Amplification and Expression of Several Cellular Proto-Oncogenes in Squamous Cell Carcinoma Cell Lines

김경순,박노희,Kim, Kyung-Soon,Park, No-Hee 생화학분자생물학회 1990 한국생화학회지 Vol.23 No.2

사람의 정상적 epidermal keratinocyte(NHEK)와 사람의 squamous cell carcinoma cell line에서 여러 가지 cellular proto-oncogene의 존재, restriction profile, 그리고 expession을 조사하였다. NHEK와 cell line들로부터 얻은 발암유전자의 Eco RI restriction profile은 유사하였고 정상세포와 암세포들은 모두 몇 가지 proto-oncogene들을 발현하였으나 NHEK에 비해 암세포에서 이들 proto-oncogene의 발현되는 양이 많았다. 이 결과로부터 squamous cell carcinoma의 malignant transformation과 그 유지에 있어서 몇 가지 cellular proto-oncogene들의 과발현이 필요하다는 것을 알 수 있다. The restriction profiles and expression of various cellular proto-oncogenes in normal human epidermal keratinocytes and human squamous cell carcinoma cell lines were studied. Both normal human epidermal keratinocytes and cancer cell lines contained and expressed various proto-oncogenes such as erb-B-1, abl, fos, raf, H-ras, and myc. Although the Eco RI restriction profiles of the oncogenes from the normal cells and cancer cells were similar, the amount of the oncogene in the cancer cell lines was generally higher than that in the NHEK. Furthermore, the amount of expression of these proto-oncogenes was significantly higher in the cancer cell lines than in the normal keratinocytes. These data indicate that the overexpression of several cellular proto-oncogenes might be necessary for the malignant transformation and maintenance of the malignancies of squamous cell carcinoma.

Adsorption Behaviors of Indomethacin on Cholestyramine Resin

김경순,Kim, Kyung-Soon 한국약제학회 1988 Journal of Pharmaceutical Investigation Vol.18 No.3

In vitro studies were performed on the interaction of indomethacin with cholestyramine, a hypocholesterolemic substance. Cholestyramine showed a marked affinity for indomethacin among tested acidic drugs and the intensity of adsorption was dependent on pH, temperature and sodium chloride. Moreover, the combination of indomethacin with some acidic drugs that formed complexes with cholestyramine, considerably inhibited the adsorption of indomethacin on the resin.

항암플러스 투여로 호전된 진행성 비소세포성 폐암 연속환자증례

김경순,정태영,유화승,이연월,조종관,Kim, Kyung-Soon,Jung, Tae-Young,Yoo, Hwa-Seung,Lee, Yeon-Weol,Cho, Chong-Kwan 대한한방내과학회 2009 大韓韓方內科學會誌 Vol.30 No.4

Objective : To investigate the therapeutic effects of Hang-Am-Plus (HAP) on advanced non-small cell lung cancer (NSCLC) patients. Method : We prescribed HAP three times a day (3,000-6,000 mg/ day) during the treatment period (8 - 24 months). Computed tomography (CT) was performed to evaluate the therapeutic efficacy. Results : Four patients who were diagnosed with NSCLC were recommended chemotherap y, but refused it because of old age, side effects, or treatment failure, and instead sought oriental medicinal therapy. They were treated around 12 months. The patients showed stable disease (SD) state for 5 months, 3 months, 19 months and 3 months, respectively. Conclusion : These four cases may give us the possibility that HAP offers potential benefits for non-small cell lung cancer patients.

괄루인 렉틴의 특성

김경순,최근호,박종옥,Kim, Kyung-Soon,Choi, Gun-Ho,Park, Jong-Ok 생화학분자생물학회 1994 한국생화학회지 Vol.27 No.5

