양혈거풍탕의 효능에 관한 실험적 연구 ( An Experimental Study on the Effect of Yanghyulguopoongtang )

권정남(Jung Nam Kwon),조기호(Ki Ho Cho),김영석(Young Suk Kim),배형섭(Hyoung Sup Bai),이경섭(Kyung Sup Lee) 경희대학교 경희의료원 1990 慶熙醫學 Vol.6 No.4

배양된 흰쥐 담관 섬유모세포에 대한 간흡충 분비배설 물질의 영향

권정남,민병훈,이행숙,김수진,주경환,Kwon, Jung-Nam,Min, Byoung-Hoon,Lee, Haeng-Sook,Kim, Soo-Jin,Joo, Kyoung-Hwan 한국현미경학회 2009 Applied microscopy Vol.39 No.2

간흡충(Clonorchis sinensis)은 우리나라에서 높은 감염률(2.9%)을 나타내는 기생충으로(KAHP, 2004), 간흡충에 감염된 담관은 간흡충의 흡반에 의한 물리적 자극과 대사산물 및 분비물 등의 화학적 자극에 의해 담관염이 일어나고, 간흡충이 성장할 때 충체 주위의 담관 상피세포의 증식, 탈락, 담관 주위의 염증 및 섬유화가 일어난다. 담관 점막에 분포하는 섬유모세포는 결합조직을 구성하는 세포의 한 종류로서 세포질돌기들이 잘 발달된 형태적 특징이 있으며, 세포질 내에 세포의 형태 유지, 신호전달, 인접세포와의 연접 등에 관여한다. 또한 조직을 발달시키고, 조직이 손상된 부위에서 콜라겐 층을 형성하여 손상된 조직이 복구되도록 하기도 한다. 상처의 반흔(scar) 형성과 지방축적, 염증(inflammation) 발생 과정에서 섬유모세포의 작용이 제대로 조절되지 못하면 섬유증(fibrosis)이 발달하게 된다는 연구 보고도 있다. 본 연구에서는 정상 흰쥐에서 분리된 담관 섬유모세포와 간흡충 감염 흰쥐에서 분리된 담관 섬유모세포를 배양하고, 각각의 실험군에 간흡충 분비배설 물질(Cs excretory-secretory product, ESP)을 첨가하여 배양하였다. 배양된 섬유모세포의 미세구조 변화와 세포 표면에 존재하는 sialic acid 및 actin의 분포를 전자현미경으로 관찰하여, 간흡충 감염에 따른 섬유모세포의 변화 및 간흡충 분비배설 물질의 자극에 따른 섬유모세포의 변화를 관찰하여 흰쥐의 담관 섬유모세포와 간흡충 감염과의 연관성을 알아보고자 하였다. 정상 흰쥐 담관에서 분리한 섬유모세포(G1)에 비하여 간흡충에 감염된 흰쥐 담관에서 분리한 섬유모세포(G2)와 간흡충 분비배설 물질을 첨가하여 배양한 섬유모세포(G1-1, G2-1)의 증식속도가 느린 것이 확인되었다. 세포질돌기의 수는 간흡충에 감염된 흰쥐의 담관으로부터 분리된 섬유모세포(G2)에서 가장 많은 수가 관찰되었고, 배양배지에 분비배설 물질을 첨가하면 정상 담관의 섬유모세포에서도 세포질돌기가 증가하였다. 따라서 간흡충 대사물질은 섬유모세포의 세포질돌기형성을 촉진시키는 것으로 생각된다. 간흡충에 감염된 흰쥐 담관에서 분리한 섬유모세포(G2)의 소포체는 정상 담관에서 분리된 섬유모세포(G1)의 것에 비하여 감소하는 양상을 나타내었고, 여기에 분비배설 물질을 첨가하면 섬유모세포의 소포체가 증가하는 것이 관찰되었다. 그리고 세포표면에 분비되는 sialic acid는 주로 세포질의 소포낭 주변에서 관찰되었으며, 정상 섬유모세포(G1, G1-1)보다 감염된 섬유모세포(G2, G2-1)에서 증가하였다. Actin은 세포표면과 세포질돌기에서 주로 관찰되었으며, 정상 섬유모세포(G1, G1-1)보다 감염된 섬유모세포(G2, G2-1)에서 반응이 증가하였고, 간흡충 분비배설 물질을 첨가하면 G1-1은 반응이 증가하고, G2-1은 반응이 감소하는 것이 관찰되었다. 이상의 결과로 간흡충에 감염된 흰쥐 담관의 섬유모세포는 세포질돌기들이 매우 발달하며, actin단백과 sialic acid가 증가하여 세포변형을 초래하게 된다. 또한 간흡충 감염으로 손상된 담관의 섬유모세포로 구성된 결합조직은 정상으로 회복되지 않으며, 세포질 부피 및 세포질돌기의 증가는 이루어지지만 간흡충 대사물질의 영향으로 섬유모세포의 분열 및 성장 속도가 억제되는 것으로 확인되었다. 