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김길하,송철,장부영,박노중,조광연,Kim, Gil-Hah,Song, Cheol,Chang, Bu-Young,Park, No-Joong,Cho, Kwang-Yun 한국응용곤충학회 1995 한국응용곤충학회지 Vol.34 No.1
점박이응애(Tetranychus urticae Koch)의 감수성계통(S)과 dicofol 저항성계통(R)을 공시하여 dicofol저항성의 안정성을 조사하였다. Dicofol 저항성계통(저항성비 23.0)의 약제도태없는 조건하에서 사육된 암컷성충의 dicofol에 대한 저항성 수준은 1년이 경과된 후에도 변화가 없었다. 그러나 1년 이후부터는 감소하기 시작하여 2년째에는 저항성비가 15.8배, 3년째에는 10.7배로 감수성쪽으로 기울었다. 점박이응애의 dicofol 저항성계통과 감수서계통의 상호교배 $(R_{female}\timesS_{male},R_{male}\times S_{female})$에서 얻어진 세대별 약제 감수성도 세대가 경과됨에 따라 감수성쪽으로 기울었다. Stability of resistance of the two-spotted spider mite (Tetranychus urticae) to dicofol was investigated. Resistance level (23.0-fold) of adult females to dicofol was kept up to 1 year without selection by dicofol, but was decreased to 15.8-, 10.7-fold after 2 and 3 years, respectively. Susceptibility of adult females obtained from reciprocal crosses $(R_{female}\timesS_{male},R_{male}\times S_{female})$ of the R and S stains to dicofol was declined by the success of generation.
Dicofol 저항성 점박이응애의 저항성 유전과 교차저항성
김길하,송철,박노중,조광연,Kim, Gil-Hah,Song, Cheol,Park, No-Joong,Cho, Kwang-Yun 한국응용곤충학회 1994 한국응용곤충학회지 Vol.33 No.4
실내에서 사육한 점박이응애(Tetranychus urticae Koch)의 감수성계통(S)을 유기염소계 살비제안인 dico-fol을 공시하여 인위적으로 도태선발하여 저항성 발달과 저항성 유전을 조사하고, 교차저항성 유무를 검토하였다. Dicofol을 150회 처리하여 얻은 선발계통($R_d$</TEX)은 감수성계통에 비하여 $LC_{50}$값이 27.5배증가하였다. 감수성계통과 저항성계통의 상호교배에서 얻어진 $F_1$ (R♀, XS♂, R♂, XS♀)은 농도와 사망률 관계에서 감수성 차이가 있었다. 또 $F_1$ (R♀, XS♂, R♂, XS♀)의 우성도는 암컷성충에서 각각 -0.29, -0.72, 수컷성충에서 각각 -0.42, -0.93로 나타났다. 따라서 dicofol 저항성 유전자는 불완전열성에 의해 지배되는 것으로 판단되었다. $R_d$ 계통 은 아미트라즈계인 amitraz에 대해서 높은 교차저항성을 나타내었고, 또한 pyethroid 계 살비제인 acrinathrin, bifenthrin에 대해서도 교차저항성을 나타내었다. Abamectin, chlofenson, clofente-zine, cyhexatin, fenbutation oxide, fenpyroximate, hexythiazox, monocrotophos, propargite, tetradifon에 대해서는 비교차저항성을 나타내었다. 한편 유기주석계인 azocyclotin과 fenbutatin oxide에 대해서느 역상관교차저항성을 나타내었다. Dicofol susceptible two-spotted spider mite(S) which was selected by 150 times of dicofol treatment showed 27 5-fold increase in resistance as compared to S strain. Inheritance of resistance and crossresistance in selected strain($R_d$) were investigated. There were differences oi susceptibility in the dicofol concentmtion-moltaliity relationships between $F_1$ progenies (R♀, XS♂, R♂, XS♀) obtained from reciprocal cross with the S strain and $R_d$, strain. Degree of dominance of the <TEx>$F_1$ progenies (R♀, XS♂, R♂, XS♀) were -0.29 and -0.72 in adult females and -0.42 and -0.93 in adult males, respectively. These results suggest that inheritance of dicofol resistance is controlled by an incomplete recessive. $R_d$ strain exhibited a high level of cross resistance to amitraz, and also showed cross resistance to pyrethrotd acaricides, acrinathrin and bifenthnn. $R_d$ strain showed non-cross reslstancz to abamectin, chlolfenson, clofentezine, cyhexatm, fenbutatin oxlde, fenpyroximate, hexythiazox, monocrotophos, propargite and tetradifon, but negatively correlated cross-resistance to azocyclotin and fenbutatin oxide belonging to tin acaricides.
충북지역 노지포도원에서 열점박이별잎벌레 (Oides decempunctatus)의 발생생태 및 발육특성
김선국 ( Sun Kook Kim ),윤창만 ( Changmann Yoon ),이석호 ( Seok Ho Lee ),장후봉 ( Who Bong Chang ),김이기 ( Yee Gi Kim ),김길하 ( Gil Hah Kim ),김형환 ( Hyeong Hwan Kim ) 한국토양동물학회 2015 한국토양동물학회지 Vol.19 No.1
This study was conducted to investigate the seasonal occurrence of Oides decempunctatus (Coleoptera: Chrysomelidae) and it`s damage on grape according to the variety. In Chungbuk province, occurrence of O. decempunctatus was from 20 to 38% at open field vineyard during 2011 to 2012. O. decempunctatus showed the highest peak in mid-late June (larva) and mid-late August (adult). Overwintered eggs of O. decempunctatus were hatched from late May to early June. Larval period was from late May to mid July and pupal period was taken one month during July under the ground soil. Adults appeared in mid July and it began ovipositing on the ground near the crown until mid October. Among 39 varieties, Seyve villard 5247 was damaged more than 50% by this pest, and followed 6 varieties including Baco 22A were damaged within 20~50%. Whereas, Neo Muscat, MBA and Delaware were damaged less than 5%. Developmental period of O. decempunctatus from egg to emergence was taken times as 86.0 days at 20℃ and 40.7 days at 30℃. The hatching rate was the highest at 25℃ showing 79.9%, wheres it was decreased to 39.8% at 30℃. Each developmental zero point (15, 20, 25, 30±1℃, 60±5% RH) was recorded as 9.9, 11.5, 9.1, 9.3℃ and effective accumulated temperature was investigated 166.6, 290.9, 404.2, 913.2 Degree Day (DD).