 변화

최병수(Byung Soo Choi),문금복(Jin Fu Wen),김화(Hua Jin),김선희(Suhn Hee Kim),강남부(Nam Poo Kang),조경우(Kyung Woo Cho) 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.1

목 적 : ANP는 이뇨와 Na^+ 배설 증가 효과 이외에도 혈관 평활근의 이완^9))과 renin 및 aldosterone 등의 분비를 억제함^10), 11)이 일찍이 알려졌다. 약물의 작용을 이해하고 생물학적 활성을 갖는 새로운 유사체를 찾는데는 화학 구조와 생물학적 활성간의 상호 관계에 대한 지식이 필요한데 ANP의 신장 효과에서는 그 보고가 일치하지 않다. 이 연구는 ANP의 화학구조와 신장 생리적 효과와의 관계를 구명하기 위한 것이다. 방 법 : ANP와 ANP의 C- 말단과 N- 말단 부분의 몇 개 아미노산이 탈락된 ANP 유사체를 ANP와 ANP의 C- 및 N- 말단의 아미노산이 탈락된 ?티드, 즉, atriopeptin Ⅲ [APⅢ, rANP- (5- 28), hANP - (7- 28)], APⅢ의 C- 말단 아미노산이 탈락된 APⅡ (rANP - (5- 27)과 API (rANP- (5- 25), 그리고 환상 구조가 풀린 hANP- (7- 28)과 hANP - (13- 28) 등 무마취 가토의 신동맥내 투여하여 나타나는 신장 기능 및 r enin 분비율의 변화를 비교 분석하였다. 결 과 : ANP 12번 아미노산이 상이한 28개 아미노산의 α- human ANP - (1- 28)(hANP )와 rat - ANP-(1- 28)(rANP) 투여에 의한 요량, 신혈류량, 사구체 여과율, Na^+ 배설량, Na^+ 분획 배설량, K^+ 배설량, Cl^- 배설량과 유리 수분 배설량의 증가 효과는 서로 유사하였다. ANP N- 말단 아미노산 4개가 탈락된 APⅡ [rANP - (5- 28)]의 요량, 신혈류 역학적 지표, Na+ 배설량, K^+ 배설량, Cl^- 배설량과 유리 수분 배설량의 증가 효과는 hANP와 rANP 보다는 약하며, N- 말단 6개 아미노산이 탈락된 hANP - (7- 28)의 요량, 신혈류 역학적 지표와 전해질 배설량 증가 효과와는 유사하였다. ANP C- 말단 아미노산 한개가 탈락된 APⅡ[rANP - (5- 27)]의 요량, 신혈류 역학적 지표와 전해질 배설량 증가 효과는 APⅢ의 신장 효과와 비교하여 더 강력하였으며, C- 말단의 아미노산 3개가 탈락된 API은 가토에서 신장 효과가 관찰되지 않았다. ANP N- 말단 부분의 아미노산이 탈락되고 disulfide 결합에 의한 환상구조가 파괴된 ANP - (13- 28)과 ANP -(18- 28)은 신장 효과가 관찰되지 않았다. hANP, rANP와 APIII는 active renin secretion rate는 감소시키고 inactive renin secretion rate는 증가시켰다. 결 론 : 이상의 연구 결과는 ANP가 이뇨 및 Na+ 배설 증가를 나타내는데는 ANP의 disulfide 결합과 C- 말단 부위 아미노산의 존재가 중요함을 암시하는 것이다. Background : Atrial cardiomyocytes synthesize, store and release atrial natriuretic peptide(ANP ) which has potent physiological effects, including natriuresis, diuresis, relaxation of vascular smooth muscle and inhibition of aldosterone and renin secretion. A family of atrial peptides are derived from a precursor proANP. However, the structure-activity relationship of several C-terminal ANPs are not yet well documented. Methods : The effects of structural difference of ANP analogs on the renal function were studied with a sensitive and reproducible bioassay using intrarenal arterial infusion in unanesthetized rabbits. Results : Rat ANP-(1-28)(rANP, 12-Ile), a-human ANP-(1-28)(hANP, 12-Met ), atriopeptin Ⅲ [APⅢ, rANP-(5-28)], atriopeptin Ⅱ[APⅡ, rANP-(5-27)], atriopeptin Ⅰ[APⅠ, rANP-(5-25)], a-human ANP-(7-28)[hANP-(7-28)], and ANP fragments (13-28) [ANP-(13-28)] and (17-28)[ANP-(17-28)] were infused into left renal artery. No significant differences were observed between rANP and hANP . Diuretic and natriuretic activities of APⅢ were significantly lower than those of rANP and hANP, but were similar to those of hANP-(7-28). Diuretic and natriuretic effects of APⅡ were similar to rANP and hANP in terms of peak responses. Duration of the effects of APⅡ were longer than those of rANP and hANP . No significant changes were observed by infusions of API, and ANP fragments, ANP-(13-28) and ANP-(17-28). rANP, hANP and APⅢ decreased active but increased inactive renin secretion. Conclusion : T hese data suggest that substitution of isoleucine to methionine at 12 position of ANP does not affect the renal effects of ANP and that disulfide bond and C-terminal segment of ANP are important for the possession of natriuretic and diuretic activities.

