폐경기 골다공증 여성에 있어서 Alendronate 치료에 대한 골교체의 생화학적 표지자의 평가

김기석(Kie Suck Kim),민부기(Bu Kie Min),이승필(Seung Fil Lee),김인숙(In Suk Kim),김훈영(Hun Young Kim),심재량(Jae Ryang Sim) 대한폐경학회 2000 대한폐경학회지 Vol.6 No.1

N/A Objectives: To evaluate the clinical utility of recently developed biochemical markers of bone turnover to monitor the response of osteoporotic patients to antiresorptive therapy, we compared the results of two advanced assays for markars of bone resorption and two of bone formation. Subjects and Methods: The rate of bone turnover in 37 women (mean±SD age, 58.2±4.5yr) with low boe mass and all postmenopausal women (mean±SD yr PMP, 8.3±5.2) was compared to that in 16 Premenopausal women(mean+SD age, 40.2±5.3yr) randomly selected from out-patient in our hospital and all have a normal spine bone mineral density(BMD). Perodically during the 12-month study,the level of several markers of bone turnover wen, measured. Serum osteocalcin, bone specific alkaline phosphatase mesured by RIA were used to assess bone formation. To assess bone resorption, we measured urinary excretion of Dedxypyridinoline, type l collagen cross-linked N- telopeptide, Result: All bone formation markers and all bone resorption marker were significantly increased in PMP Osteoporotic women. Under treatment with alendronate, resorption markers decreased earlier than marker of bone formation, Conclusion: This study, using biochemical markers of bone turnover, demonstrates that bone turnover is increased in PMP osteoporotic women. Alendronate treatment decreased bone turnover to the normal premenopausal range, with a steady state level reached after 1 month of therapy with 10mg for resorption markers and after 3-6 months of therapy for markers of bone formation.

Estradiol과 Medroxyprogesterone Acetate가 골아세포의 성장에 미치는 영향

김기석 ( Kie Suk Kim ),민부기 ( Bu Kie Min ),홍기연 ( Gi Youn Hong ),박승택 ( Seung Taek Park ),이승필 ( Seung Phil Lee ),김인숙 ( In Suk Kim ) 대한폐경학회 2001 대한폐경학회지 Vol.7 No.1

N/A This study was designed to clarify the hormonal effect on the growth of osteoblast. And the osteoblasts from neonatal mouse were cultured in the medium at various concentrations of 17β-estradiol(EST), medroxyprogesterone acetate(MPA) in addition 10nM of dexamethasone(DMS) and alcohol for 48 hours. And in order to detect the response of osteoblast on the those hormones, the alkaline phosphatase(ALP) activity also was measured. 1. At 48 hours after culture of osteoblasts in media at various concentrations of hormones, EST has an effect on increasing of the cell number in fashion of dose dependent, whereas MPA did not increase the cell number. 2. Dexametasone significantly promoted differentiation of osteoblasts does dependently with increasing of ALP activity. 3. In osteoblasts culture in media supplemented with alcohol in the absence of 10nM DMS, EST increased significantly ALP activity, while decreased ALP activity of osteoblast in the presence of DMS, 4. In culture of osteoblasts treated with alcohol in the absence of 10nM DMS, MPA did not show any change of ALP activity. But in the presence of DMS, MPA decreased ALP activity of osteoblast. 5. EST plus MPA stimulated the ALP activity in osteoblasts treated with alcohol in the absence of DMS. While, in the cells treated with DMS, they synergistically decreased ALP activity.

NO(Nitric Oxide)가 생쥐의 배 발달에 미치는 영향

민부기,김기석,이희섭,홍기연,신형도,성연경,김형민,Min, Bu-Kie,Kim, Kie-Suk,Rhee, Hee-Sub,Hong, Gi-Youn,Shin, Hyeong-Do,Sung, Yeon-Kyeong,Kim, Hyung-Min 대한생식의학회 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.2

