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- 저자 : 길기정 ( Ki-jung Kil ) (검색결과 26 건)
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길기정 ( Ki-jung Kil ),도은수 ( Eun-soo Doh ),장준복 ( Jun-pok Chang ),이승현 ( Seung-hyun Lee ),유지현 ( Ji-hyun Yoo ) 대한본초학회 2015 大韓本草學會誌 Vol.30 No.4
Objectives: The objective of this research was to investigate the antibacterial and antioxidant activities of bio-fermented Sanguisorbae Radix extract. Methods: The Sanguisorbae Radix extract was fermented by Streptococcus thermophilus, and their products was tested for antibacterial activity against pathogenic microorganisms namely, Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Vibrio parahaemolyticus and Salmonella typhimurium by paper disc diffusion method and the antioxidant activities of extract was evaluated by five different assays as electron donating ability(EDA), superoxide dismutase(SOD)-like activity, polyphenol, flavonoid contents and nitrite scavenging ability. Results: The bio-fermented Sanguisorbae Radix extract was safe from heat. Antibacterial activity of fermented Sanguisorbae Radix extract appeared relatively highly against Bacillus cereus and Staphylococcus aureus and didn``t show any difference. EDA in comparison to Vitamin C showed over 90% activity at about the same time of Sanguisorbae Radix extract expressed highly. SOD activity showed 15% in fermentation before and after. The nitrite scavenging ability of Sanguisorbae Radix extract before and after fermentation showed higher numerical value over 70% in pH 2.5 than that of butylated hydroxytoluene(BHT). But SOD activity, EDA and nitrite scavenging ability were not different between the Sanguisorbae Radix extract before and after fermentation. Total polyphenol content expressed over about 20 mg/g, and that of the Sanguisorbae Radix extracts was increased than that of the fermented Sanguisorbae Radix extracts. Conclusions: The results suggest the usefulness of developing functional materials using antioxidant active Sanguisorbae Radix extract was fermented by Salmonella typhimurium with high polyphenol contents and nitrite scavenging ability.
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상백피에 의한 MC/9 비만세포의 활성 억제 조절 연구
이기전 ( Ki Jeon Lee ),김복규 ( Bok Kyu Kim ),길기정 ( Ki Jung Kil ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.1
Objective : Morus alba Linne Root Bark (MRAL) is a medicinal herb in Korean Medicine, known for its anti-inflammatory and anti-allergic properties. However, its mechanisms of action and the cellular targets have not yet been found and the study was developed to investigate the allergic suppressive effect of MRAL. The purpose of this study is to investigate the allergic suppressive effects of MRAL on activation of MC/9 mast cells, Methods : Cytotoxic activity of MRAL (50, 100, 200, 400 μg/mL) on MC/9 mast cells measured using EZ-Cytox cell viability assay kit (WST reagent). The levels of interleukin-5 (IL-5), IL-13 and IL-4, IL-5, IL-6, IL-13 mRNA expression were measured by enzyme-linked immunosorbent assay (ELISA) and real-time PCR respectively. The expression of transcription factors such as GATA-1, GATA-2, NFAT, AP-1 and NF-κB p65 DNA binding activity were measured by western blot and electrophoresis mobility shift assay (EMSA), Results : Our results indicated that MRAL (50 μg/mL, 100 μg/mL) significantly inhibited PMA/Ionomycin-induced production of IL-5 and IL-13 and the expression of IL-4, IL-5, IL-6 and IL-13 mRNA in MC/9 mast cells. Moreover, MRAL (50 μg/mL, 100 μg/mL) inhibited PMA/Ionomycin-induced GATA-1, GATA-2, NFAT-1, NFAT-2, c-Fos protein expression and NF-κB p65 DNA binding activity in MC/9 mast cells, Conclusions : In conclusion, we suspect the anti-allergenic activities of MRAL, may be related to the regulation of transcription factors GATA-1, GATA-2, NFAT-1, NFAT-2, c-Fos and NF-κB p65 DNA binding assay causing inhibition of Th2 cytokines IL-5 and IL-13 in mast cells.
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LPS로 유도된 마우스 대식세포주인 RAW264.7에서 MAPK 조절에 의한 백미 물추출물의 항염증 활성
이상호,유지현,길기정,Lee, Sang-Ho,Yoo, Ji-Hyun,Kil, Ki-Jung 대한본초학회 2022 大韓本草學會誌 Vol.37 No.6
Objectives : To develop natural ingredients that help prevent or treat anti-inflammatory-related diseases and use themas basic data, we investigated anti-inflammatory activity of Cynanchi Atrati Radix Et Rhizoma water extracts(CWE) in lipopolysaccharide(LPS)-induced murine macrophage cell line, RAW 264.7 cells. Methods : The cell viabilities were evaluated with RAW 264.7 cells. The production of nitric oxide(NO), prostaglandin E2(PGE2), pro-inflammatory cytokines such tumor necrotic factor(TNF)-α and interleukin(IL)-6 were assessed in LPS-induced RAW 264.7 cell treated with CWE. Furthermore, the protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2(COX-2), and mitogen-activated protein kinase(MAPK) were assessed by western blotting. Results : In RAW 264.7 cell, the cell viability by CWE treatment was more than 98.4% at a concentration of 100-400 ㎍/mL. At a concentration of 800 ug/ml of CWE, the cell viability was as low as 86%. At doses of 100, 200 and 400 ㎍/mL, CWE inhibited the production of NO, PGE2, TNF-𝛼 and IL-6 in a dose-dependent manner and also decreased the expression of iNOS and COX-2 from LPS-induced RAW 264.7 cells. In addition, CWE significantly inhibited the MAPK pathway including decreased the phosphorylation of the p38, c-Jun N-terminal kinase(JNK) and extracellular signal-regulated kinase(ERK1/2). Conclusions : Our study provides evidence that CWE inhibits the production of main pro-inflammatory molecules in LPS-induced RAW 264.7 cells via expression of p38, JNK, and ERK1/2 MAPK signaling pathways. Therefore, CWE is expected to be widely used as a natural ingredient for anti-inflammatory functional foods or pharmaceuticals in the future.