Trichosanthis fructus Lectin (TFL) was partially purified by ammonium sulfate fractionation, heat treatment, DEAE-Sephadex ion exchange chromatography and Sephadex G-200 gel chromatography. The molecular weight of TFL was estimated by gel filtration and SDS-PAGE. The Molecular size of the lectin was determined to be 67,000 dalton, which was composed of two subunits having molecular weights of 34,000 and 35,000 dalton, respectively. The effect of sugars and metal ions on the hemagglutinating activity of lectin was also examined. The hemagglutinating activity of the lectin was inhibited by some sugars (${\beta}$-Lactose, ${\alpha}$-Lactose, D-(+)-Galactose) and metal ions ($Hg^{2+}$, $Cu^{2+}$, $Mg^{2+}$, $Ca^{2+}$, $Pb^{2+}$). 한국 중남부에 서식하는 다년생 박과 덩쿨식물인 하눌타리(Trichosanthes kirilowii) 씨앗인 괄루인(Trichosanthis fructus)으로부터 열처리, 황산 암모늄 분별침전, DEAE Sephadex 이온교환 크로마토그래피, Sephadex G-200 겔 크로마토그래피 등으로 렉틴을 정제하여 성질을 조사하였다. 렉턴은 2.3%의 수율로 60.4배 정제되었다. 겔 크로마토그래피로 측정한 분자량은 약 67,000 dalton, SDS-PAGE로 측정한 분자량은 34,000과 35,000 dalton의 두가지로 나타났으므로 이 렉틴은 dimer로 판단된다. Trichosanthis fructus 렉턴(TFL)은 galactose, ${\beta}$-lactose, ${\alpha}$-lactose 등의 당과 $Hg^{2+}$, $Cu^{2+}$, $Mg^{2+}$, $Pb^{2+}$ 등의 금속이온에 의하여 활성이 저해되었고 $40^{\circ}C$에서 15분간 유지시킨 결과 활성의 80%가 남았다.

Squamous Cell carcinoma cell line 에서 cellular proto - oncogene 들의 증폭 및 발현

김경순,박노희 ( Kyung Soon Kim,No Hee Park ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.2

The restriction profiles and expression of various cellular proto-oncogenes in normal human epidermal keratinocytes and human syuamous cell carcinoma cell lines were studied. Both normal human epidermal keratinocytes and cancer cell lines contained and expressed various proto-oncogenes such as erb-B-I, abl, fos, raf, H-ras, and myc. Although the Eco RI restriction profiles of the oncogenes from the normal cells and cancer cells were similar, the amount of the oncogene in the cancer cell lines was generally higher than that in the NHEK. Furthermore, the amount of expression of these proto-oncogenes was significantly higher in the cancer cell lines than in the normal keratinocytes. These data indicate that the overexpression of several cellular proto-oncogenes might be necessary for the malignant transformation and maintenance of the malignancies of syuamous cell carcinoma.

귀전우, 백화사설초, 와송 추출물을 처치한 난소암과 자궁경부암 세포에서의 단백질 발현 변화

김경순,예성철,유병철,조종관,이연월,유화승,Kim, Kyung-Soon,Yea, Sung-Chul,Yoo, Byong-Chul,Cho, Chong-Kwan,Lee, Yeon-Weol,Yoo, Hwa-Seung 대한한방내과학회 2011 大韓韓方內科學會誌 Vol.32 No.1

Background : Despite recent advances in cancer management, prognosis of ovarian cancer is poor. Anticancer effects of herbal medicine, such as Euonymus alatus Sieb, Oldenlandia diffusa (Willd.) Roxburgh, and Orostachys japonicus A. Berger, have been reported in treatment of ovarian and cervical cancers, but the systematic approaches to explain their molecular mechanism(s) have not yet been established. Objectives : To establish a basis of understanding for anti-cancer mechanisms of herbal medicine, we profiled protein expression in human ovarian and cervical cancer cells treated with the extracts from Euonymus alatus Sieb, Oldenlandia diffusa (Willd.) Roxburgh and Orostachys japonicus A. Berger. Methods : Human ovarian cancer cell line NIH:OVCAR-3, and human cervical cancer cell line HeLa were employed in the present study. Whole protein was obtained from the cells harvested at 48 hours after the treatment with herbal water-extract, and analyzed by 2DE-based proteomic approach. Results : Various changes of protein expression induced by the herbal treatment were monitored : down-regulation of molecular chaperone (calreticulin variant), glycolytic enzymes (D-3-phosphoglycerate dehydrogenase, glyceraldehyde 3-phosphate dehydrogenase and alpha-enolase), RNA processing molecules (hnRNP A2/B1), and antioxidant protein (peroxiredoxin 1). Conclusions : Repression of glycolysis has been accepted as the mechanism to increase anticancer reagent's effect. Thus, down-regulation of glycolytic enzymes by the herbal extracts suggested a possible synergistic effect of herbs in the presence of platinum-based therapeutics. In further study, as well as the synergistic effect of the herbs, it has to be further validated whether artificial regulation of hnRNP A2/B1 in ovarian cancer cells affects various cancer survival factors, since RNA processing can be interrupted by deranged expression of hnRNP subtypes, and it results in an inhibition of cancer cell growth.

