임상원저 : 구개열 언어에 대한 Screening test 와 설문조사의 비교분석

권종진(Jong Jin Kwon),장현석(Hyun Seok Jang),박영준(Young Joon Park),최철민(Cheol Min Choi),전상호(Sang Ho Jeon),장수정(Su Jeong Jang),임재석(Jae Suk Rim) 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.6

Purpose : This study was carried out to evaluate the abnormalites of the speeches of the cleft patients and compare the results of the speech evaluations of a speech pathologist with those of parents. Materials and Methods: Eleven cleft patients` parents were questioned about their children`s speech and then the speeches of the cleft patients were evaluated by a speech pathologist and their results were compared with those of parents. Results: Nasal emission was noted in twenty percent of patients, with thirty percent demonstrating hypernasality and all demonstrating no hyponasality and aspirate. And twenty percent of patients show hoarseness. There was no statistically significant difference in the sensitivities to nasal emission between the speech pathologist and parents (p>0.05). However the speech pathologist is more sensitive to hypernasality than parents(p<0.05).

骨折部位 骨形成에 關한 實驗的 硏究

權鍾瑨(Kwon Jong Jin),金圭植(Kim Kyoo Sik) 대한치과의사협회 1981 대한치과의사협회지 Vol.19 No.4

혈소판 유래 성장인자가 임플란트 주위 골형성에 미치는 효과에 대한 실험적 연구

류재준,권종진,김한겸,Ryu, Jae-Jun,Kwon, Jong-Jin,Kim, Han-Kyum 대한치과보철학회 1998 대한치과보철학회지 Vol.36 No.6

Bony fixation of implants during the early phase of healing is important in order to get secondary stability of the implant assuring the success of the treatment. Because the successful placement of the implant is limited by the quality and quantity of bone, other agents which stimulate bone formation in the peri-implant spaces has been illustrated. Platelet-derived growth factor (PDGF) has been shown to regulate DNA and protein synthesis in bone cells in vitro and to interact synergistically to enhance soft tissue wound healing in vivo. The purpose of this study was to evaluate bone promotion around implants which were augmented with sagittal split osteotomy or autogenous veneer bone graft using the platelet derived growth factor(PDGF). After placement of newly designed twenty four screw-type implants, which were 12mm in length and 4mm in diameter in 6 dogs. $4{\mu}g$ of PDGF B/B was applied with surgicel carriers. The dogs were sacrificed at 3 days, 1, 2, 3, 6, and 12 weeks after implantation. Specimens were examined clinically, radiographically, histologically, and histomorphometrically. The results were as follows: 1. Clinically and radiologically, there was no significant difference in bone formation and healing pattern between experimental and control group. 2. In autogenous veneer bone graft group, bone formation was observed at 1st week in the experimental groups but 2nd week in the control groups. At 3rd week, the expeimental groups showed more bone formation comparing to the control groups. 3. In sagittal split osteotomy group, bone formation was observed at 1st week in both groups. But the experimental groups showed more bone formation comparing to the control groups after 2nd week. 4. The bone growth rate of experimental group was more rapid than that of control group. These results indicated that PDGF did not affect the initiation of new bone formation, but it accelerated the bone formation at the early period.

POSTOPERATIVE TOXIC SHOCK SYNDROME: REPORT OF A CASE

장현석,권종진,임재석,이갑재,Jang, Hyon-Seok,Kwon, Jong-Jin,Lim, Jae-Seok,Lee, Kab-Jae Korean Association of Maxillofacial Plastic and Re 1999 Maxillofacial Plastic Reconstructive Surgery Vol.21 No.1

