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Enzyme-Linked Immunosorbent Assay(ELISA)를 이용한 혈청 및 원유 중의 Mycobacterium bovis 항체 검출에 관한 연구
심항섭 ( Hang Sub Shim ),국정희 ( Jung Hee Kook ),박병옥 ( Byoung Ok Park ),김성열 ( Sung Youl Kim ),박유순 ( Yu Soon Park ) 한국가축위생학회 1997 韓國家畜衛生學會誌 Vol.20 No.2
In order to supplement a diagnostic method for detection of infectious cattle to bovine tuberculosis, performed ELISA for detection of antibody to M bovis in serum and milk. The diagnostic efficacy of the established ELISA was compared with test of the tuberculin skin test for bovine tuberculosis. The positive corresponding rate of serum ELISA and tuberculin skin test showed 84.3%, milk ELISA and tuberculin skin test showed 75.0%, milk ELISA and serum ELISA showed 75.0% respectively. Comparison of the serum and milk to tuberculin antibody concentration in tuberculin positive cattle, the milk contained 1/100-1/150 concentration compared serum tuberculin concentration. The established ELISA was considered efficient for detection of antibodies to M bovis in serum and milk.
심항섭 ( Hang Sub Shim ),국정희 ( Jung Hee Kook ),황영옥 ( Young Ok Hwang ),정봉수 ( Bong Su Jung ),김학열 ( Hak Yeol Kim ),이모란 ( Mo Ran Lee ),유성종 ( Sung Jong Yoo ),강순근 ( Sun Kun Kang ),임경애 ( Kyung Ae Lim ),고태오 ( Ta 한국가축위생학회 1998 韓國家畜衛生學會誌 Vol.21 No.3
Since bovine leukosis caused considerable economic loss to the dairy industry, seroepidemiological survey on bovine leukosis was carried out for the dairy herds in Kyunggi province. 1. When compared the results of immunodifussion test with those of enzyme-linked immunosor-bent assay(ELISA) for 94 dairy herds sera, the relationship between the immunodifussion test and ELISA were showen high corresponding rate with sensitivity(97.5%) and specificity(92.6%). 2. In immunodiffusion test for bovine leukosis virus (BLV) antibody in 570 dairy cattle from 30 herds, mean positive rate for BLV antibody was 28.2 %. The positive rate by districts were 16.5% in central, 35.4% in east, 17.3% in west, 29.1% in south, 31.6% in north, 43.7% in northeast. 3. When the results of serological studies was analyzed by age groups, the number of positive was increased gradually with the advanced in age of herds. The highest positive rate was found in the age over 6 years. 4. Of 30 dairy herds examined, 5 herds(16.7%) have no reactions against BLV antigen while 15 herds (50%) showed the range of 1~5 positive cattle and 5 herds(16.7%), the rang of over 11 positive cattle.
효소면역법을 이용한 Brucella abortus 항체 검출에 관한 연구
심항섭 ( Hang Sub Shim ),국정희 ( Jung Hee Kook ),정봉수 ( Bong Su Chung ),고태오 ( Tae Oh Ko ),조중현 ( Jung Hyun Cho ),박유순 ( Yu Soon Park ) 한국가축위생학회 1998 韓國家畜衛生學會誌 Vol.21 No.2
In order to establish a rapid, sensitive and specific diagnostic method for detection of antibody to Brucella abortus, a enzyme-linked immunosorbent assay(ELISA) was adapted. The diagnostic efficacy of the established ELISA was compared with that of the standard tube agglutination test for B abortus. 1. It was found that the optimal concentration of antigen for this ELISA was 51ug/m1, the optimal dilution of conjugate was 1:2000, and the optimal dilution of serum was 1:200, respectively. 2. Cut off value in this ELISA was 1,102 that was determined by mean absorbance(at 492nm) of tube agglutination test negative serum added with the triple value of the standared devation. 3. The relationship between the tube agglutination test and ELISA was showen high corresponding rate with sensitivity(96.3%) and specificity(98.1%). 4. The efficacy of the ELISA for detection of B abortus antibody was compared with tube agglutination test in brucellosis outbreak farm. The sensivity of ELSIA was higher than tube agglutination test.
경기지역의 우결핵 발생에 관한 역학적 고찰(1987~1996)
우종태 ( Jong Tae Woo ),심항섭 ( Hang Sub Shim ),고태오 ( Tae Oh Ko ),국정희 ( Jung Hee Kook ),박유순 ( Yu Soon Park ),조중현 ( Jung Hyun Chi ) 한국가축위생학회 1998 韓國家畜衛生學會誌 Vol.21 No.1
As the result of epidemiological analysis on bovine tuberculosis in Kyonggi province during the last ten years(1987-1996), 1. The annual incidence number of bovine tuberculosis positive farms in Kyonggi province fell down from 81 in 1987 to 14 in 1989. But the incidence is increased since 1990, in 1995 the incidence number was 102 farms of the most incidence. Total number of bovine tuberculosis positive farms were 456 farms(56.7%) in Kyonggi province during the 1987-1996 period. 2. The developmental trends of bovine tuberculosis positive heads was similiar to that of positive farms. Since 1993, the incidence was suddenly increased. Total number of bovine tuberculosis positive heads were 1,015 head(64.3%) in Kyonggi province during the 10 years. 3. Average incidence for rate during the last ten years(1987-1996) was 0.10%. The positive rate was suddenly increased since 1993 and in 1996, the positive rate was 0.23% of the highest incidence. 4. Regional incidence number of bovine tuberculosis positive heads in Kyonggi province during the last seven years(1990-1996) was the most in Hwasung, Pochon, Pyungtek in order of incidence and bovine tuberculosis positive density was 1.297% of the highest in Buchon. 5. In 244 farms(82.4%), bovine tuberculosis first occurred during the last seven years (1990-1996) but in farms over second occurrence, the number of bovine tuberculosis positive heads were 520 heads(58.2%).