이 결과 간흡충 감염으로 손상된 숙주의 담관 결합조직과 섬유모세포들은 간흡충 대사물질에 의하여 변형을 일으키고, 세포 활성 및 증식이 Clonorchis sinensis is the most important widely distributed parasite of the human bile duct in East Asia and the most prevalent parasitic helminth in Korea. The prevalence rate of human clonorchiasis has remained at about 2.9% in Korea. C. sinensis induces dilatation of the duct, hyperplasia of the mucosa, metaplasia or neoplasia of the mucosal epithelium, periductal inflammation and fibrosis, and thickening of the ductal wall. Fibroblast are the most common cells in connective tissue and are responsible for the synthesis of extracellular matrix components. The fibrosis associated with chronic inflammation and injury may also contribute to cholangiocarcinoma pathogenesis, particularly through an increase in extracellular matrix components, which participate in the regulation of bile duct differentiation during development. In this study, ultrastructural changes, the distribution of lectin receptors and actin protein in cultured SD rat bile duct fibroblast after infection of C. sinensis were observed. Experimental group had been divided into four groups: normal bile duct fibroblast cultured in basal media (G1); C. sinensis infected bile duct fibroblast cultured in basal media (G2); normal bile duct fibroblast cultured in basal media containing excretory-secretory product (ESP) (G1-1); C. sinensis infected bile duct fibroblast cultured in basal media containing ESP (G2-1). Overall, once a host is infected by C. sinensis, it affects the host to the extent that sialic acid of ductal fibroblast is increased. Number of cytoplasmic process of SD rat bile duct fibroblast was increased. Actin protein and sialic acid were located in cell surface. Fibroblast induced by C. sinensis was not recovered to normal fibroblast. The cytoplasm bulk and cytoplasmic process were increased whereas the growth rate of the fibroblast of infected SD rat was reduced rather than that of normal fibroblast. In result, it inhibits fibroblast proliferation and increases actin protein on fibroblast cytoplasm, and so causes fibroblast metamorphosis and cellular mutation.

