와송 다당체 및 올리고당류의 생리 활성

김기훈,김은영,김예운,백금옥,김한복,이동석,Kim Ki Hoon,Kim Eun Young,Kim Yea Oon,Baek Geum Ok,Kim Han Bok,Lee Dong Seok 한국미생물학회 2004 미생물학회지 Vol.40 No.4

와송으로부터 고온 고압의 열수 추출로 다당체 조추출물(OJP1)을 얻어 디스크 확산법, fluorescein diacetate(PDA)법 및 액체 배지 희석법을 통하여 세균과 진균에 대한 항균 활성을 조사하였다. 또한 Sephadex G-50을 통하여 다당체(FI)와 올리고당류(FII)를 분리하고, 이들의 항암 활성을 조사하였다. FI과 FII의 분자량은 각각 30$\~$50 kDa과 1$\~$3 kDa으로 추정되었다. OJP1의 항균 효과는 디스크 확산법에서 Candida albicans가 $20\pm4.9\;mm$의 억제대로 가장 높았으며, Salmonella typhimurium과 Staphylococcus aureus도 각각 $18\pm2.0\;mm$ 와 $17\pm1.0\;mm$의 억제대로 비교적 높게 나타났다. 또한 Escherichia coli와 Pseudomonas aeruginosa에 대해서도 양성 대조군인 프로폴리스보다 높게 나타났으며, FDA법, 액체배지 희석법에서도 비슷한 양상을 나타냈다. OJP1과 Fl, FII의 인체 암세포주와 Sarcoma 180 세포주에 대한 항암 활성을 측정해 본 결과, 이들은 모두 MTT assay와 형태 변화 관찰에서 강력한 암세포주 증식 억제 효과를 나타내었는데, 특히 폐암 세포주인 A549 세포와 자궁 경부암 세포주인 HeLa 세포, 위암 세포주인 AGS에서 현저한 효과를 보였다. 더 나아가 DNA 분절화를 통해 apoptosis 유발 여부를 확인해 본 결과, 대조군에 비해서 이들 물질을 처리한 실험군에서 apoptosis발생을 뜻하는 DNA 분절화 현상이 뚜fut하게 나타났다. 요컨대, OJP1과 Fl, FII는 광범위한 병원성 균주에 대하여 효과적인 항균 활성을 보였으며, 각종 암세포주에 대하여 현저한 항암 활성을 나타내는 것으로 확인되었다. Polysaccharides were prepared from Orostachys japonicus by extration with hot steam water (OJPl). The OIPl fraction was further purified by Sephadex G-50 gel filtration chromatography to produce FI (polysaccharides) and FII (oligosaccharides) fraction. The average molecular masses o fFI and FII fraction were determined to be 3050 kDa and 13 kDa, respectively. The antimicrobial activity of OIPl was tested against 8 strains of bacteria and one strain of yeast by the disc diffusion method, fluorescein diacetate (FDA) method and broth dilution method. The OIPl exhibited a very strong growth inhibition to Candida albicans. The OIPl remarkably sup­pressed the growth of Salmonella typhimurium and Staphylococcus aureus. The OIPl showed higher growth inhibition to Escherichia coli and Pseudomonas aeruginosa than propolis, positive control. When the anticancer activity of the OIPl, FI or FII was examined against human cancer cell lines and the Sarcoma 180 cells, these widely suppressed the proliferation of cell lines in the MTT assay and morphology study. Especially, they remarkably inhibited the growth of A549, HeLa and AGS cells. Also treatment of cancer cells with OJPl, FI or FII induced apoptotic cell death characterized by DNA fragmentation. The OJPl, FI or FII exhibiting various biological activities such as antimicrobial activity and anticancer activity is expected to be developed as new biohealth products.

Short-Long R-R Sequence Induced Implantable Cardioverter Defibrillator Shock by Dislodged Lead Even Having Normal Interrogation Parameter

김기훈,Sang-Hoon Seol,Seung Hwan Kim,Dong-Kie Kim,Doo-IL Kim 대한부정맥학회 2018 International Journal of Arrhythmia Vol.19 No.1

Inappropriate shocks from an implantable cardioverter defibrillator (ICD) can cause potentially dangerous ventricular arrhythmias and impaired quality of life. We describe a case in which a dislodged lead caused inappropriate ICD shocks through simultaneous sensing of atrial and ventricular signals. Interestingly, repeated short-long R-R sequences were recorded, but ICD interrogation parameters were usually unchanged.

바이러스성 뇌수막염(Viral Encephalitis) 후유기(後遺期) 환아(患兒) 1례(例)에 대한 증례(症例)

김기훈,신동길,김덕곤,Kim, Ki-Hoon,Shin, Dong-Gil,Kim, Deog-Kon 대한한방소아과학회 2002 대한한방소아과학회지 Vol.16 No.1

Viral Encephalitis are a serious meningeal infection occurred by various chemical substance. Chief symptoms are headache. hyperesthesia in child and fretting. irritation in infant. Adolescence complain of a retrobulbar pain, fever, vomiturition, photophobia. Fever can cause choreic movement, mental disorder and seizure. Most of patients have complete recovery, but if there are brain lesions, it is bad prognosis. Occasionally patients complain of continuous headache, mental disorder, ataxia and marasmus. We report on a 4-year-old male patient with viral encephalitis. It has been 40 days since disease. He had disorientation mentality, dysphagia and fever.

