자바리, Epinephelus bruneus의 난 발생과 부화에 미치는 수온의 영향

양문호,최영웅,정민민,구학동,오봉세,문태석,이창훈,김경민,한석중,Yang, Moon-Ho,Choi, Young-Ung,Jung, Min-Min,Ku, Hag-Dong,Oh, Bong-Sae,Moon, Tae-Seok,Lee, Chang-Hoon,Kim, Kyong-Min,Han, Seock-Jung 한국발생생물학회 2007 발생과 생식 Vol.11 No.2

This study examined temperature effect in egg development and hatching of longtooth grouper, Epinephelus bruneus. Fertilized embryos was not growth after morula stage at $15^{\circ}C$, at 18, 21, 24 and $27^{\circ}C$, the required time from fertilized embryos to hatching were 70 h. 30 min., 44 h. 10 min., 29 h. 10 min. and 24 h. 30 min., respectively. The hatching rates at $24^{\circ}C$ were higher than the other conditions and the hatching was not occurred at $15^{\circ}C$. These results suggest that the water temperature range of egg development and hatching was $18{\sim}27^{\circ}C$. 이 연구는 실내 사육수조에서 자연산란 후 수정된 난을 대상으로 수온에 따른 난 발생속도와 부화율을 조사하였다. 부화에 이르기까지 각 수온조건에서 소요된 시간은 $15^{\circ}C$에서 상실기 이후 발생이 진행되지 않았고, 18, 21, 24, $27^{\circ}C$에서 각각 70시간 30분, 44시간 10분, 29시간 10분 그리고 24시간 30분이 소요되었다. 부화율은 $15^{\circ}C$에서 0%였고, 18, 21, 24 그리고 $27^{\circ}C$에서 각각 $8.3{\pm}1.2%$, $18.0{\pm}6.2%$, $24.0{\pm}4.0%$ 그리고 $17.0{\pm}7.2%$로 뚜렷한 차이 없이 $24^{\circ}C$에서 다소 높았고 21와 $27^{\circ}C$에서 비슷한 경향이었으며 $18^{\circ}C$에서 가장 낮게 나타났다. 이러한 결과에서 자바리의 난발생 수온범위는 $18{\sim}27^{\circ}C$로 제안된다.

2원 첨가 적색 OLED 소자의 발광특성

김경민,주성후,Kim, Kyong-Min,Ju, Sung-Hoo 한국전기전자재료학회 2007 전기전자재료학회논문지 Vol.20 No.6

To invest the luminescent characteristics of red light emitting OLED device, a dual dopant system was incorporated into the emitting layer. The multiple layer OLED device structure was $ITO(1500\;{\AA})/HIL(200\;{\AA})/a-NPD(600\;{\AA})/EML(300\;{\AA})/Alq_3(200\;{\AA})/LiF(7\;{\AA})/Al(1800\;{\AA})$. The concentrations of the rubrene dopant were tested at 0 vol.%, 3 vol.%, 6 vol.% and 9 vol.%. The maximum device efficiency and life time were obtained at the rubrene dopant concentration of 6 vol.%. Emission spectrum and color coordinate of devices showed no relationship with rubrene dopant concentration. Experiment results show that rubrene dopant absorbs energy from $Alq_3$ host and transfer it to RD1 dopant acting as an energy intermediate and influencing the device efficiency, finally the red light is emitted from the RD1 dopant.

Gluconacetobacter sp. gel_SEA623-2를 이용한 Bacterial Cellulose 생산에 초산농도 및 유산균 혼합배양이 미치는 영향

김경민,김지현,양경월,Kim, Kyung min,Kim, Jihyeon,Yang, Kyong Wol 한국미생물학회 2014 미생물학회지 Vol.50 No.3