중풍 환자의 기능 회복에 관한 임상적 고찰

권정남,김영균,조은희,Kwon, Jung-Nam,Kim, Young-Kyun,Cho, Eun-Hee 대한한방내과학회 2001 大韓韓方內科學會誌 Vol.22 No.4

In the oriental medicine field, researches have been in progress regarding oriental medical factors. I also carried out a clinical study on stroke, from which I discovered meaningful results about important factors that impact on the prognosis of stroke. We studied 132 patients after diagnosis of stroke through a Brain-CT scan and MRI sea, to the oriental internal medicine department at the Hospital affiliated to Oriental Medical College, Dongeui University. All the patients showed meaningful improvement in the examination of their symptoms after four weeks, compared with the first week. Between strokes involving meridian system and strokes involving internal organs, the symptom of paralysis caused by strokes involving meridian system, which patients were in favorable initial condition, had improved significantly and that by strokes involving internal organs had not. The group whose initial bowel movement since the stroke was delayed, whose coating on the tongue was thick or the tongue was dry, whose pulse was tachycardiac, or who had a history of hypertension or diabetics showed a significantly worse symptom after 4 weeks than that of groups in the opposite cases.

침습, 비침습 혈관레이저에 의한 고콜레스테롤혈증의 치료 효과 환자 유형별 비교

권미정,김민규,허성웅,윤현민,김원일,권정남,김영균,Kwon, Mi-Jung,Kim, Min-Kyu,Heo, Sung-Woong,Youn, Hyoun-Min,Kim, Won-Il,Kwon, Jung-Nam,Kim, Young-Kyun 대한약침학회 2006 Journal of pharmacopuncture Vol.9 No.2

Objective : The purpose of this study was to compare and analyze the effect of ILIT and ELIT according to sex, age, disease on Hypercholesterolemia. Methods : The 73 patients who were irradiated to the Intravascular Laser and the 57 patients who were irradiated to the Extravascular Laser from 1999 to 2005 in Sam-se oriental medicine hospital were the study group. They were irradiated two or three times a week and checked total cholesterol before and after ten times of irradiation. The checked total cholesterol point, 150-199 were normal, 200-239 were borderline, over 240 were abnormal. They were classified into male and female(43 persons, 87 persons), adult and 01d(62 persons, 68 persons), cardiovascular disease and the other diseases(70 persons, 60 persons). Results : 1. Male, adult, and the other diseases groups were more effective in cholesterol reduction and in convertible change of each group than female, old, and cardiovascular disease groups, but only the convertible change of each group had statistic significance. 2. In male, old, and cardiovascular disease, ELIT was more effective than ILIT in cholesterol reduction and convertible change of each group, but this result didn't have statistic significance. 3. In female, ELIT was more effective than ILIT in cholesterol change but this result didn't have statistic significance. ILIT was more effective than ELIT in convertible change of each group and this result had statistic significance. 4. In adult and the other diseases, ILIT was more effective in cholesterol reduction, but this result didn't have statistic significance. ILIT was more effective than ELIT in convertible change of each group too and this result had statistic significance. Conclusion: It was thought to be undesirable to decide which is more effective treatment, but to be more desirable to choose one of ILIT or ELIT according to sex, age, disease than to decide it indiscriminately.

열전형 전압변환기의 모델링 및 교류-직류 변환 차이 계산 프로그램 개발

이정남,권성원,정재갑,이효경,박종권,Lee Jung-Nam,Kwon Sung-Won,Jung Jae-Kap,Lee Hyo-Kyoung,Park Jong-Kweon 한국전자파학회 2005 한국전자파학회논문지 Vol.16 No.5

본 논문에서는 손실이 있는 전송선로의 전달 임피던스를 적용한 열전형 전압 변환기(TVC)의 모델을 제안하였으며, TVC의 교류-직류 변환 차이를 계산하기 위한 프로그램을 개발하였다. 제안된 TVC는 열전소자(TE)와 열전소자의 측정범위를 확대하기 위한 저항선이 동축형 실린더의 중심에 직선형태로 직렬로 연결되었다. 제안된 TVC의 교류-직류 변환 차이를 실린더의 직경, 열전도도 등의 특성을 적용하여 시뮬레이션 하였다. 시뮬레이션 값이 불확도 이내에서 측정값과 잘 일치하였다. 일치도는 주파수 $40Hz\~10kHz$에서는 $1{\mu}V/V$이하였고, $20kHz\~100kHz$에서는 $5{\mu}V/V$이하였으며, $200kHz\~1MHz$에서는 최대 $12{\mu}V/V$이하의 차이를 보였다. We have proposed a model of a thermal voltage converter(TVC) using the trans-impedance of lossy transmission line and developed a calculation program for ac-dc transfer difference of the TVC. The proposed TVC consists of a thermo-element(f) and a range resistor wire connected in series at the center of coaxial cylinder. The range resistor wire is used to extend the measurement range of the n. The calculated ac-dc transfer differences of the proposed TVC by simulation agree well with the measured values within the uncertainties. The simulated and measured values are agreed within $1{\mu}V/V$ from 40 Hz to 10kHz, within $5{\mu}V/V$ from 20kHz to 100MHz, and within $12{\mu}V/V$ from 200kHz to 1 MHz.