Objective: To ananlyze the direct effect of nitric oxide (NO), generated from sodium prusside (SNP) on the embryo developments in reproductive process. Design: Ova from mouse were treated to allow fertilization in in vitro culture. And the samples of fertilized ova were alloted into five alliqutos. Each alliquot was cultured in media treated with either concentration at 0 (n=92), $25{\mu}M$ (n=84), $50{\mu}M$ (n=80), $100{\mu}M$ (n=77), $500{\mu}M$ (n=54) of SNP. Main Outcome Measure: Rates of embryonal cell cleavages, viability and cell morphology were assessed during in vitro fertilization and culture. Results: As analyse the cell cleavage at 24 hours after in vitro culture of fertilised egg in variuos NO concentration, all of egg cells of each alliquot were developed to $2\sim4$ cell stage. But the alliquot of egg cells treated with $50{\mu}M$, which were totally degenerated. And also all embryonal cells of each alliquot were developed to 8 cell stage and morula stage on culture continuosly. And the embryonal cells of each alliquot were analysed at 24 and 48 hours following the in vitro culture. The rates of cell fragmentation and fusion were $4.2{\pm}3.4%$ in control group which is not treated with NO, while experimental groups was high, as rated $23.4{\pm}6.2%$ in $25{\mu}M$, $28.2{\pm}5.7%$ in $50{\mu}M$ and $32.1{\pm}6.4%$ in $100{\mu}M$ concentration of NO. Accordingly the rate of abnormal morphology of embryonal cell in control was lower significantly than that in each alliquot of experimental groups (p<0.05). And the degenerated rates of embryonal cells were 0% in control, $17.8{\pm}6.7%$ in $25{\mu}M$, $23.6{\pm}4.7%$ in $50{\mu}M$ and $26.8{\pm}11.2%$ in $100{\mu}M$ at 8 cells and morula on culture of 48 and 72 hours. On the examination of embryonal cells developed to blastocyst through in vitro culture, the rates of degenerated cells were $16.8{\pm}7.2%$ in control, $37.5{\pm}6.2%$ in $25{\mu}M$, $73.4{\pm}4.6%$ in $50{\mu}M$, 100% in $100{\mu}M$. Conclusion: This results suggeted that the NO in any concentrations is harmful on embryos in view of morphology as well as viability of cell, and the toxicity of NO on embryo is stronger at condition in higher concentration of NO.

Leukemia Inhibitory Factor가 배의 배포형성에 미치는 영향

민부기,오수미,김기석,홍기연,김훈영,심재량,박승택,Min, Bu-Kie,Oh, Soo-Mi,Kim, Kie-Suk,Hong, Gi-Youn,Kim, Hun-Young,Sim, Jea-Ryang,Park, Seung-Teak 대한생식의학회 2001 Clinical and Experimental Reproductive Medicine Vol.28 No.1

Objective: To determine the effects of leukemia inhibitory factor (LIF) on embryonal development in in vitro culture. Methods: This is designed in vitro model using eggs from mouse. The eggs from mouse were assigned 29 for control group, 53 for 20 ng/ml of LIF, 88 for 40 ng/ml of LIF, 68 for 80 ng/ml of LIF respectively for in vitro fertilization. And 26 fertilized eggs at 2 cell stage from mouse also were assigned. The mouse embryos of all groups were cultured in medium supplemented with LIF in different concentrations, whereas the eggs in control group was cultured in medium without supplement of LIF. Results: At 72 hours culture of eggs from in vitro fertilization, there was a slight increas in rate of embryonal development to morula in both LIF-20 and LIF-40 as results of 64.15% and 75% respectively, while 42.65% in inferior rate of LIF-80, compare with 51.72% in control group. But the difference between these each groups were not significant in statistically ($p{\le}0.05$). And after 96 hours culture of eggs, the rates blastocyst formation was significantly higher in both LIF-20 and LIF-40 as 56.6% and 63.63% than those in control and LIF-80 as 44.83% and 35.29% respectively. On culturing eggs from in vivo fertilization, the rates of blastocyst formation was significantly not only higher as 85% and 81.81% respectively in medium supplemented with LIF-40 and LIF-80 than 42.3% in LIF-20 but also embryonal cell viability were remakedly improved at 96 hours after culture. Conclusion: The LIF in low dose is embryotrophic, but LIF in high dose is embryotoxic on eggs from in vitro fertilization. Whereas on culturing eggs from in vivo fertilization, LIF is more beneficial with dose dependent in high concentration.

