돼지 간장 조직내 Protein Methylase Ⅱ 저해제의 정제 및 특성

권명희,정기경,이회영,이향우,홍성렬 ( Myung Hee Kwon,Ki Kyung Jung Hoi,Young Lee,Hyang Woo Lee,Sungyoul Hong ) 생화학분자생물학회 1994 BMB Reports Vol.27 No.6

An inhibitor for protein methylase II (EC 2.1.1.24) was solubilized from porcine liver microsomal fraction by heat treatment, and purified by ultrafiltration, Sephadex G-25 chromatogrnphy, and HPLC using μ-Bondapak C_(18) column. The purified inhibitor was near homogeniety as judged by HPLC. The molecular weight of the inhibitor was estimated to be 1,676 Da by analysis of amino acid composition. And it was revealed that the inhibitor molecule is rich in alanine and glycine. The activity of the inhibitor was not affected by heat treatment up to 100℃ as well as hydrolytic enzymes. The K; value for the protein methylase II which has been purified from porcine spleen was measured to be 1.3 × 10^(-7) M. Inhibition studies showed that the inhibitor was noncompetitive with respect to S-adenosyl-L-methionine (SAM) and activities of several SAM-dependent methylases were also inhibited by the purified inhibitor.

하악골 전돌증 환자에서 하악지 시상분할골 절단술 적용술 후 초기 안정성 평가

권명희,임대호,백진아,신효근,고승오,Kwon, Myung-Hee,Leem, Dae-Ho,Baek, Jin-A,Shin, Hyo-Keun,Ko, Seung-O 대한악안면성형재건외과학회 2011 Maxillofacial Plastic Reconstructive Surgery Vol.33 No.3

Purpose: The purpose of this study is to evaluate the post-operative skeletal stability after surgical correction of patients with mandibular prognathism by bilateral sagittal split ramus osteotomy (BSSRO) and to evaluate the horizontal relapse tendency after the surgery. Methods: Twenty-six patients with Class III dental and skeletal malocclusion were selected for this retrospective study. Fifteen of them underwent BSSRO for mandibular setback and eleven of them underwent two-jaw surgery (Lefort I and BSSRO). In each patient, lateral cephalometric radiographs were taken pre-operatively, post-operatively within 1 week, and post-operatively after eight months. After tracing of the cephalometric radiographs, various parameters were measured. The analyses were done by linear measurement to evaluate the change in position of hard tissue B point, pogonion and mandibular plan angle by examination on lateral cephalograms. Results: The horizontal relapse rate was 27.1% at B point and 31.6% at pogonion in patients who underwent BSSRO. The horizontal relapse rate of the group where the amount of correction exceeded 10 mm was 25.69% at B point. Conclusion: There were no statistical differences on the magnitude of setback and direction of rotation of the mandible in mandibular stability. There were also no statistical differences between single mandibular surgery and two-jaw surgery for mandibular stability.

A Patient with Cleft Lip Combined with Branchial Cleft Cyst : A Rare Case Report

권명희,임대호,고승오,신효근,Kwon, Myung-Hee,Leem, Dae-Ho,Ko, Seung-O,Shin, Hyo-Keun Korean Cleft Lip and Plate Association 2010 대한구순구개열학회지 Vol.13 No.1

구순구개열은 두경부에서 가장 흔히 발생하는 선천적 기형 중 하나로 554명 중 1명의 빈도로 나타나며 인종에 따라 다양하다. 구순구개열 환아들은 다른 선천적 기형을 동반하여 나타나는 경우가 흔하며, 그 빈도는 문헌에 따라 다르지만 1.5~63.4%로 나타난다. 새열낭종은 두번째 인두강의 폐쇄부전으로 나타나는 선천적 결손으로, 주로 흉쇄유돌근 전방에 나타난다. 구순구개열 환자에 있어 새열낭종을 동반하는 경우는 매우 드물다. 전북대학교 구강악안면외과학 교실에서는 새열낭종을 동반한 구순열 환아 1례를 경험하였다. 환아는 우측 불완전 편측성 구순열로 내원하여, 구순성형술과 함께 우측 목에 존재하던 새열낭종에 대한 제거 수술을 시행하였다. 환아는 출생 당시부터 심실중격결손 및 동맥관개존증 등의 선천적 심장질환 및 갑상선 기능저하증을 가지고 있던 환아로 다양한 선천적인 결손을 동반한 본 환아의 증례를 문헌고찰과 함께 보고하는 바이다.