TCD를 이용한 정상군과 중풍원인질환군의 혈류측정에 관한 비교연구

허정은,권정남,신원탁,김종득,이상희,손연희,김영균,Heo, Jeong-Eun,Kwon, Jung-Nam,Shin, Won-Tak,Kim, Jong-Deuk,Lee, Sang-Hee,Son, Yeoun-Hui,Kim, Young-Kyun 대한한방내과학회 2007 大韓韓方內科學會誌 Vol.28 No.2

Objectives : The purpose of this study was to evaluate the blood flow using doppler ultrasound of the MCA, ACA, PCA, BA, ICA in the patients with risk factor for stroke. Methods : 110 patients with risk factor for stroke were selected who had hypertension, diabetes mellitus, hyperlipidemia, or heart disease, as well as 89 healthy adults who did not have any symptoms of those diseases. To evaluate the blood flow, the Vs and Vm of the MCA, ACA, PCA, BA, and ICA in the two groups were measured. Result : In normal healthy adults, subjects showed a decrease in the Vs and Vm: with advancing in age, there was a significant difference in the Vs of MCA, PCA, BA, and ICA. There was a significant difference in the Vm of MCA, PCA, BA, and ICA. In normal healthy adults, females showed high velocities in all examined vessels. There was a significant difference in the Vs of BA. There was a significant difference in the Vm of BA, and ICA. There was a decrease in the Vs, and Vm of all examined vessels of patients with risk factors for stroke in comparison with normal healthy adults. There was no significant difference in the Vs. There was a significant difference in the Vm of MCA, ACA, and PCA. Results were the same between patients aged under 50 and total patients in the Vs and Vm of examined vessels. There were differences between patients aged over 50 and total patients in the Vs of MCA, PCA, BA and for patients aged over 50; patients with risk factor for stroke who had hypertension, diabetes mellitus, hyperlipidemia, or heat disease has higher Vs than healthy adults. Conclusions : There was a significant difference in the blood flow velocity between patients with risk factors for stroke and healthy adults. However, this result was different from results of comparison of TCD findings between patients and healthy individuals by age. Therefore, more detailed studies about aged patients are needed.

소복축어탕(小腹逐瘀湯)이 흰쥐 흉부대동맥의 혈관운동성에 미치는 영향

정용식,권정남,민성순,김종득,이상희,김재규,허정은,김영균,Jung, Yong-Sik,Kwon, Jung-Nam,Min, Sung-Soon,Kim, Jong-Deuk,Lee, Sang-Hee,Kim, Jae-Kyu,Heo, Jeong-Eun,Kim, Young-Kyun 대한한방내과학회 2006 大韓韓方內科學會誌 Vol.27 No.3

This study was carried out to examine the relaxational response to the water extract of Sobokchuko-tang (SCT) in rat thoracic aorta. Segments of thoracic aorta obtained from rats immediately after delivery were mounted in organ baths superfused on a polygraph. It was found that the thoracic aorta segments responded to the SCT with dose-dependent vasorelaxation. At $10^{-7}M$ phenylephrine-induced contraction, the contractile response of thoracic aortic rings were inhibited by 99.2%. 75.3%. 42.4% and 35.8% after addition of the respective concentration (1O. 30. 100. $300{\mu}g$/ml) of SCT. This vasorelaxation of rat thoracic aorta was endothelium-dependent. The action mechanisms of vasorelaxation are thought to be concerned with the role of NO. cAMP and cyclooxygenase. but not with cGMP.

