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김도영,Hyun Chul Kim,Sun Young Kim,이영하 한국미생물학회 2005 The journal of microbiology Vol.43 No.3
A bacterial strain M4-7 capable of degrading various polyesters, such as poly(ε-caprolactone), poly(3-hydroxybutyrate-co-3-hydroxyvalerate),poly(3hydroxyoctanoate), and poly(3-hydroxy-5-phenylvalerate),was isolated from a marine environment and identified as Pseudomonas alcaligenes. The relative molecular mass of a purified extracellular medium-chain-length poly(3-hydroxyalkanoate) (MCL-PHA)depolymerase (PhaZPalM4-7) from P. alcaligenes M4-7 was 28.0 kDa, as determined by SDS-PAGE. The PhaZPalM4-7 was most active in 50 mM glycine-NaOH buffer (pH 9.0) at 35oC. It was insensitive to dithiothreitol, sodium azide, and iodoacetamide, but susceptible to p-hydroxymercuribenzoic acid,bromosuccinimide, acetic anhydride, EDTA, diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride,Tween 80, and Triton X-100. In this study, the genes encoding MCL-PHA depolymerase were cloned,sequenced, and characterized from a soil bacterium, P. alcaligenes LB19 (Kim et al., 2002, Biomacromolecules 3, 291-296) as well as P. alcaligenes M4-7. The structural gene (phaZPalLB19) of MCL-PHA depolymerase of P. alcaligenes LB19 consisted of an 837 bp open reading frame (ORF) encoding a protein of 278 amino acids with a deduced Mr of 30,188 Da. However, the MCL-PHA depolymerase gene (phaZPalM4-7) of P. alcaligenes M4-7 was composed of an 834 bp ORF encoding a protein of 277 amino acids with a deduced Mr of 30,323 Da. Amino acid sequence analyses showed that, in the two different polypeptides, a substrate-binding domain and a catalytic domain are located in the N-terminus and in the C-erminus,respectively. The PhaZPalLB19 and the PhaZPalM4-7 commonly share the lipase box, GISSG,in their catalytic domains, and utilize 111Asn and 110Ser residues, respectively, as oxyanions that play an important role in transition-state stabilization of hydrolytic reactions. A bacterial strain M4-7 capable of degrading various polyesters, such as poly (ε-caprolactone), poly(3-hydroxybutyrate-co-3-hydroxyvalerate),poly(3hydroxyoctanoate), and poly(3-hydroxy-5-phenylvalerate), was isolated from a marine environment and identified as Pseudomonas alcaligenes. The relative molecular mass of a purified extracellular medium-chain-length poly(3-hydroxyalkanoate) (MCL-PHA)depolymerase (PhaZPalM4-7) from P. alcaligenes M4-7 was 28.0 kDa, as determined by SDS-PAGE. The PhaZPalM4-7 was most active in 50 mM glycine-NaOH buffer (pH 9.0) at 35oC. It was insensitive to dithiothreitol, sodium azide, and iodoacetamide, but susceptible to p-hydroxymercuribenzoic acid,bromosuccinimide, acetic anhydride, EDTA, diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride,Tween 80, and Triton X-100. In this study, the genes encoding MCL-PHA depolymerase were cloned,sequenced, and characterized from a soil bacterium, P. alcaligenes LB19 (Kim et al., 2002, Biomacromolecules 3, 291-296) as well as P. alcaligenes M4-7. The structural gene (phaZPalLB19) of MCL-PHA depolymerase of P. alcaligenes LB19 consisted of an 837 bp open reading frame (ORF) encoding a protein of 278 amino acids with a deduced Mr of 30,188 Da. However, the MCL-PHA depolymerase gene (phaZPalM4-7) of P. alcaligenes M4-7 was composed of an 834 bp ORF encoding a protein of 277 amino acids with a deduced Mr of 30,323 Da. Amino acid sequence analyses showed that, in the two different polypeptides, a substrate-binding domain and a catalytic domain are located in the N-terminus and in the C-erminus,respectively. The PhaZPalLB19 and the PhaZPalM4-7 commonly share the lipase box, GISSG, in their catalytic domains, and utilize 111Asn and 110Ser residues, respectively, as oxyanions that play an important role in transition-state stabilization of hydrolytic reactions.
광검출기 응용을 위하여 스퍼터된 미세결정 SiGe 박막성장 연구
김도영,김선조,김형준,한상윤,송준호,Kim, Do-Young,Kim, Sun-Jo,Kim, Hyung-Jun,Han, Sang-Youn,Song, Jun-Ho 한국전기전자재료학회 2012 전기전자재료학회논문지 Vol.25 No.6
For the application of photo-detector as active layer, we have studied how to deposit SiGe thin film using an independent Si target and Ge target, respectively. Both targets were synthesized by purity of 99.999%. Plasma generators were generated by radio frequency (rf, 13.56 MHz) and direct current (dc) power. When Ge and Si targets were sputtered by dc and rf power, respectively, we could observe the growth of highly crystalline Ge thin film at the temperature of $400^{\circ}C$ from the result of raman spectroscopy and X-ray diffraction method. However, SiGe thin film did not deposit above method. Inversely, we changed target position like that Ge and Si targets were sputtered by rf and dc power, respectively. Although Ge crystalline growth without Si target sputtering deteriorated considerably, the growth of SiGe thin film was observed with increase of Si dc power. SiGe thin film was evaluated as microcrystalline phase which included (111) and (220) plane by X-ray diffraction method.