브롬화난연제 및 브롬화다이옥신류의 연구동향

권명희(Myung-Hee Kwon),송기봉(Ki-Bong Song),강영렬(Yung-Ryul Kang),황승률(Seung-Ryul Hwang),신선경(Sun Kyoung Shin),김금희(Kum-Hee Kim),박진수(Jin Soo Park),김수진(Sue-Jin Kim),이수영(Su-Yung Lee),김동훈(Dong-Hoon Kim),정광용(Kwang-Y 한국분석과학회 2008 분석과학 Vol.21 No.6

고도경제성장과 주택 정책

권명희(Kwon Myung-Hee) 서울행정학회 1998 한국사회와 행정연구 Vol.9 No.1

반대측 경부 림프절로 전이된 초기 설암의 치험례

권명희(Myung-Hee Kwon),김성일(Sung-Il Kim),김평수(Pyoung-Soo Kim),임대호(Dae-Ho Leem),신효근(Hyo-Keun Shin),고승오(Seung-O Ko) 대한구강악안면외과학회 2009 대한구강악안면외과학회지 Vol.35 No.5

Likely to be the most common oral cancer, squamous cell carcinoma(SCC) of the tongue accounts for about 20% of all oral and pharyngeal cancers. SCC of the tongue frequently arises in the lateral border, and if it metastasize, it occurs on submandibular gland and neck lymph nodes. Location of the primary lesions and neck lymph node metastasis affect the prognosis and decrease survival rate of patients with carcinoma of the tongue. The authors experienced the patient with contralateral neck lymph node metastasis of SCC of the tongue. The patient came to our department with chief complaint of elevated lesion on left lateral border of the tongue. The mass was diagnosed as T2N0M0, Stage II invasive SCC of oral tongue. Computed tomography(CT) & magnetic resonance imaging(MRI) which were taken before the operation showed no significant finding of metastasis. Surgical mass removal and preventive neck dissection on the left side were done. While follow up PET/CT, contralateral neck lymph node metastasis(right side, level II) was detected, and re-operation(Rt. side RND) was done. There are few studies concerning the contralateral neck lymph node metastasis related with SCC of the tongue. The purpose of this report is to introduce the uncommon case of contralateral neck lymph node metastasis occurred in the T2-stage of SCC of the tongue treated by surgical resection.

결혼이주여성의 국제의료관광코디네이터 활성화 방안 연구

권명희(Kwon, Myung Hee) 충북대학교 국제개발연구소 2016 사회적경제와 정책연구 Vol.1 No.1

본 연구는 전 세계적으로 신성장 동력산업으로 주목받고 있는 의료관광을 목적으로 한국을 방문하는 국제 의료관광객 100만 명의 시대를 맞이하여, 현 재 의료관광에 관련된 기업에 채용되어 있는 결혼이주여성 전문인력들은 한 국의 의료관광에 대한 기대와 국제의료관광코디네이터에 대해 어떠한 생각들 을 하고 있는지 살펴 본 것이다. 연구를 위해 서울·대전지역에서 활동하고 있는 의료관광 전문인력 중에서 중국, 일본, 베트남을 국적으로 두고 있는 결 혼이주여성 185명을 대상으로 설문 조사를 실시하였다. 연구의 결과 요약은 다음과 같다. 첫째, 의료관광 업체들이 서울을 중심으로 집중되어 있었고, 중 증 환자를 대상으로 하는 병원보다는 성형외과와 피부과에 큰 비중을 차지하 고 있으므로, 다양한 진료과목이 선택되도록 의료관광 관련 기관과 여행사, 의료관광에이전시 등이 협력하여 균형적인 지역 선정이 요구된다. 둘째, 최신 의 의료관광 정보확보를 위해 표본조사대상을 대인면접 조사법과 설문지기법 을 병행하여 실시해야 한다. 셋째 외국인 환자에 대한 의료서비스 제공을 위 해 의료인력 이외에도 마케팅, 통역, 고객서비스, 프로그램 개발 등 국제적인 수준의 업무를 수행할 수 있는 전문인력 양성이 필요하다. This study aims to take a look into the expectation of the medical tourism of Korea and the thought about international medical tourism coordinators that immigrant married women professionals who are employed companies related to medical tourism have. Because the medical tourism is expected to be " a new growth engine industry" all over the world and international tourists who visit Korea for the medical tourism are nearly a million. In order to this study, a survey was conducted on 185 immigrant married women of medical tourism professionals in Seoul and Daejeon, and they are from China, Japan or Vietnam. The results of the study are as follows. First, most medical tourism businesses cluster in Seoul, and the plastic surgery and the dermatology clinics are more than hospitals for patients. So balanced areas have to be selected through the cooperation among medical tourism companies, travel agencies, and medical tourism agencies. Second, in order to secure the latest information about medical tourism, sample surveys should be taken through interviews and questionnaires. Third, in order to provide medical services for foreigners, the professional trainings for marketing, interpretations, customer care services, program developments and so on should be prepared.

