기관지 폐포 세정액에서 뉴우모시스티스 카리니의 면역세포화학적 검출

권건영,조승제,김상표,박관규,장은숙,김정숙,Kwon, Kun-Young,Cho, Seung-Che,Kim, Sang-Pyo,Park, Kwan-Kyu,Chang, Eun-Sook,Kim, Chung-Sook 대한세포병리학회 1997 대한세포병리학회지 Vol.8 No.1

Pneumocystis carinli is an established cause of pulmonary infections in immuno-compromised hosts. Several cytoiogical stains, such as Papanicolaou, Gomori methenamine sliver(GMS) and Diff-Quik have been used for detection of the organism, but occasionally can be laborious and, due to a degree of nonspecificity, may be misleading. We evaluated the diagnostic utility of immunocytochenmical stains that recognize P. carinii in bornchoalveolar lavage from experimentally Induced P. carinii pneumonia rats(n=15). In audition to routine stains for diagnosis by morphologic recognition of P. carinii on Papanicolaou, GMS and Diff-Quik stains, bronchoalveolar lavage samples were reacted with immunocytochemical stains using monoclonal antibodies(MAB) 092 and 902. In bronchoalveolar lavage P. carinii organisms were detected In 9 of 10 cases(90%) using each MAB 092 and 902, whereas GMS and Diff-Quik stains demonstrated P. carinii in 13(86%) and 11(73%) of 15 cases respectively. In lung tissue specimens(n=15) P. carinii organisms were well identified on GMS stain and immunohistochemical stains using MAB 092 and 902 in ail cases. We believe that the immunocytochemical staining using MAB 092 and/or 902 is a very useful and diagnostic tool In addition to GMS and Diff-Qulk stain to detect P. carinii organisms in bronchoalveolar lavage.

유방의 침윤성 미세유두모양 암종의 세침흡인 세포학적 소견 - 4예 보고 -

권선영,정혜라,강유나,김상표,권건영,이상숙,Kwon, Sun-Young,Jung, Hae-Ra,Kaug, Yu-Na,Kim, Sang-Pyo,Kwon, Kun-Young,Lee, Sang-Sook 대한세포병리학회 2004 대한세포병리학회지 Vol.15 No.2

Invasive micropapillary carcinoma (IMPCa) is a rare variant of invasive ductal carcinoma of the breast. This variant is associated with a set of peculiar cytological findings and aggressive biological behaviors. In most reported cases, IMPCa has involved massive axillary lymph node metastases at the time of diagnosis. We experienced four cases of cytological features of IMPCa, all of which were verified by histological examination. Fine needle aspiration cytology (FNAC) revealed malignant epithelial cells, which formed small, oval to angulated papillary clusters, which lacked central fibrovascular cores. The histological findings of the four cases revealed both pure and mixed forms of IMPCa, composed of cohesive malignant epithelial cells, surrounded by distinctive clear spaces and separated by thin fibrous septa. All patients evidenced axillary lymph node metastases at the time of diagnosis. It is important to identify the peculiar cytological findings which would differentiate IMPCa from other diseases.

기관지 폐포 세정액의 세포학적 소견

권건영,조승제,박관규,장은숙,Kwon, Kun-Young,Cho, Seung-Che,Park, Kwan-Kyu,Chang, Eun-Sook 대한세포병리학회 1990 대한세포병리학회지 Vol.1 No.2

Bronchoalveolar lavage (BAL) has emerged as a useful technique for the study of pulmonary interstitial disorders. Several types of Information are provided by the evaluation of lavage fluid identification of cellular constituents helps to separate inflammatory process. Recently we have studied cellular constituents of BAL from three cases with histologically confirmed pulmonary sarcoidosis, idiopathic pulmonary fibrosis and hypereosinophilic syndrome. Pulmonary sarcoidosis showed a marked increase in lymphocytes, idiopathic pulmonary fibrosis revealed a predominance of neutrophils, and hypereosinophilic syndrome presented a marked increase in eosinophils in the lavage fluids.