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은행잎의 주성분인 bilobalide가 염증반응에 미치는 효과
정제룡 ( Je Ryong Jung ),길기정 ( Ki Jung Kil ) 대한본초학회 2015 大韓本草學會誌 Vol.30 No.1
Objectives : Bilobalide (BIL) is a predominant sesquiterpene trilactone constituent that accounts for a partial portion of the standardized Ginkgonis Folium extract, which has been widely used to treat a variety of neurological disorders involving cerebral ischemia and neurodegeneration. In this study, it was tested whether BIL exhibits anti-inflammatory activities on inflammation response, or not. Methods : To elucidate the molecular mechanisms of BIL on pharmacological and biochemical actions in inflammation, we examined the effect of BIL on pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated macrophages. The investigation was focused on how BIL affect on inflammation-related mediators including various signals such as nitric oxide (NO), prostaglandin E2 (PGE2), inducible NO synthase(iNOS), cyclooxygenase-2(COX-2), interleukin-6(IL-6), tumor necrosis factor-α (TNF-α), mitogen-activated protein kinases(MAPKs) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) in LPS-stimulated RAW 264.7 cells. Results : We found that BIL inhibited LPS-induced NO, PGE2, IL-6 and TNF-α productions as well as the expressions of iNOS and COX-2. Furthermore, BIL suppressed the LPS-induced phosphorylation for MAPK activation. Conclusions : These results suggest that BIL has inhibitory effects on LPS-induced PGE2, NO, IL-6 and TNF-α production, as well as the expressions of iNOS and COX-2 in the murine macrophage. It seems that these inhibitory effects occur by blocking the phosphorylation of MAPKs for activation. Then, BIL suppressed the activation of nuclear factor NF-кB in nucleus. These observations suggest that BIL has anti-inflammatory effect by inhibiting.
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양회정 ( Hwoi Jeong Yang ),길기정 ( Ki Jung Kil ),이영종 ( Young Jong Lee ) 대한본초학회 2004 大韓本草學會誌 Vol.19 No.2
N/A Objective : In order to reveal the morphological characteristics of Bupleuri Radix prevailed in Korea, the raw and artificial Bupleurum falcatum L. and the raw B. longiradiatum Turcz. were collected., and their external, internal, and flour states were observed via microscopic examination. Method : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and the flour of it was mounted by the liquid made by the same ratio of each of glycerin, acetic acid, and water, and then observed and photographed by olymphus -BHT. Result 1. The external form of B. falaztum L., plant root, looked like a circular cone and had no room between the joints. However, B. longiradiatum Turcz., plant rhizome, had well-articulated joints where many small roots were attached with a cavity in the middle of pith of its slice. 2. The internal form of B. falcatum L. has cork layers composed of 7~8 lines of cells, and 6~8 secretory cells were found around vitta. The cork layers of B. longiradiatum Turcz. was composed of over 10 lines of cells, and 10~20 secretory cells were distributed around vitta. 3. In the flour form, B. longiradiatum Turcz. contains rhizome epidermal cell, which was not observed in B. falcatum L.. Moreover, stoma was not consistently arranged. Conclusion There were differences in external, internal, and flour states between B. falcatum L. and B. longiradiatum Turcz., so that microscope can be used to distinguish between these two.
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진주환 ( Ju Hwan Jin ),길기정 ( Ki Jung Kil ),이영종 ( Young Jong Lee ) 대한본초학회 2004 大韓本草學會誌 Vol.19 No.2
N/A Objective : Allii Tuberosi Semen and Allii Fistulosi Semen have been sold mixed with fabricated forms of them in Korea. hn such a situation, seeds of Allium tuberosum Rottler, A. fistulosum Linne, and A. cepa Linne were collected and their external, internal, and flour states were observed through microscopic examination. Method : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and the flour of it was mounted by the liquid made by the same ratio of each of glycerin, acetic acid, and water, and then observed and photographed by olymphus-BHT. Result 1. Allii Tuberosi Semen showed the salient network-like wrinkles on its upper side, but Allii Fistulosi Semen and Allii Cepae Semen had 1 -2 lines on their upper sides. 2. The length of Allii Cepae Semen was 2.5?3.5mm, and the width of it was 1.5--2.5mm, which was a bit small; but the length of Allii Fistulosi Semen was 3-4mm, and the width of it was 2?3mm, which was a bit large. 3. In the flour forms, Allii Tuberosi Semen has network-like pattern in the surface of the seed coat epidermal cell wall, but Allii Fistulosi Semen and Allii Cepae Semen have litte or no such pattern. Conclusion : Because there are some clear differences in external and flour states of Allii Tuberosi Semen. Allii Fistulosi Semen, and Allii Cepae Semen, microscopic examination can be used to distinghish them and their fake forms in flour states.
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