어피 젤라틴 펩티드와 결합한 굴껍질 유래 칼슘 화합물이 칼슘 결핍 흰쥐에 미치는 영향

김규형(Gyu Hyung Kim),전유진(You Jin Jeon),변희국(Hee Guk Byun),이연숙(Yeon Sook Lee),이응호(Eung Ho Lee),김세권(Se Kwon Kim) 한국수산과학회 1998 한국수산과학회지 Vol.31 No.2

To utilize the oyster shell containing a lot of calcium, we investigated the bioavaliability of calcium compounds from oyster shell. First, calcium oxide was prepared by burning of oyster shell at 1200℃. Its purity was approximately 98.5%. Calcium compounds, CaCl₂ and CaHPO₄ from the calcium oxide were prepared by chemical reaction. Effect on calcium absorption by the calcium compounds from oyster shell was improved using fish skin gelatin peptides,(FSGP), which was prepared by enzymatic hydrolysis of fish skin gelatin for 4hr with tuna pyloric caeca crude enzyme(TPCCE). In vitro experiment, calcium absorption by addition of FSGP in a mixture solution of calcium and phosphate was higher approximately 70% than that by control. In vivo using calcium deficient rats, a group taken the diets with 3% FSGP and CaHPO₄ was significantly improved amount of calcium and ash in femur and strength of femur. These results suggest that calcium compounds from oyster shell and FSGP could be used as an effective dietary calcium source.

성게로부터 분리한 β-galactosidase 의 정제 및 특성

김규형(Gyu Hyung Kim),김용태(Yong Tae Kim),김세권(Se Kwon Kim) 한국수산과학회 1998 한국수산과학회지 Vol.31 No.5

β-Galactosidase was extracted from the internal organ of sea urchin, Hemicentrotus pulcherrimus. The enzyme was purified 384.6-fold over the crude extract by the sequential chromatographic methods including DEAE-Sephadex A-25, CM-Cellulose, and Con A-Sepharose 4B affinity chromatography with a recovery 1.26%. The molecular weight of the purified enzyme was estimated approximately 94 kDa as monomeric form by SDS-PAGE and Sephadex G-150 gel chromatography. The maximum enzymatic activity was observed at pH 3.0 and 50℃, but the enzyme was stable over the pH range of 3.0∼5.0 and below 37℃. The K_m and V_max values against PNPG (p-nitrophenyl β-D-galactopyranoside) were-15.0 mM and 214μmole/min per mg protein, respectively. The enzymatic activity was activated by Ba^(2+), butt significantly inhibited by DFP, Hg^(2+), Sn^(2+) and galactose.

우측 수부의 모든 수지 절단 환자에서 무지와 제 2, 3 수지 재건술

이준모,김규형,Lee, Jun-Mo,Kim, Gyu-Hyung 대한미세수술학회 2001 Archives of reconstructive microsurgery Vol.10 No.2

전북대학교병원 정형외과에서 우측 제 1-5 수지가 모두 절단된 35세 남자에서, 우 수부 무지는 포장주위 피판(wrap around flap)시 제 1 배부 중수골동맥(the first dorsal metacarpal artery)의 고유 동맥(proper digital artery)을 제 1 배부 중족골 동맥(the first dorsal metatarsal artery)에, 두 정맥 분지(tributary of the cephalic vein)는 대 복재 정맥의 분지 (tributary of the greater saphenous vein)에 각각 문합하였고, 수부의 요골신경의 감각 분지는 거상된 포장 주위 피판의 심부비골 신경(deep peroneal nerve)에 신경 외막 봉합술을 시행하였다. 제 2, 3 수지 재건술에서는, 우 2, 3 족지의 족 배 동맥을 요골 동맥에, 대, 소 복재 동맥의 분지를 두 개의 두 정맥 분지에 각각 단-단 문합하였으며, 우 제 2, 3 족지의 표재 비골 신경을 요골 신경 분지에 신경 외막 봉합하였고, 동시에 우 제 2, 3족지의 지골 신경을 우 2, 3 수지의 지골 신경에 신경 외막 봉합하고 7년 추시하였으며, 우 수부 무지와 제 2, 3수지를 이용한 식사하기, 글씨 쓰기 그리고 양말 신기 등의 동작이 가능하였다. The hand with amputation of all fingers is useless for activities of daily living and traumatic amputation of some of the fingers can result in the diminished ability to perform power grip and precision grip which is vital to maintain normal function of the hand. Precision grip is used to hold an object between the opposable thumb and flexed fingers. In power grip the object is held between the flexed fingers and the palm while the thumb applies the necessary counterpressure to maintain the grip on the object. A 35 year old male lost his right all fingers including thumb at the level of proximal phalanx from the pressure machinary accident. Thumb was reconstructed using wrap around flap and the second and third fingers were reconstructed using the second and third toe transplantation. Seven years after reconstruction, he uses the reconstructed thumb and the second and the third fingers for eating meals, writing down a paper with a pencil and putting on socks.