이산분포 HMM을 이용한 음성인식에서의 코드워드 Tying 알고리즘
김도영,김남수,은종관,Kim, Do-Yeong,Kim, Nam-Soo,Un, Chong-Kwan 한국음향학회 1994 韓國音響學會誌 Vol.13 No.3
본 논문에서는 수형구조 분류기를 이 용한 코드워드 tying 알고리즘을 제안한다. 코드워드와 상태간의 통계적 특성을 이용한 일종의 soft decision 방식이라고도 볼 수 있는 제안된 알고리즘은 빠른 트리 구성과 유일한 최적의 해를 제공하는 특징이 있다. 또한, 이산분포 hidden Markov model(HMM)을 이용한 인식 시스템에 쉽게 적용이 가능하다는 장점을 가진다. 제안된 알고리즘의 성능 평가를 위한 화자독립 격리단어 인식실험에서 코드북 크기가 256과 512일 경우에 대해 각각 $6\%$, $9\%$의 오차를 감소시켰으며, HMM 파라미터도 $20\%$ 정도 줄임을 확인하였다. In this Paper, we propose a new codeword tying algorithm based on a tree structured classfier. The proposed algorithm which can be viewed as a kind of soft decision using statistical properties between codewords and states has an advantage of fast construction, and guarantees a unique optimal solution. Also, it can easily be applied to any speech recognition system based on discrete hidden Markov model (HMM). Experimental results on speaker-independent isolated word recognition show error reduction of $6\%$ for the codebook of size 256 and $9\%$ for 512 size and also HMM parameter reduction of about $20\%$.
프로토타이핑 기법에 의한 키네틱 외피의 설계: 디지털-아날로그 모델의 상호작용과 BIM의 역할을 중심으로
김도영,김성아,Kim, Do-Young,Kim, Sung-Ah 한국BIM학회 2015 KIBIM Magazine Vol.5 No.1
The kinetic facade system is an interactive building envelope which is adaptive to environmental condition by transforming the behaviour of its components. The design process of kinetic facade system calls for a novel approach. It needs to support designers to adopt technologies from multidisciplinary fields such as physical computing and robotics. In this paper, prototyping method is introduced as a useful technique for implementing kinetic facade systems. In order to incorporate prototyping method into architectural design process, two aspects are investigated in digital design studio: (1) The interactions between digital and analogue environments (2) The role of traditional design tools. Furthermore, the role of BIM is investigated by analyzing two aspects.
Antiglide 금속판을 이용한 족관절의 외과 골절의 치료
김도영,신주호,조원호,황현철,Kim, Do-Young,Shin, Joo-Ho,Cho, Won-Ho,Hwang, Hyun-Chull 대한족부족관절학회 2001 대한족부족관절학회지 Vol.5 No.1
Most of Danis-Weber type B fracture has an oblique fracture plane proximal posteriorly to distal anteriorly. In these cases, the lateral plate has become widely accepted. However some limitation to the use of the lateral plate exist. The plate must be bent accurately and the screws in the distal fragment must be unicortical to avoid penetration into the joint, predisposing it to poor fixation in the osteoporotic bone. In 1982, Brunner and Weber introduced the antiglide plate for fixation of the shaft oblique fracture of the distal fibula, but it has not widely used. The author reviewed 21 cases treated by antiglide plate fixation from March, 1995 to March, 1999 which could be follow-up more than 1 year. We analysed the result radiographically and clinically using Ankle-Hindfoot scale(100 % total) of the American Orthopedic Foot and Ankle Society. The results obrained were as follows: 1. All fracture were united at average 8 weeks clinically and radiographically. 2. According to the Ankle- Hindfoot scale, 8 cases were above 90 points, 11 cases were beet ween 80 and 89 points and two cases were below 80 points. 3. One case had an injury to intermediate dorsal cutaneous nerve.