체외배양에서 인간 난포액이 생쥐의 배 발달에 미치는 영향

민부기,최기욱,김기석,이희섭,홍기연,이봉주,이선영,박승택,Min, Bu-Kie,Choi, Ki-Wook,Kim, Kie-Suk,Lee, Hee-Sub,Hong, Ki-Yeon,Lee, Bong-Ju,Lee, Sun-Young,Park, Seung-Teak 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.2

The follicular fluid (FF) of ovary contains various biological active products which affected on the growth of follicles and the fertilization of oocyte in physiological reproductive process of mammals. This study was designed to determine the effects of human FF on fertilization of oocyte and embryonal development in vitro culture. The FF was prepared as clear without blood contamination by needle aspiration from mature follicles of human at the time of oocytes retrieval for in vitro fertilization (IVF). As the medium for culture in vitro of embryonal cells, human tubal fluid (HTF) supplemented with follicular fluids at concentrations of 10%, 40% and pure FF were used. These effects were compared to control group of cultured embryos in HTF supplemented with 0.4% BSA (bovine serum albumin). For IVF, 64 eggs in control group, 67 eggs in 10% FF, 57 eggs in 40% FF and 64 eggs in pure FF were respectively allocated. And the rates of fertilization were almost similar in all groups as resulting 82.81% in control, 85.07% in 10% FF, 87.71% in 40% FF and 81.25% in pure FF. On the examination for embryonal cleavage from fertilized eggs, the rates of developing to 4 cell stage was similar in all groups, as results 98.11% in control, 98.27% in 10% FF and 98% in 40% FF but 78.84% in pure FF. And the rates of developing to 8-16 cell stage were significantly reduced as 44% in 40% FF and 44.23% in pure FF (p<0.05) compare to 71.69% in control media. As likewise, the rates of developing to morular stage were also significantly reduced to 36% (p<0.05) and 21.15% (p<0.01) respectively in 40% FF and pure FF. And the rates to blastocystic stage of embryo was lowest as 7.69% in pure FF (Table 1). The quality of embryonal cells on cleavage to the 8-16 cell stage was poorer, higher concentrations of FF. The rates of grade 1 in pure FF, as 23.07%, was lowest compare to those of other groups, in which the rates of grade 1 in control, 10% FF and 40% FF were 58.49%, 47.36% and 34% respectively. And on the contrary, the rate of grade 4 in pure FF was highest as 23.07%, while those were 5.66% in control, 8.77% in 10% FF and 20% in 40% FF (Table 2). On the viability of embryos, the rate of embryonal cell death was more rise, at the higher concentrations as well as longer exposure in the follicular fluid. At 48 hours after in vitro culture of embryos, the rate of survival embryos in pure FF was markedly lowered as 44.23%, compare to that of control (p<0.05). But there was not significant difference between the rates of survival embryos in each group beside the pure FF, which the rates were 77.35% in control, 70.17% in 10% FF and 60% in 40% FF respectively. And at 72 hours after in vitro culture, the rates of survival embryos were also significantly dropped to 21.15% in pure and 36% in 40% at concentration of FF compare to 62.26% in control (p<0.05, p<0.01). Finally, the rate of embryonal death at 96 hours after in vitro culture was highest as 82.69% in pure FF among all groups which those were 35.84 in control, 56.14% in 10% FF and 64% in 40% FF respectively (Fig. 1, 2, 3). In conclusion, this study suggests that the FF has no effects, in particular, to the in vitro fertilization of oocytes but exerted a bad effect to the cleavage, quality and viability of the embryonal cells during in vitro culture. However, the FF is harmful on embryonal development at conditions in higher concentration and especially on the embryos after $8{\sim}16$ cell stage.