한외여과막 효소반응기를 이용하여 제조한 키토산 올리고당의 칼슘흡수 촉진효과

전유진(You Jin Jeon),김규형(Gyu Hyung Kim),박표잠(Pyo Jam Park),김세권(Se Kwon Kim) 한국수산과학회 1999 한국수산과학회지 Vol.32 No.3

In spite of various bio-functionalities of chitosan, the effects in vivo were still ambiguous because of its low absorption on organism. Therefore, chitosan oligosaccharides(COSs) are necessary to elucidater for an efficient utilzation in vivo. COSs were prepared from chitosan using ultrafiltation membrane enzymatic reactor system with MWCO(molecular weight cut-off) 3,000 Da of membrane. Calcium absorption accelerating effect using COSs was examined by two methods, in vitro and in vivo. In vitro experiment, calcium control. In vivo using rats, group taken the diet contained 1% COSs and calcuim choride 1% COSs and calcium chloride decreased about 75% of calcium content excreted from feces, and then, showed about 15% increase in breaking farce of femur. These results demonstrated that COSs desnitelr involved in calcium metabolism in vivo.

민태 Frame 단백질 유래의 인산화 및 비인산화 펩타이드가 체내 칼슘 흡수에 미치는 영향

김세권(Se Kwon Kim),전유진(You Jin Jeon),변희국(Hee Guk Byun),박표잠(Pyo Jam Park),김규형(Gyu Hyung Kim),최영일(Yong Ri Choi),이연숙(Yeon Sook Lee) 한국수산과학회 1999 한국수산과학회지 Vol.32 No.6

In order to utilize rotein hydrolysate produced from hoki (Johnius belengeri) frame among many different fish processing wastes, hoki frame peptide (HFP) and phosphorylated hoki frame pep6de (PHFP) were prepared, and their calcium absorption accelerating effects were investigated in comparison to control and casein phosphopeptide (CPP). In in vitro experiment, HFP and PHFP inhibited calcium phosphate formation as high as 1.5-fold and 2.5-fold, respectively, comparing to control. In addition, the inhibition rate of calcium phosphate precipitation as increasing concentrations of HFP and PHFP was risen and was similar to that of CPP at 2.0 ㎎/ℓ of PHFP concentration. In in vivo experiment using the rats, the groups fed HFP and PHFP indicated significantly increased calcium content in the femur. In particular, the calcium content in the small intestine of the rat fed PHFP was higher than that of control group by approximately 60%.

