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석순자 ( Soon Ja Seok ),진용주 ( Yong Joo Jin ),유기범 ( Ki Bum Yoo ),홍승범 ( Seung Beom Hong ),권순우 ( Soon Wu Kwon ),김수진 ( Soo Jin Kim ),김정선 ( Jeong Seon Kim ) 한국균학회 2015 韓國菌學會誌 Vol.43 No.4
A re-study was conducted based on morphological characters of 39 specimens known as Mycena stylobates, collected from 33 areas, Mt. Chiack, Kangwon-do, etc. from 1988 to 2015 and deposited in the Herbarium Conservation Center of the National Institute of Agricultural Sciences (HCCN). All specimens cited here were confirmed as a new to science, and designated as Mycena yangsupensiae which was described and illustrated in detail. Mycelial growth of four cultures from the fruiting bodies of Mycena sp. (KACC54181, KACC54182, KACC54183, and KACC54184) was high on the potato dextrose agar (PDA), at 25oC. These four taxa were confirmed as the same species, Mycena yangsupensiae, by the internal transcribed spacer (ITS) sequencing analysis. All collections cited here are deposited in HCCN, Suwon.
한국산 광대버섯속 Lepidella 아속의 미기록종 보고
진용주 ( Yong Ju Jin ),유기범 ( Ki Bum Yoo ),홍승범 ( Seung Beom Hong ),권순우 ( Soon Wu Kwon ),김수진 ( Soo Jin Kim ),김성환 ( Seong Hwan Kim ),석순자 ( Soon Ja Seok ) 한국균학회 2015 韓國菌學會誌 Vol.43 No.4
Amanita subgenus Lepidella is a well-known group that includes lethal amanitas. However, the taxonomic investigation of Korean Amanita species is still in progress. In this study, we collected Amanita specimens in Korea from 2009 to 2015 and classified 22 of them as subgenus Lepidella based on the morphology. Phylogenetic analysis of the 22 Amanita specimens based on the internal transcribed spacers sequences identified 16 phylogenetic species which included three undescribed Amanita species (A. sepiacea, A. modesta and A. kotohiraensis). Microscopic features of newly recorded Amanita species were photographed and line drawings were made. All collections cited here are deposited in the Herbarium Conservation Center of the National Institute of Agricultural Sciences.
Violacein을 생산하는 Massilia sp. EP15224 균주
윤상홍 ( Sang Hong Yoon ),백희진 ( Hee Jin Baek ),권순우 ( Soon Wu Kwon ),이창묵 ( Chang Muk Lee ),심준수 ( Joon Soo Sim ),한범수 ( Bum Soo Hahn ),구본성 ( Bon Sung Koo ) 한국미생물생명공학회(구 한국산업미생물학회) 2014 한국미생물·생명공학회지 Vol.42 No.4
비올라세인은 항균, 항암, 항산화, 항말라리아, 항설사 활성과 같은 다양한 생리활성을 가지는 보라색소 물질로 알려져 있다. 본 연구에서는 한국의 산림토양에서 분리되었으며 보라색 색소를 생산하는 EP15224 균주를 선발하여 16S rRNA유전자의 염기서열을 분석하였다. 그 결과 Massilia sp. BS-1과 97%의 가장 높은상동성을 보였다. 또한 16S rRNA 유전자에 근거한 계통분류학적 분석에서 EP15224는 기존 보고된 비올라세인 생산 세균들과는 별개로 구분되는 Massilia속의 새로운 종임을 확인하였다. 이 균주가 생산하는 보라색소물질을 분리하여 LC/MS/MS로 분석한 결과, 분자량이 343.34인 비올라세인과 일치하였다. 또한 비올라세인의 생산효율을 높여주는 최적의 배지성분[glucose 2 g/l, (NH4)2SO4 1 g/l, Na2HPO4· 7H2O 2 g/l, KH2PO4 1 g/l, MgSO4· 7H2O0.1 g/l, L-tryptophan 0.24 g/l]과 배양조건[25oC에서 72시간배양, 250 rpm, 10% 접종량]을 조사하였으며 이 조건에서 액체배양 하였을 때 리터 당 280 mg의 비올라세인이 생산되었다. Violacein has received much attention due to its various important biological activities, including broad-spectrum antibacterial and antifungal activity, anti-malarial, anti-tumoral, anti-oxidant, and anti-diarrheal activities. EP15224 strain isolated from forest soils in Korea was found to be a new species belonged to the genus Massilia based on its 16S ribosomal DNA sequences. The 16S ribosomal DNA of strain EP15224 displayed 97% homology with Massilia sp. BS-1, the nearest violacein-producing bacterium. Strain EP15224 produced bluish-purple pigment well in a synthetic MM2 medium containing glucose, (NH4)2SO4, Na2HPO4·7H2O, KH2PO4, MgSO4·7H2O, and 1 mM L-tryptophan. The chemical analysis of the pigment by LC/MS/MS showed that it is violacein with molecular weight of 343.34. This is the second report on the production of violacein by a Massilia species. In this study, the optimal culture conditions for violacein production were established under which 280 mg/l crude violacein was produced : glucose 2 g/l, (NH4)2SO4 1 g/l, Na2HPO4·7H2O 2 g/l, KH2PO4 1 g/l, MgSO4·7H2O 0.1 g/l, L-tryptophan 0.24 g/l, 25 ml medium in a 250 ml flask, with an inoculumn size of 10% (v/v), 72 h of cultivation with 250 rpm at 25oC.