In this study, Gluconacetobacter sp. gel_SEA623-2 isolated from citrus that produces bacterial cellulose was used to examine the effect of initial concentration of acetic acid and mixed culture inoculated with Lactobacillus plantarum KCCM 80077 on productivity of bacterial cellulose. In mixed culture added with 0.5% acetic acid, the viable cell count increased from $2.4{\times}10^6CFU/ml$ to $1.1{\times}10^7CFU/ml$ after 14 days of culture, and total acidity was about 0.3% higher than single culture added with 0.5% acetic acid, which implies that additional lactic acid was produced by L. plantarum KCCM 80077. In single culture, although bacterial cellulose productivity was higher when the initial concentrations of acetic acid were 0.0% and 0.5%, than when it was 1.0%, there was no significant difference. However, in mixed culture, adding 0.5% acetic acid resulted in dry weight of $37.83{\pm}6.81g/L$ and thickness of $10.33{\pm}0.58mm$, showing a significant difference from that of single culture added with 1% acetic acid, $28.40{\pm}1.23g/L$ and $7.50{\pm}0.50mm$ (P<0.05). 본 연구에서는 bacterial cellulose를 생산하는 감귤에서 분리된 Gluconacetobacter sp. gel_SEA623-2를 이용하여 초기 초산농도 및 Lactobacillus plantarum KCCM 80077 유산균을 접종한 mixed culture가 bacterial cellulose 생산성에 미치는 영향을 알아보았다. 0.5%의 초산을 첨가한 mixed culture의 경우 초기 생균수가 $2.4{\times}10^6CFU/ml$에서 14일 동안 배양한 이후에 $1.1{\times}10^7CFU/ml$까지 증가하였고 total acidity가 0.5%의 초산을 첨가한 single culture와 비교하여 0.3% 가량 높게 측정되어 유산균에 의해 추가적으로 젖산이 생성된 것으로 판단된다. Single culture에서는 초기 초산농도가 0.0 및 0.5%일 때 1.0% 농도와 비교하여 상대적으로 높은 bacterial cellulose 생산성을 나타내기는 하였으나 유의적인 차이는 보이지 못하였다. 하지만 mixed culture에서는 0.5%의 초산을 첨가하였을 때 $37.83{\pm}6.81g/L$의 dry weight과 $10.33{\pm}0.58mm$의 thickness를 나타냄으로써 1%의 초산을 첨가한 single culture의 $28.40{\pm}1.23g/L$와 $7.50{\pm}0.50mm$의 생산량과 비교하여 유의적인 차이를 나타내었다(P<0.05).

터널 버력처리 공정의 시뮬레이션 적용성에 관한 연구

김경민,서형범,황호정,김경주,Kim, Kyoung-Min,Seo, Hyeong-Beom,Hwang, Ho-Jung,Kim, Kyong-Ju 한국건설관리학회 2007 건설관리 : 한국건설관리학회 학회지 Vol.8 No.1

시뮬레이션은 건설관리를 위한 도구로써 많은 잠재력을 가지고 있으나, 국내의 경우 대단히 제한된 범위에서 마이크로 프로세스의 공정 평가에 적용을 시도하고 있는 단계에 있다. 시뮬레이션을 건설 공정에 적용하기 위해서는 현장 특성을 반영한 입력 데이터 수집과 모델 구축에 상당한 노력이 필요하다. 본 연구에서는 시뮬레이션 모델 구축을 위한 장비 운영 데이터 활용과 시뮬레이션 모델 구축 방법론을 소개하고, 현장 측정 데이터와의 검증을 수행하였다. 그리고 버력 처리 시스템의 전체 운행 주기(Cycle Times)를 결정하는 주요 요인을 분석하고 장비운영 계획을 수립하기 위한 시뮬레이션 활용 방안을 제시하였다. Simulation has considerable potential as the tool of construction management, but in the case of domestic, it is at an early stage applying simulation to micro-process assesment. For applying simulation to the construction process, much effort is needed to collect input data and to build the model including the characteristics of site. This study introduces the methodology to collect operation data of construction equipment and build the simulation model, then verifies the model with the operation data. In addition, this study identifies main factors to determine the cycle time of the muck hauling system so that it suggests the method of construction process planning through operation and combination of construction equipments.

고등어(Scomber japonicus ) 치어의 마취제로서 Clove oil, MS-222 및 2-Phenoxyethanol의 평가

한석중 ( Seock Jung Han ),김경민 ( Kyong Min Kim ),최낙중 ( Nack Jung Choi ),구준호 ( Jun Ho Koo ),박충국 ( Chung Kug Park ),이원교 ( Won Gyo Lee ),지승철 ( Seung Chul Ji ) 한국수산과학회(구 한국수산학회) 2011 한국수산과학회지 Vol.44 No.6