대구 Frame 단백질 가수분해물을 이용한 효소분해간장의 제조

김세권(Se-Kwon Kim),박표잠(Pyo-Jam Park),김규형(Gyu-Hyung Kim) 한국식품영양과학회 2000 한국식품영양과학회지 Vol.29 No.4

본 연구에서는 폐기되거나 사료로 이용되고 있는 대구 frame에 다량으로 포함되어 있는 단백질로부터 쓴맛이 없는 효소분해 간장을 개발하기 위해 참치유문수 유래의 조효소를 추출하고 이를 이용하여 대구 frame을 가수분해시켰으며, 한외여과막을 사용하여 분자량별로 분획하여 조미간장을 제조한 후 간장으로서의 이용가능성을 검토하였다. 한외여과막을 사용하여 분자량별로 분획한 각각의 가수분해물들은 25,000 kDa 이하에서 서로 유사한 분자량 분포를 보였으나, 분획에 사용된 막의 한계분자량에 따라 분포 비율은 서로 차이를 보였다. 또한, 아미노산의 조성분석에서도 각 가수분해물에 포함되어 있는 아미노산의 함량 차이는 거의 없었으나, 1 K 가수분해물에서 유리아미노산의 함량이 상대적으로 높았다. 분자량별로 분획된 TPCCE 가수분해물들의 관능평가 결과 1 K가수분해물이 모든 항목에서 가장 우수한 것으로 나타났으며, 이를 원료로 하여 효소분해간장을 만들어 다시 관능평가한 실시한 결과, 1 K 가수분해물로 제조한 간장은 육단백질을 효소분해할 때 나타나는 쓴맛은 사라졌지만 시판용 간장과 맛의 비교평가에서 약간 낮은 점수를 얻었다. 그러나 효소분해간장 원액을 시판 양조간장과 일정한 비율로 섞어 관능평가를 실시하였을 때는 모든 항목에서 우수한 점수를 얻었다. 따라서, 1 K 가수분해물로 제조한 효소분해간장과 양조간장을 일정비율로 혼합할 경우, 현재 시판되는 산분해 혼합간장의 안정성을 향상시킬 수 있을 것으로 판단된다. In order to utilize the protein source from a fish processing by-product, cod frame was hydrolyzed with various enzymes such as tuna pyloric caeca crude enzyme (TPCCE), α-chymotrypsin, trypsin, papain and pronase E. The TPCCE hydrolysate acquired the highest sensory properties on taste, odor and color. The resultant cod frame protein hydrolysate (CFPH) which was hydrolyzed with TPCCE, was separated through a series of ultrafiltration membranes with molecular weight cut-off (MWCO) of 30, 10, 5 and 1 kDa, and four types of permeates including 30 K (permeate from 30 kDa membrane), 10 K (permeate from 10 kDa membrane), 5 K (permeate from 5 kDa membrane) and 1 K (permeate from 1 kDa membrane) were obtained. The natural sauces were prepared with 30 K, 10 K, 5 K and 1 K hydrolysate, and the sauce prepared with 1 K hydrolysate was the best score in sensory evaluations. In addition, the mixed sauce prepared with 1 K hydrolysate and commercial soy sauce was similar to commercial sauce in sensory properties. These results suggest that the mixed sauce would be utilized as the substitute of acid-hydrolysis sauce.

유동유발진동에 의한 APR1400 증기발생기 내부구조물의 구조적 건전성 평가

김동학(Dong Hak Kim),김규형(Kyu Hyung Kim),임상규(Sang Gyu Lim) 대한기계학회 2011 대한기계학회 춘추학술대회 Vol.2011 No.10

In this paper, structural integrity assessment of steam generator internals such as separator assembly and dryer assembly by flow-Induced vibration is conducted for APR1400 nuclear power plant. The allowable stress limits are selected using design fatigue curves of ASME. The structural integrity assessment is conducted by comparing the allowable stress limits with the maximum stresses determined by random vibration analysis. PSD(power spectral density) analysis for random vibration analysis is conducted with random hydraulic loads due to steam flow and results of modal analysis. Random hydraulic load is determined in terms of PSD function using the velocity calculated by the transient analysis. The modal analysis is conducted using Block Lanczos method. All of mode shapes within range of 0~100Hz are used for PSD analysis. As the result of integrity assessment due to flow-induced vibration, APR1400 steam generator internals have sufficient safety margins.