Pneumocystis carinii 폐렴의 기관지 폐포세정액: 세포학적 및 전자현미경적 소견

권건영,윤철희,김상표,박관규,장은숙,Kwon, Kun-Young,Yun, Cheol-Hee,Kim, Sang-Pyo,Park, Kwan-Kyu,Chang, Eun-Sook 대한세포병리학회 1994 대한세포병리학회지 Vol.5 No.1

The cytological and ultrastructural findings of Pneumocystis carinii(PC) obtained from rats by bronchoalveolar lavage (BAL) are described. All developmental forms of the PC organisms were obtained in the lavage fluid. Papanicolaou stain revealed conglomeration of PC as a foamy cast. The cystic walls of PC were well identified on Gomori's methenamine silver stain. Trophozoites and intracystic bodies were stained by Giemsa and Diff-Quik techniques. Some PC organisms were seen within the alveolar macrophages. Ultrastructurally, the cysts were almost circular in shape, and were nearly devoid of surface tubular extensions. The wall of the cyst was composed of an unit membrane, an intermediate electron lucent layer and an external electron dense layer The cysts frequently contained intracystic bodies, so called sporozoites. Occasionally empty or collapsed cysts with no intracystic bodies, and precysts were found. Trophozoites were variable in size and shape with abundant tubular extensions along the single electron dense pellicle. BAL is a useful method for concentrating the various morphologic forms of PC organisms, and is a rapid diagnostic method for PC pneumonia.

PAM 염색으로 본 AIIoxan 당뇨가토의 신상피 세포의 Lysosome

정상봉,권건영,손태중 慶北大學校 醫科大學 1980 慶北醫大誌 Vol.21 No.1

저자는 백색 수놈 가토에 체중 1kg당 130mg의 alloxan을 투여하여 alloxan 당뇨 가토를 만든 후, 3개월 및 6개월째에 신상피세포내에 출현하는 lysosome을 PAM 염색으로 검색하여 얻은 성적을 요약하면 다음과 같다. alloxan 당뇨 가토에 있어서 신상피세포에 있어서는 lysosome은 일반적으로 증가하는 경향을 나타내며, 이 증가는 3개월째보다 6개월째에 더욱 현저하다. 증가된 lysosome은 그 구조상으로 보다 secondary lysosome이 많으며, PAM염색으로 이들은 모두 양성으로 나타나며, 그 전자밀도는 primary lysosome보다는 secondary lysosome이 낮은 경향을 나타낸다. 이상의 연구성적으로 보아 alloxan 당뇨 가토에 있어서 신상피에는 lysosome이 증가하며, 이들 내용물은 PAM 염색으로 보다 당단백이 많으며, 그 농도는 secondary lysosome이 primary lysosome보다 낮은 경향이다. The author have studied morphological changes of the lysosomes in the renal tubular epithelium of diabetic rabbits induced by intravenous administration of l30mg of alloxan/kg of body weight with PAM stian in the third and sixth month after induction of diabetes. The results were summarized as follow. The lysosomes in the renal tubular epithelium of alloxan-diabetic rabbits tended to increase in number and it was more pronounced in the sixth month after induction of diabetes than in the third month. Ultrastructurally, most lysosomes were secondary ones in nature and revealed positive reaction for PAM stain. The electron density tended to more decrease in the secondary lysosomes than in the primary ones. From the basis of these results, in the renal tubular epithelium of alloxan-induced diabetic rabbits the lysosomes were increased in number and their contents were composed largely of glycoprotein of which concentration was lower in the Secondary lysosome than in the primary ones.

관류, 보존 및 재관류 과정이 이식된 개의 폐조직에 미치는 영향

임영근 ( Young Keun Lim ),박창권 ( Chang Kwon Park ),권건영 ( Kun Young Kwon ) 대한결핵 및 호흡기학회 1999 Tuberculosis and Respiratory Diseases Vol.46 No.4

선천성 양측성 신경모세포종의 치료경험 1례

서연경,김흥식,권건영,이희정,구홍회,Seo, Yeon Kyong,Kim, Heung Sik,Kwon, Kun Young,Lee, Hee Jung,Koo, Hong Hoe 대한소아청소년과학회 2003 Clinical and Experimental Pediatrics (CEP) Vol.46 No.12

저자들은 산전 초음파에서 복부 종괴가 발견되어 관찰 도중 복부 팽만과 호흡 부전이 발생하여 방사선요법과 항암요법으로 치료하였던 양측성 선천성 신경모세포종 1례를 문헌 고찰과 함께 보고하는 바이다. 선천성 양측성 신경모세포종의 경우 임상경과를 잘 관찰하고 치료여부를 결정하여야 할 것으로 생각된다. Neuroblastoma is the most common intraabdominal malignant tumor of childhood, with 40% arising from the adrenal gland. Bilateral adrenal involvement from synchronous development or metastatic spread of tumor is rarely seen in children with neuroblastoma. The patient was born with a spontaneous vaginal delivery. Birth weight was 3,200 g. Fetal ultrasonography showed a left adrenal cystic mass. At two weeks of age, she was admitted due to a massive abdominal distension and tachypnea. Percutaneous ultrasonography guided biopsy of the left adrenal mass was performed. The result of the biopsy was neuroblastoma. Vincristine and cyclophosphamide were administerd intravenously and 450 cGy of irradiation was added. Left adrenalectomy was accomplished and postoperative course was uneventful. The patient received cancer chemotherapy with a combination of carboplatin, ifosfamide and VP-16 and is now being followed up for three months. We have experienced a case of congenital bilateral neuroblastoma and report the case with brief review of related literatures.