제11차 ASTAP(APT Standardization Program)

김기훈,박종봉,Kim, Gi-Hun,Park, Jong-Bong 한국정보통신기술협회 2006 TTA저널 Vol.106 No.-

제대군인 취업에 관한 연구 - 민간기업 취업을 중심으로 -

김기훈,Kim, Ki-Hoon 대전대학교 군사연구원 2011 군사학연구 Vol.9 No.-

The Republic of Korea, ranked as the 5th military power according to the size of its ground troops, discharges more than 200,000 manpower to the society annually; more than 13,000 of them are officers. The re-employment rate of discharged military personnels, who experience early retirement of 5 to 15 years compared to general public officers, is very low, only amounting to 53.2%. On the other hand, major developed countries exhibit great national scale of effort to take responsibility in helping the settlement of the soldiers who have dedicated their career for their countries. The re-employment rate amounts to 94%. Fully acknowledging such disparity, this paper examines various policies on discharged members support of developed countries, and aims to suggest developmental model on employment support designed for discharged members in the Republic of Korea.

'왕의 섬유'캐시미어

김기훈,Kim, Gi-Hun 기술표준원 2006 기술표준 Vol.49 No.-

Hagwilget Bridge

김기훈,Kim, Gi-Hun 한국건설관리학회 2013 한국건설관리학회 논문집 Vol.14 No.6

유관기관칼럼 - 양모/캐시미어 감별에 있어서 미확인 모의 종류와 그 데이터 관리

김기훈,Kim, Gi-Hun 기술표준원 2009 기술표준 Vol.88 No.-

콘크리트충전 각형강관구조의 다이아프램 개구부 형상에 따른 기둥-보 접합부 구조적 거동

김기훈,이명재,Kim, Ki Hoon,Lee, Myung Jae 한국강구조학회 2015 韓國鋼構造學會 論文集 Vol.27 No.3

CFT 구조는 각형이나 원형강관에 콘크리트가 충전된 구조로서, 강관은 콘크리트를 구속하고 콘크리트는 강관의 좌굴을 방지함으로써 부재의 강성 및 강도를 증가시킬 수 있다. 이에 본 연구는 관통 다이아프램 충전개구부 형상이 각형CFT 기둥-보 접합부의 내력에 미치는 영향을 실험을 통해 확인한 후, 유한요소해석을 수행하여 실험 결과와 비교 검증했다. 이로써 각형CFT 기둥-보 접합부의 내력은 관통 다이아프램의 개구부 면적이 같을 경우 그 형상에 크게 영향을 받지 않으며, 관통 다이아프램과 보 플랜지의 접합부분 형상 및 치수가 동일하면, 보에 발생한 휨응력이 다이아프램을 통해 기둥으로 전달되고 있음을 확인하였다. The steel tube of Concrete-Filled Tube(CFT) confines the concrete and the concrete restrains the buckling of the tube, The objective of this study is to investigate the influences of the opening shape of the through diaphragm in case of the rectangular CFT column-to-beam connection through the structural experiment. The experiment results are compared with analysis results obtained by using the FEM program. These results are shown that strength of the rectangular CFT column-to-beam connection have similar structural performance regardless of the opening shape if opening areas of the through diaphragm are same. Also in case the connection area/shape of the through diaphragm and the flange of H-beam are similar, it was ascertained that the bending stress occurred at the beam can be transferred to the column through the diaphragm.

특발성 진성 성조숙증으로 진단된 소아의 원인 및 임상적 분석

김기훈,신동길,김대현,Kim, Ki-Hoon,Shin, Dong-Gil,Kim, Dae-Hyun 대한한방소아과학회 2011 대한한방소아과학회지 Vol.25 No.2

Objectives: The purpose of this study is to estimate genetic and environmental factors, which can affect Idiopathic true Precocious puberty, and to evaluate the clinical and endocrinologic characteristics. Methods: Retrospective and Comparative analysis of 76 children (72 girls and 4 boys) has been diagnosed with idiopathic true precocious puberty, and treated with GnRHa from December 2008 to July 2011. Results: 1. The Average chronological age (CA. yr) of children diagnosed with idiopathic true precocious puberty was $8.40{\pm}0.81$ (girls), $9.93{\pm}0.12$ (boys). 2. The Average height & weight percentile (%ile) of the girls diagnosed with idiopathic true precocious puberty was $67.38{\pm}22.04$, $67.69{\pm}23.20$. 3. The girls' mothers have diagnosed with idiopathic true precocious puberty, and they were shorter than the average. This shows that mother's small height and idiopathic true precocious puberty are closely related to each other. 4. BMI percentile (%ile) of girls diagnosed with idiopathic true precocious puberty was $63.26{\pm}24.86$. 23.6% of children were diagnosed with overweight or obesity. This result shows that obesity and idiopathic true precocious puberty are proportionally related. 5. Birth weights (kg) of the children diagnosed with idiopathic true precocious puberty were $3.16{\pm}0.43$ (girls), $3.15{\pm}0.38$ (boys). 8.3% of children were diagnosed with Intrauterine growth retardation. 6. The Average bone ages (BA. yr) of the children diagnosed with idiopathic true precocious puberty were $10.51{\pm}0.99$ (girls), $12.10{\pm}0.97$ (boys). The Average BA-CA was $2.11{\pm}0.81$ (girls), $2.00{\pm}0.87$ (boys). 7. The Average predicted adults' height (PAH. cm) of the children diagnosed with idiopathic true precocious puberty was $151.61{\pm}4.00$ (girls), $163.50{\pm}2.15$ (boys). The Average MPH-PAH was $6.84{\pm}4.91$ (girls), $6.00{\pm}5.35$ (boys). 8. 23.6% of the children treated with GnRHa were co-treated with Growth Hormone. Conclusions: Estimated factors which cause Idiopathic true precocious puberty are mother's small height, obesity, and Intrauterine growth retardation. However, the studies of Oriental Medicine for Idiopathic true precocious puberty were lacking. Further clinical and experimental researches are needed.