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소화관 기계적 폐쇄에 따른 Interstitial Cells of Cajal (ICC) 및 위장관운동의 변화
전제열(Jae-Yeoul Jun),정춘해(Choon-Hae Chung),유호진(Ho-Jin You),김경희(Kyung-Hee Kim),김장만(Jang-Man Kim),김기훈(Kee-Hune Kim),박도영(Do-Young Park),장인엽(In-Youb Chang) 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.5
Interstitial cells of Cajal (ICC)는 내장 민무늬근세포와 소화관 신경종말들과 밀접한 해부학적 연관성을 가지고 있으며, ICC가 소화관 연동운동에 있어서 소화관 연동운동의 향도잡이 역할을 할 뿐만 아니라, 소화관신경으로부터 민무늬근세포로 전해지는 신경전달을 매개, 조절한다고 알려져 있다. 최근 여러 소화관운동장애 질환에서 ICC의 감소 및 소실이 병인과 연관성이 있다는 주장들이 제기되는 실정이다. 이에 따라 저자들은 수술적으로 생쥐 작은창자를 부분폐쇄를 시킨 다음, 작은창자 근육층의 비대증을 유도한 후 소화관 운동의 변화 및 ICC의 형태학적 변화를 밝혀내고자 ICC에 대한 면역형광염색과 전기생리학적 실험을 병행하여 실시하였다. 작은창자를 부분폐쇄시키고 2주 후, 폐쇄부위보다 입쪽의 작은창자부위에서 육안적으로 창자의 직경이 증가하고, 광학현미경적으로 근육층의 비대가 나타나는 것을 관찰할 수 있었다. ICC에 대한 면역형광염색(c-kit staining) 결과, ICC 그물망이 폐쇄부위보다 입쪽으로 25 mm 정도까지 소실 또는 뚜렷하게 감소되는 것을 관찰할 수 있었으며, 이러한 형태학적 결과와 병행하여 전기생리학적으로도 서파의 소실 또는 감소가 나타남을 관찰할 수 있었다. 그러나 폐쇄부위보다 항문쪽에서는 ICC와 서파의 이상소견은 관찰되지 않았다. 본 실험 결과 소화관의 폐쇄에 따라 입쪽부위 ICC의 소실과 서파의 소실을 관찰할 수 있었다. 따라서 앞으로 ICC 구조를 변화하게 하는 분자생물학적 그리고 유전학적 신호체계에 대해 면밀한 분석을 할 경우 소화관운동장애의 발생원인 및 치료에 계기를 마련할 수 있을 것으로 사료된다. Interstitial cells of Cajal (ICC) are the pacemakers in gastrointestinal slow wave, and also transduce signal inputs from the enteric nervous system to smooth muscle. The abnormal motility corresponded to a lack or decreasing of ICC and a disruption of electrical slow waves. So we developed partial obstruction model in murine small intestine and investigated changes in the ICC networks and electrical activity in the obstructed bowel using c-kit immunohistochemistry and intracelluar electrophysiological techniques. Two weeks following the onset of a partial obstruction, the small intestine increased in diameter and muscular hypertrophy was developed oral to the obstruction site. ICC were absent or only weak at 1~25 mm oral to the occlusion site, and this disruption was accompanied by the loss of electrical slow wave. ICC networks and slow waves were normal appearance aboral to the clip. In conclusion, The present results showed that partial intestinal obstruction induced the loss of ICC networks and slow waves. These result will provide a valuable aid for understanding pathogenesis of intestinal motility disorder, and this model may be an important tool for evaluating genetic or molecular factor for the therapeutic opportunities of motility disorder in human.
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안구적출에 따른 위둔덕의 Calbindin D-28k과 c-fos의 변화에 관한 연구
김명,김기훈,김주영,하현철,안명수,김장민,조향훈,정명섭,장인엽 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2
Changes of Calbindin D-28k- and c-fos-immunoreactivities in the superior collicuclus after Eye Enucleation. Objective and methods: Calcium-binding proteins (CBPs) play an important role in the protection, differentiation and reorganization of the central nervous system. The effects of neonatal retinal deafferentation on a CBPs, calbindin D-28k were examined immunohistochemically in the superficial layer of the rat superior colliculus. Also early gene familly c-fos was examined to evaluate the neuronal characteristics in the superior colliculus after monocular enucleation. Results: On the experimental side of superior colliculus, the number of calbindin D-28k-immunoreactive (IR) cells was reduced (77.4% compared to control), but not fibers. Appearance of c-fos-like immunoreactivity was represented much more in the ipsilateral superior colliculus than contralateral side within 24h after eye enucleation. Conclusion: These results suggest that the changes of retinotectal projection may alter the expressional pattern of calbindin D-28k and c-fos expression.
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생쥐 대장 평활근 세포에서 내향 정류성 칼륨 전류의 특성 연구
이은주,김명,정명섭,조향훈,김기훈,하현철,김준수,최석,전제열 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.2
Objectives: K^(+) channels play an important role in regulating cellular excitability. The aim of this study was to know whether or not inward rectifier K^(+) channel exists in colonic smooth muscle cells. Methods: Mouse colonic smooth muscle cells were isolated using collagenase, and then we recorded their membrane currents using a whole-cell patch clamp technique. Results: With 90 mM K^(+) in bath, hyperpolarization-induced inward currents from -120 mV to 20 mV with 400 ms duration at a holding potential of -10 mV showed rapid activation, inactivation and inward rectification. The inactivation showed single exponential time course. Reduction of external K^(+) to 60 mM decreased the amplitudes of the currents in whole test voltage range and shifted the reversal potential to more negative potential. The inactivation process and peak currents of hyperpolarization-induced inward currents were not affected by removing external Na^(+). External Ba^(+) blocked hyperpolarization-induced inward currents by dose-dependent manner and pure Ba^(+)-sensitive currents showed strong inward rectification. Cs^(+) also suppressed hyper- polarization-induced inward currents. Ba^(+) and Cs^(+)-induced inhibitiOn of hyperpolarization-induced inward currents was voltage-dependent, and the extent of inhibition increasing with membrane hyperpolarization. Conclusions: These results suggest that inward rectifier KU channels may exist in proximal colonic smooth muscle and may play an important role in regulating membrane potential.
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개 대뇌겉질에서 Platelet-Derived Growth Factor α-Receptor의 출생 후 발달에 관한 면역조직화학적 연구
윤영,안병수,김인정,양경철,박선홍,김기훈,박도영,김장만,문정석,장인엽,조하영 조선대학교 부설 의학연구소 2002 The Medical Journal of Chosun University Vol.27 No.1
Background and Objectives : The localization of platelet-derived growth factor-α receptor (PDGF-α R) was commonly restricted to oligodendrocyte progenitors during late embryonic and postnatal development. However, several studies recently demonstrated that mature neurons could also synthesize PDGF-α, Materials and Methods : In the present study, to analyze the distributional pattern of PDGF-αR during postnatal development of the canine cerebral cortex, we used immunohistochemistry on sections of canine brain tissue. Results : We found that neurons of various regions of cerebral cortex exhibited the immunoreactivity to PDGF-αR as early as postnatal day 0, and slightly decreased after postnatal day 14. Whereas neuronal PDGF-αR were maintained at all ages, the oligodendroglia-like expression of PDGF-αR could not be confirmed. Conclusion : The localization of PDGF-αR in immature and mature neurons supports the several roles of PDGF during development, protection and survival of neurons.
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