정상 임신과 임신성 고혈압 환자에서의 혈장 Fibronectin 농도에 관한 연구

홍기연 ( Gi Youn Hong ),박성찬 ( Sung Chan Park ),김창홍 ( Chang Hong Kim ),이희섭 ( Hee Sub Rhee ),민부기 ( Bu Kie Min ),김기석 ( Kie Suk Kim ) 대한주산의학회 1992 大韓周産醫學會雜誌 Vol.3 No.2

임신성 고혈압증의 임상적 고찰

홍기연 ( Gi Youn Hong ),오수미 ( Su Mi Oh ),박현진 ( Hyun Jin Park ),신형도 ( Hyung Do Shin ),이희섭 ( Hee Sub Rhee ),김홍곤 ( Heung Gon Kim ),민부기 ( Bu Kie Min ),김기석 ( Kie Suk Kim ),김혜정 ( Hae Chung Kim ) 대한주산의학회 1999 大韓周産醫學會雜誌 Vol.10 No.4

자궁경관 무력증에 관한 임상적 고찰

민부기,송정,국승무,최영원,김기석 圓光大學校 醫科學硏究所 1985 圓光醫科學 Vol.1 No.2

This is the report of a retrospective study of 42 cases in cervical incompetence, identified clinically by the department of Obstetrics and Gynecology at won Kwang University Hospital during the period of 4 years from March, 1981 through march, 1985. All 42 cases were divided as variety of operative techniques such as Mc Donald’s, 33 cases, Shirodkar’s, 5 cases, and Lash’s, 4 cases. The 38 cases of cervical incompetence on pregnant state were divided again by group 1, 30 cases of which cervical os were closed or opened as 1.5cm, by group 2, 8 cases of which cervix is effaced as 50 % or more dilated as 3 cm or more. In the age distribution of cervical incompetence, the range from 30 years to 34 years is most common as 21 cases among the 42 cases of I.I.O.C. On analysing by past history in 42 cases of cervical incompetence, the 25 cases, 59 % had one or more of artificial abortion, in 6 cases, birth trauma, in 6 cases, repeat cervical cerclage respectively. And the others are cervical conization, cervical cauterization, and previous C-section. On comparisons between methods of operation and success rate of fetal salvage, the success rate following the Me Donald’s operation is 84% in series of the 28 cases among the 33 cases, and the success rate of 100% following the Shirodkar’s in series of the 5 cases among the 5 cases, 75 % following the Lash and Lash’s. In the hospital days following the cervical cerclage, 19 cases remained in hospital for 5 days, for 5 days more to 7 days in 11 cases, and for 8 days more 10 days in 6 cases. All the period of the hospital days is considerably longer in group 2 than in group 1. The comparison between operative time and success rate, when cervical cerclage is performed before 17 weeks of pregnancy, is seen 100% of success rate, but in cases of cervical cerclage after 18 weeks of pregnancy is 66.7% as 14 cases of 21 cases. The complication rate following cervical cerclage is 13.2% as 5 cases of all 38 cases.

난소종양환자에서 수술전 CA - 125 측정의 유용성

이승준,이영규,김흥곤,홍기연,민부기,김기석 대한부인종양 콜포스코피학회 1995 Journal of Gynecologic Oncology Vol.6 No.2

This study was undertaker to define the usefulness of preoperative CA-125 assay as a diagnostic tumor marker in differentiating malignancy from benign ovarian mass. Serum CA - 125 were measured by Microparticle Enzyme Immunoassay(MEIA) in 94 patients with ovarian mass. The results were of follows ; 1. The mean value of preoperative serum CA-125 was 18.40u/ml in benign ovarian mass and 225.99u/ml in malignant ovarian mass(P$lt;0.001). 2. The positive rate of Ca-125 in benign ovarian mass was 10%, compared 80% in malignant ovarian mass. 3. In analysis of histologic type, positive rate of serum CA-125 in malignant serous tumor was 82%, compared 50% in malignant mucinous tumor. 4. No statistically significant correlation was observed between CA - 125 value and patient's age. 5. The sensitivity, specificity, positive predictive value & negative predictive value were 80%, 90%, 60% & 96%, respectively in cut off value, 35u/ml, And increasing cut off value 65u/ml, sensitivity, specificity, positive predictive value & negative predictive value were 40%, 96%, 67%, 90%, respectively. These data suggest the preoperative serum CA-125 level correlate with maignant status in ovarian mass. And cut off value 35u/ml was better than 65u/ml in screening for ovarian cancer.