The efficiency of clove oil, MS-222, and 2-phenoxyethanol was evaluated as anesthetics in juvenile Scomber japonicus . Stage A5 of anesthesia was assumed to be sufficient for conducting routine aquaculture procedures in less than 3 min, with recovery (stage R5) in less than 5 min. The lowest effective doses of the three anesthetics were 50 mg L-1 clove oil (anesthetic time of 71.3 s and recovery time of 167.0 s), 100 mg L-1 MS-222 (anesthetic time of 70.7 s and recovery time of 115.7 s), and 400 mg L-1 2-phenoxyethanol (anesthetic time of 86.7 s and recovery time of 95.0 s). Anesthetic times decreased with increasing doses for all three anesthetic agents, and fish anesthetized with clove oil exhibited the longest recovery times. After 30 min, the highest plasma cortisol and lactate levels were detected with the use of clove oil, whereas the lowest values were observed with 2-phenoxyethanol. In addition, high glucose levels were maintained during recovery with clove oil, but the treatments did not significantly differ. The most effective of the three anesthetic agents was 2-phenoxyethanol, although all were considered acceptable for use in cultures of juvenile Scomber japonicus

사육조건에 따른 능성어, Epinephelus septemfasciatus, 수정란의 난질 및 아미노산 조성

김경민 ( Kyong Min Kim ),조재권 ( Jae Kwon Cho ),박종연 ( Jong Youn Park ),손맹현 ( Maeng Hyun Son ),박재민 ( Jae Min Park ),한경호 ( Kyeong Ho Han ),홍창기 ( Chang Gi Hong ) 한국어류학회 2016 韓國魚類學會誌 Vol.28 No.4

본 연구는 능성어 부상란과 침강란의 아미노산 분석을 통하여 난의 생존에 필요한 요소를 구명하고, 사육환경 및 먹이에 따른 난질의 변화를 난의 생화학적 분석을 통하여 난질에 영향을 미칠수 있는 요소를 구명하기 위하여 수행하였다. 그 결과 육상수조에서 사육하는 것보다 해상가두리에서 사육할 때 난질이 우수하였고, 서로 다른 먹이를 공급한 후 배란을 유도하여 난의 특징을 조사하였다. 그 결과 배합사료만 공급하거나 배합사료와 생사료를 혼합급이한 것보다는 생사료만을 급이한 어미에게서 우수한 수정란을 얻을 수 있었다. 그리고 부상란과 침강란의 아미노산을 분석한 결과 부상란에서 유리아미노산의 함량이 높았으며, 배합사료만 급이하거나 배합사료와 생사료를 혼합 급이한 경우 보다는 생사료만을 공급한 경우 수정란의 유리아미노산의 함량이 높았다. 즉, 난질이 우수할수록 유리아미노산의 함량이 높았다. This study aims to investigate egg quality and amino acid composition of buoyant and non-buoyant eggs and evaluate egg quality of sevenband grouper, Epinephelus septemfasciatus. Amino acid analysis of eggs was conducted to investigate what elements were necessary for the survival and good quality of egg depending on farming condition and different diet. We analyzed amino acid from buoyant eggs and non-buoyant eggs, farming conditions (tank and sea cage), and different dietary conditions (formulated feed, formulated feed+raw fish-based moist pellets, and raw fish-based moist pellets). Egg quality was the best in a sea cage and when raw fish-based moist pellets (MP) were fed. In addition, egg quality with formulated and MP was better than that with formulated feed. As a result of amino acid analysis of eggs, buoyant eggs were containing more free amino acid than non-buoyant eggs. Also, eggs with MP were containing more free amino acid than those with formulated feed and MP. Eggs with mixed formulated feed and MP were containing more free amino acid than formulated feed. In conclusion, amino acid was helpful to improve egg quality, and egg quality can be controlled by farming conditions and feed.

스마트폰뱅킹 도입에 따른 국내은행의 고객정보보호에 관한 연구

김경민(Kim Kyong Min) 한국정보보호학회 2010 情報保護學會誌 Vol.20 No.6

2009년 11월 KT의 애플사의 스마트폰인 아이폰(i-phone)의 국내 도입과 함께 촉발된 국내 스마트폰시장의 확산은 2010년 구글을 중심으로 삼성전자,SKT등 기존 대기업들의 갤럭시폰을 비롯한 안드로이드 OS기반의 스마트폰을 선보이면서 스마트폰 가입자가 2010년 말까지 약 600만명에 이를 것으로 전망된다. 이러한 스마트폰의 확대와 함께 2009년말 기업,하나은행 등 일부 은행들이 처음 스마트폰뱅킹을 시작한 이례 2010년 상반기, 이미 국내 대부분의 시중은행들이 아이폰용 iOS를 비롯한 주요 스마트폰의 OS 플랫폼을 기반으로 한 스마트폰뱅킹 서비스를 제공하고 있다. 그러나 금융산업의 특성상, 금융거래의 신뢰성과 안정성 측면에서의 개인정보보호 및 금융정보보안과 관련한 법규가 채 정비되지 않은 가운데 국내 은행들은 스마트폰뱅킹 출시경쟁에 치중한 나머지 보안 및 개인정보보호에 대한 대책수립은 미흡하며 정부당국의 정보보호관련 지침에 의존, 고객의 자발적인 주의에 호소하고 있는 실정이다. 이처럼 스마트폰뱅킹의 도입이 활발히 진행되면서 금융 서비스 혁신 등 기술적 편의성외에도 개인정보보호를 위한 보안 및 법적 안정성의 중요성이 높이지고 있다. 본 논문은 국내 은행의 스마트폰뱅킹 현황과 고객정보보호 및 관리에 대한 법률적 고찰과 규제환경 변화를 중심으로 국내 은행의 스마트폰뱅킹 도입에 따른 고객정보보호 방안을 제시하고자 한다.