모체 Thyroxine 투여가 태아알코올효과를 가진 흰쥐 대뇌겉질 및 해마에서 NPY함유 신경세포의 생후 발달에 미치는 영향

김복,박상기,박영란,김종중,문정석,김주수,문영민,현영식,천관영,정윤영 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Background and Objectives: Maternal alcohol abuse is considered to be one of the most prominent cause of neurobiological malformations in the postnatal and adult life of the offspring. In this study, we investigated the effects of maternal alcohol drinking on the postnatal development of NPY-containing neuron, and, the influence of thyroxine treatment on the cerebral cortex and hippocampus of pups of alcohol abused mother. Materials and Method: Time-pregnant rats were divided into three groups. Alcohol-fed group A received 35 calories of liquid alcohol diet daily from gestation day 6; control pair-fed group B was fed a liquid diet in dextrin replaced alcohol isocalorically: alcohol + T4 group C received 35 calories liquid alcohol diet and exogenous thyroxine subcutaneously. Results: Group C showed prominent NPY immunoreactivity in the cerebral cortex compared to group A and B at P7. In group C, NPY-containing neurons were widely distributed in the all layers of cerebral cortex after P14. Besides, numerical decrease of NPY-containing neuron as age increases was not found in group C. However, the decrease of NPY-containing neuron was clearly observed in group A compared to group C after P14. In hippocampus, group Band C were appeared similar patterns after P7. Additionally, in group C, NPY immunoreactivity was prominently appeared in CA2 and CA3 at P14 as compared to group B. Conclusions: The present results showed the increase of intensity and number of NPY-containing neurons in cerebral cortex and hippocampus of pups of exogenous T₄ supplemented alcohol-exposed dams as compared to control pair-fed and alcohol-exposed pups at P7. It presumably suggest that NPY-containing neurons might be regulated by the early postnatal growth stimulatory effect of the exogenously supplemented T₄. Therefore, the increase of NPY synthesis caused by maternal administration of exogenous thyroxine may ameliorate fetal alcohol effect, one of the ill effects as a result of the dysthyroid state following maternal alcohol abuse.

출생초기 Thyroxine투여가 BDNF와 trkB함유 조롱박세포의 생후 발달 및 BDNF 단백 양에 미치는 영향

김종중,김복,정윤영 朝鮮大學校 附設 醫學硏究所 2009 The Medical Journal of Chosun University Vol.33 No.S

Background and Objectives: Thyroid hormones (TH) are essential for the development of the brain. Furthermore, TH modulate the expression of neurotrophins or their receptors. Brain-derived neurotrophic factor (BDNF) and its receptor trkB are enriched in the mammalian central nervous system. This study investigated the effect of early thyroxine (T₄) treatment on the postnatal development of BDNF- and trkB- immunoreactive (IR) Purkinje cell and BDNF protein amount in the rat cerebellum. Methods: Newborn Sprague-Dawley rats were randomly divided into two groups and treated during first 10 postnatal days, either with a daily subcutaneous injection of 0.05 ml 7.5 ㎍ L-thyroxine sodium salt in buffered 0.9% saline (group T) or with an identical volume of normal saline (group C). The morphological features of the growth and maturation were observed at 10, 15, 30 postnatal days (P10,15, 30) via immunohistochemistry. Results: The number of BDNF- and trkB-IR Purkinje cells and protein amount of BDNF were significantly higher in the group T than in the group C at P10. A similar developmental pattern of BDNF- and trkB-IR Purkinje cells was observed in the group T and C on and after P15. However, in the group T, the number of BDNF-and trkB-IR Purkinje cells and protein amount of BDNF were significantly decreased at P30 compared to P15. Conclusion: Based on the results, it can be suggested that the increase of protein amount of BDNF and BDNF-and trkB-IR Purkinje cell by early postnatal T₄ treatment is transient during early postnatal life, and these alterations are not kept until adolescence.

한국인 태아 및 사산아에서 얼굴신경에 대한 형태학적 연구

강양수,김종중,김복,정상봉,문영민,설경수,정윤영 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.3

It is critical that the accurate anatomy of the facial nerve IS known for uncomplicated successful facial surgery. Such an operation is hazardous because of the intimate relationship between the facial nerve and the expressional muscles. It is hoped that such basic information will be helpful to those who treat conditions involving the facial region. In order to define the detail of this relationship, many studies have been undertaken on dissected adult specimens. This study was based on Korean fetus and stillborn infant cadaveric dissections, and describes anatomical variations of the peripheral branches of the facial nerve that pose a potential danger in a number of surgical procedure on the face. The branching patterns were classified into six types: the frequencies of occurrence were: type I, 4.9%; type II, 24.4%; type III, 34.1%; type IV, 19.5%; type V, 12.2%; and type VI, 4.9%. Types II, III and IV together accounted for almost 80% of the specimens. In the discussion we compare the results of the present study with those of others and try to clarify the anatomical characteristics of the facial nerve in infants, which demand that special attention be paid to the avoidance of injuries during facial surgery.

편측 안면경련 환자에 대한 3-dimensional Magnetic Resonance Angiography와 Constructive Interference in Steady State(CISS)의 유용성

박치후,이봉암,박종태,임영진,김태성,임언,김국기 대한신경외과학회 1999 Journal of Korean neurosurgical society Vol.28 No.3

양성 낭종이 동반된 편측 안면 경련

박치후,이봉암,박종태,임영진,김태성,임언,김국기 대한신경외과학회 1999 Journal of Korean neurosurgical society Vol.28 No.3