Toxic Shock Syndrome(TSS)은 1978년 Todd등에 의해 처음 보고된 이래 젊은 여성에서 월경과 연관된 질환으로 생각되어 왔으나 최근에는 코수술을 비롯한 두경부수술 및 기타 소수술후 합병증으로 많은 증례가 발표되고 있다. 이의 진단은 주로 임상적 진단, 즉 갑작스런 술후 고열, 설서, 저혈압 및 홍피증과 혈액배양등에서 검출되는 포도상구균등으로 가능하다. TSS은 대부분 수액공급 및 혈압의 유지, 전신적인 항생제 투여등으로 쉽게 치료가 가능하지만, 경과가 좋지 않은 경우 면역글로불린의 사용도 시도되어 좋은 결과를 얻고 있으며 조기 발견 및 처치가 가장 중요하다고 할 수 있다. 이에 저자는 상악골 및 비골, 안와근심벽 골절을 지닌 48세 남자환자에서 골절부에 대한 관혈적 정복고정술후 발생된 TSS의 처치에 대해 문헌고찰과 함께 보고하는 바이다. Toxic shock syndrome is an acute disease involving multiple organs. I described here a case of TSS associated with intranasal packing. Four major criteria, which are involvement of multiple organ systems, fever, hypotension or shock, and rash with subsequent desquamation should be fulfilled for the diagnosis. The exact pathogenesis is not well understood, however it is thought to be due to the effects of an enterotoxin produced by certain strains of staphylococcus aureus.

삼차원 유한요소 해석에 의한 골내 임프란트의 구조에 관한 연구

현연근,권종진,Hyun, Young-Keun,Kwon, Jong-Jin 대한치과보철학회 1997 대한치과보철학회지 Vol.35 No.1

The stress distribution generated in the surrounding bone was calculated and compared for various geometry of the dental implants by means of the finite element methods. The models were designed to represent the screw type endosseous implants(varing the size, shape, direction of the screw thread and the angle of the body) with supporting bone and the cylinder type endosseous implants(varing the lower portion-Round type, tapered type) with supporting bone. Static mean bite forces were applied 100N vertically and 25N horizontally on the center of the implant and three dimensional finite analysis was undertaken using software ANSYS 5.1 Version. The result demonstrated that different implant shape leads to significant variations in stress distribution in the bone. In the case of variation of the screw size, direction and shape the implant model with normally directional and triangular screw implied lower stress than with upper directional or lower directional and quadrangular screw but among models a different screw size, within a variation of 0.2mm there was no meaningful difference in maximum stress. In the case of variation of angle of body the straight implied lower stress than the tapered. As a result of analysis of cylinder type, the implants with larger radius of curvature of the round form and larger diameter of the tapered form implied lower stress.

임프란트로 지지된 하악 켄티레버 보철물의 3차원 유한요소 분석

조철,신상완,권종진,Cho, Chul,Shin, Sang-Wan,Kwon, Jong-Jin 대한치과보철학회 2000 대한치과보철학회지 Vol.38 No.5

One of the biggest clinical problems of osseointegrated implant prosthesis is the excessive stress caused by bite forces which are transfered directly into the bone through the osseointegrated implant fixtures. So several biodynamic problems occur when there is an excessive fatigue stress. The factors of stress distribution are the number, kind, position, arrangement of the implants, and the distance between the implants, and the kind, quality of superstructure prosthesis and connection type between the rest implant and the superstructure. Recently, a distal short additional implant, socalled rest implant, is employed to reduced the stresses in conventional cantilevered prostheses. This study was undertaken to analyze the stresses transfered by osseointegrated implant cantilevered prostheses depending upon the number and the position of implants, the presence of rest implant, and the type of their connection. Three dimensional finite element analysis was attempted using ANSIS ver. 5.3 program under IBM INDIGO computer. The results were as follows : 1. The rest implant influenced on the pattern of stress distribution on the anterior area of the mandible and the superstructure. 2. In the group employing the rest implants, the fixed type of connection between the rest implant and the superstructure was more stable than the ball attachment type on the stress distribution. 3. In the group employing the ball attachment between the rest implant and the superstructure, the case with 4-implants(on canine, premolar) was little more stable than the case with 6-implants and the case with 4-implants(on incisor, premolar) on the stress distribution. 4. In the cantilevered group, the case with 4-implants(on incisor, premolar) and the case with 6-implants were more stable than the case with 4-implants(on canine, premolar) on the stress distribution. 5. In all of the group, the case with 6-implants and the fixed type of connection was the most stable and the case with 4-implants (on canine, premolar) was the most unstable on the stress distribution.