족저 내측부의 동정맥 기형: 증례 보고

하대호,권정남,김유미,이준형,Ha, Dae-Ho,Kwon, Jung-Nam,Kim, Yu-Mi,Lee, Jun-Hyung 대한족부족관절학회 2016 대한족부족관절학회지 Vol.20 No.4

Arteriovenous malformation (A-V malformation) is defined as an abnormal connection between arteries and veins that lead to A-V shunting with an intervening network of vessels. A-V malformation is a rare condition, and spontaneous regression is also rare. A-V malformation becomes symptomatic when the surrounding tissue and osseous structures are negatively affected. A-V malformation has a high recurrence rate and is relatively hard to treat. In this case, a huge mass with pulsatile and bruit on the medial plantar area were observed. With the diagnosis of A-V malformation in accordance with the results from ultrasonography, magnetic resonance imaging and computed tomography angiography, and mass excision with feeding vessel ligation through plantar midfoot approach was completed successfully.

소속명탕(小續命湯)이 백서(白鼠)의 국소뇌혈류량(局所腦血流量)과 혈압(血壓)에 미치는 영향(影響)

김영균,권정남,김경민,조은희,박지은,Kim, Young-Kyun,Kwon, Jung-Nam,Kim, Kyung-Min,Cho, Eun-Hee,Park, Ji-Eun 대한한방내과학회 2000 大韓韓方內科學會誌 Vol.21 No.5

SosokMyeongTang(SMT) have been used in oriental medicine for many centuries as a therapeutic agent for cerebral disease. The effects of SMT on the vascular system is not known. The purpose of this study was to investigate the effects of SMT on the changes in blood pressure(BP) and regional cerebral blood flow(rCBF) of rats. SMT consists of the following components : Radix Ledebouriellae(防風), Radix Cocculi or Stephaniae(防己), Semen Armeniacae(杏仁), Cortex Cinnamomi(肉桂), Radix Scutellariae(黃芩), Radix Paeoniae Lactiflorae(白芍藥), Radix Ginseng(人蔘), Rhizoma Cnidll(川芎), Herba Ephedrae(麻黃), Radix Glycyrrhizae(甘草), Radix Aconiti(附子?), Fructus Zizyphi Jujubae(大棗), Rhizoma Zingiberis(生薑) and the changes of BP and rCBF were tested Leser-Doppler Flowmetry(LDF) The experimental results were as follows ; BP was not affected by SMT in rats, but rCBF was increased significantly by SMT in a dose dependent manner. SMT increased previous decreasd rCBF due to pretreatment methylene blue, but did not increased previous decreasd rCBF due to pretreatment L-NNA, indomethacin. Pretreatment with indomethacin decreased BP compaired with control group. These results suggest that SMT causes a diverse response of blood pressure and regional cerebral blood flow(rCBF). The increased rCBF is mediated by nitric oxide synthease.

뇌경색환자(腦梗塞患者)의 중풍전조증(中風前兆症)에 대한 임상적(臨床的) 고찰(考察)

서창훈,권정남,Seo, Chang-Hoon,Kwon, Jung-Nam 대한한방내과학회 1998 大韓韓方內科學會誌 Vol.19 No.1

Background : The purpose of this study was to investigate the prodromal of cerebral infarction in attacked patients and to prove the traditional hypothesis that some symptoms were to be prodromes of cerebral infarction in the oriental medicine. Methods : The questionnaire which was based on symptoms of traditional hypothesis was distributed cerebral infarction patients who were confirmed by Brain CT or MRI.. Results : Fifty-six patients(93.3%) felt some symptoms within three years before onset. Most common prodromal symptoms was dizziness(50%), failure of memory(45%), numbness of arm(45%). Conclusion : This results suggest that the prodromal symptoms before cerebral infarction can be regarded as predicting sign. and we think that these research may contributed to preventing stroke and relapse.