단발성 섬유성 골 이형성증을 동반한 경추의 병적 골절

송경진(Kyung-Jin Song),김규형(Gyu-Hyung Kim) 대한정형외과학회 2008 대한정형외과학회지 Vol.43 No.2

섬유성 골 이형성증은 골의 발육 이상으로 해면골과 골수가 섬유성 조직 및 작은 직골로 대치되어 나타나는 질환으로 30세 이하의 연령에서 주로 호발하며, 남자보다 여자에서 약간 많이 발생한다. 한 뼈에만 나타날 때는 단발성, 여러 뼈에 나타날 때는 다발성으로 분류 할 수 있으며 경추부에 발생하는 섬유성 골 이형성증은 매우 드물게 보고되고 있다. 이에 저자들은 경추부에 발생한 단발성 섬유성 골 이형성증에 의해 제4와 제5 경추의 병적 골절에 의한 후만 변형이 동반된 1예를 경험하였기에 보고하고자 한다. Fibrous dysplasia is a developmental anomaly of bone formation, which is characterized by a replacement of the normal bone and marrow by fibrous tissue as well as small, woven spicules of bone. These lesions may exist in a monostotic or polyostotic form and are generally found in the 4<SUP>th</SUP> decade of age or younger with a slightly higher incidence in girls than boys. We report a case of monostotic fibrous dysplasia that was associated with a pathologic fracture and a kyphotic deformity in the cervical spine.

원자로 축소모델시험 동압계 배치

고도영(Do-young Ko),김규형(Kyu-hyung Kim),임상규(Sang-gyu Lim),이재민(Jae-min Lee),이도환(Do-hwan Lee) 한국소음진동공학회 2020 한국소음진동공학회 학술대회논문집 Vol.2020 No.11

경추부 신전손상의 연부조직 손상

송경진(Kyung-Jin Song),김규형(Gyu-Hyung Kim),왕성일(Seong-Il Wang),이주홍(Ju-Hong Lee) 대한정형외과학회 2007 대한정형외과학회지 Vol.42 No.4

목적: 신전손상에 의한 하경추부 외상에서 연부조직의 손상을 MRI로 분류하여 신전손상의 정도를 진단하고 또한 연부조직 손상 정도와 척수 손상의 발생이 관계가 있는지 알아보고자 하였다. 대상 및 방법: 최근 5년간 하경추부 신연-신전손상으로 진단되어 치료를 받은 81명을 대상으로 하였다. 모든 환자에서 술 전 MRI를 촬영하였으며 연부조직 손상의 정도를 5단계로 세부 분류하였다. 결과: 후종 인대 파열이 동반된 3단계 이상의 연부조직 손상에서 척수 손상이 발생되었다(p<0.01). 척수 신호 강도의 변화도 연부조직 손상 단계가 증가할수록 많이 발생하였다(p<0.01). 척추관 협착증 환자에서 연부조직 손상과 척수 손상과는 관계가 없었다(p=0.45). 결론: 하경추부 신연-신전 손상에서 자기공명영상은 연부조직 손상의 정도를 정확하게 진단할 수 있었으며 연부조직 손상의 정도와 척수손상의 발생은 밀접한 관계가 있음을 알 수 있었다. Purpose: To diagnose the extent of soft tissue damage with MRI, and to evaluate the relationship between soft tissue damage and a spinal cord injury in an extension injury to the lower cervical spine trauma. Materials and Methods: Eighty-one patients who treated surgically for an anextension injury to the cervical spine over the past 5 years, were analyzed. All patients had undergone MRI after the injury, and for the specific grading of soft tissue damage, the grades were defined from grades 1 to 5. Results: The spinal cord injury developed with more than grade 3 soft tissue damage associated with a rupture of the posterior longitudinal ligament (p<0.01). The changes in signal intensity of the spinal cord also developed according to the severity of soft tissue damage (p<0.01). There was no relationship between the soft tissue damage and the spinal cord injury in spinal stenosis (p=0.75). Conclusion: The extent of soft tissue damage was diagnosed precisely with MRI, and there was an close relationship between the soft tissue damage and spinal cord injury in the distractive-extension injury to the lower cervical spine trauma.