연속분포 HMM을 이용한 한국어 연속 음성 인식 시스템 개발
김도영,박용규,권오욱,은종관,박성현,Kim, Do-Yeong,Park, Yong-Kyu,Kwon, Oh-Wook,Un, Chong-Kwan,Park, Seong-Hyun 한국음향학회 1994 韓國音響學會誌 Vol.13 No.1
In this paper, we report on the development of a speaker independent continuous speech recognition system using continuous hidden Markov models. The continuous hidden Markov model consists of mean and covariance matrices and directly models speech signal parameters, therefore does not have quantization error. Filter bank coefficients with their 1st and 2nd-order derivatives are used as feature vectors to represent the dynamic features of speech signal. We use the segmental K-means algorithm as a training algorithm and triphone as a recognition unit to alleviate performance degradation due to coarticulation problems critical in continuous speech recognition. Also, we use the one-pass search algorithm that Is advantageous in speeding-up the recognition time. Experimental results show that the system attains the recognition accuracy of $83\%$ without grammar and $94\%$ with finite state networks in speaker-indepdent speech recognition. 본 논문에서는 연속분포 hidden Markov모델을 이용한 화자독립 연속 음성 인식 시스템에 관해 기술한다. 연속분포 모델은 평균과 분산 벡터로 구성되며 음성신호를 직접 모델링하여 양자화 왜곡이 없어진다. 특징벡터는 filter bank 계수 및 그 1, 2차 미분계수를 사용하여 음성신호의 동적 특성을 반영하였다. Segmental K-means 알고리즘을 이용하여 학습하였으며, 연속어 인식에서 가장 문제가 되는 조음화 현상으로 인한 인식률 저하를 막기 위해 앞뒤의 음소를 고려해주는 triphone을 인식단위로 사용하였다. Search 알고리즘으로는 시간 면에서 효율이 좋은 one-pass search 알고리즘을 사용하였다 성능 평가를 위한 회자 독립인식 실험에서 문법이 없을 경우 $83\%$, finite state network을 적용한 경우에는 $94\%$의 인식률을 나타내었다.
PSTN에서의 화상통신 기술분석 및 국내표준 방식(안) 검토
김도영,윤용섭,이종수,Kim, Do-Yeong,Yun, Yong-Seop,Lee, Jong-Su 한국전자통신연구원 1989 전자통신동향분석 Vol.4 No.2
이 논문은 PSTN에서 운용되는 화상전화기의 국내표준방식에 관한 연구결과로서, 화상통신 기술 및 선행 사례들을 분석.기술하고 있다. 특히 진폭-위상 변조 방식을 이용한 화상통신 방식 (일본 TTC규격)과 모뎀을 이용한 화상통신 방식을 비교.분석하였으며, 이들을 토대로 적절한 PSTN용 화상전화기 국내표준 방식(안)을 제시하였다.
태양전지응용을 위하여 MOCVD 방법으로 성장된 ZnO 박막의 기판온도에 따른 표면특성
김도영,강혜민,김형준,Kim, Do-Young,Kang, Hye-Min,Kim, Hyung-Jun 한국진공학회 2010 Applied Science and Convergence Technology Vol.19 No.3
우리는 MOCVD (metal organic chemical vapor deposition)법을 이용하여 DEZ (Diethylzinc)을 운송하는 Ar 유속과 reactant (반응물질)의 종류에 따른 ZnO 박막 증착을 연구하였다. Bubbler 시스템을 통하여 주입되는 Ar 유속에 의해 Zn 소스인 DEZ의 양이 조절된다. 산소 기체와 수증기는 산화를 위해 반응물질로 사용된다. 본 연구로부터 표면의 거칠기(surface roughness)는 반응물질의 종류와 DEZ Ar 유속에 관계되며 박막의 두께에 의존한다는 것을 알 수 있었다. 그러나 기판 온도는 산소를 반응물로 하는 상태에서는 표면 거칠기에 영향을 주지 못함을 알 수 있었다. 우리는 ZnO 박막이 90 sccm (Standard Cubic Centimeter per Minute)의 DEZ Ar 유속, 8 Pa의 수증기압, 그리고 $140^{\circ}C$의 기판 온도에서 39.16 mm의 가장 높은 거칠기를 가진다는 것을 확인할 수 있었다. 본 논문은 태양전지의 광 흡수층으로 사용가능한 ZnO 박막을 연구하였다. We report on the deposition of ZnO films using a metal organic chemical vapor deposition (MOCVD) as a function of pushing pressure and kind of reactant such as oxygen gas and water A diethylzinc (DEZ) is supplied and controlled by Ar pushing pressure through bubbling system. Oxygen gas and water are used as reactant in order to form oxidation. We knew that the surface roughness is related in the process conditions such as reactant kind and DEZ flow rate. A substrate temperature has little role of surface roughness with $O_2$ reactant. However, $H_2O$ reactant makes it to increase over the 20 times. We could get the maximum roughness of 39.16 nm at the 90 sccm of DEZ Ar flow rate, the 8 Pa of $H_2O$ vapor pressure, and the $140^{\circ}C$ of substrate temperature. In this paper, we investigated the ZnO films for the application to the light absorption layer of solar cell layer.
실무 3D 스캐닝 및 BIM 활용을 위한 발주자 - 실무자 간 협업프로세스 모델
김도영,Kim, Do-Young 한국BIM학회 2021 KIBIM Magazine Vol.11 No.2
In construction sites, policies are changing considering the convergence of 3D scanning and BIM. In order to respond to this, it is urgent to develop guidelines for systematic collaboration methods that take into account the perspectives of practitioners. By participating in the delivery process using 3D scanning technology, tasks such as ordering, field scanning are defined in terms of mutual communications. Also, the collaboration process is about communications between off-site and on-site, such as feed-back using data and documents. In the future, we will propose guidelines based on such collaborative process models.