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RISS 인기검색어
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- 저자 : 구용범(Y. B. Koo) (검색결과 2 건)
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추백리가 감염된 닭의 간에서 발현이 증가하는 APOA1 단백질의 확인
정기철,이유주,유성란,이준헌,장병귀,구용범,소현경,최강덕,Jung K. C.,Lee Y. J.,Yu S. L.,Lee J. H.,Jang B. K.,Koo Y. B.,So H. K.,Choi K. D. 한국가금학회 2005 韓國家禽學會誌 Vol.32 No.1
가금 추백리와 밀접한 관계가 있는 단백질을 동정하기 위하여 인위적으로 추백리를 유도한 개체와 정상인 개체의 간에서 단백질을 추출하였으며, 2차원 전기영동(2DE)과 mass spectrometry(MS)를 이용하여 차등 발현되는 단백질을 조사하였다. 총 300여개의 단백질 spot들이 관찰되었으며, 이중 발현양에 현저한 차이를 보이는 spot을 MALDI-TOF MS와 protein database search를 통해 분석한 결과, 가금 APOAI (Apolipoprotein A1)으로 확인되었다. 추백리가 감염된 닭의 간에서 APOA1 단백질의 증가는 손상된 혈관의 재생과 밀접한 관계가 있으며 추백리의 감염 여부를 나타내 주는 Bio-marker로서 활용될 수 있을 것으로 사료된다. The aim of this study was to investigate differentially expressed proteins between normal chicken liver and chicken liver inffeted by Salmonella pullorum. 2-dimensional electrophoresis (2DE) and mass spectrometry (MS) were used to identify the proteins. More than 300 protein spots were detected on silver stained 2DE gels using pH 3$\~$10 gradients. The most outstanding protein spot was further analyzed by MALDI-TOF MS and protein database using the Mascot search engine. The protein was finally identified as APOAI (Apolipoprotein AI). Based on the known function of the APOAI, this gene acts protective action against the accumulation of platelet thrombin at the site of vascular damage for the pullorum disease. Therefore APOAI protein, identified in this study, can be a valuable biomarker in relation to the pullorum disease in chicken.
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한우에 있어서 발정주기중 혈중 Progesterone 및 Insulin - like Growth Factor-I 농도의 변화
성환후,우제석,임석기,고응규,백광수,박진기,구용범,이장형 ( H . H . Seong,J . S . Woo,S . K . Im,Y . G . Ko,K . S . Baek,J . K . Park,Y . B . Koo,J . H . Lee ) 한국축산학회 1997 한국축산학회지 Vol.39 No.3
The objective of this study was to determine changes in circulating progesterone and insulinlike growth factor-I(IGF-I) concentrations during estrous cycles in Hanwoo. The serum progesterone and IGF-I concentrations during the estrous cycles in the apparently healthy normal cycles and freemartins of 670 days old were measured with a commercial Radio Immuno Assay(RIA) kit. Serum progesterone concentration was low from Day 1 to 3(about 0.25ng/㎖) and then increased gradually to 9.3ng/㎖ by Day 17, and decreased dramatically by Day 21 of the estrous cycle. However, serum progesterone concentration of freemartins was almost undetectable during the same periods of normal Hanwoo. Serum IGF-I concentrations from Day 3 to 16 of normal estrous cycle were higher than those of early luteal stage( Day 1∼2) and luteal regression stage (Day 19∼20). The lowest serum IGF-I concentration was observed on Day 21. In the freemartin of Hanwoo, serum IGF-I concentrations were 1/4 of those in the normal cycles. It is suggested that the serum progesterone concentration is closely related to the serum IGF-I concentration in Hanwoo.
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