IMO MEPC의 최근 3년간 대기오염 규제동항(MEPC 53rd~57th)

김경민(Kyong-Min Kim),이돈출(Don-Chool Lee),남정길(Jeong-Gil Nam) 한국마린엔지니어링학회 2008 한국마린엔지니어링학회 학술대회 논문집 Vol.2008 No.-

Today's top issues around the world are about the environmental problems; such as global warming, acid rain, ozone layer depletion, etc. In order to find the suitable solution, IMO MEPC has been doing it's best to prevent air pollution from ships since 1998. This paper summarizes the most recent trends in regulations that were discussed and announced during MEPC 53rd to 57th sessions (for 3years), and suggests what the Republic of Korea should do for both the international societies and our maritime industries in the near future.

폐흡충 피낭유충 표피의 형태학적 관찰

金京民(Kyong-Min Kim),安明姬(Myoung-Hee Ahn),閔得映(Duk-Young Min) 대한기생충학열대의학회 1987 The Korean Journal of Parasitology Vol.25 No.2

적립계 장수형 붉은곰팡이병 중도저항성 제면용 밀(Triticum aestivum L.) ‘태중’

김경민(Kyeong-Min Kim),김경훈(Kyeong-Hoon Kim),정영근(Young-Keun Cheong),최창현(Chang-Hyun Choi),김양길(Yang-Kil Kim),박종호(Jong-Ho Park),김경호(Kyong-Ho Kim),장지선(Jiseon Jang),최진경(Jin Kyung Choi),배정숙(Jeong-Suk Bae),민병규(B 한국육종학회 2019 한국육종학회지 Vol.51 No.4

A winter wheat (Triticum aestivum L.) cultivar, ‘Taejoong’, was developed by the National Institute of Crop Science, Rural Development Administration in 2016. It was derived from a cross between ‘Xian83(104).11’ and ‘Keumkang’ in 2005. It was generated through the bulk and pedigree methods for six years, and then designated as ‘Iksan370’ after a two-year advanced yield trial test. This variety was designated the name ‘Taejoong’ after a regional yield trial test in eight locations throughout Korea for three years from 2014 to 2016. Its heading date was April 27 in upland and paddy filed conditions, and its maturity date was June 7 in uplands and June 4 in paddy fields, which were late compared to that of ‘Keumkang’. ‘Taejoong’ had 417 spikes per m2, which was 300 less than that of ‘Keumkang’. However, the spike length was 13.4 cm and the number of kernel per one spike was 48, which was 5.8 cm longer and 19 higher than those of ‘Keumkang’, respectively. ‘Taejoong’ showed strong resistance to lodging and moderate resistance to Fusarium head blight, but was susceptible to powdery mildew. ‘Taejoong’ flour yield (71.8%) and flour lightness (91.90) were similar to those of ‘Keumkang’, but its protein content (11.1%), gluten content (8.8%), and sodium dodecyl sulfate-sedimentation volume (34.2 ml) were lower. These result showed that the flour dough strength of ‘Taejoong’ was weaker than that of ‘Keumkang’. The high-molecular-weight gluten subunit compositions of ‘Taejoong’ were Glu-A1 (N), Glu-B1 (7+9), and Glu-D1 (2+12). The granule-bound starch synthase compositions were Wx-A1, Wx-B1, and Wx-D1. The puroindoline compositions were Pina-D1 (a type) and Pinb-D1 (b type). The average grain yield of ‘Taejoong’ in a regional yield trial was 5.3 ton/ha in uplands and 4.6 ton/ha in paddy fields, which were 21% and 13% higher than that of the reference cultivar, ‘Keumkang’, respectively. (Registration No. 7378).