울산시 신주거지의 아파트 계획을 위한 거주자 주거선택행동에 관한 연구

김선중,권명희,Kim Sun-Joong,Kwon Myung-Hee 한국주거학회 2006 한국주거학회 논문집 Vol.17 No.4

The purpose of the study was to identify the level of housing choice behavior by using the factors of residential satisfaction level, mobility motivation, and housing needs of potential purchasers in Bukgu New Housing Area, Ulsan. The survey used questionnaire from 326 households living in Bukgu and nearby area and analyzed using descriptive statistics. The research results showed low degree of residential satisfaction in storage space and neighborhood. The mobility motivations were improvement of physical environment improvement, education environment, walking road, view and lighting. The housing needs for indoor spaces showed to want more functional arrangement than the room size. The housing needs for outdoor spaces showed to want neighborhood environment in connection with the education or hospital facilities, the welfare facilities for pre-kindergarten children and elders and the leisure facilities. And the housing needs for facilities were floor furnishing for health, crime prevention system for safety needs, housekeeping appliance against environmental pollution, additional function for energy saving. The housing needs for common spaces showed that the residents preferred playing facilities by age group, exercise facilities, the community hall and the rest room which can do games or meetings.

Ribosome Display를 이용한 항체선별 방법의 확립

이명신,권명희,김경민,박선,신호준,김형일,Lee, Myung-Shin,Kwon, Myung-Hee,Hwang Kim, Kyongmin,Park, Sun,Shin, Ho-Joon,Kim, Hyung-Il 대한면역학회 2003 Immune Network Vol.3 No.3

Background: Phage display is the most widely used technique among display methods to produce monoclonal antibody fragment with a specific binding activity. Having a large library for efficient antibody display/selection is quite laborious process to have more than $10^9$ members of transformants. To overcome these limitations, several in vitro selection approaches have been reported. Ribosome display that links phenotypes, proteins, directly to genotype, mRNA, is one of the in vitro display methods. Ribosome display can reach the size of scFv library up to $10^{14}$ molecules and it can be further diversified during PCR steps. To select the high affinity scFv from one pot library, we established ribosome display technique by modifying the previously reported eukaryotic translation system. Methods: To establish the antibody selection system by ribosome display, we used 3D8, anti-DNA antibody. A 3D8 scFv was synthesized in vitro by an in vitro transcription-translation system. The translated 3D8 scFv and the encoding 3D8 mRNA are connected to the ribosome. These scFv-ribosome-mRNA complexes were selected by binding to their specific antigens. The eluted mRNAs from the complexes are reverse transcribed and re-amplified by PCR. To apply this system, antibody library from immunized mouse with terminal protein (TP)-peptide of hepatitis B virus DNA polymerase TP domain was also used. This TP-peptide encompasses the 57~80 amino acid residues of TP. These mRNA/ribosome/scFv complexes by our system were panned three times against TP-peptide. The enrichment of antibody from library was determined by radioimmunoassay. Results: We specifically selected 3D8, anti-DNA antibody, against ssDNA as a model system. The selected 3D8 RNAs sequences from translation complexes were recovered by RT-PCR. By applying this model system, we enriched TP-peptide-specific scFv pools through three cycles of panning from immunized library. Conclusion: We show that our translating ribosome complexes are well maintained and we can enrich the TP-specific scFv pools. This system can be applied to select specific antibody from an antibody library.

부엌가구디자인 효율화를 위한 식생활물품 체크리스트

김선중,권명희,Kim, Sun-Joong,Kwon, Myung-Hee 한국주거학회 2010 한국주거학회 논문집 Vol.21 No.3

The purpose of this study is to develop a checklist based on the stored items of cooking, for making a good kitchen furniture reseach. The research candidates are purposive sampled from 30 households of for 40 or 50 pyeong-type apartments in Kangnam, Seoul, The research data are is collected gotten at by a field study of the stored items of cooking, eating and utility area, by an in-depth interview to 30 households, and by a field survey of the department stores, super-markets of home appliances. Studied households in average hold about 890 items, which are classified as 239 sorts. The first step of making the checklist is to categorize items into 9 biggest categories, considering the usage of items and the stage of the preparing the food. when the item was used. Second step of making the checklist is to categorize the items into 31 smaller categories, which reflect the shape of items, the storage style and the place of storage. Third step of making the checklist is to sort the items into smallest categories by the frequency of the usage, storage type, and the additional capacity of the item. Even Although items are sorted into the same level of categories, the frequency of use make a different storage area. Also, the storage style of the items was different according to the storage area. Based on these factors, we suggested a checklist based on the stored items of cooking.