HSP27 EXPRESSION IN OSTEOBLAST BY THERMAL STRESS

임재석,김병렬,권종진,장현석,이의석,전상호,우현일,Rim, Jae-Suk,Kim, Byeong-Ryol,Kwon, Jong-Jin,Jang, Hyon-Seok,Lee, Eui-Suk,Jun, Sang-Ho,Woo, Hyeon-Il Korean Association of Maxillofacial Plastic and Re 2008 Maxillofacial Plastic Reconstructive Surgery Vol.30 No.1

Aim of the study: Thermal stress is a central determinant of osseous surgical outcomes. Interestingly, the temperatures measured during endosseous surgeries coincide with the temperatures that elicit the heat shock response of mammalian cells. The heat shock response is a coordinated biochemical response that helps to protect cells from stresses of various forms. Several protective proteins, termed heat shock proteins (hsp) are produced as part of this response. To begin to understand the role of the stress response of osteoblasts during surgical manipulation of bone, the heat shock protein response was evaluated in osteoblastic cells. Materials & methods: With primary cell culture studies and ROS 17/2.8 osteoblastic cells transfected with hsp27 encoding vectors culture studies, the thermal stress response of mammalian osteoblastic cells was evaluated by immunohistochemistry and western blot analysis. Results: Immunocytochemistry indicated that hsp27 was present in unstressed osteoblastic cells, but not fibroblastic cells. Primarily cultured osteoblasts and fibroblasts expressed the major hsp in response to thermal stress, however, the small Mr hsp, hsp27 was shown to be a constitutive product only in osteoblasts. Creation of stable transformed osteoblastic cells expressing abundant hsp27 protein was used to demonstrate that hsp27 confers stress resistance to osteoblastic cells. Conclusions: The demonstrable presence and function of hsp27 in cultured bones and cells implicates this protein as a determinant of osteoblastic cell fate in vivo.

지방조직 유래 줄기세포의 조골세포로의 분화에 대한 실험적 연구

이의석,장현석,권종진,임재석,Lee, Eui-Seok,Jang, Hyon-Seok,Kwon, Jong-Jin,Rim, Jae-Suk 대한악안면성형재건외과학회 2008 Maxillofacial Plastic Reconstructive Surgery Vol.30 No.2

Stem cells have self-renewal capacity, long-term viability, and multiline age potential. Adult bone marrow contains mesenchymal stem cells. Bone marrow-derived mesenchymal stem cells (BMSCs) are progenitors of skeletal tissue components and can differentiate into adipocytes, chondrocytes, osteoblasts, and myoblasts in vitro and undergo differentiation in vivo. However, the clinical use of BMSCs has presented problems, including pain, morbidity, and low cell number upon harvest. Recent studies have identified a putative stem cell population within the adipose tissue. Human adipose tissue contains pluripotent stem cells simillar to bone marrow-derived stem cells that can differentiate toward the osteogenic, adipogenic, myogenic, and chondrogenic lineages. Human adipose tissue-derived stem cells (ATSCs) could be proposed as an alternative source of adult bone marrow stem cells, and could be obtained in large quantities, under local anesthesia, with minimal discomfort. Human adipose tissue obtained by liposuction was processed to obtain ATSCs. In this study, we compared the osteogenic differentiation of ATSCs in a specific osteogenic induction medium with that in a non-osteogenic medium. ATSCs were incubated in an osteogenic medium for 28 days to induce osteogenesis respectively. Osteogenic differentiation was assessed by von Kossa and alkaline phosphatase staining. Expression of osteocyte specific bone sialoprotein, osteocalcin, collagen type I and alkaline phosphatase, bone morphogenic protein 2, bone morphogenic protein 6 was confirmed by RT-PCR. ATSCs incubated in the osteogenic medium were stained positively for von Kossa and alkaline phosphatase staining. Expression of osteocyte specific genes was also detected. Since this cell population can be easily identified through fluorescence microscopy, it may be an ideal source of ATSCs for further experiments on stem cell biology and tissue engineering. The present results show that ADSCs have an ability to differentiate into osteoblasts. In the present study, we extend this approach to characterize adipose tissue-derived stem cells.