폐의 림프상피종양 암종

정치영 ( Chi Young Jung ),심상우 ( Sang Woo Shim ),박창권 ( Chang Kwon Park ),권건영 ( Kun Young Kwon ),전영준 ( Young June Jeon ) 대한결핵 및 호흡기학회 2011 Tuberculosis and Respiratory Diseases Vol.71 No.5

Lymphoepithelioma-like carcinoma (LELC) of the lung is a very rare tumor. Originally described in the nasopharynx as lymphoepithelioma, this carcinoma has also been found in the stomach, esophagus, thymus, cervix, urinary bladder, skin, and salivary glands. Histologically, it is an undifferentiated carcinoma that has a syncytial appearance with tumor cells and is infiltrated by numerous lymphocytes, macrophages, and plasma cells. LELC of the lung occurs more commonly in Asians, particularly Chinese. Many studies have reported the association between Epstein-Barr virus (EBV) and LELC of the lung in Asian patients. A 45-year-old man had a solitary pulmonary nodule on a routine chest X-ray examination. As a malignant tumor was suspected, surgical resection was performed to establish the correct diagnosis. The pathology of the excised tumor demonstrated LELC of the lung. This is the first report of LELC of the lung in Korea.

부르크막 위에 이식된 망막색소상피세포의 조직형태에 따른 배양양상비교

김광수,김유철,권건영,Kwang-Soo Kim,M,D,Yu-Cheol Kim,M,D,Kun-Young Kwon,M,D 대한안과학회 2005 대한안과학회지 Vol.46 No.3

Purpose: To compare the cultured morphology of retinal pigment epithelium (RPE) cells which were transplanted onto the Bruch`s membrane (BM) in different tissue types. Methods: Cultured porcine RPE cells were harvested in three types of transplants: single cell (SC) suspension, cell cluster (CC) suspension and cell sheet (CS). After RPE cell transplants were plated onto the porcine BM explants at three different cell concentrations, they were dissected and examined with a transmission electron microscope and a scanning electron microscope at 1 day, 3 days, and 1, 2 and 4 weeks for morphological study. Results: All types of RPE transplants were grown and proliferated well on BM and required a shorter time to reach confluence with higher cell concentration. Although CC transplants took a little longer to reach confluence on BM than SC transplants, they were nevertheless well grown on BM and showed good cellular morphology in monolayer. The time to confluence was much longer for the CS transplants than for the SC and CC transplants and the proliferated cells tended to be large, flat and to have scanty microvilli on the cell surface with reaching peripheral portion of confluent cell layers. Conclusions: CC suspension may be a better candidate for RPE transplantation in the case of using cultured RPE cells as transplant.

폐의 거대세포암종의 세포학적 소견

윤철희,배지연,김상표,권건영,김정숙,장은숙,Yun, Cheol-Hee,Bae, Ji-Yeon,Kim, Sang-Pyo,Kwon, Kun-Young,Kim, Chung-Sook,Chang, Eun-Sook 대한세포병리학회 1994 대한세포병리학회지 Vol.5 No.2

Pulmonary giant cell carcinoma is one of the most highly malignant neoplasms of the lung. Although mixed malignant glandular or squamous components may be associated with a giant cell carcinoma, it is a distinct clinical and morphologic entity. We reviewed cytologic presentations of 6 cases of pulmonary giant ceil carcinoma. Cytologically, the single most characteristic feature of giant cell carcinoma was an extremely large, bizarre cancer cell engulfing numerous leukocytes. The nuclei of these cells showed occasional prominent nucleoli, and the cytoplasm was abundant. Giant cells were also seen in other types of pulmonary carcinoma, but the slant cells of this neoplasm could be differentiated from those encountered in undifferentiated large cell carcinoma and squamous cell carcinoma by the abundant cytoplasm, the presence of markedly enlarged nuclei, prominent nucleoli, and a significant degree of phagocytosis. In conclusion, precise diagnosis and classification of lung cancer is imperative because of proved correlation between cell type and prognosis.