항암단 투여로 호전된 진행성 또는 전이성 담낭암 연속환자증례

김경순,유화승,정태영,이연월,조종관,Kim, Kyung-Soon,Yoo, Hwa-Seung,Jung, Tae-Young,Lee, Yeon-Weol,Cho, Chong-Kwan 대한한방내과학회 2010 大韓韓方內科學會誌 Vol.31 No.3

Objective : To investigate the therapeutic effects of Hang-Am-Dan(HAD) on gallbladder cancer patients. Method : We prescribed HAD three times a day(3,000-6,000 mg/day) during a treatment period of 8 and 34 months respectively. Computed tomography(CT) was taken to evaluate the therapeutic efficacy. Results : Two patients diagnosed with gallbladder cancer were recommended to go through chemotherapy. However, they refused the standard regimen due to their old age and the fear of side effects, and chose to go with Oriental medicine treatments instead. The patients were treated for 8 and 34 months respectively. Each patient showed stable disease(SD) state during the treatment period. Conclusion : According to these cases, it could be concluded that HAD may offer potential benefits in treating gallbladder cancer patients.

동해 시 아파트 공급의 시대적 변천에 관한 연구

김경순,Kim, Kyung-Soon 한국주거학회 2006 한국주거학회 논문집 Vol.17 No.5

The purpose of this study was to explore the periodic changes of apartment supply between 1980 and 2001 in the City of Donghae. A survey was conducted with 82 apartment complexes that were built in during 21years. The data were itemized and specified by the unit house area, story, periodic and local apartment distribution. They were classified by five:(5) periods and analyzed by each apartment complexes for building characteristics. There were found that apartment housing were influenced by many primary factors such as demographics and a lot of the housing politics etc. The results of this study were as follows: 1) Apartment supply in the City of Donghae mass-produced from 1980 to 2001; Especially the forth period was supplied 5,999 households. 2) Between south area and north area was found a regional disparity. 3) The exclusive use $34{\sim}66m^{2}$ were continuously and centrally supplied according to the housing policy and practical demand. 4) For the types of story were variously found ; Especially the below 5 story apartment was continuously supplied.

Baker's Yeast 로부터 1,4-Benzoquinone Reductase 의 분리

김경순,석희원 ( Kyung soon Kim,Hee won Suk ) 한국유화학회 1997 한국응용과학기술학회지 Vol.14 No.3

An intracellular, soluble 1,4-benzoquinone reductase was purified from Baker`s Yeast by ammonium sulfate precipitation, DEAE-Sephacel anion exchange chromatography, and Sephacryl S-200 gel filtration chromatography. 1,4-Benxoquinone reductase was achieved 123.8 fold purification from crude homogenate with a yield of 11.1%.

난소암 치료에 대한 한의 임상 가이드라인

김경순,최홍식,김승모,유화승,Kim, Kyung-Soon,Choi, Hong-Sik,Kim, Seung-Mo,Yoo, Hwa-Seung 대한암한의학회 2016 大韓癌韓醫學會誌 Vol.21 No.2

Ovarian cancer is the tenth most common cancer in Korean female, and the third most common cancer of female reproductive organ after breast cancer and uterine cervical cancer. In spite of develop of conventional treatment, high modality of ovarian cancer comes from difficulty of an early diagnosis. Recent studies revealed that combining conventional and integrative medical treatment can reduce the adverse effect of surgical operation, chemotherapy and radiotherapy. Also it can improve survival rate, quality of life. However there isn't still a systemic clinical protocol for ovarian cancer in Korean medicine. This study will be helpful to establish clinical practice guidelines of Korean Medicine for ovarian cancer. And further studies on integrative ovarian cancer treatment are needed to build the clinical practice guidelines of ovarian cancer.