PLEIOTROPHIN (PTN) EXPRESSION IN OSTEOBLASTIC CELLS

김병렬,임재석,권종진,장현석,이의석,전상호,김영진,Kim, Byeong-Yol,Rim, Jae-Suk,Kwon, Jong-Jin,Jang, Hyon-Seok,Lee, Eui-Seok,Jun, Sang-Ho,Kim, Young-Jin Korean Association of Maxillofacial Plastic and Re 2007 Maxillofacial Plastic Reconstructive Surgery Vol.29 No.6

Pleiotrophin or osteoblast-specific factor 1(HOSF-1) is a growth-associated protein present in bone matrix. This study was designed to study pleiotrophin expression in osteoblastic cells. Pleiotrophin was expressed by osteoblast-like cell line. Pleiotrophin expression increased following the proliferative phase and was minimal at the terminal phases of the induced differentiation of cultured MC3T3-E1 cells. Pleiotrophin expression represents another autocrine factor that may contribute to the physiologic control of induced bone formation. In this study, induced osteogenesis will be examined in the context of the osteoblast expression of and regulation by PTN. I hypothesized that PDGF-BB stimulation of PTN expression represents an important paracrine signal during the induced osteogenesis associated with periodontal and implant surgeries. The possible mediation by PTN of anabolic effects attributed to PDGF-BB stimulation was examined in cell culture models of osteoblast differentiation. These studies will contribute fundamental insights to osteoblast biology and insights regarding the potential use of factors such as PTN in the clinical environment.

Acidic fibroblast growth factor가 신경재생에 미치는 영향에 관한 실험적 연구

최성원(Sung Weon Choi),권종진(Jong Jin Kwon) 대한구강악안면외과학회 1997 대한구강악안면외과학회지 Vol.23 No.2

Fibroblast growth factor (FGF) is a well-known angiogenic factor and mitogen for endothelial cells, fibroblasts, and Schwann cells. An experimental study on the sciatic nerve of rat was performed to evaluate the effect and to determine the optimal concentration of exogenously-applied aFGF on nerve regeneration. Sixty adult male Sprague-Dawley rats weighing between 200 and 250 gm were divided into control group and 3 experimental groups. The experimental groups were further divided into 0.25 ug/ml, 2.5 ug/ml, and 25 ug/ml aFGF group. 10 mm segmental defect was made at right sciatic nerve and both nerve ends was connected with the polyethylene tube into which aFGF-collagen solution was instilled. After 2, 4, 8 weeks, control and 3 experimental groups were examined by clinical appearance, electrophysiological assessment, and morphologic findings using light microscope, electron microscope, and image analyzer at mid-chamber cross section. The results were as follows. 1. Nerve regeneration was seen in Group 2 (2.5 ug/ml aFGF) and Group 3 (25 ug/ml aFGF) at 2 weeks and the diameter of middle regeneration part of 3 experimental groups was larger than that of control group (p<0.05). 2. There were little differences in latency and amplitude among each group (p>0.05). 3. At 4 weeks, the diameter of myelinated nerve fiber and the thickness of myelin of 3 experimental groups at 4 weeks were larger than those of control group (p<0.05). At 8 weeks, the diameter of myelinated nerve fiber and the thickness of myelin of Group 2 (2.5 ug/ml aFGF) were larger than those of other experimental groups (p<0.05). These results suggest that aFGF enhance peripheral nerve regeneration and nerve regeneration of 2.5 ug/ml aFGF group (Group 2) is more effective than that of any other groups. But, the high concentration of aFGF may not necessarily enhance nerve regeneration. We conclude that it is necessary to examine further long term study for determining optimal concentration of aFGF.